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P25345

- SYNM_YEAST

UniProt

P25345 - SYNM_YEAST

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Protein

Asparagine--tRNA ligase, mitochondrial

Gene

SLM5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).

GO - Molecular functioni

  1. asparagine-tRNA ligase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. asparaginyl-tRNA aminoacylation Source: UniProtKB
  2. mitochondrial asparaginyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29339-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligase, mitochondrial (EC:6.1.1.22)
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name:
AsnRS
Gene namesi
Name:SLM5
Ordered Locus Names:YCR024C
ORF Names:YCR242, YCR24C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR024c.
SGDiS000000618. SLM5.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 492Asparagine--tRNA ligase, mitochondrialPRO_0000035801
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

MaxQBiP25345.
PaxDbiP25345.

Expressioni

Gene expression databases

GenevestigatoriP25345.

Interactioni

Protein-protein interaction databases

BioGridi31006. 23 interactions.
DIPiDIP-2597N.
IntActiP25345. 4 interactions.
MINTiMINT-426906.
STRINGi4932.YCR024C.

Structurei

3D structure databases

ProteinModelPortaliP25345.
SMRiP25345. Positions 34-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0017.
GeneTreeiENSGT00550000074970.
HOGENOMiHOG000226033.
InParanoidiP25345.
KOiK01893.
OMAiHLRIRTP.
OrthoDBiEOG7K0ZMR.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF6. PTHR22594:SF6. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25345-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFHAFTFLKG GRFYSSLTVK SLYEQVHHTS HDPISINGWI KSIRLLKRIA
60 70 80 90 100
FLDLQDGTSV NPLRIVIPLT NTDEVQFLKI LKTGQTLSIS NATWQSTPNR
110 120 130 140 150
KQPFELQIKN PVKSIKLVGP VSENYPLQKK YQTLRYLRSL PTLKYRTAYL
160 170 180 190 200
SAILRLRSFV EFQFMLYFQK NHFTKVSPPI LTSNDCEGAG ELFQVSTNTS
210 220 230 240 250
PTASSYFGKP TYLTVSTQLH LEILALSLSR CWTLSPCFRA EKSDTPRHLS
260 270 280 290 300
EFWMLEVEMC FVNSVNELTS FVETTIKHII KACIDNQQEL LPKQFISSQE
310 320 330 340 350
NNASSELSIN QETQQIKTRW EDLINEKWHN ITYTNAIEIL KKRHNEVSHF
360 370 380 390 400
KYEPKWGQPL QTEHEKFLAG EYFKSPVFVT DYPRLCKPFY MKQNSTPDDT
410 420 430 440 450
VGCFDLLVPG MGEIIGGSLR EDDYDKLCRE MKARGMNRSG ELDWYVSLRK
460 470 480 490
EGSAPHGGFG LGFERFISYL YGNHNIKDAI PFYRTSAESI DF
Length:492
Mass (Da):56,785
Last modified:May 1, 1992 - v1
Checksum:iE20C6F921C9BAA9C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42316.1.
BK006937 Genomic DNA. Translation: DAA07502.1.
PIRiS19435.
RefSeqiNP_009953.1. NM_001178738.1.

Genome annotation databases

EnsemblFungiiYCR024C; YCR024C; YCR024C.
GeneIDi850388.
KEGGisce:YCR024C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42316.1 .
BK006937 Genomic DNA. Translation: DAA07502.1 .
PIRi S19435.
RefSeqi NP_009953.1. NM_001178738.1.

3D structure databases

ProteinModelPortali P25345.
SMRi P25345. Positions 34-487.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31006. 23 interactions.
DIPi DIP-2597N.
IntActi P25345. 4 interactions.
MINTi MINT-426906.
STRINGi 4932.YCR024C.

Proteomic databases

MaxQBi P25345.
PaxDbi P25345.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR024C ; YCR024C ; YCR024C .
GeneIDi 850388.
KEGGi sce:YCR024C.

Organism-specific databases

CYGDi YCR024c.
SGDi S000000618. SLM5.

Phylogenomic databases

eggNOGi COG0017.
GeneTreei ENSGT00550000074970.
HOGENOMi HOG000226033.
InParanoidi P25345.
KOi K01893.
OMAi HLRIRTP.
OrthoDBi EOG7K0ZMR.

Enzyme and pathway databases

BioCyci YEAST:G3O-29339-MONOMER.

Miscellaneous databases

NextBioi 965903.
PROi P25345.

Gene expression databases

Genevestigatori P25345.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
InterProi IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view ]
PANTHERi PTHR22594. PTHR22594. 1 hit.
PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfami PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
PRINTSi PR01042. TRNASYNTHASP.
SUPFAMi SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00457. asnS. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of K3B, a 7.9 kb fragment between PGK1 and CRY1 on chromosome III, reveals the presence of seven open reading frames."
    Bolle P.-A., Gilliquet V., Berben G., Dumont J., Hilger F.
    Yeast 8:205-213(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Mitochondrial asparaginyl-tRNA synthetase is encoded by the yeast nuclear gene YCR24c."
    Landrieu I., Vandenbol M., Hartlein M., Portetelle D.
    Eur. J. Biochem. 243:268-273(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYNM_YEAST
AccessioniPrimary (citable) accession number: P25345
Secondary accession number(s): D6VR33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3