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P25364 (HCM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Forkhead transcription factor HCM1
Alternative name(s):
High-copy suppressor of calmodulin protein 1
Gene names
Name:HCM1
Ordered Locus Names:YCR065W
ORF Names:YCR65W, YCR902
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor regulating the cell cycle specific transcription of a spindle pole body (SPB) calmodulin binding protein SPC110. Required for full induction of SPC110 transcription in late G1. Binds to DNA consensus sequence 5'-[AT]AA[TC]AAACAA[AT]-3'. Dosage dependent suppressor of calmodulin mutants which have specific defects in SPB assembly. Ref.6

Subcellular location

Cytoplasm. Nucleus Ref.6 Ref.7.

Post-translational modification

Phosphorylated by CDK1. Ref.9

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 fork-head DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564Forkhead transcription factor HCM1
PRO_0000091902

Regions

DNA binding108 – 19992Fork-head Ref.6

Amino acid modifications

Modified residue3421Phosphothreonine Ref.10
Modified residue4961Phosphoserine Ref.10

Experimental info

Mutagenesis1551N → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-159; A-162 and A-163. Ref.6
Mutagenesis1591H → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-162 and A-163. Ref.6
Mutagenesis1621S → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-163. Ref.6
Mutagenesis1631L → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-162. Ref.6
Sequence conflict129 – 1302KL → NV in AAA34665. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25364 [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: 0BF083C2617C0DCD

FASTA56463,647
        10         20         30         40         50         60 
MMNEDISIID GHNSFLTEKS TVLLTQAKRT LEDEKEMITP PSSTVRKTMK EVNKRPSHPL 

        70         80         90        100        110        120 
SPDHSSPIAP SKAKRQRSDT CARSNGNLTL EEILQSLERR RINGELAKKP PYSYATLICL 

       130        140        150        160        170        180 
AILQSQEGKL TLSQIYHWIH VHFPYYKQKD ASWQNSIRHN LSLNDAFIKT EKSCDGKGHF 

       190        200        210        220        230        240 
WEVRPGAETK FFKGENRGYE FVKDSLQDIG KYFEIDSTLD ELEQVESGEG NDDLPDEEER 

       250        260        270        280        290        300 
EEAGKFPSIE IQLNSSPILR VSQLHHIPQL KTDNSVLNPH ENLESMRNMI ENDVNNIDSL 

       310        320        330        340        350        360 
EPPYVMKKYH TSLGLPSLVN AKDHFQAGVK NNNITQANRF NTLPITSAKS PQNFRKYFTS 

       370        380        390        400        410        420 
FNSNFEDLSP LRSNVGAGSL LDPLPYSPLK LYDQKNLALM SKPQSQQSYS NSQLPPPPSS 

       430        440        450        460        470        480 
HGSDLLKTPK MRHSDGLEKT PSRLISTPKD GNSILRKWQT PSHLFEDLYC SPLFRAIETP 

       490        500        510        520        530        540 
IRYITTPGGT LETQISPRKS SAPDVLTSAT NSKFASSGLF GVDVYSVWKR ATEKISDGNN 

       550        560 
TTDSNQKHHP YHNHPSNDSG NEKN 

« Hide

References

« Hide 'large scale' references
[1]"A dosage-dependent suppressor of a temperature-sensitive calmodulin mutant encodes a protein related to the fork head family of DNA-binding proteins."
Zhu G., Muller E.G.D., Amacher S.L., Northrop J.L., Davis T.N.
Mol. Cell. Biol. 13:1779-1787(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces cerevisiae."
Benit P., Chanet R., Fabre F., Faye G., Fukuhara H., Sor F.
Yeast 8:147-153(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"The fork head transcription factor Hcm1p participates in the regulation of SPC110, which encodes the calmodulin-binding protein in the yeast spindle pole body."
Zhu G., Davis T.N.
Biochim. Biophys. Acta 1448:236-244(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASN-155; HIS-159; SER-162 AND LEU-163, RECOGNITION OF DNA-BINDING SITE.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Targets of the cyclin-dependent kinase Cdk1."
Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O.
Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CDK1.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-342 AND SER-496, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08252 Genomic DNA. Translation: AAA34665.1.
X59720 Genomic DNA. Translation: CAA42280.2.
BK006937 Genomic DNA. Translation: DAA07537.1.
PIRS22262.
RefSeqNP_009991.2. NM_001178776.1.

3D structure databases

ProteinModelPortalP25364.
SMRP25364. Positions 109-191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31041. 169 interactions.
DIPDIP-4993N.
IntActP25364. 5 interactions.
MINTMINT-531907.
STRING4932.YCR065W.

Proteomic databases

MaxQBP25364.
PaxDbP25364.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR065W; YCR065W; YCR065W.
GeneID850429.
KEGGsce:YCR065W.

Organism-specific databases

SGDS000000661. HCM1.

Phylogenomic databases

eggNOGCOG5025.
GeneTreeENSGT00390000000589.
HOGENOMHOG000142335.
KOK09413.
OMADASWQNS.
OrthoDBEOG7647BS.

Enzyme and pathway databases

BioCycYEAST:G3O-29368-MONOMER.

Gene expression databases

GenevestigatorP25364.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSPR00053. FORKHEAD.
SMARTSM00339. FH. 1 hit.
[Graphical view]
PROSITEPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966009.

Entry information

Entry nameHCM1_YEAST
AccessionPrimary (citable) accession number: P25364
Secondary accession number(s): D6VR68
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 5, 2010
Last modified: May 14, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families