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P25344

- STE50_YEAST

UniProt

P25344 - STE50_YEAST

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Protein

Protein STE50

Gene
STE50, YCL032W, YCL32W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in growth arrest during conjugation. May interact with the G protein alpha subunit.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein kinase regulator activity Source: SGD
  3. SAM domain binding Source: SGD

GO - Biological processi

  1. cell cycle arrest Source: UniProtKB-KW
  2. cellular response to heat Source: SGD
  3. MAPK cascade involved in osmosensory signaling pathway Source: SGD
  4. osmosensory signaling pathway via Sho1 osmosensor Source: SGD
  5. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  6. regulation of protein kinase activity Source: GOC
  7. signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Growth arrest, Pheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-29292-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein STE50
Gene namesi
Name:STE50
Ordered Locus Names:YCL032W
ORF Names:YCL32W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL032w.
SGDiS000000537. STE50.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Protein STE50PRO_0000072265Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei202 – 2021Phosphoserine3 Publications
Modified residuei244 – 2441Phosphothreonine1 Publication
Modified residuei248 – 2481Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25344.
PaxDbiP25344.
PeptideAtlasiP25344.

Expressioni

Gene expression databases

GenevestigatoriP25344.

Interactioni

Subunit structurei

Interacts with STE11 through the respective SAM domains.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SHO1P400733EBI-18305,EBI-18140
STE11P235616EBI-18305,EBI-18259

Protein-protein interaction databases

BioGridi30951. 383 interactions.
DIPiDIP-667N.
IntActiP25344. 37 interactions.
MINTiMINT-408633.
STRINGi4932.YCL032W.

Structurei

Secondary structure

1
346
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni32 – 343
Helixi37 – 4812
Helixi56 – 627
Helixi67 – 693
Helixi70 – 8011
Beta strandi82 – 843
Helixi86 – 10116

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UQVNMR-A27-108[»]
1Z1VNMR-A32-107[»]
ProteinModelPortaliP25344.
SMRiP25344. Positions 27-108.

Miscellaneous databases

EvolutionaryTraceiP25344.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 10885SAMAdd
BLAST
Domaini233 – 32795Ras-associatingAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG46436.
HOGENOMiHOG000057098.
OMAiGLHPAIM.
OrthoDBiEOG7GFBFJ.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR000159. Ras-assoc.
IPR013761. SAM/pointed.
IPR015316. SAM_Ste50p.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF09235. Ste50p-SAM. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25344-1 [UniParc]FASTAAdd to Basket

« Hide

MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV    50
EETDPLCQRL RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR 100
DSKLEWKDDK TQEDMITVLK NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT 150
SSSSSPINTH GVSTTVPSSN NTIIPSSDGV SLSQTDYFDT VHNRQSPSRR 200
ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS TANTPGPSPN 250
EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL 300
NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL 346
Length:346
Mass (Da):38,971
Last modified:May 1, 1992 - v1
Checksum:iEFC7D14F2940C779
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11116 Genomic DNA. Translation: CAA77462.1.
S54312 Genomic DNA. Translation: AAA13629.1.
X59720 Genomic DNA. Translation: CAA42384.1.
BK006937 Genomic DNA. Translation: DAA07452.1.
PIRiS17475.
RefSeqiNP_009898.1. NM_001178677.1.

Genome annotation databases

EnsemblFungiiYCL032W; YCL032W; YCL032W.
GeneIDi850325.
KEGGisce:YCL032W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11116 Genomic DNA. Translation: CAA77462.1 .
S54312 Genomic DNA. Translation: AAA13629.1 .
X59720 Genomic DNA. Translation: CAA42384.1 .
BK006937 Genomic DNA. Translation: DAA07452.1 .
PIRi S17475.
RefSeqi NP_009898.1. NM_001178677.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1UQV NMR - A 27-108 [» ]
1Z1V NMR - A 32-107 [» ]
ProteinModelPortali P25344.
SMRi P25344. Positions 27-108.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30951. 383 interactions.
DIPi DIP-667N.
IntActi P25344. 37 interactions.
MINTi MINT-408633.
STRINGi 4932.YCL032W.

Proteomic databases

MaxQBi P25344.
PaxDbi P25344.
PeptideAtlasi P25344.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL032W ; YCL032W ; YCL032W .
GeneIDi 850325.
KEGGi sce:YCL032W.

Organism-specific databases

CYGDi YCL032w.
SGDi S000000537. STE50.

Phylogenomic databases

eggNOGi NOG46436.
HOGENOMi HOG000057098.
OMAi GLHPAIM.
OrthoDBi EOG7GFBFJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-29292-MONOMER.

Miscellaneous databases

EvolutionaryTracei P25344.
NextBioi 965745.

Gene expression databases

Genevestigatori P25344.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
InterProi IPR000159. Ras-assoc.
IPR013761. SAM/pointed.
IPR015316. SAM_Ste50p.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF00788. RA. 1 hit.
PF09235. Ste50p-SAM. 1 hit.
[Graphical view ]
SMARTi SM00314. RA. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50200. RA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae."
    Rad M.R., Xu G., Hollenberg C.P.
    Mol. Gen. Genet. 236:145-154(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
    Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
    Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202; THR-244 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM."
    Grimshaw S.J., Mott H.R., Stott K.M., Nielsen P.R., Evetts K.A., Hopkins L.J., Nietlispach D., Owen D.
    J. Biol. Chem. 279:2192-2201(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 27-108, INTERACTION WITH STE11.
  10. "Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction."
    Kwan J.J., Warner N., Maini J., Chan Tung K.W., Zakaria H., Pawson T., Donaldson L.W.
    J. Mol. Biol. 356:142-154(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 32-107, INTERACTION WITH STE11.

Entry informationi

Entry nameiSTE50_YEAST
AccessioniPrimary (citable) accession number: P25344
Secondary accession number(s): D6VQY3, Q3S1N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

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