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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase 1

Gene

NPP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates extracellular nucleotide derived phosphate hydrolysis along with NPP2 and PHO5.1 Publication

Catalytic activityi

Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.Curated
A nucleoside triphosphate + H2O = a nucleotide + diphosphate.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei219NucleophileBy similarity1

GO - Molecular functioni

  • NADH pyrophosphatase activity Source: UniProtKB-EC
  • nucleoside-triphosphatase activity Source: SGD
  • nucleoside-triphosphate diphosphatase activity Source: SGD
  • nucleotide diphosphatase activity Source: SGD
  • phosphodiesterase I activity Source: SGD

GO - Biological processi

  • cellular response to phosphate starvation Source: SGD
  • nucleoside triphosphate metabolic process Source: SGD
  • phosphate-containing compound metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-29341-MONOMER.
ReactomeiR-SCE-1660662. Glycosphingolipid metabolism.
R-SCE-196843. Vitamin B2 (riboflavin) metabolism.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-6814848. Glycerophospholipid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase 1
Short name:
E-NPP 1
Including the following 2 domains:
Alkaline phosphodiesterase 1 (EC:3.1.4.1Curated)
Nucleotide pyrophosphatase (EC:3.6.1.9Curated)
Short name:
NPPase
Alternative name(s):
Nucleotide diphosphataseCurated
Gene namesi
Name:NPP1
Ordered Locus Names:YCR026C
ORF Names:YCR246, YCR26C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR026C.
SGDiS000000621. NPP1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 113CytoplasmicSequence analysisAdd BLAST113
Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
Topological domaini135 – 742ExtracellularSequence analysisAdd BLAST608

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002025661 – 742Ectonucleotide pyrophosphatase/phosphodiesterase 1Add BLAST742

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Glycosylationi204N-linked (GlcNAc...)Sequence analysis1
Glycosylationi264N-linked (GlcNAc...)Sequence analysis1
Glycosylationi296N-linked (GlcNAc...)Sequence analysis1
Glycosylationi403N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.
N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP25353.
PRIDEiP25353.

PTM databases

iPTMnetiP25353.

Expressioni

Inductioni

Up-regulated during phosphate starvation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi31008. 18 interactors.

Structurei

3D structure databases

ProteinModelPortaliP25353.
SMRiP25353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 545PhosphodiesteraseAdd BLAST378

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi692 – 702Poly-SerAdd BLAST11

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119157.
InParanoidiP25353.
OMAiETFPNHW.
OrthoDBiEOG092C1GZ6.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 3 hits.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

P25353-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELQNDLESL DNELNDFSED PFRDDFITDE DAVRSGWRSA WTRMKYWFYK
60 70 80 90 100
NRLKWTNNPI VIGDAKDSRD GSNFRRGIPL YELDANGQPI DTELVDENEL
110 120 130 140 150
SFGTGFHSKV PFKIIFRTLF GSLVFAIFLI LMINIAKPHH STRVLSHFGS
160 170 180 190 200
PEFDPYVKYF NGTHEFFPLT IVISLDGFHP SLISKRNTPF LHDLYELKYD
210 220 230 240 250
GGMNITSTPF MVPSFPTETF PNHWTLVTGQ YPIHHGIVSN VFWDPDLNEE
260 270 280 290 300
FHPGVLDPRI WNNNDTEPIW QTVQSAFDGD IPFKAATHMW PGSDVNYTKY
310 320 330 340 350
NEEKLQPEHK NPIARERTPF YFDEFNAKEP LSQKLSKIIE YVDMSTLNER
360 370 380 390 400
PQLILGYVPN VDAFGHKHGY PSESEYYYED FTETLGEVDT FLKQLVESLQ
410 420 430 440 450
ERNLTSFTNL VIVSDHGMSD IVVPSNVIIW EDLLDEKLRK DYVSHAYLEG
460 470 480 490 500
PMMAISLKDS GNINEVYHNL KTSIDEDKYT VYVNGNFPKE WNFNDGKNHH
510 520 530 540 550
MASIWIVPEP GYAVMKKEQL KKVAKGDHKD KNEDNVFTIG SHGYDNNAID
560 570 580 590 600
MRSVFIGMGP YFPQGYIEPF QNTEIYNLLC DICGVAEKDR NSNDGTGMLM
610 620 630 640 650
NQLREPQSSE EVEIEDDFDY LVSKFGEFST YNIIWGGYPE ETEQDNVDND
660 670 680 690 700
NDDNDDGNTD EIAAMPSSSL TIKLEMTTSI PSATETLLGE TSPSSRSSSS
710 720 730 740
SSIQASATAS TVGDWLQDII NDAKDLIDDI IDSIDDLVDS DT
Length:742
Mass (Da):84,734
Last modified:June 27, 2003 - v2
Checksum:i83BD5F00D69B09C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42978.1.
BK006937 Genomic DNA. Translation: DAA07505.1.
PIRiS19437.
S27380.
RefSeqiNP_009955.2. NM_001178741.1.

Genome annotation databases

EnsemblFungiiCAC42978; CAC42978; CAC42978.
YCR026C; YCR026C; YCR026C.
GeneIDi850391.
KEGGisce:YCR026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42978.1.
BK006937 Genomic DNA. Translation: DAA07505.1.
PIRiS19437.
S27380.
RefSeqiNP_009955.2. NM_001178741.1.

3D structure databases

ProteinModelPortaliP25353.
SMRiP25353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31008. 18 interactors.

PTM databases

iPTMnetiP25353.

Proteomic databases

MaxQBiP25353.
PRIDEiP25353.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAC42978; CAC42978; CAC42978.
YCR026C; YCR026C; YCR026C.
GeneIDi850391.
KEGGisce:YCR026C.

Organism-specific databases

EuPathDBiFungiDB:YCR026C.
SGDiS000000621. NPP1.

Phylogenomic databases

GeneTreeiENSGT00760000119157.
InParanoidiP25353.
OMAiETFPNHW.
OrthoDBiEOG092C1GZ6.

Enzyme and pathway databases

BioCyciYEAST:G3O-29341-MONOMER.
ReactomeiR-SCE-1660662. Glycosphingolipid metabolism.
R-SCE-196843. Vitamin B2 (riboflavin) metabolism.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-6814848. Glycerophospholipid catabolism.

Miscellaneous databases

PROiP25353.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 3 hits.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNPP1_YEAST
AccessioniPrimary (citable) accession number: P25353
Secondary accession number(s): D6VR36, Q8NIL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 27, 2003
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3420 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.