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P25360 (PHO87_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inorganic phosphate transporter PHO87
Gene names
Name:PHO87
Ordered Locus Names:YCR037C
ORF Names:YCR37C, YCR524
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length923 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the uptake of inorganic phosphate.

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

Present with 504 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the CitM (TC 2.A.11) transporter family. [View classification]

Contains 1 SPX domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 923923Inorganic phosphate transporter PHO87
PRO_0000172517

Regions

Topological domain1 – 461461Extracellular Potential
Transmembrane462 – 48221Helical; Potential
Topological domain483 – 49311Cytoplasmic Potential
Transmembrane494 – 51421Helical; Potential
Topological domain515 – 53723Extracellular Potential
Transmembrane538 – 55821Helical; Potential
Topological domain559 – 58325Cytoplasmic Potential
Transmembrane584 – 60421Helical; Potential
Topological domain605 – 62723Extracellular Potential
Transmembrane628 – 64821Helical; Potential
Topological domain649 – 66719Cytoplasmic Potential
Transmembrane668 – 68821Helical; Potential
Topological domain689 – 70719Extracellular Potential
Transmembrane708 – 72821Helical; Potential
Topological domain729 – 7357Cytoplasmic Potential
Transmembrane736 – 75621Helical; Potential
Topological domain757 – 76711Extracellular Potential
Transmembrane768 – 78821Helical; Potential
Topological domain789 – 80214Cytoplasmic Potential
Transmembrane803 – 82321Helical; Potential
Topological domain824 – 84926Extracellular Potential
Transmembrane850 – 87021Helical; Potential
Topological domain871 – 89828Cytoplasmic Potential
Transmembrane899 – 91921Helical; Potential
Topological domain920 – 9234Extracellular Potential
Domain1 – 334334SPX

Amino acid modifications

Modified residue2281Phosphoserine Ref.7
Glycosylation1621N-linked (GlcNAc...) Potential
Glycosylation2021N-linked (GlcNAc...) Potential
Glycosylation2741N-linked (GlcNAc...) Potential
Cross-link102Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7

Experimental info

Sequence conflict721S → P in CAA40229. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25360 [UniParc].

Last modified March 15, 2004. Version 2.
Checksum: E94DC4C152355A47

FASTA923102,540
        10         20         30         40         50         60 
MRFSHFLKYN AVPEWQNHYL DYNELKNLIY TLQTDELKQE TPTGDLNDDA DSQTPGPIAD 

        70         80         90        100        110        120 
IESNIAAGEP SSSKRRFTHK LKRKLFGSKT PSGSKRGDSD EKAIDGNNIN EETIELDELS 

       130        140        150        160        170        180 
PQGKTTSFNK NFIRKKFFES RSSSVSSEGK TLFSSYDTFV TNLSDEKLKV DDFYKRMEAK 

       190        200        210        220        230        240 
FYERFDHLIN DLEKEGIVTR LNETFNPEIQ ALPPLREIIS GTSETHSSNN PFEIHSSNID 

       250        260        270        280        290        300 
SELRNRFDYS EEEMDEDDDV DVFADTTDNT ALLNYSQFNI KSQKKSLLKQ TIINLYIDLC 

       310        320        330        340        350        360 
QLKSFIELNR MGFSKITKKS DKVLHMNTRQ ELIESEEFFK DTYIFQHETL SSLNSKIAQL 

       370        380        390        400        410        420 
IEFYAVLMGQ PGNVDSCKQE LKSYLHDHIV WERSNTWKDM LGLSSQNNDI ITIEDEAEKL 

       430        440        450        460        470        480 
MQEKLQIEYF KYPLPKPINL KFTKIENLAV PKLFFGKRAM KIGFIIIVTG VLLGVKTFND 

       490        500        510        520        530        540 
PVEHRCMALV ECCAFLWASE AIPLHITGLL VPLLTVLFRV LKDDDGKVMG AAAASTEILG 

       550        560        570        580        590        600 
TMWSSTIMIL LAGFTLGEAL SQYNVAKVLA SWLLALAGTK PRNVLLMAMS VVFFLSMWIS 

       610        620        630        640        650        660 
NVASPVLTYS LLTPLLDPLD YTSPFAKALV MGVALSADIG GMASPISSPQ NIISMQYLKP 

       670        680        690        700        710        720 
YGIGWGQFFA VALPTGILSM LCSWALMILT FKIGKTKLEK FKPIRTRFTI KQYFIIIVTI 

       730        740        750        760        770        780 
ATILLWCVES QIESAFGSSG EIAVIPIVLF FGTGLLSTKD FNTFPWSIVV LAMGGIALGK 

       790        800        810        820        830        840 
AVSSSGLLVT IARALQKKIQ NDGVFAILCI FGILMLVVGT FVSHTVSAII IIPLVQEVGD 

       850        860        870        880        890        900 
KLSDPKAAPI LVFGCALLAS CGMGLASSGF PNVTAISMTD KKGNRWLTVG AFISRGVPAS 

       910        920 
LLAFVCVITL GYGISSSVLK GST

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase."
Thierry A., Fairhead C., Dujon B.
Yeast 6:521-534(1990) [PubMed: 1964349] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed: 1574125] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 72.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae."
Bun-Ya M., Shikata K., Nakade S., Yompakdee C., Harashima S., Oshima Y.
Curr. Genet. 29:344-351(1996) [PubMed: 8598055] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-228, UBIQUITINATION AT LYS-102, MASS SPECTROMETRY.
Strain: SUB592.
[8]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X56909 Genomic DNA. Translation: CAA40229.1.
X59720 Genomic DNA. Translation: CAA42304.2.
BK006937 Genomic DNA. Translation: DAA07516.1.
PIRMMBY7C. S12919.
RefSeqNP_009966.2. NM_001178751.1.

3D structure databases

ProteinModelPortalP25360.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-7842N.
IntActP25360. 4 interactions.
MINTMINT-1362346.
STRINGP25360.

Protein family/group databases

TCDB2.A.47.2.1. divalent anion:Na+ symporter (DASS) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR037C; YCR037C; YCR037C.
GeneID850403.
KEGGsce:YCR037C.
NMPDRfig|4932.3.peg.691.

Organism-specific databases

CYGDYCR037c.
SGDS000000633. PHO87.

Phylogenomic databases

eggNOGfuNOG07970.
GeneTreeEFGT00050000003787.
HOGENOMHBG329859.
OMALLMAMSV.
OrthoDBEOG437VP2.

Gene expression databases

ArrayExpressP25360.
GenevestigatorP25360.
GermOnlineYCR037C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004680. Divalent_ion_symporter.
IPR004331. SPX_N.
[Graphical view]
KOK14430.
PfamPF03600. CitMHS. 1 hit.
PF03105. SPX. 1 hit.
[Graphical view]
PROSITEPS51382. SPX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965944.

Entry information

Entry namePHO87_YEAST
AccessionPrimary (citable) accession number: P25360
Secondary accession number(s): D6VR47
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: March 15, 2004
Last modified: December 14, 2011
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents