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P25364

- HCM1_YEAST

UniProt

P25364 - HCM1_YEAST

Protein

Forkhead transcription factor HCM1

Gene

HCM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Transcription factor regulating the cell cycle specific transcription of a spindle pole body (SPB) calmodulin binding protein SPC110. Required for full induction of SPC110 transcription in late G1. Binds to DNA consensus sequence 5'-[AT]AA[TC]AAACAA[AT]-3'. Dosage dependent suppressor of calmodulin mutants which have specific defects in SPB assembly.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi108 – 19992Fork-headPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: SGD
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. cellular response to oxidative stress Source: SGD
    2. mitochondrion organization Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. spindle pole body organization Source: SGD
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29368-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Forkhead transcription factor HCM1
    Alternative name(s):
    High-copy suppressor of calmodulin protein 1
    Gene namesi
    Name:HCM1
    Ordered Locus Names:YCR065W
    ORF Names:YCR65W, YCR902
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    SGDiS000000661. HCM1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nuclear chromatin Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi155 – 1551N → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-159; A-162 and A-163. 1 Publication
    Mutagenesisi159 – 1591H → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-162 and A-163. 1 Publication
    Mutagenesisi162 – 1621S → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-163. 1 Publication
    Mutagenesisi163 – 1631L → A: No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-162. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 564564Forkhead transcription factor HCM1PRO_0000091902Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei342 – 3421Phosphothreonine2 Publications
    Modified residuei496 – 4961Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by CDK1.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP25364.
    PaxDbiP25364.

    Expressioni

    Gene expression databases

    GenevestigatoriP25364.

    Interactioni

    Protein-protein interaction databases

    BioGridi31041. 170 interactions.
    DIPiDIP-4993N.
    IntActiP25364. 5 interactions.
    MINTiMINT-531907.
    STRINGi4932.YCR065W.

    Structurei

    3D structure databases

    ProteinModelPortaliP25364.
    SMRiP25364. Positions 109-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5025.
    GeneTreeiENSGT00390000000589.
    HOGENOMiHOG000142335.
    KOiK09413.
    OMAiDASWQNS.
    OrthoDBiEOG7647BS.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00250. Fork_head. 1 hit.
    [Graphical view]
    PRINTSiPR00053. FORKHEAD.
    SMARTiSM00339. FH. 1 hit.
    [Graphical view]
    PROSITEiPS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25364-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNEDISIID GHNSFLTEKS TVLLTQAKRT LEDEKEMITP PSSTVRKTMK    50
    EVNKRPSHPL SPDHSSPIAP SKAKRQRSDT CARSNGNLTL EEILQSLERR 100
    RINGELAKKP PYSYATLICL AILQSQEGKL TLSQIYHWIH VHFPYYKQKD 150
    ASWQNSIRHN LSLNDAFIKT EKSCDGKGHF WEVRPGAETK FFKGENRGYE 200
    FVKDSLQDIG KYFEIDSTLD ELEQVESGEG NDDLPDEEER EEAGKFPSIE 250
    IQLNSSPILR VSQLHHIPQL KTDNSVLNPH ENLESMRNMI ENDVNNIDSL 300
    EPPYVMKKYH TSLGLPSLVN AKDHFQAGVK NNNITQANRF NTLPITSAKS 350
    PQNFRKYFTS FNSNFEDLSP LRSNVGAGSL LDPLPYSPLK LYDQKNLALM 400
    SKPQSQQSYS NSQLPPPPSS HGSDLLKTPK MRHSDGLEKT PSRLISTPKD 450
    GNSILRKWQT PSHLFEDLYC SPLFRAIETP IRYITTPGGT LETQISPRKS 500
    SAPDVLTSAT NSKFASSGLF GVDVYSVWKR ATEKISDGNN TTDSNQKHHP 550
    YHNHPSNDSG NEKN 564
    Length:564
    Mass (Da):63,647
    Last modified:October 5, 2010 - v3
    Checksum:i0BF083C2617C0DCD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti129 – 1302KL → NV in AAA34665. (PubMed:8441413)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08252 Genomic DNA. Translation: AAA34665.1.
    X59720 Genomic DNA. Translation: CAA42280.2.
    BK006937 Genomic DNA. Translation: DAA07537.1.
    PIRiS22262.
    RefSeqiNP_009991.2. NM_001178776.1.

    Genome annotation databases

    EnsemblFungiiYCR065W; YCR065W; YCR065W.
    GeneIDi850429.
    KEGGisce:YCR065W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08252 Genomic DNA. Translation: AAA34665.1 .
    X59720 Genomic DNA. Translation: CAA42280.2 .
    BK006937 Genomic DNA. Translation: DAA07537.1 .
    PIRi S22262.
    RefSeqi NP_009991.2. NM_001178776.1.

    3D structure databases

    ProteinModelPortali P25364.
    SMRi P25364. Positions 109-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31041. 170 interactions.
    DIPi DIP-4993N.
    IntActi P25364. 5 interactions.
    MINTi MINT-531907.
    STRINGi 4932.YCR065W.

    Proteomic databases

    MaxQBi P25364.
    PaxDbi P25364.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR065W ; YCR065W ; YCR065W .
    GeneIDi 850429.
    KEGGi sce:YCR065W.

    Organism-specific databases

    SGDi S000000661. HCM1.

    Phylogenomic databases

    eggNOGi COG5025.
    GeneTreei ENSGT00390000000589.
    HOGENOMi HOG000142335.
    KOi K09413.
    OMAi DASWQNS.
    OrthoDBi EOG7647BS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29368-MONOMER.

    Miscellaneous databases

    NextBioi 966009.

    Gene expression databases

    Genevestigatori P25364.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00250. Fork_head. 1 hit.
    [Graphical view ]
    PRINTSi PR00053. FORKHEAD.
    SMARTi SM00339. FH. 1 hit.
    [Graphical view ]
    PROSITEi PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A dosage-dependent suppressor of a temperature-sensitive calmodulin mutant encodes a protein related to the fork head family of DNA-binding proteins."
      Zhu G., Muller E.G.D., Amacher S.L., Northrop J.L., Davis T.N.
      Mol. Cell. Biol. 13:1779-1787(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces cerevisiae."
      Benit P., Chanet R., Fabre F., Faye G., Fukuhara H., Sor F.
      Yeast 8:147-153(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "The fork head transcription factor Hcm1p participates in the regulation of SPC110, which encodes the calmodulin-binding protein in the yeast spindle pole body."
      Zhu G., Davis T.N.
      Biochim. Biophys. Acta 1448:236-244(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASN-155; HIS-159; SER-162 AND LEU-163, RECOGNITION OF DNA-BINDING SITE.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: PHOSPHORYLATION BY CDK1.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-342 AND SER-496, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHCM1_YEAST
    AccessioniPrimary (citable) accession number: P25364
    Secondary accession number(s): D6VR68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 358 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3