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P25341

- KIN82_YEAST

UniProt

P25341 - KIN82_YEAST

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Protein

Serine/threonine-protein kinase KIN82

Gene

KIN82

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Flippase activator that phosphorylates DFN1 and DFN2 and which is involved in the generation of phospholipid asymmetry in membranes by the inward translocation of phospholipids.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei353 – 3531ATPPROSITE-ProRule annotation
Active sitei449 – 4491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi330 – 3389ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase activity Source: SGD
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. phospholipid translocation Source: SGD
  2. protein phosphorylation Source: SGD
  3. response to pheromone Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29385-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase KIN82 (EC:2.7.11.1)
Alternative name(s):
Flippase kinase 2
Gene namesi
Name:KIN82
Synonyms:FPK2
Ordered Locus Names:YCR091W
ORF Names:YCR1153, YCR91W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR091w.
SGDiS000000687. KIN82.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Serine/threonine-protein kinase KIN82PRO_0000086136Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei203 – 2031Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

GenevestigatoriP25341.

Interactioni

Protein-protein interaction databases

BioGridi31063. 101 interactions.
DIPiDIP-6460N.
IntActiP25341. 9 interactions.
MINTiMINT-617837.
STRINGi4932.YCR091W.

Structurei

3D structure databases

ProteinModelPortaliP25341.
SMRiP25341. Positions 264-693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini324 – 602279Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KIN82 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063286.
HOGENOMiHOG000175846.
InParanoidiP25341.
KOiK08286.
OMAiDENGANP.
OrthoDBiEOG7R2BT5.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25341-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQQEYRSPS QRLSKGRSMS LPKIFARNLR SLQNNAPPGK NINVNCLNVN
60 70 80 90 100
SCSLSASPSS QINMACNGNK QDLPIPFPLH VECNDSWSSS KLNKFKSMFN
110 120 130 140 150
HNRSKSSGTT DASTSEKGTH KREPRSTIHT ELLQSSIIGE PNVHSTTSST
160 170 180 190 200
LIPNEAICST PNEISGSSSP DAELFTFDMP TDPSSFHTPS SPSYIAKDSR
210 220 230 240 250
NLSNGSLNDI NENEELQNFH RKISENGSAS PLANLSLSNS PIDSPRKNSE
260 270 280 290 300
TRKDQIPMNI TPRLRRAASE PFNTAKDGLM REDYIALKQP PSLGDIVEPR
310 320 330 340 350
RSRRLRTKSF GNKFQDITVE PQSFEKIRLL GQGDVGKVYL VRERDTNQIF
360 370 380 390 400
ALKVLNKHEM IKRKKIKRVL TEQEILATSD HPFIVTLYHS FQTKDYLYLC
410 420 430 440 450
MEYCMGGEFF RALQTRKSKC IAEEDAKFYA SEVVAALEYL HLLGFIYRDL
460 470 480 490 500
KPENILLHQS GHVMLSDFDL SIQATGSKKP TMKDSTYLDT KICSDGFRTN
510 520 530 540 550
SFVGTEEYLA PEVIRGNGHT AAVDWWTLGI LIYEMLFGCT PFKGDNSNET
560 570 580 590 600
FSNILTKDVK FPHDKEVSKN CKDLIKKLLN KNEAKRLGSK SGAADIKRHP
610 620 630 640 650
FFKKVQWSFL RNQDPPLIPA LNDNGCELPF ILSCNKHPKR NSVSEQETKM
660 670 680 690 700
FCEKVANDDE IDEADPFHDF NSMSLTKKDH NILTYSENYT YGKILYKATC
710 720
TRPRHNSSHR SFFKDIIPEL
Length:720
Mass (Da):81,462
Last modified:July 27, 2011 - v3
Checksum:iFBE3C038C18605E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3411V → M no nucleotide entry (PubMed:1580103)Curated
Sequence conflicti341 – 3411V → M in CAA42256. (PubMed:1574125)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42256.2.
BK006937 Genomic DNA. Translation: DAA07560.2.
PIRiS22258.
RefSeqiNP_010015.3. NM_001178797.2.

Genome annotation databases

EnsemblFungiiYCR091W; YCR091W; YCR091W.
GeneIDi850453.
KEGGisce:YCR091W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42256.2 .
BK006937 Genomic DNA. Translation: DAA07560.2 .
PIRi S22258.
RefSeqi NP_010015.3. NM_001178797.2.

3D structure databases

ProteinModelPortali P25341.
SMRi P25341. Positions 264-693.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31063. 101 interactions.
DIPi DIP-6460N.
IntActi P25341. 9 interactions.
MINTi MINT-617837.
STRINGi 4932.YCR091W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR091W ; YCR091W ; YCR091W .
GeneIDi 850453.
KEGGi sce:YCR091W.

Organism-specific databases

CYGDi YCR091w.
SGDi S000000687. KIN82.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063286.
HOGENOMi HOG000175846.
InParanoidi P25341.
KOi K08286.
OMAi DENGANP.
OrthoDBi EOG7R2BT5.

Enzyme and pathway databases

BioCyci YEAST:G3O-29385-MONOMER.

Miscellaneous databases

NextBioi 966075.

Gene expression databases

Genevestigatori P25341.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A putative serine/threonine protein kinase gene on chromosome III of Saccharomyces cerevisiae."
    Wilson C., Bergantino E., Lanfranchi G., Valle G., Carignani G., Frontali L.
    Yeast 8:71-77(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Valles G., Volckaerts G.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 341 AND C-TERMINUS.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 341.
    Strain: ATCC 204508 / S288c.
  5. "Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry."
    Nakano K., Yamamoto T., Kishimoto T., Noji T., Tanaka K.
    Mol. Biol. Cell 19:1783-1797(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKIN82_YEAST
AccessioniPrimary (citable) accession number: P25341
Secondary accession number(s): D6VR91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3