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P25341

- KIN82_YEAST

UniProt

P25341 - KIN82_YEAST

Protein

Serine/threonine-protein kinase KIN82

Gene

KIN82

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Flippase activator that phosphorylates DFN1 and DFN2 and which is involved in the generation of phospholipid asymmetry in membranes by the inward translocation of phospholipids.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei353 – 3531ATPPROSITE-ProRule annotation
    Active sitei449 – 4491Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi330 – 3389ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: SGD
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. phospholipid translocation Source: SGD
    2. protein phosphorylation Source: SGD
    3. response to pheromone Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Lipid transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29385-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase KIN82 (EC:2.7.11.1)
    Alternative name(s):
    Flippase kinase 2
    Gene namesi
    Name:KIN82
    Synonyms:FPK2
    Ordered Locus Names:YCR091W
    ORF Names:YCR1153, YCR91W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR091w.
    SGDiS000000687. KIN82.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 720720Serine/threonine-protein kinase KIN82PRO_0000086136Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei203 – 2031Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP25341.

    Interactioni

    Protein-protein interaction databases

    BioGridi31063. 101 interactions.
    DIPiDIP-6460N.
    IntActiP25341. 9 interactions.
    MINTiMINT-617837.
    STRINGi4932.YCR091W.

    Structurei

    3D structure databases

    ProteinModelPortaliP25341.
    SMRiP25341. Positions 264-693.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini324 – 602279Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KIN82 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000063286.
    HOGENOMiHOG000175846.
    KOiK08286.
    OMAiDENGANP.
    OrthoDBiEOG7R2BT5.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25341-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQQEYRSPS QRLSKGRSMS LPKIFARNLR SLQNNAPPGK NINVNCLNVN    50
    SCSLSASPSS QINMACNGNK QDLPIPFPLH VECNDSWSSS KLNKFKSMFN 100
    HNRSKSSGTT DASTSEKGTH KREPRSTIHT ELLQSSIIGE PNVHSTTSST 150
    LIPNEAICST PNEISGSSSP DAELFTFDMP TDPSSFHTPS SPSYIAKDSR 200
    NLSNGSLNDI NENEELQNFH RKISENGSAS PLANLSLSNS PIDSPRKNSE 250
    TRKDQIPMNI TPRLRRAASE PFNTAKDGLM REDYIALKQP PSLGDIVEPR 300
    RSRRLRTKSF GNKFQDITVE PQSFEKIRLL GQGDVGKVYL VRERDTNQIF 350
    ALKVLNKHEM IKRKKIKRVL TEQEILATSD HPFIVTLYHS FQTKDYLYLC 400
    MEYCMGGEFF RALQTRKSKC IAEEDAKFYA SEVVAALEYL HLLGFIYRDL 450
    KPENILLHQS GHVMLSDFDL SIQATGSKKP TMKDSTYLDT KICSDGFRTN 500
    SFVGTEEYLA PEVIRGNGHT AAVDWWTLGI LIYEMLFGCT PFKGDNSNET 550
    FSNILTKDVK FPHDKEVSKN CKDLIKKLLN KNEAKRLGSK SGAADIKRHP 600
    FFKKVQWSFL RNQDPPLIPA LNDNGCELPF ILSCNKHPKR NSVSEQETKM 650
    FCEKVANDDE IDEADPFHDF NSMSLTKKDH NILTYSENYT YGKILYKATC 700
    TRPRHNSSHR SFFKDIIPEL 720
    Length:720
    Mass (Da):81,462
    Last modified:July 27, 2011 - v3
    Checksum:iFBE3C038C18605E0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti341 – 3411V → M no nucleotide entry (PubMed:1580103)Curated
    Sequence conflicti341 – 3411V → M in CAA42256. (PubMed:1574125)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42256.2.
    BK006937 Genomic DNA. Translation: DAA07560.2.
    PIRiS22258.
    RefSeqiNP_010015.3. NM_001178797.2.

    Genome annotation databases

    EnsemblFungiiYCR091W; YCR091W; YCR091W.
    GeneIDi850453.
    KEGGisce:YCR091W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42256.2 .
    BK006937 Genomic DNA. Translation: DAA07560.2 .
    PIRi S22258.
    RefSeqi NP_010015.3. NM_001178797.2.

    3D structure databases

    ProteinModelPortali P25341.
    SMRi P25341. Positions 264-693.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31063. 101 interactions.
    DIPi DIP-6460N.
    IntActi P25341. 9 interactions.
    MINTi MINT-617837.
    STRINGi 4932.YCR091W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR091W ; YCR091W ; YCR091W .
    GeneIDi 850453.
    KEGGi sce:YCR091W.

    Organism-specific databases

    CYGDi YCR091w.
    SGDi S000000687. KIN82.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000063286.
    HOGENOMi HOG000175846.
    KOi K08286.
    OMAi DENGANP.
    OrthoDBi EOG7R2BT5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29385-MONOMER.

    Miscellaneous databases

    NextBioi 966075.

    Gene expression databases

    Genevestigatori P25341.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A putative serine/threonine protein kinase gene on chromosome III of Saccharomyces cerevisiae."
      Wilson C., Bergantino E., Lanfranchi G., Valle G., Carignani G., Frontali L.
      Yeast 8:71-77(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 341 AND C-TERMINUS.
    4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 341.
      Strain: ATCC 204508 / S288c.
    5. "Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry."
      Nakano K., Yamamoto T., Kishimoto T., Noji T., Tanaka K.
      Mol. Biol. Cell 19:1783-1797(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKIN82_YEAST
    AccessioniPrimary (citable) accession number: P25341
    Secondary accession number(s): D6VR91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 131 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3