Reviewed, UniProtKB/Swiss-Prot P25345 (SYNM_YEAST)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase, mitochondrial
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	SLM5
Ordered Locus Names:	YCR024C
ORF Names:	YCR24C, YCR242

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 784 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial asparaginyl-tRNA aminoacylation Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Ref.3 Inferred from sequence or structural similarity. Source: UniProtKB aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP40	P33203	1	EBI-18781,EBI-701

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 492		Asparaginyl-tRNA synthetase, mitochondrial		PRO_0000035801

Sequences

	Sequence		Length	Mass (Da)
	P25345-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: E20C6F921C9BAA9C	FASTA	492	56,785
	10 20 30 40 50 60 MFHAFTFLKG GRFYSSLTVK SLYEQVHHTS HDPISINGWI KSIRLLKRIA FLDLQDGTSV 70 80 90 100 110 120 NPLRIVIPLT NTDEVQFLKI LKTGQTLSIS NATWQSTPNR KQPFELQIKN PVKSIKLVGP 130 140 150 160 170 180 VSENYPLQKK YQTLRYLRSL PTLKYRTAYL SAILRLRSFV EFQFMLYFQK NHFTKVSPPI 190 200 210 220 230 240 LTSNDCEGAG ELFQVSTNTS PTASSYFGKP TYLTVSTQLH LEILALSLSR CWTLSPCFRA 250 260 270 280 290 300 EKSDTPRHLS EFWMLEVEMC FVNSVNELTS FVETTIKHII KACIDNQQEL LPKQFISSQE 310 320 330 340 350 360 NNASSELSIN QETQQIKTRW EDLINEKWHN ITYTNAIEIL KKRHNEVSHF KYEPKWGQPL 370 380 390 400 410 420 QTEHEKFLAG EYFKSPVFVT DYPRLCKPFY MKQNSTPDDT VGCFDLLVPG MGEIIGGSLR 430 440 450 460 470 480 EDDYDKLCRE MKARGMNRSG ELDWYVSLRK EGSAPHGGFG LGFERFISYL YGNHNIKDAI 490 PFYRTSAESI DF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of K3B, a 7.9 kb fragment between PGK1 and CRY1 on chromosome III, reveals the presence of seven open reading frames." Bolle P.-A., Gilliquet V., Berben G., Dumont J., Hilger F. Yeast 8:205-213(1992) [PubMed: 1574926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Mitochondrial asparaginyl-tRNA synthetase is encoded by the yeast nuclear gene YCR24c." Landrieu I., Vandenbol M., Hartlein M., Portetelle D. Eur. J. Biochem. 243:268-273(1997) [PubMed: 9030748] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59720 Genomic DNA. Translation: CAA42316.1.
PIR	S19435.
RefSeq	NP_009953.1.
3D structure databases
HSSP	HSSP built from PDB template 1N9W based on UniProtKB Q9LCY8.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2597N.
IntAct	P25345. 3 interactions.
STRING	P25345.
Genome annotation databases
Ensembl	YCR024C; YCR024C; YCR024C; Saccharomyces cerevisiae. [Genome view]
GeneID	850388.
GenomeReviews	Gene locus YCR024C in contig X59720_GR.
KEGG	sce:YCR024C.
NMPDR	fig|4932.3.peg.676.
Organism-specific databases
CYGD	YCR024c.
SGD	S000000618. SLM5.
Phylogenomic databases
HOGENOM	P25345.
OMA	VGELCGG.
Enzyme and pathway databases
BRENDA	6.1.1.22. 250.
Gene expression databases
ArrayExpress	P25345.
Genevestigator	P25345.
GermOnline	YCR024C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	965903.

Entry information

Entry name

SYNM_YEAST

Accession

Primary (citable) accession number: P25345

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	November 3, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)