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P25353

- NPP1_YEAST

UniProt

P25353 - NPP1_YEAST

Protein

Ectonucleotide pyrophosphatase/phosphodiesterase 1

Gene

NPP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (27 Jun 2003)
      Previous versions | rss
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    Functioni

    Mediates extracellular nucleotide derived phosphate hydrolysis along with NPP2 and PHO5.1 Publication

    Catalytic activityi

    Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.
    A dinucleotide + H2O = 2 mononucleotides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei219 – 2191NucleophileBy similarity

    GO - Molecular functioni

    1. NADH pyrophosphatase activity Source: UniProtKB-EC
    2. nucleoside-triphosphatase activity Source: SGD
    3. nucleoside-triphosphate diphosphatase activity Source: SGD
    4. nucleotide diphosphatase activity Source: SGD
    5. phosphodiesterase I activity Source: SGD

    GO - Biological processi

    1. cellular response to phosphate starvation Source: SGD
    2. nucleic acid phosphodiester bond hydrolysis Source: GOC
    3. nucleoside triphosphate metabolic process Source: SGD
    4. phosphate-containing compound metabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29341-MONOMER.
    ReactomeiREACT_188975. Glycosphingolipid metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ectonucleotide pyrophosphatase/phosphodiesterase 1
    Short name:
    E-NPP 1
    Including the following 2 domains:
    Alkaline phosphodiesterase 1 (EC:3.1.4.1)
    Nucleotide pyrophosphatase (EC:3.6.1.9)
    Short name:
    NPPase
    Gene namesi
    Name:NPP1
    Ordered Locus Names:YCR026C
    ORF Names:YCR246, YCR26C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR026c.
    SGDiS000000621. NPP1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 742742Ectonucleotide pyrophosphatase/phosphodiesterase 1PRO_0000202566Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.
    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP25353.
    PaxDbiP25353.

    Expressioni

    Inductioni

    Up-regulated during phosphate starvation.1 Publication

    Gene expression databases

    GenevestigatoriP25353.

    Interactioni

    Protein-protein interaction databases

    BioGridi31008. 19 interactions.
    STRINGi4932.YCR026C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25353.
    SMRiP25353. Positions 168-606.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 113113CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini135 – 742608ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei114 – 13421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni168 – 545378PhosphodiesteraseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi692 – 70211Poly-SerAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1524.
    GeneTreeiENSGT00660000095322.
    OMAiGNFPKEW.
    OrthoDBiEOG74J9HK.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR024873. E-NPP.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view]
    PANTHERiPTHR10151. PTHR10151. 1 hit.
    PfamiPF01663. Phosphodiest. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P25353-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELQNDLESL DNELNDFSED PFRDDFITDE DAVRSGWRSA WTRMKYWFYK    50
    NRLKWTNNPI VIGDAKDSRD GSNFRRGIPL YELDANGQPI DTELVDENEL 100
    SFGTGFHSKV PFKIIFRTLF GSLVFAIFLI LMINIAKPHH STRVLSHFGS 150
    PEFDPYVKYF NGTHEFFPLT IVISLDGFHP SLISKRNTPF LHDLYELKYD 200
    GGMNITSTPF MVPSFPTETF PNHWTLVTGQ YPIHHGIVSN VFWDPDLNEE 250
    FHPGVLDPRI WNNNDTEPIW QTVQSAFDGD IPFKAATHMW PGSDVNYTKY 300
    NEEKLQPEHK NPIARERTPF YFDEFNAKEP LSQKLSKIIE YVDMSTLNER 350
    PQLILGYVPN VDAFGHKHGY PSESEYYYED FTETLGEVDT FLKQLVESLQ 400
    ERNLTSFTNL VIVSDHGMSD IVVPSNVIIW EDLLDEKLRK DYVSHAYLEG 450
    PMMAISLKDS GNINEVYHNL KTSIDEDKYT VYVNGNFPKE WNFNDGKNHH 500
    MASIWIVPEP GYAVMKKEQL KKVAKGDHKD KNEDNVFTIG SHGYDNNAID 550
    MRSVFIGMGP YFPQGYIEPF QNTEIYNLLC DICGVAEKDR NSNDGTGMLM 600
    NQLREPQSSE EVEIEDDFDY LVSKFGEFST YNIIWGGYPE ETEQDNVDND 650
    NDDNDDGNTD EIAAMPSSSL TIKLEMTTSI PSATETLLGE TSPSSRSSSS 700
    SSIQASATAS TVGDWLQDII NDAKDLIDDI IDSIDDLVDS DT 742
    Length:742
    Mass (Da):84,734
    Last modified:June 27, 2003 - v2
    Checksum:i83BD5F00D69B09C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42978.1.
    BK006937 Genomic DNA. Translation: DAA07505.1.
    PIRiS19437.
    S27380.
    RefSeqiNP_009955.2. NM_001178741.1.

    Genome annotation databases

    EnsemblFungiiYCR026C; YCR026C; YCR026C.
    GeneIDi850391.
    KEGGisce:YCR026C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42978.1 .
    BK006937 Genomic DNA. Translation: DAA07505.1 .
    PIRi S19437.
    S27380.
    RefSeqi NP_009955.2. NM_001178741.1.

    3D structure databases

    ProteinModelPortali P25353.
    SMRi P25353. Positions 168-606.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31008. 19 interactions.
    STRINGi 4932.YCR026C.

    Proteomic databases

    MaxQBi P25353.
    PaxDbi P25353.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR026C ; YCR026C ; YCR026C .
    GeneIDi 850391.
    KEGGi sce:YCR026C.

    Organism-specific databases

    CYGDi YCR026c.
    SGDi S000000621. NPP1.

    Phylogenomic databases

    eggNOGi COG1524.
    GeneTreei ENSGT00660000095322.
    OMAi GNFPKEW.
    OrthoDBi EOG74J9HK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29341-MONOMER.
    Reactomei REACT_188975. Glycosphingolipid metabolism.

    Miscellaneous databases

    NextBioi 965909.

    Gene expression databases

    Genevestigatori P25353.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR024873. E-NPP.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view ]
    PANTHERi PTHR10151. PTHR10151. 1 hit.
    Pfami PF01663. Phosphodiest. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The complete sequence of K3B, a 7.9 kb fragment between PGK1 and CRY1 on chromosome III, reveals the presence of seven open reading frames."
      Bolle P.-A., Gilliquet V., Berben G., Dumont J., Hilger F.
      Yeast 8:205-213(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 244-742.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae."
      Kennedy E.J., Pillus L., Ghosh G.
      Eukaryot. Cell 4:1892-1901(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, NPP ACTIVITY, INDUCTION, AUTOPHOSPHORYLATION.
    7. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
      Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
      Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNPP1_YEAST
    AccessioniPrimary (citable) accession number: P25353
    Secondary accession number(s): D6VR36, Q8NIL9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: June 27, 2003
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3420 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3