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P25344 (STE50_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein STE50
Gene names
Name:STE50
Ordered Locus Names:YCL032W
ORF Names:YCL32W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in growth arrest during conjugation. May interact with the G protein alpha subunit.

Subunit structure

Interacts with STE11 through the respective SAM domains. Ref.9 Ref.10

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 Ras-associating domain.

Contains 1 SAM (sterile alpha motif) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SHO1P400733EBI-18305,EBI-18140
STE11P235616EBI-18305,EBI-18259

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Protein STE50
PRO_0000072265

Regions

Domain24 – 10885SAM
Domain233 – 32795Ras-associating

Amino acid modifications

Modified residue2021Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue2441Phosphothreonine Ref.8
Modified residue2481Phosphoserine Ref.6 Ref.8

Secondary structure

.............. 346
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25344 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: EFC7D14F2940C779

FASTA34638,971
        10         20         30         40         50         60 
MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV EETDPLCQRL 

        70         80         90        100        110        120 
RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR DSKLEWKDDK TQEDMITVLK 

       130        140        150        160        170        180 
NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT SSSSSPINTH GVSTTVPSSN NTIIPSSDGV 

       190        200        210        220        230        240 
SLSQTDYFDT VHNRQSPSRR ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS 

       250        260        270        280        290        300 
TANTPGPSPN EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL 

       310        320        330        340 
NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL 

« Hide

References

« Hide 'large scale' references
[1]"STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae."
Rad M.R., Xu G., Hollenberg C.P.
Mol. Gen. Genet. 236:145-154(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202; THR-244 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM."
Grimshaw S.J., Mott H.R., Stott K.M., Nielsen P.R., Evetts K.A., Hopkins L.J., Nietlispach D., Owen D.
J. Biol. Chem. 279:2192-2201(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 27-108, INTERACTION WITH STE11.
[10]"Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction."
Kwan J.J., Warner N., Maini J., Chan Tung K.W., Zakaria H., Pawson T., Donaldson L.W.
J. Mol. Biol. 356:142-154(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 32-107, INTERACTION WITH STE11.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11116 Genomic DNA. Translation: CAA77462.1.
S54312 Genomic DNA. Translation: AAA13629.1.
X59720 Genomic DNA. Translation: CAA42384.1.
BK006937 Genomic DNA. Translation: DAA07452.1.
PIRS17475.
RefSeqNP_009898.1. NM_001178677.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UQVNMR-A27-108[»]
1Z1VNMR-A32-107[»]
ProteinModelPortalP25344.
SMRP25344. Positions 27-108, 250-343.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid30951. 383 interactions.
DIPDIP-667N.
IntActP25344. 37 interactions.
MINTMINT-408633.
STRING4932.YCL032W.

Proteomic databases

PaxDbP25344.
PeptideAtlasP25344.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCL032W; YCL032W; YCL032W.
GeneID850325.
KEGGsce:YCL032W.

Organism-specific databases

CYGDYCL032w.
SGDS000000537. STE50.

Phylogenomic databases

eggNOGNOG46436.
HOGENOMHOG000057098.
OMAGLHPAIM.
OrthoDBEOG7GFBFJ.

Enzyme and pathway databases

BioCycYEAST:G3O-29292-MONOMER.

Gene expression databases

GenevestigatorP25344.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
InterProIPR000159. Ras-assoc.
IPR013761. SAM/pointed.
IPR015316. SAM_Ste50p.
[Graphical view]
PfamPF00788. RA. 1 hit.
PF09235. Ste50p-SAM. 1 hit.
[Graphical view]
SMARTSM00314. RA. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
PROSITEPS50200. RA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP25344.
NextBio965745.

Entry information

Entry nameSTE50_YEAST
AccessionPrimary (citable) accession number: P25344
Secondary accession number(s): D6VQY3, Q3S1N2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references