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P25342

- CDC10_YEAST

UniProt

P25342 - CDC10_YEAST

Protein

Cell division control protein 10

Gene

CDC10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Septins are GTPases involved in cytokinesis that assemble early in the cell cycle as a patch at the incipient bud site and form a ring approximate 15 minutes before bud emergence, which transforms into an hour-glass shaped collar of cortical filaments that spans both sides of the mother-bud neck. This collar persists until just before cytokinesis, when it splits into two rings that occupy opposite sides of the neck. The septins at the bud neck serve as a structural scaffold that recruits different components involved in diverse processes at specific stages during the cell cycle. Many proteins bind asymmetrically to the septin collar. The septin assembly is regulated by protein kinases GIN4 and/or CLA4. May act by recruiting MYO1 and HOF1, a protein involved in septation, to the site of cleavage. Septins are also involved in cell morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei74 – 741GTPBy similarity
    Binding sitei100 – 1001GTP; via amide nitrogenBy similarity
    Binding sitei236 – 2361GTP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei251 – 2511GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 468GTPBy similarity
    Nucleotide bindingi180 – 1889GTPBy similarity

    GO - Molecular functioni

    1. 1-phosphatidylinositol binding Source: SGD
    2. GTPase activity Source: SGD
    3. GTP binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. protein complex scaffold Source: SGD
    6. structural molecule activity Source: SGD

    GO - Biological processi

    1. exit from mitosis Source: SGD
    2. GTP catabolic process Source: GOC
    3. maintenance of cell polarity Source: SGD
    4. septin ring assembly Source: SGD
    5. sporulation Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29321-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division control protein 10
    Gene namesi
    Name:CDC10
    Ordered Locus Names:YCR002C
    ORF Names:YCR022, YCR2C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR002c.
    SGDiS000000595. CDC10.

    Subcellular locationi

    Membrane 1 Publication; Peripheral membrane protein 1 Publication. Bud neck 1 Publication
    Note: Present at the bud neck during cell division. Probably interacts with phosphoinosides such as phosphatidylinositol 4-phosphate or phosphatidylinositol 5-phosphate.

    GO - Cellular componenti

    1. ascospore-type prospore Source: SGD
    2. cellular bud neck septin ring Source: SGD
    3. mating projection base Source: SGD
    4. meiotic spindle Source: SGD
    5. prospore membrane Source: SGD
    6. septin complex Source: SGD
    7. septin filament array Source: SGD
    8. spindle microtubule Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322Cell division control protein 10PRO_0000173498Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei216 – 2161Phosphothreonine2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP25342.
    PaxDbiP25342.
    PeptideAtlasiP25342.

    Expressioni

    Gene expression databases

    GenevestigatoriP25342.

    Interactioni

    Subunit structurei

    Component of the septin complex which consists of CDC3, CDC10, CDC11, CDC12 and probably SHS1 and rearranges to a cortical collar of highly ordered filaments at the mother-bud-neck. A complex formed by CDC3, CDC10, CDC11 and CDC12 is capable of forming long filaments in vitro and the components seem to be present in a 2:2:2:2 arrangement in vivo. The filaments are proposed to be formed by the end-to-end polymerization of CDC3-CDC12-CDC11 complexes with CDC10 serving as a bridge to bundle the polymers into paired filaments. Component of the GIN4 complex composed of at least BNI5, CDC3, CDC10, CDC11, CDC12, GIN4, NAP1 and SHS1. Self-associates. Interacts with SYP1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUD4P471362EBI-4174,EBI-3848

