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Protein

54S ribosomal protein L32, mitochondrial

Gene

MRPL32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.2 Publications

Miscellaneous

Present with 1700 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29322-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L32, mitochondrial
Alternative name(s):
Mitochondrial large ribosomal subunit protein bL32m1 Publication
YmL32
Gene namesi
Name:MRPL32
Ordered Locus Names:YCR003W
ORF Names:YCR041, YCR3W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR003W
SGDiS000000596 MRPL32

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 71Mitochondrion1 PublicationAdd BLAST71
ChainiPRO_000003051872 – 18354S ribosomal protein L32, mitochondrialAdd BLAST112

Proteomic databases

MaxQBiP25348
PaxDbiP25348
PRIDEiP25348

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins. bL32m has a zinc binding site.2 Publications

Protein-protein interaction databases

BioGridi30981, 176 interactors
DIPiDIP-4987N
IntActiP25348, 3 interactors
MINTiP25348
STRINGi4932.YCR003W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20W1-183[»]
5MRCelectron microscopy3.25W72-183[»]
5MREelectron microscopy3.75W72-183[»]
5MRFelectron microscopy4.97W72-183[»]
ProteinModelPortaliP25348
SMRiP25348
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000248832
InParanoidiP25348
KOiK02911
OMAiCGHYKRA
OrthoDBiEOG092C5O76

Family and domain databases

InterProiView protein in InterPro
IPR002677 Ribosomal_L32p
IPR011332 Ribosomal_zn-bd
PfamiView protein in Pfam
PF01783 Ribosomal_L32p, 1 hit
SUPFAMiSSF57829 SSF57829, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSLIFGKQL AFHKIVPTTA IGWLVPLGNP SLQIPGQKQL GSIHRWLREK
60 70 80 90 100
LQQDHKDTED KDFFSNNGIL LAVPKKKVSH QKKRQKLYGP GKKQLKMIHH
110 120 130 140 150
LNKCPSCGHY KRANTLCMYC VGQISHIWKT HTAKEEIKPR QEEELSELDQ
160 170 180
RVLYPGRRDT KYTKDLKDKD NYLERRVRTL KKD
Length:183
Mass (Da):21,437
Last modified:May 1, 1992 - v1
Checksum:iC0F0D87C23AA2DEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S48552 Genomic DNA Translation: AAD13857.1
Z11113 Genomic DNA Translation: CAA77444.1
X59720 Genomic DNA Translation: CAA42340.1
AY693214 Genomic DNA Translation: AAT93233.1
BK006937 Genomic DNA Translation: DAA07482.1
PIRiS22265 R5BY32
RefSeqiNP_009929.1, NM_001178716.1

Genome annotation databases

EnsemblFungiiCAA42340; CAA42340; CAA42340
YCR003W; YCR003W; YCR003W
GeneIDi850359
KEGGisce:YCR003W

Similar proteinsi

Entry informationi

Entry nameiRM32_YEAST
AccessioniPrimary (citable) accession number: P25348
Secondary accession number(s): D6VR13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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