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P25345

- SYNM_YEAST

UniProt

P25345 - SYNM_YEAST

Protein

Asparagine--tRNA ligase, mitochondrial

Gene

SLM5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).

    GO - Molecular functioni

    1. asparagine-tRNA ligase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. asparaginyl-tRNA aminoacylation Source: UniProtKB
    2. mitochondrial asparaginyl-tRNA aminoacylation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29339-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine--tRNA ligase, mitochondrial (EC:6.1.1.22)
    Alternative name(s):
    Asparaginyl-tRNA synthetase
    Short name:
    AsnRS
    Gene namesi
    Name:SLM5
    Ordered Locus Names:YCR024C
    ORF Names:YCR242, YCR24C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR024c.
    SGDiS000000618. SLM5.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 492Asparagine--tRNA ligase, mitochondrialPRO_0000035801
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Proteomic databases

    MaxQBiP25345.
    PaxDbiP25345.

    Expressioni

    Gene expression databases

    GenevestigatoriP25345.

    Interactioni

    Protein-protein interaction databases

    BioGridi31006. 23 interactions.
    DIPiDIP-2597N.
    IntActiP25345. 4 interactions.
    MINTiMINT-426906.
    STRINGi4932.YCR024C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25345.
    SMRiP25345. Positions 34-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0017.
    GeneTreeiENSGT00550000074970.
    HOGENOMiHOG000226033.
    KOiK01893.
    OMAiHLRIRTP.
    OrthoDBiEOG7K0ZMR.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004522. Asn-tRNA-ligase.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF6. PTHR22594:SF6. 1 hit.
    PfamiPF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00457. asnS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25345-1 [UniParc]FASTAAdd to Basket

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    MFHAFTFLKG GRFYSSLTVK SLYEQVHHTS HDPISINGWI KSIRLLKRIA    50
    FLDLQDGTSV NPLRIVIPLT NTDEVQFLKI LKTGQTLSIS NATWQSTPNR 100
    KQPFELQIKN PVKSIKLVGP VSENYPLQKK YQTLRYLRSL PTLKYRTAYL 150
    SAILRLRSFV EFQFMLYFQK NHFTKVSPPI LTSNDCEGAG ELFQVSTNTS 200
    PTASSYFGKP TYLTVSTQLH LEILALSLSR CWTLSPCFRA EKSDTPRHLS 250
    EFWMLEVEMC FVNSVNELTS FVETTIKHII KACIDNQQEL LPKQFISSQE 300
    NNASSELSIN QETQQIKTRW EDLINEKWHN ITYTNAIEIL KKRHNEVSHF 350
    KYEPKWGQPL QTEHEKFLAG EYFKSPVFVT DYPRLCKPFY MKQNSTPDDT 400
    VGCFDLLVPG MGEIIGGSLR EDDYDKLCRE MKARGMNRSG ELDWYVSLRK 450
    EGSAPHGGFG LGFERFISYL YGNHNIKDAI PFYRTSAESI DF 492
    Length:492
    Mass (Da):56,785
    Last modified:May 1, 1992 - v1
    Checksum:iE20C6F921C9BAA9C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42316.1.
    BK006937 Genomic DNA. Translation: DAA07502.1.
    PIRiS19435.
    RefSeqiNP_009953.1. NM_001178738.1.

    Genome annotation databases

    EnsemblFungiiYCR024C; YCR024C; YCR024C.
    GeneIDi850388.
    KEGGisce:YCR024C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42316.1 .
    BK006937 Genomic DNA. Translation: DAA07502.1 .
    PIRi S19435.
    RefSeqi NP_009953.1. NM_001178738.1.

    3D structure databases

    ProteinModelPortali P25345.
    SMRi P25345. Positions 34-487.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31006. 23 interactions.
    DIPi DIP-2597N.
    IntActi P25345. 4 interactions.
    MINTi MINT-426906.
    STRINGi 4932.YCR024C.

    Proteomic databases

    MaxQBi P25345.
    PaxDbi P25345.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR024C ; YCR024C ; YCR024C .
    GeneIDi 850388.
    KEGGi sce:YCR024C.

    Organism-specific databases

    CYGDi YCR024c.
    SGDi S000000618. SLM5.

    Phylogenomic databases

    eggNOGi COG0017.
    GeneTreei ENSGT00550000074970.
    HOGENOMi HOG000226033.
    KOi K01893.
    OMAi HLRIRTP.
    OrthoDBi EOG7K0ZMR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29339-MONOMER.

    Miscellaneous databases

    NextBioi 965903.
    PROi P25345.

    Gene expression databases

    Genevestigatori P25345.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004522. Asn-tRNA-ligase.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF6. PTHR22594:SF6. 1 hit.
    Pfami PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR01042. TRNASYNTHASP.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00457. asnS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of K3B, a 7.9 kb fragment between PGK1 and CRY1 on chromosome III, reveals the presence of seven open reading frames."
      Bolle P.-A., Gilliquet V., Berben G., Dumont J., Hilger F.
      Yeast 8:205-213(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Mitochondrial asparaginyl-tRNA synthetase is encoded by the yeast nuclear gene YCR24c."
      Landrieu I., Vandenbol M., Hartlein M., Portetelle D.
      Eur. J. Biochem. 243:268-273(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYNM_YEAST
    AccessioniPrimary (citable) accession number: P25345
    Secondary accession number(s): D6VR33
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 784 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3