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Protein

Protein STE50

Gene

STE50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in growth arrest during conjugation. May interact with the G protein alpha subunit.

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein kinase regulator activity Source: SGD
  • SAM domain binding Source: SGD

GO - Biological processi

  • cell cycle arrest Source: UniProtKB-KW
  • cellular response to heat Source: SGD
  • MAPK cascade involved in osmosensory signaling pathway Source: SGD
  • osmosensory signaling pathway via Sho1 osmosensor Source: SGD
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  • signal transduction involved in filamentous growth Source: SGD

Keywordsi

Biological processCell cycle, Growth arrest, Pheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-29292-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein STE50
Gene namesi
Name:STE50
Ordered Locus Names:YCL032W
ORF Names:YCL32W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL032W.
SGDiS000000537. STE50.

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000722651 – 346Protein STE50Add BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei202PhosphoserineCombined sources1
Modified residuei244PhosphothreonineCombined sources1
Modified residuei248PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25344.
PRIDEiP25344.

PTM databases

iPTMnetiP25344.

Interactioni

Subunit structurei

Interacts with STE11 through the respective SAM domains.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • SAM domain binding Source: SGD

Protein-protein interaction databases

BioGridi30951. 723 interactors.
DIPiDIP-667N.
IntActiP25344. 51 interactors.
MINTiMINT-408633.
STRINGi4932.YCL032W.

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni32 – 34Combined sources3
Helixi37 – 48Combined sources12
Helixi56 – 62Combined sources7
Helixi67 – 69Combined sources3
Helixi70 – 80Combined sources11
Beta strandi82 – 84Combined sources3
Helixi86 – 101Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UQVNMR-A27-108[»]
1Z1VNMR-A32-107[»]
ProteinModelPortaliP25344.
SMRiP25344.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25344.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 108SAMAdd BLAST85
Domaini233 – 327Ras-associatingPROSITE-ProRule annotationAdd BLAST95

Phylogenomic databases

HOGENOMiHOG000057098.
InParanoidiP25344.
KOiK19841.
OMAiGLHPAIM.
OrthoDBiEOG092C3EN8.

Family and domain databases

CDDicd09536. SAM_Ste50_fungal. 1 hit.
InterProiView protein in InterPro
IPR000159. RA_dom.
IPR013761. SAM/pointed.
IPR015316. SAM_Ste50.
IPR029071. Ubiquitin-rel_dom.
PfamiView protein in Pfam
PF00788. RA. 1 hit.
PF09235. Ste50p-SAM. 1 hit.
SMARTiView protein in SMART
SM00314. RA. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50200. RA. 1 hit.

Sequencei

Sequence statusi: Complete.

P25344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV
60 70 80 90 100
EETDPLCQRL RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR
110 120 130 140 150
DSKLEWKDDK TQEDMITVLK NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT
160 170 180 190 200
SSSSSPINTH GVSTTVPSSN NTIIPSSDGV SLSQTDYFDT VHNRQSPSRR
210 220 230 240 250
ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS TANTPGPSPN
260 270 280 290 300
EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL
310 320 330 340
NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL
Length:346
Mass (Da):38,971
Last modified:May 1, 1992 - v1
Checksum:iEFC7D14F2940C779
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11116 Genomic DNA. Translation: CAA77462.1.
S54312 Genomic DNA. Translation: AAA13629.1.
X59720 Genomic DNA. Translation: CAA42384.1.
BK006937 Genomic DNA. Translation: DAA07452.1.
PIRiS17475.
RefSeqiNP_009898.1. NM_001178677.1.

Genome annotation databases

EnsemblFungiiCAA42384; CAA42384; CAA42384.
YCL032W; YCL032W; YCL032W.
GeneIDi850325.
KEGGisce:YCL032W.

Similar proteinsi

Entry informationi

Entry nameiSTE50_YEAST
AccessioniPrimary (citable) accession number: P25344
Secondary accession number(s): D6VQY3, Q3S1N2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 27, 2017
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names