Reviewed,
UniProtKB/Swiss-Prot P25353 (NPP1_YEAST)
Last modified
June 16, 2009.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ectonucleotide pyrophosphatase/phosphodiesterase 1 Short name=E-NPP 1 Including the following 2 domains: 1- Recommended name: Alkaline phosphodiesterase 1 EC=3.1.4.1 2- Recommended name: Nucleotide pyrophosphatase Short name=NPPase EC=3.6.1.9 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 742 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Mediates extracellular nucleotide derived phosphate hydrolysis along with NPP2 and PHO5. Ref.5 |
| Catalytic activity | Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides. A dinucleotide + H2O = 2 mononucleotides. |
| Subcellular location | Membrane; Single-pass type II membrane protein Potential. |
| Induction | Up-regulated during phosphate starvation. Ref.5 |
| Post-translational modification | Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity. Ref.5 Ref.6 |
| Miscellaneous | Present with 3420 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Domain | Signal-anchor Transmembrane |
| Molecular function | Hydrolase |
| PTM | Glycoprotein Phosphoprotein |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | cellular response to phosphate starvation Ref.5 Inferred from expression pattern. Source: SGD phosphate metabolic process Ref.5Inferred from mutant phenotype. Source: SGD |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | nucleoside-triphosphatase activity Ref.5 Inferred from genetic interaction. Source: SGD nucleoside-triphosphate diphosphatase activity Ref.5Inferred from direct assay. Source: SGD nucleotide diphosphatase activityInferred from electronic annotation. Source: EC phosphodiesterase I activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 742 | 742 | Ectonucleotide pyrophosphatase/phosphodiesterase 1 | PRO_0000202566 | |||||
Regions | |||||||||
| Topological domain | 1 – 113 | 113 | Cytoplasmic Potential | ||||||
| Transmembrane | 114 – 134 | 21 | Potential | ||||||
| Topological domain | 135 – 742 | 608 | Extracellular Potential | ||||||
| Region | 168 – 545 | 378 | Phosphodiesterase | ||||||
| Compositional bias | 692 – 702 | 11 | Poly-Ser | ||||||
Sites | |||||||||
| Active site | 219 | 1 | By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 9 | 1 | Phosphoserine Ref.6 | ||||||
| Glycosylation | 161 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 204 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 264 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 296 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 403 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. Sgouros J.G.Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | Valles G., Volckaerts G. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. |
| [3] | "The complete sequence of K3B, a 7.9 kb fragment between PGK1 and CRY1 on chromosome III, reveals the presence of seven open reading frames." Bolle P.-A., Gilliquet V., Berben G., Dumont J., Hilger F. Yeast 8:205-213(1992) [PubMed: 1574926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 244-742. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [5] | "Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae." Kennedy E.J., Pillus L., Ghosh G. Eukaryot. Cell 4:1892-1901(2005) [PubMed: 16278456] [Abstract] Cited for: FUNCTION, NPP ACTIVITY, INDUCTION, AUTOPHOSPHORYLATION. |
| [6] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X59720 Genomic DNA. Translation: CAC42978.1. | |
| PIR | S19437. S27380. |
| RefSeq | NP_009955.2. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | YCR026C. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 850391. |
| GenomeReviews | Gene locus YCR026C in contig X59720_GR. |
| KEGG | sce:YCR026C. |
| NMPDR | fig|4932.3.peg.680. |
Organism-specific databases | |
| CYGD | YCR026c. |
| SGD | S000000621. NPP1. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P25353. |
| OMA | P25353. GNFPKEW. |
Enzyme and pathway databases | |
| BRENDA | 3.1.4.1. 250. 3.6.1.9. 250. |
Gene expression databases | |
| ArrayExpress | P25353. |
| GermOnline | YCR026C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR017849. Alkaline_Pase-like_a/b/a. IPR002591. Phosphodiest/P_Trfase. [Graphical view] |
| Gene3D | G3DSA:3.40.720.10. Alk_phosphtse. 1 hit. |
| Pfam | PF01663. Phosphodiest. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 965909. |
Entry information
| Entry name | NPP1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P25353 Secondary accession number(s): Q8NIL9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome III Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names |

Clusters with


