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Protein

Forkhead transcription factor HCM1

Gene

HCM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor regulating the cell cycle specific transcription of a spindle pole body (SPB) calmodulin binding protein SPC110. Required for full induction of SPC110 transcription in late G1. Binds to DNA consensus sequence 5'-[AT]AA[TC]AAACAA[AT]-3'. Dosage dependent suppressor of calmodulin mutants which have specific defects in SPB assembly.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi108 – 199Fork-headPROSITE-ProRule annotationAdd BLAST92

GO - Molecular functioni

GO - Biological processi

  • cellular response to oxidative stress Source: SGD
  • mitochondrion organization Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • spindle pole body organization Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29368-MONOMER.
ReactomeiR-SCE-156711. Polo-like kinase mediated events.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead transcription factor HCM1
Alternative name(s):
High-copy suppressor of calmodulin protein 1
Gene namesi
Name:HCM1
Ordered Locus Names:YCR065W
ORF Names:YCR65W, YCR902
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR065W.
SGDiS000000661. HCM1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear chromatin Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi155N → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-159; A-162 and A-163. 1 Publication1
Mutagenesisi159H → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-162 and A-163. 1 Publication1
Mutagenesisi162S → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-163. 1 Publication1
Mutagenesisi163L → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-162. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000919021 – 564Forkhead transcription factor HCM1Add BLAST564

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei342PhosphothreonineCombined sources1
Modified residuei496PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CDK1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25364.
PRIDEiP25364.

PTM databases

iPTMnetiP25364.

Interactioni

Protein-protein interaction databases

BioGridi31041. 173 interactors.
DIPiDIP-4993N.
IntActiP25364. 5 interactors.
MINTiMINT-531907.

Structurei

3D structure databases

ProteinModelPortaliP25364.
SMRiP25364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00790000123003.
HOGENOMiHOG000142335.
InParanoidiP25364.
KOiK09413.
OMAiWISVHFP.
OrthoDBiEOG092C0D3W.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. Fork_head_dom.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNEDISIID GHNSFLTEKS TVLLTQAKRT LEDEKEMITP PSSTVRKTMK
60 70 80 90 100
EVNKRPSHPL SPDHSSPIAP SKAKRQRSDT CARSNGNLTL EEILQSLERR
110 120 130 140 150
RINGELAKKP PYSYATLICL AILQSQEGKL TLSQIYHWIH VHFPYYKQKD
160 170 180 190 200
ASWQNSIRHN LSLNDAFIKT EKSCDGKGHF WEVRPGAETK FFKGENRGYE
210 220 230 240 250
FVKDSLQDIG KYFEIDSTLD ELEQVESGEG NDDLPDEEER EEAGKFPSIE
260 270 280 290 300
IQLNSSPILR VSQLHHIPQL KTDNSVLNPH ENLESMRNMI ENDVNNIDSL
310 320 330 340 350
EPPYVMKKYH TSLGLPSLVN AKDHFQAGVK NNNITQANRF NTLPITSAKS
360 370 380 390 400
PQNFRKYFTS FNSNFEDLSP LRSNVGAGSL LDPLPYSPLK LYDQKNLALM
410 420 430 440 450
SKPQSQQSYS NSQLPPPPSS HGSDLLKTPK MRHSDGLEKT PSRLISTPKD
460 470 480 490 500
GNSILRKWQT PSHLFEDLYC SPLFRAIETP IRYITTPGGT LETQISPRKS
510 520 530 540 550
SAPDVLTSAT NSKFASSGLF GVDVYSVWKR ATEKISDGNN TTDSNQKHHP
560
YHNHPSNDSG NEKN
Length:564
Mass (Da):63,647
Last modified:October 5, 2010 - v3
Checksum:i0BF083C2617C0DCD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129 – 130KL → NV in AAA34665 (PubMed:8441413).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08252 Genomic DNA. Translation: AAA34665.1.
X59720 Genomic DNA. Translation: CAA42280.2.
BK006937 Genomic DNA. Translation: DAA07537.1.
PIRiS22262.
RefSeqiNP_009991.2. NM_001178776.1.

Genome annotation databases

EnsemblFungiiCAA42280; CAA42280; CAA42280.
YCR065W; YCR065W; YCR065W.
GeneIDi850429.
KEGGisce:YCR065W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08252 Genomic DNA. Translation: AAA34665.1.
X59720 Genomic DNA. Translation: CAA42280.2.
BK006937 Genomic DNA. Translation: DAA07537.1.
PIRiS22262.
RefSeqiNP_009991.2. NM_001178776.1.

3D structure databases

ProteinModelPortaliP25364.
SMRiP25364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31041. 173 interactors.
DIPiDIP-4993N.
IntActiP25364. 5 interactors.
MINTiMINT-531907.

PTM databases

iPTMnetiP25364.

Proteomic databases

MaxQBiP25364.
PRIDEiP25364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42280; CAA42280; CAA42280.
YCR065W; YCR065W; YCR065W.
GeneIDi850429.
KEGGisce:YCR065W.

Organism-specific databases

EuPathDBiFungiDB:YCR065W.
SGDiS000000661. HCM1.

Phylogenomic databases

GeneTreeiENSGT00790000123003.
HOGENOMiHOG000142335.
InParanoidiP25364.
KOiK09413.
OMAiWISVHFP.
OrthoDBiEOG092C0D3W.

Enzyme and pathway databases

BioCyciYEAST:G3O-29368-MONOMER.
ReactomeiR-SCE-156711. Polo-like kinase mediated events.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.

Miscellaneous databases

PROiP25364.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. Fork_head_dom.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHCM1_YEAST
AccessioniPrimary (citable) accession number: P25364
Secondary accession number(s): D6VR68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 5, 2010
Last modified: November 30, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.