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Protein

Serine/threonine-protein kinase KIN82

Gene

KIN82

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Flippase activator that phosphorylates DFN1 and DFN2 and which is involved in the generation of phospholipid asymmetry in membranes by the inward translocation of phospholipids.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei353ATPPROSITE-ProRule annotation1
Active sitei449Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi330 – 338ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • phospholipid translocation Source: SGD
  • protein phosphorylation Source: SGD
  • response to pheromone triggering conjugation with cellular fusion Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processLipid transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29385-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase KIN82 (EC:2.7.11.1)
Alternative name(s):
Flippase kinase 2
Gene namesi
Name:KIN82
Synonyms:FPK2
Ordered Locus Names:YCR091W
ORF Names:YCR1153, YCR91W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR091W
SGDiS000000687 KIN82

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861361 – 720Serine/threonine-protein kinase KIN82Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25341
PaxDbiP25341
PRIDEiP25341

PTM databases

iPTMnetiP25341

Interactioni

Protein-protein interaction databases

BioGridi31063, 149 interactors
DIPiDIP-6460N
IntActiP25341, 10 interactors
MINTiP25341
STRINGi4932.YCR091W

Structurei

3D structure databases

ProteinModelPortaliP25341
SMRiP25341
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini324 – 602Protein kinasePROSITE-ProRule annotationAdd BLAST279

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KIN82 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00900000142150
HOGENOMiHOG000175846
InParanoidiP25341
KOiK08286
OMAiSHIGTIN
OrthoDBiEOG092C0HH3

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P25341-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQQEYRSPS QRLSKGRSMS LPKIFARNLR SLQNNAPPGK NINVNCLNVN
60 70 80 90 100
SCSLSASPSS QINMACNGNK QDLPIPFPLH VECNDSWSSS KLNKFKSMFN
110 120 130 140 150
HNRSKSSGTT DASTSEKGTH KREPRSTIHT ELLQSSIIGE PNVHSTTSST
160 170 180 190 200
LIPNEAICST PNEISGSSSP DAELFTFDMP TDPSSFHTPS SPSYIAKDSR
210 220 230 240 250
NLSNGSLNDI NENEELQNFH RKISENGSAS PLANLSLSNS PIDSPRKNSE
260 270 280 290 300
TRKDQIPMNI TPRLRRAASE PFNTAKDGLM REDYIALKQP PSLGDIVEPR
310 320 330 340 350
RSRRLRTKSF GNKFQDITVE PQSFEKIRLL GQGDVGKVYL VRERDTNQIF
360 370 380 390 400
ALKVLNKHEM IKRKKIKRVL TEQEILATSD HPFIVTLYHS FQTKDYLYLC
410 420 430 440 450
MEYCMGGEFF RALQTRKSKC IAEEDAKFYA SEVVAALEYL HLLGFIYRDL
460 470 480 490 500
KPENILLHQS GHVMLSDFDL SIQATGSKKP TMKDSTYLDT KICSDGFRTN
510 520 530 540 550
SFVGTEEYLA PEVIRGNGHT AAVDWWTLGI LIYEMLFGCT PFKGDNSNET
560 570 580 590 600
FSNILTKDVK FPHDKEVSKN CKDLIKKLLN KNEAKRLGSK SGAADIKRHP
610 620 630 640 650
FFKKVQWSFL RNQDPPLIPA LNDNGCELPF ILSCNKHPKR NSVSEQETKM
660 670 680 690 700
FCEKVANDDE IDEADPFHDF NSMSLTKKDH NILTYSENYT YGKILYKATC
710 720
TRPRHNSSHR SFFKDIIPEL
Length:720
Mass (Da):81,462
Last modified:July 27, 2011 - v3
Checksum:iFBE3C038C18605E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341V → M no nucleotide entry (PubMed:1580103).Curated1
Sequence conflicti341V → M in CAA42256 (PubMed:1574125).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42256.2
BK006937 Genomic DNA Translation: DAA07560.2
PIRiS22258
RefSeqiNP_010015.3, NM_001178797.2

Genome annotation databases

EnsemblFungiiCAA42256; CAA42256; CAA42256
YCR091W; YCR091W; YCR091W
GeneIDi850453
KEGGisce:YCR091W

Similar proteinsi

Entry informationi

Entry nameiKIN82_YEAST
AccessioniPrimary (citable) accession number: P25341
Secondary accession number(s): D6VR91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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