P25344 (STE50_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein STE50

Gene names

Name:	STE50
Ordered Locus Names:	YCL032W
ORF Names:	YCL32W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in growth arrest during conjugation. May interact with the G protein alpha subunit.
Subunit structure	Interacts with STE11 through the respective SAM domains. Ref.9 Ref.10
Miscellaneous	Present with 1670 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 Ras-associating domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Biological process	Cell cycle Growth arrest Pheromone response
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade involved in osmosensory signaling pathway Inferred from mutant phenotype PubMed 9742096. Source: SGD cell cycle arrest Inferred from electronic annotation. Source: UniProtKB-KW cellular response to heat Inferred from mutant phenotype PubMed 12455951. Source: SGD osmosensory signaling pathway via Sho1 osmosensor Inferred from mutant phenotype PubMed 11370856. Source: SGD pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from physical interaction PubMed 8793874. Source: SGD signal transduction involved in filamentous growth Inferred from physical interaction PubMed 9738877. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 9742096. Source: SGD
Molecular_function	SAM domain binding Inferred from direct assay Ref.10. Source: SGD protein kinase regulator activity Inferred from physical interaction PubMed 10397774. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STE11	P23561	6	EBI-18305,EBI-18259

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 346

346

Protein STE50

PRO_0000072265

Regions

Domain

24 – 108

85

SAM

Domain

233 – 327

95

Ras-associating

Amino acid modifications

Modified residue

202

1

Phosphoserine Ref.6 Ref.7 Ref.8

Modified residue

248

1

Phosphoserine Ref.6 Ref.8

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

346

Helix Strand Turn

Details...

Turn

32 – 34

3

Helix

37 – 48

12

Helix

56 – 62

7

Helix

67 – 69

3

Helix

70 – 80

11

Beta strand

82 – 84

3

Helix

86 – 101

16

Sequences

	Sequence		Length	Mass (Da)
	P25344 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: EFC7D14F2940C779	FASTA	346	38,971
	10 20 30 40 50 60 MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV EETDPLCQRL 70 80 90 100 110 120 RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR DSKLEWKDDK TQEDMITVLK 130 140 150 160 170 180 NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT SSSSSPINTH GVSTTVPSSN NTIIPSSDGV 190 200 210 220 230 240 SLSQTDYFDT VHNRQSPSRR ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS 250 260 270 280 290 300 TANTPGPSPN EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL 310 320 330 340 NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae." Rad M.R., Xu G., Hollenberg C.P. Mol. Gen. Genet. 236:145-154(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames." Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P. Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202 AND SER-248, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202 AND SER-248, MASS SPECTROMETRY.
[9]	"Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM." Grimshaw S.J., Mott H.R., Stott K.M., Nielsen P.R., Evetts K.A., Hopkins L.J., Nietlispach D., Owen D. J. Biol. Chem. 279:2192-2201(2004) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 27-108, INTERACTION WITH STE11.
[10]	"Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction." Kwan J.J., Warner N., Maini J., Chan Tung K.W., Zakaria H., Pawson T., Donaldson L.W. J. Mol. Biol. 356:142-154(2006) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 32-107, INTERACTION WITH STE11.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z11116 Genomic DNA. Translation: CAA77462.1.
S54312 Genomic DNA. Translation: AAA13629.1.
X59720 Genomic DNA. Translation: CAA42384.1.
BK006937 Genomic DNA. Translation: DAA07452.1.

PIR

S17475.

RefSeq

NP_009898.1. NM_001178677.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UQV	NMR	-	A	27-108	[»]
1Z1V	NMR	-	A	32-107	[»]

ProteinModelPortal

P25344.

SMR

P25344. Positions 27-108.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-667N.

IntAct

P25344. 35 interactions.

MINT

MINT-408633.

STRING

4932.YCL032W.

Proteomic databases

PaxDb

P25344.

PeptideAtlas

P25344.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YCL032W; YCL032W; YCL032W.

GeneID

850325.

KEGG

sce:YCL032W.

Organism-specific databases

CYGD

YCL032w.

SGD

S000000537. STE50.

Phylogenomic databases

eggNOG

NOG46436.

HOGENOM

HOG000057098.

OMA

GLHPAIM.

OrthoDB

EOG4TF3VR.

Gene expression databases

Genevestigator

P25344.

GermOnline

YCL032W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.150.50. 1 hit.

InterPro

IPR000159. Ras-assoc.
IPR013761. SAM/pointed.
IPR015316. SAM_Ste50p.
[Graphical view]

Pfam

PF00788. RA. 1 hit.
PF09235. Ste50p-SAM. 1 hit.
[Graphical view]

SMART

SM00314. RA. 1 hit.
[Graphical view]

SUPFAM

SSF47769. SAM_homology. 1 hit.

PROSITE

PS50200. RA. 1 hit.
PS50105. SAM_DOMAIN. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P25344.

NextBio

965745.

Entry information

Entry name

STE50_YEAST

Accession

Primary (citable) accession number: P25344
Secondary accession number(s): D6VQY3, Q3S1N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program