P25343 (RV161_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reduced viability upon starvation protein 161

Gene names

Name:	RVS161
Synonyms:	END6, SPE161
Ordered Locus Names:	YCR009C
ORF Names:	YCR9C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of a cytoskeletal structure that is required for the formation of endocytic vesicles at the plasma membrane level.
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	Mutations in this gene results in sensitivity to carbon, nitrogen and sulfur starvation. Present with 7390 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 BAR domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin cortical patch localization Inferred from mutant phenotype. Source: SGD actin cytoskeleton reorganization Inferred from mutant phenotype. Source: SGD conjugation with cellular fusion Inferred from mutant phenotype. Source: SGD endocytosis Inferred from mutant phenotype. Source: SGD lipid tube assembly Inferred from direct assay. Source: SGD response to osmotic stress Inferred from mutant phenotype Ref.5. Source: SGD response to starvation Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	actin cortical patch Inferred from direct assay. Source: SGD mating projection tip Inferred from direct assay. Source: SGD membrane raft Inferred from direct assay. Source: SGD
Molecular function	cytoskeletal protein binding Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RVS167	P39743	16	EBI-14490,EBI-14500

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Reduced viability upon starvation protein 161		PRO_0000192960
Regions
	Domain	15 – 239	225	BAR
	Coiled coil	126 – 193	68	Potential
Amino acid modifications
	Modified residue	16	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	95	1	Q → E in CAA44926. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25343 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: B35EA00E0C900E2D	FASTA	265	30,250
	10 20 30 40 50 60 MSWEGFKKAI NRAGHSVIIK NVDKTIDKEY DMEERRYKVL QRAGEALQKE AKGFLDSLRA 70 80 90 100 110 120 VTASQTTIAE VISNLYDDSK YVAGGGYNVG NYYLQCVQDF DSETVKQLDG PLRETVLDPI 130 140 150 160 170 180 TKFSTYFKEI EEAIKKRDHK KQDFDAAKAK VRRLVDKPAK DASKLPRAEK ELSLAKDIFE 190 200 210 220 230 240 NLNNQLKTEL PQLVSLRVPY FDPSFEALIK IQLRFCTDGY TRLAQIQQYL DQQSRDDYAN 250 260 GLLDTKIEEL LGQMTSLDIC ALGIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the yeast gene RVS 161 located on chromosome III." Urdaci M., Dulau L., Aigle M., Crouzet M. Yeast 6:173-176(1990) [PubMed: 2183524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 44827 / SKQ2N.
[2]	"The complete sequence of a 10.8 kb segment distal of SUF2 on the right arm of chromosome III from Saccharomyces cerevisiae reveals seven open reading frames including the RVS161, ADP1 and PGK genes." Skala J., Purnelle B., Goffeau A. Yeast 8:409-417(1992) [PubMed: 1626432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast mutant affected for viability upon nutrient starvation: characterization and cloning of the RVS161 gene." Crouzet M., Urdaci M., Dulau L., Aigle M. Yeast 7:727-743(1991) [PubMed: 1776363] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 44827 / SKQ2N.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63315 Genomic DNA. Translation: CAA44926.1. X59720 Genomic DNA. Translation: CAA42326.1. BK006937 Genomic DNA. Translation: DAA07487.1.
PIR	S19516.
RefSeq	NP_009935.1. NM_001178722.1.
3D structure databases
ProteinModelPortal	P25343.
SMR	P25343. Positions 48-237.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1601N.
IntAct	P25343. 25 interactions.
MINT	MINT-370070.
STRING	P25343.
Protein family/group databases
TCDB	8.A.34.2.1. endophilin family.
Proteomic databases
PeptideAtlas	P25343.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR009C; YCR009C; YCR009C.
GeneID	850367.
KEGG	sce:YCR009C.
NMPDR	fig|4932.3.peg.658.
Organism-specific databases
CYGD	YCR009c.
SGD	S000000602. RVS161.
Phylogenomic databases
eggNOG	fuNOG05385.
GeneTree	EFGT00050000000908.
HOGENOM	HBG328963.
OMA	YEQLNEQ.
OrthoDB	EOG4QJVXH.
Gene expression databases
ArrayExpress	P25343.
Genevestigator	P25343.
GermOnline	YCR009C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010504. Arfaptin_homology_dom. IPR004148. BAR_dom. [Graphical view]
Gene3D	G3DSA:1.20.1270.60. Arfaptin. 1 hit.
Pfam	PF03114. BAR. 1 hit. [Graphical view]
SMART	SM00721. BAR. 1 hit. [Graphical view]
PROSITE	PS51021. BAR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	965852.

Entry information

Entry name

RV161_YEAST

Accession

Primary (citable) accession number: P25343
Secondary accession number(s): D6VR18

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program