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Protein

54S ribosomal protein L32, mitochondrial

Gene

MRPL32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29322-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L32, mitochondrial
Alternative name(s):
YmL32
Gene namesi
Name:MRPL32
Ordered Locus Names:YCR003W
ORF Names:YCR041, YCR3W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR003w.
SGDiS000000596. MRPL32.

Subcellular locationi

Mitochondrion 2 Publications

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7171Mitochondrion1 PublicationAdd
BLAST
Chaini72 – 18311254S ribosomal protein L32, mitochondrialPRO_0000030518Add
BLAST

Proteomic databases

MaxQBiP25348.
PaxDbiP25348.

Expressioni

Gene expression databases

GenevestigatoriP25348.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).1 Publication

Protein-protein interaction databases

BioGridi30981. 8 interactions.
DIPiDIP-4987N.
IntActiP25348. 1 interaction.
MINTiMINT-512003.
STRINGi4932.YCR003W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20W1-183[»]
ProteinModelPortaliP25348.
SMRiP25348. Positions 72-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L32P family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0333.
HOGENOMiHOG000248832.
InParanoidiP25348.
KOiK02911.
OMAiKKKVSHQ.
OrthoDBiEOG780S0R.

Family and domain databases

InterProiIPR002677. Ribosomal_L32p.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PfamiPF01783. Ribosomal_L32p. 1 hit.
[Graphical view]
SUPFAMiSSF57829. SSF57829. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25348-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSLIFGKQL AFHKIVPTTA IGWLVPLGNP SLQIPGQKQL GSIHRWLREK
60 70 80 90 100
LQQDHKDTED KDFFSNNGIL LAVPKKKVSH QKKRQKLYGP GKKQLKMIHH
110 120 130 140 150
LNKCPSCGHY KRANTLCMYC VGQISHIWKT HTAKEEIKPR QEEELSELDQ
160 170 180
RVLYPGRRDT KYTKDLKDKD NYLERRVRTL KKD
Length:183
Mass (Da):21,437
Last modified:May 1, 1992 - v1
Checksum:iC0F0D87C23AA2DEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S48552 Genomic DNA. Translation: AAD13857.1.
Z11113 Genomic DNA. Translation: CAA77444.1.
X59720 Genomic DNA. Translation: CAA42340.1.
AY693214 Genomic DNA. Translation: AAT93233.1.
BK006937 Genomic DNA. Translation: DAA07482.1.
PIRiS22265. R5BY32.
RefSeqiNP_009929.1. NM_001178716.1.

Genome annotation databases

EnsemblFungiiYCR003W; YCR003W; YCR003W.
GeneIDi850359.
KEGGisce:YCR003W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S48552 Genomic DNA. Translation: AAD13857.1.
Z11113 Genomic DNA. Translation: CAA77444.1.
X59720 Genomic DNA. Translation: CAA42340.1.
AY693214 Genomic DNA. Translation: AAT93233.1.
BK006937 Genomic DNA. Translation: DAA07482.1.
PIRiS22265. R5BY32.
RefSeqiNP_009929.1. NM_001178716.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20W1-183[»]
ProteinModelPortaliP25348.
SMRiP25348. Positions 72-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30981. 8 interactions.
DIPiDIP-4987N.
IntActiP25348. 1 interaction.
MINTiMINT-512003.
STRINGi4932.YCR003W.

Proteomic databases

MaxQBiP25348.
PaxDbiP25348.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYCR003W; YCR003W; YCR003W.
GeneIDi850359.
KEGGisce:YCR003W.

Organism-specific databases

CYGDiYCR003w.
SGDiS000000596. MRPL32.

Phylogenomic databases

eggNOGiCOG0333.
HOGENOMiHOG000248832.
InParanoidiP25348.
KOiK02911.
OMAiKKKVSHQ.
OrthoDBiEOG780S0R.

Enzyme and pathway databases

BioCyciYEAST:G3O-29322-MONOMER.

Miscellaneous databases

NextBioi965833.

Gene expression databases

GenevestigatoriP25348.

Family and domain databases

InterProiIPR002677. Ribosomal_L32p.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PfamiPF01783. Ribosomal_L32p. 1 hit.
[Graphical view]
SUPFAMiSSF57829. SSF57829. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the CDC10 region at chromosome III of Saccharomyces cerevisiae."
    Steensma H.Y., van der Aart Q.J.M.
    Yeast 7:425-429(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete sequence of a 10.8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae."
    Biteau N., Fremaux C., Hebrard S., Menara A., Aigle M., Crouzet M.
    Yeast 8:61-70(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
    Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
    FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 72-106, SUBUNIT.
    Strain: 07173.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiRM32_YEAST
AccessioniPrimary (citable) accession number: P25348
Secondary accession number(s): D6VR13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 4, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.