    Protein-protein interaction databases

    BioGridi30980. 186 interactions.
    DIPiDIP-673N.
    IntActiP25342. 13 interactions.
    MINTiMINT-619481.
    STRINGi4932.YCR002C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25342.
    SMRiP25342. Positions 31-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 302274Septin-type GAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5019.
    GeneTreeiENSGT00740000115641.
    HOGENOMiHOG000233586.
    KOiK16938.
    OMAiRKELTAM.
    OrthoDBiEOG76HQBH.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR000038. Cell_div_GTP-bd.
    IPR027417. P-loop_NTPase.
    IPR016491. Septin.
    [Graphical view]
    PANTHERiPTHR18884. PTHR18884. 1 hit.
    PfamiPF00735. Septin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006698. Septin. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS51719. G_SEPTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25342-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPLSSVQPA SYVGFDTITN QIEHRLLKKG FQFNIMVVGQ SGLGKSTLIN    50
    TLFASHLIDS ATGDDISALP VTKTTEMKIS THTLVEDRVR LNINVIDTPG 100
    FGDFIDNSKA WEPIVKYIKE QHSQYLRKEL TAQRERFITD TRVHAILYFL 150
    QPNGKELSRL DVEALKRLTE IANVIPVIGK SDTLTLDERT EFRELIQNEF 200
    EKYNFKIYPY DSEELTDEEL ELNRSVRSII PFAVVGSENE IEINGETFRG 250
    RKTRWSAINV EDINQCDFVY LREFLIRTHL QDLIETTSYI HYEGFRARQL 300
    IALKENANSR SSAHMSSNAI QR 322
    Length:322
    Mass (Da):37,025
    Last modified:May 1, 1992 - v1
    Checksum:i672467301D4D2697
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16549 Genomic DNA. Translation: AAB49377.1.
    S48552 Genomic DNA. Translation: AAD13856.1.
    X59720 Genomic DNA. Translation: CAA42339.1.
    BK006937 Genomic DNA. Translation: DAA07481.1.
    PIRiS19441.
    RefSeqiNP_009928.1. NM_001178715.1.

    Genome annotation databases

    EnsemblFungiiYCR002C; YCR002C; YCR002C.
    GeneIDi850358.
    KEGGisce:YCR002C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16549 Genomic DNA. Translation: AAB49377.1 .
    S48552 Genomic DNA. Translation: AAD13856.1 .
    X59720 Genomic DNA. Translation: CAA42339.1 .
    BK006937 Genomic DNA. Translation: DAA07481.1 .
    PIRi S19441.
    RefSeqi NP_009928.1. NM_001178715.1.

    3D structure databases

    ProteinModelPortali P25342.
    SMRi P25342. Positions 31-300.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30980. 186 interactions.
    DIPi DIP-673N.
    IntActi P25342. 13 interactions.
    MINTi MINT-619481.
    STRINGi 4932.YCR002C.

    Proteomic databases

    MaxQBi P25342.
    PaxDbi P25342.
    PeptideAtlasi P25342.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR002C ; YCR002C ; YCR002C .
    GeneIDi 850358.
    KEGGi sce:YCR002C.

    Organism-specific databases

    CYGDi YCR002c.
    SGDi S000000595. CDC10.

    Phylogenomic databases

    eggNOGi COG5019.
    GeneTreei ENSGT00740000115641.
    HOGENOMi HOG000233586.
    KOi K16938.
    OMAi RKELTAM.
    OrthoDBi EOG76HQBH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29321-MONOMER.

    Miscellaneous databases

    NextBioi 965830.

    Gene expression databases

    Genevestigatori P25342.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR000038. Cell_div_GTP-bd.
    IPR027417. P-loop_NTPase.
    IPR016491. Septin.
    [Graphical view ]
    PANTHERi PTHR18884. PTHR18884. 1 hit.
    Pfami PF00735. Septin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006698. Septin. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS51719. G_SEPTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26109 / X2180.
    2. "Sequence of the CDC10 region at chromosome III of Saccharomyces cerevisiae."
      Steensma H.Y., van der Aart Q.J.M.
      Yeast 7:425-429(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function."
      Frazier J.A., Wong M.L., Longtine M.S., Pringle J.R., Mann M., Mitchison T.J., Field C.
      J. Cell Biol. 143:737-749(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SEPTIN COMPLEX.
    6. "Cell cycle-dependent assembly of a Gin4-septin complex."
      Mortensen E.M., McDonald H., Yates J. III, Kellogg D.R.
      Mol. Biol. Cell 13:2091-2105(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE GIN4 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function."
      Casamayor A., Snyder M.
      Mol. Cell. Biol. 23:2762-2777(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH PHOSPHOINOSIDES LIPIDS, INTERACTION WITH CDC11.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Protein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae."
      Versele M., Gullbrand B., Shulewitz M.J., Cid V.J., Bahmanyar S., Chen R.E., Barth P., Alber T., Thorner J.
      Mol. Biol. Cell 15:4568-4583(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SELF-ASSOCIATION, ASSEMBLY OF THE SEPTIN FILAMENTS.
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast."
      Qiu W., Neo S.P., Yu X., Cai M.
      Genetics 180:1445-1457(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYP1.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDC10_YEAST
    AccessioniPrimary (citable) accession number: P25342
    Secondary accession number(s): D6VR12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 147 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 14100 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3