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P25344

- STE50_YEAST

UniProt

P25344 - STE50_YEAST

Protein

Protein STE50

Gene

STE50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Involved in growth arrest during conjugation. May interact with the G protein alpha subunit.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein kinase regulator activity Source: SGD
    3. SAM domain binding Source: SGD

    GO - Biological processi

    1. cell cycle arrest Source: UniProtKB-KW
    2. cellular response to heat Source: SGD
    3. MAPK cascade involved in osmosensory signaling pathway Source: SGD
    4. osmosensory signaling pathway via Sho1 osmosensor Source: SGD
    5. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
    6. regulation of protein kinase activity Source: GOC
    7. signal transduction involved in filamentous growth Source: SGD

    Keywords - Biological processi

    Cell cycle, Growth arrest, Pheromone response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29292-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein STE50
    Gene namesi
    Name:STE50
    Ordered Locus Names:YCL032W
    ORF Names:YCL32W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL032w.
    SGDiS000000537. STE50.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 346346Protein STE50PRO_0000072265Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei202 – 2021Phosphoserine3 Publications
    Modified residuei244 – 2441Phosphothreonine1 Publication
    Modified residuei248 – 2481Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP25344.
    PaxDbiP25344.
    PeptideAtlasiP25344.

    Expressioni

    Gene expression databases

    GenevestigatoriP25344.

    Interactioni

    Subunit structurei

    Interacts with STE11 through the respective SAM domains.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SHO1P400733EBI-18305,EBI-18140
    STE11P235616EBI-18305,EBI-18259

    Protein-protein interaction databases

    BioGridi30951. 384 interactions.
    DIPiDIP-667N.
    IntActiP25344. 37 interactions.
    MINTiMINT-408633.
    STRINGi4932.YCL032W.

    Structurei

    Secondary structure

    1
    346
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni32 – 343
    Helixi37 – 4812
    Helixi56 – 627
    Helixi67 – 693
    Helixi70 – 8011
    Beta strandi82 – 843
    Helixi86 – 10116

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UQVNMR-A27-108[»]
    1Z1VNMR-A32-107[»]
    ProteinModelPortaliP25344.
    SMRiP25344. Positions 27-108.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25344.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 10885SAMAdd
    BLAST
    Domaini233 – 32795Ras-associatingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Ras-associating domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG46436.
    HOGENOMiHOG000057098.
    OMAiGLHPAIM.
    OrthoDBiEOG7GFBFJ.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    InterProiIPR000159. Ras-assoc.
    IPR013761. SAM/pointed.
    IPR015316. SAM_Ste50p.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00788. RA. 1 hit.
    PF09235. Ste50p-SAM. 1 hit.
    [Graphical view]
    SMARTiSM00314. RA. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50200. RA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25344-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV    50
    EETDPLCQRL RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR 100
    DSKLEWKDDK TQEDMITVLK NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT 150
    SSSSSPINTH GVSTTVPSSN NTIIPSSDGV SLSQTDYFDT VHNRQSPSRR 200
    ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS TANTPGPSPN 250
    EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL 300
    NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL 346
    Length:346
    Mass (Da):38,971
    Last modified:May 1, 1992 - v1
    Checksum:iEFC7D14F2940C779
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11116 Genomic DNA. Translation: CAA77462.1.
    S54312 Genomic DNA. Translation: AAA13629.1.
    X59720 Genomic DNA. Translation: CAA42384.1.
    BK006937 Genomic DNA. Translation: DAA07452.1.
    PIRiS17475.
    RefSeqiNP_009898.1. NM_001178677.1.

    Genome annotation databases

    EnsemblFungiiYCL032W; YCL032W; YCL032W.
    GeneIDi850325.
    KEGGisce:YCL032W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11116 Genomic DNA. Translation: CAA77462.1 .
    S54312 Genomic DNA. Translation: AAA13629.1 .
    X59720 Genomic DNA. Translation: CAA42384.1 .
    BK006937 Genomic DNA. Translation: DAA07452.1 .
    PIRi S17475.
    RefSeqi NP_009898.1. NM_001178677.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UQV NMR - A 27-108 [» ]
    1Z1V NMR - A 32-107 [» ]
    ProteinModelPortali P25344.
    SMRi P25344. Positions 27-108.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30951. 384 interactions.
    DIPi DIP-667N.
    IntActi P25344. 37 interactions.
    MINTi MINT-408633.
    STRINGi 4932.YCL032W.

    Proteomic databases

    MaxQBi P25344.
    PaxDbi P25344.
    PeptideAtlasi P25344.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL032W ; YCL032W ; YCL032W .
    GeneIDi 850325.
    KEGGi sce:YCL032W.

    Organism-specific databases

    CYGDi YCL032w.
    SGDi S000000537. STE50.

    Phylogenomic databases

    eggNOGi NOG46436.
    HOGENOMi HOG000057098.
    OMAi GLHPAIM.
    OrthoDBi EOG7GFBFJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29292-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P25344.
    NextBioi 965745.

    Gene expression databases

    Genevestigatori P25344.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    InterProi IPR000159. Ras-assoc.
    IPR013761. SAM/pointed.
    IPR015316. SAM_Ste50p.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00788. RA. 1 hit.
    PF09235. Ste50p-SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00314. RA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50200. RA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae."
      Rad M.R., Xu G., Hollenberg C.P.
      Mol. Gen. Genet. 236:145-154(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
      Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
      Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202; THR-244 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM."
      Grimshaw S.J., Mott H.R., Stott K.M., Nielsen P.R., Evetts K.A., Hopkins L.J., Nietlispach D., Owen D.
      J. Biol. Chem. 279:2192-2201(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 27-108, INTERACTION WITH STE11.
    10. "Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction."
      Kwan J.J., Warner N., Maini J., Chan Tung K.W., Zakaria H., Pawson T., Donaldson L.W.
      J. Mol. Biol. 356:142-154(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 32-107, INTERACTION WITH STE11.

    Entry informationi

    Entry nameiSTE50_YEAST
    AccessioniPrimary (citable) accession number: P25344
    Secondary accession number(s): D6VQY3, Q3S1N2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1670 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3