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P25348

- RM32_YEAST

UniProt

P25348 - RM32_YEAST

Protein

54S ribosomal protein L32, mitochondrial

Gene

MRPL32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29322-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    54S ribosomal protein L32, mitochondrial
    Alternative name(s):
    YmL32
    Gene namesi
    Name:MRPL32
    Ordered Locus Names:YCR003W
    ORF Names:YCR041, YCR3W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR003w.
    SGDiS000000596. MRPL32.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB
    2. mitochondrial large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7171Mitochondrion1 PublicationAdd
    BLAST
    Chaini72 – 18311254S ribosomal protein L32, mitochondrialPRO_0000030518Add
    BLAST

    Proteomic databases

    MaxQBiP25348.
    PaxDbiP25348.

    Expressioni

    Gene expression databases

    GenevestigatoriP25348.

    Interactioni

    Subunit structurei

    Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).1 Publication

    Protein-protein interaction databases

    BioGridi30981. 8 interactions.
    DIPiDIP-4987N.
    IntActiP25348. 1 interaction.
    MINTiMINT-512003.
    STRINGi4932.YCR003W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW4electron microscopy3.20W1-183[»]
    ProteinModelPortaliP25348.
    SMRiP25348. Positions 72-121.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L32P family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0333.
    HOGENOMiHOG000248832.
    KOiK02911.
    OMAiKKKVSHQ.
    OrthoDBiEOG780S0R.

    Family and domain databases

    InterProiIPR002677. Ribosomal_L32p.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view]
    PfamiPF01783. Ribosomal_L32p. 1 hit.
    [Graphical view]
    SUPFAMiSSF57829. SSF57829. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25348-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSLIFGKQL AFHKIVPTTA IGWLVPLGNP SLQIPGQKQL GSIHRWLREK    50
    LQQDHKDTED KDFFSNNGIL LAVPKKKVSH QKKRQKLYGP GKKQLKMIHH 100
    LNKCPSCGHY KRANTLCMYC VGQISHIWKT HTAKEEIKPR QEEELSELDQ 150
    RVLYPGRRDT KYTKDLKDKD NYLERRVRTL KKD 183
    Length:183
    Mass (Da):21,437
    Last modified:May 1, 1992 - v1
    Checksum:iC0F0D87C23AA2DEA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S48552 Genomic DNA. Translation: AAD13857.1.
    Z11113 Genomic DNA. Translation: CAA77444.1.
    X59720 Genomic DNA. Translation: CAA42340.1.
    AY693214 Genomic DNA. Translation: AAT93233.1.
    BK006937 Genomic DNA. Translation: DAA07482.1.
    PIRiS22265. R5BY32.
    RefSeqiNP_009929.1. NM_001178716.1.

    Genome annotation databases

    EnsemblFungiiYCR003W; YCR003W; YCR003W.
    GeneIDi850359.
    KEGGisce:YCR003W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S48552 Genomic DNA. Translation: AAD13857.1 .
    Z11113 Genomic DNA. Translation: CAA77444.1 .
    X59720 Genomic DNA. Translation: CAA42340.1 .
    AY693214 Genomic DNA. Translation: AAT93233.1 .
    BK006937 Genomic DNA. Translation: DAA07482.1 .
    PIRi S22265. R5BY32.
    RefSeqi NP_009929.1. NM_001178716.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW4 electron microscopy 3.20 W 1-183 [» ]
    ProteinModelPortali P25348.
    SMRi P25348. Positions 72-121.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30981. 8 interactions.
    DIPi DIP-4987N.
    IntActi P25348. 1 interaction.
    MINTi MINT-512003.
    STRINGi 4932.YCR003W.

    Proteomic databases

    MaxQBi P25348.
    PaxDbi P25348.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR003W ; YCR003W ; YCR003W .
    GeneIDi 850359.
    KEGGi sce:YCR003W.

    Organism-specific databases

    CYGDi YCR003w.
    SGDi S000000596. MRPL32.

    Phylogenomic databases

    eggNOGi COG0333.
    HOGENOMi HOG000248832.
    KOi K02911.
    OMAi KKKVSHQ.
    OrthoDBi EOG780S0R.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29322-MONOMER.

    Miscellaneous databases

    NextBioi 965833.

    Gene expression databases

    Genevestigatori P25348.

    Family and domain databases

    InterProi IPR002677. Ribosomal_L32p.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view ]
    Pfami PF01783. Ribosomal_L32p. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57829. SSF57829. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the CDC10 region at chromosome III of Saccharomyces cerevisiae."
      Steensma H.Y., van der Aart Q.J.M.
      Yeast 7:425-429(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete sequence of a 10.8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae."
      Biteau N., Fremaux C., Hebrard S., Menara A., Aigle M., Crouzet M.
      Yeast 8:61-70(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
      Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
      FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 72-106, SUBUNIT.
      Strain: 07173.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiRM32_YEAST
    AccessioniPrimary (citable) accession number: P25348
    Secondary accession number(s): D6VR13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3