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Protein

Forkhead transcription factor HCM1

Gene

HCM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor regulating the cell cycle specific transcription of a spindle pole body (SPB) calmodulin binding protein SPC110. Required for full induction of SPC110 transcription in late G1. Binds to DNA consensus sequence 5'-[AT]AA[TC]AAACAA[AT]-3'. Dosage dependent suppressor of calmodulin mutants which have specific defects in SPB assembly.1 Publication

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi108 – 199Fork-headPROSITE-ProRule annotationAdd BLAST92

GO - Molecular functioni

GO - Biological processi

  • anatomical structure morphogenesis Source: GO_Central
  • cell differentiation Source: GO_Central
  • cellular response to oxidative stress Source: SGD
  • mitochondrion organization Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • spindle pole body organization Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29368-MONOMER
ReactomeiR-SCE-156711 Polo-like kinase mediated events
R-SCE-198693 AKT phosphorylates targets in the nucleus
R-SCE-211163 AKT-mediated inactivation of FOXO1A
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-69273 Cyclin A/B1/B2 associated events during G2/M transition

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead transcription factor HCM1
Alternative name(s):
High-copy suppressor of calmodulin protein 1
Gene namesi
Name:HCM1
Ordered Locus Names:YCR065W
ORF Names:YCR65W, YCR902
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR065W
SGDiS000000661 HCM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi155N → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-159; A-162 and A-163. 1 Publication1
Mutagenesisi159H → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-162 and A-163. 1 Publication1
Mutagenesisi162S → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-163. 1 Publication1
Mutagenesisi163L → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-162. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000919021 – 564Forkhead transcription factor HCM1Add BLAST564

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei342PhosphothreonineCombined sources1
Modified residuei496PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CDK1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25364
PaxDbiP25364
PRIDEiP25364

PTM databases

iPTMnetiP25364

Interactioni

Protein-protein interaction databases

BioGridi31041, 283 interactors
DIPiDIP-4993N
IntActiP25364, 6 interactors
MINTiP25364
STRINGi4932.YCR065W

Structurei

3D structure databases

ProteinModelPortaliP25364
SMRiP25364
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00920000148947
HOGENOMiHOG000142335
InParanoidiP25364
KOiK09413
OMAiWISVHFP
OrthoDBiEOG092C0D3W

Family and domain databases

CDDicd00059 FH, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR001766 Fork_head_dom
IPR018122 TF_fork_head_CS_1
IPR030456 TF_fork_head_CS_2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00250 Forkhead, 1 hit
PRINTSiPR00053 FORKHEAD
SMARTiView protein in SMART
SM00339 FH, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00657 FORK_HEAD_1, 1 hit
PS00658 FORK_HEAD_2, 1 hit
PS50039 FORK_HEAD_3, 1 hit

Sequencei

Sequence statusi: Complete.

P25364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNEDISIID GHNSFLTEKS TVLLTQAKRT LEDEKEMITP PSSTVRKTMK
60 70 80 90 100
EVNKRPSHPL SPDHSSPIAP SKAKRQRSDT CARSNGNLTL EEILQSLERR
110 120 130 140 150
RINGELAKKP PYSYATLICL AILQSQEGKL TLSQIYHWIH VHFPYYKQKD
160 170 180 190 200
ASWQNSIRHN LSLNDAFIKT EKSCDGKGHF WEVRPGAETK FFKGENRGYE
210 220 230 240 250
FVKDSLQDIG KYFEIDSTLD ELEQVESGEG NDDLPDEEER EEAGKFPSIE
260 270 280 290 300
IQLNSSPILR VSQLHHIPQL KTDNSVLNPH ENLESMRNMI ENDVNNIDSL
310 320 330 340 350
EPPYVMKKYH TSLGLPSLVN AKDHFQAGVK NNNITQANRF NTLPITSAKS
360 370 380 390 400
PQNFRKYFTS FNSNFEDLSP LRSNVGAGSL LDPLPYSPLK LYDQKNLALM
410 420 430 440 450
SKPQSQQSYS NSQLPPPPSS HGSDLLKTPK MRHSDGLEKT PSRLISTPKD
460 470 480 490 500
GNSILRKWQT PSHLFEDLYC SPLFRAIETP IRYITTPGGT LETQISPRKS
510 520 530 540 550
SAPDVLTSAT NSKFASSGLF GVDVYSVWKR ATEKISDGNN TTDSNQKHHP
560
YHNHPSNDSG NEKN
Length:564
Mass (Da):63,647
Last modified:October 5, 2010 - v3
Checksum:i0BF083C2617C0DCD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129 – 130KL → NV in AAA34665 (PubMed:8441413).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08252 Genomic DNA Translation: AAA34665.1
X59720 Genomic DNA Translation: CAA42280.2
BK006937 Genomic DNA Translation: DAA07537.1
PIRiS22262
RefSeqiNP_009991.2, NM_001178776.1

Genome annotation databases

EnsemblFungiiCAA42280; CAA42280; CAA42280
YCR065W; YCR065W; YCR065W
GeneIDi850429
KEGGisce:YCR065W

Similar proteinsi

Entry informationi

Entry nameiHCM1_YEAST
AccessioniPrimary (citable) accession number: P25364
Secondary accession number(s): D6VR68
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 5, 2010
Last modified: June 20, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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