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Protein

Forkhead transcription factor HCM1

Gene

HCM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor regulating the cell cycle specific transcription of a spindle pole body (SPB) calmodulin binding protein SPC110. Required for full induction of SPC110 transcription in late G1. Binds to DNA consensus sequence 5'-[AT]AA[TC]AAACAA[AT]-3'. Dosage dependent suppressor of calmodulin mutants which have specific defects in SPB assembly.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi108 – 19992Fork-headPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: SGD

GO - Biological processi

  1. cellular response to oxidative stress Source: SGD
  2. mitochondrion organization Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  4. spindle pole body organization Source: SGD
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29368-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead transcription factor HCM1
Alternative name(s):
High-copy suppressor of calmodulin protein 1
Gene namesi
Name:HCM1
Ordered Locus Names:YCR065W
ORF Names:YCR65W, YCR902
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome III

Organism-specific databases

SGDiS000000661. HCM1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nuclear chromatin Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi155 – 1551N → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-159; A-162 and A-163. 1 Publication
Mutagenesisi159 – 1591H → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-162 and A-163. 1 Publication
Mutagenesisi162 – 1621S → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-163. 1 Publication
Mutagenesisi163 – 1631L → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-162. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Forkhead transcription factor HCM1PRO_0000091902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei342 – 3421Phosphothreonine1 Publication
Modified residuei496 – 4961Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by CDK1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25364.
PaxDbiP25364.

Expressioni

Gene expression databases

GenevestigatoriP25364.

Interactioni

Protein-protein interaction databases

BioGridi31041. 171 interactions.
DIPiDIP-4993N.
IntActiP25364. 5 interactions.
MINTiMINT-531907.
STRINGi4932.YCR065W.

Structurei

3D structure databases

ProteinModelPortaliP25364.
SMRiP25364. Positions 109-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5025.
GeneTreeiENSGT00780000121868.
HOGENOMiHOG000142335.
InParanoidiP25364.
KOiK09413.
OMAiDASWQNS.
OrthoDBiEOG7647BS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS_1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNEDISIID GHNSFLTEKS TVLLTQAKRT LEDEKEMITP PSSTVRKTMK
60 70 80 90 100
EVNKRPSHPL SPDHSSPIAP SKAKRQRSDT CARSNGNLTL EEILQSLERR
110 120 130 140 150
RINGELAKKP PYSYATLICL AILQSQEGKL TLSQIYHWIH VHFPYYKQKD
160 170 180 190 200
ASWQNSIRHN LSLNDAFIKT EKSCDGKGHF WEVRPGAETK FFKGENRGYE
210 220 230 240 250
FVKDSLQDIG KYFEIDSTLD ELEQVESGEG NDDLPDEEER EEAGKFPSIE
260 270 280 290 300
IQLNSSPILR VSQLHHIPQL KTDNSVLNPH ENLESMRNMI ENDVNNIDSL
310 320 330 340 350
EPPYVMKKYH TSLGLPSLVN AKDHFQAGVK NNNITQANRF NTLPITSAKS
360 370 380 390 400
PQNFRKYFTS FNSNFEDLSP LRSNVGAGSL LDPLPYSPLK LYDQKNLALM
410 420 430 440 450
SKPQSQQSYS NSQLPPPPSS HGSDLLKTPK MRHSDGLEKT PSRLISTPKD
460 470 480 490 500
GNSILRKWQT PSHLFEDLYC SPLFRAIETP IRYITTPGGT LETQISPRKS
510 520 530 540 550
SAPDVLTSAT NSKFASSGLF GVDVYSVWKR ATEKISDGNN TTDSNQKHHP
560
YHNHPSNDSG NEKN
Length:564
Mass (Da):63,647
Last modified:October 4, 2010 - v3
Checksum:i0BF083C2617C0DCD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1302KL → NV in AAA34665 (PubMed:8441413).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08252 Genomic DNA. Translation: AAA34665.1.
X59720 Genomic DNA. Translation: CAA42280.2.
BK006937 Genomic DNA. Translation: DAA07537.1.
PIRiS22262.
RefSeqiNP_009991.2. NM_001178776.1.

Genome annotation databases

EnsemblFungiiYCR065W; YCR065W; YCR065W.
GeneIDi850429.
KEGGisce:YCR065W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08252 Genomic DNA. Translation: AAA34665.1.
X59720 Genomic DNA. Translation: CAA42280.2.
BK006937 Genomic DNA. Translation: DAA07537.1.
PIRiS22262.
RefSeqiNP_009991.2. NM_001178776.1.

3D structure databases

ProteinModelPortaliP25364.
SMRiP25364. Positions 109-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31041. 171 interactions.
DIPiDIP-4993N.
IntActiP25364. 5 interactions.
MINTiMINT-531907.
STRINGi4932.YCR065W.

Proteomic databases

MaxQBiP25364.
PaxDbiP25364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYCR065W; YCR065W; YCR065W.
GeneIDi850429.
KEGGisce:YCR065W.

Organism-specific databases

SGDiS000000661. HCM1.

Phylogenomic databases

eggNOGiCOG5025.
GeneTreeiENSGT00780000121868.
HOGENOMiHOG000142335.
InParanoidiP25364.
KOiK09413.
OMAiDASWQNS.
OrthoDBiEOG7647BS.

Enzyme and pathway databases

BioCyciYEAST:G3O-29368-MONOMER.

Miscellaneous databases

NextBioi966009.

Gene expression databases

GenevestigatoriP25364.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS_1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A dosage-dependent suppressor of a temperature-sensitive calmodulin mutant encodes a protein related to the fork head family of DNA-binding proteins."
    Zhu G., Muller E.G.D., Amacher S.L., Northrop J.L., Davis T.N.
    Mol. Cell. Biol. 13:1779-1787(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces cerevisiae."
    Benit P., Chanet R., Fabre F., Faye G., Fukuhara H., Sor F.
    Yeast 8:147-153(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Valles G., Volckaerts G.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The fork head transcription factor Hcm1p participates in the regulation of SPC110, which encodes the calmodulin-binding protein in the yeast spindle pole body."
    Zhu G., Davis T.N.
    Biochim. Biophys. Acta 1448:236-244(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASN-155; HIS-159; SER-162 AND LEU-163, RECOGNITION OF DNA-BINDING SITE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: PHOSPHORYLATION BY CDK1.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-342 AND SER-496, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHCM1_YEAST
AccessioniPrimary (citable) accession number: P25364
Secondary accession number(s): D6VR68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1992
Last sequence update: October 4, 2010
Last modified: March 31, 2015
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.