Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Delta(24(24(1)))-sterol reductase

Gene

ERG4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Ergosterol + NADP+ = ergosta-5,7,22,24(241)-tetraen-3-beta-ol + NADPH.

Pathwayi: ergosterol biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes ergosterol from zymosterol.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Sterol 24-C-methyltransferase (ERG6)
  2. C-8 sterol isomerase (ERG2)
  3. Delta(7)-sterol 5(6)-desaturase (ERG3)
  4. Cytochrome P450 61 (ERG5)
  5. Delta(24(24(1)))-sterol reductase (ERG4)
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

GO - Molecular functioni

  • delta24(24-1) sterol reductase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:YGL012W-MONOMER.
YEAST:YGL012W-MONOMER.
UniPathwayiUPA00768; UER00764.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(24(24(1)))-sterol reductase (EC:1.3.1.71)
Alternative name(s):
C-24(28) sterol reductase
Sterol Delta(24(28))-reductase
Gene namesi
Name:ERG4
Ordered Locus Names:YGL012W
ORF Names:YGL022
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL012W.
SGDiS000002980. ERG4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939LumenalSequence analysisAdd
BLAST
Transmembranei40 – 6425HelicalSequence analysisAdd
BLAST
Topological domaini65 – 10137CytoplasmicSequence analysisAdd
BLAST
Transmembranei102 – 12322HelicalSequence analysisAdd
BLAST
Topological domaini124 – 13815LumenalSequence analysisAdd
BLAST
Transmembranei139 – 15719HelicalSequence analysisAdd
BLAST
Topological domaini158 – 17417CytoplasmicSequence analysisAdd
BLAST
Transmembranei175 – 19723HelicalSequence analysisAdd
BLAST
Topological domaini198 – 23235LumenalSequence analysisAdd
BLAST
Transmembranei233 – 24917HelicalSequence analysisAdd
BLAST
Topological domaini250 – 32677CytoplasmicSequence analysisAdd
BLAST
Transmembranei327 – 34620HelicalSequence analysisAdd
BLAST
Topological domaini347 – 41468LumenalSequence analysisAdd
BLAST
Transmembranei415 – 43925HelicalSequence analysisAdd
BLAST
Topological domaini440 – 47334CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Delta(24(24(1)))-sterol reductasePRO_0000207491Add
BLAST

Proteomic databases

MaxQBiP25340.

Interactioni

Protein-protein interaction databases

BioGridi33234. 118 interactions.
DIPiDIP-7177N.
IntActiP25340. 13 interactions.
MINTiMINT-1364010.

Structurei

3D structure databases

ProteinModelPortaliP25340.
SMRiP25340. Positions 49-473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ERG4/ERG24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000177774.
InParanoidiP25340.
KOiK00223.
OMAiETYGYVT.
OrthoDBiEOG7T7H31.

Family and domain databases

InterProiIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamiPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEiPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKDNSEKLQ VQGEEKKSKQ PVNFLPQGKW LKPNEIEYEF GGTTGVIGML
60 70 80 90 100
IGFPLLMYYM WICAEFYHGK VALPKAGESW MHFIKHLYQL VLENGIPEKY
110 120 130 140 150
DWTIFLTFWV FQIIFYYTLP GIWTKGQPLS HLKGKQLPYF CNAMWTLYVT
160 170 180 190 200
TTLVLVLHFT NLFRLYVIID RFGRIMTCAI ISGFAFSIIL YLWTLFISHD
210 220 230 240 250
YHRMTGNHLY DFFMGAPLNP RWGILDLKMF FEVRLPWFTL YFITLGACLK
260 270 280 290 300
QWETYGYVTP QLGVVMLAHW LYANACAKGE ELIVPTWDMA YEKFGFMLIF
310 320 330 340 350
WNIAGVPYTY CHCTLYLYYH DPSEYHWSTL YNVSLYVVLL CAYYFFDTAN
360 370 380 390 400
AQKNAFRKQM SGDKTGRKTF PFLPYQILKN PKYMVTSNGS YLLIDGWYTL
410 420 430 440 450
ARKIHYTADW TQSLVWALSC GFNSVFPWFF PVFFLVVLIH RAFRDQAKCK
460 470
RKYGKDWDEY CKHCPYVFIP YVF
Length:473
Mass (Da):56,040
Last modified:October 1, 1996 - v2
Checksum:iB4BFF14559272DD6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331P → L in AAT93224 (PubMed:17322287).Curated
Sequence conflicti366 – 3661G → V (PubMed:1882555).Curated
Sequence conflicti366 – 3661G → V (PubMed:1882553).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S58126 Genomic DNA. Translation: AAD13895.1.
S57891 Genomic DNA. Translation: AAB19615.1.
Z72534 Genomic DNA. Translation: CAA96712.1.
AY693205 Genomic DNA. Translation: AAT93224.1.
BK006941 Genomic DNA. Translation: DAA08086.1.
PIRiS64014.
RefSeqiNP_011503.1. NM_001180877.1.

Genome annotation databases

EnsemblFungiiYGL012W; YGL012W; YGL012W.
GeneIDi852872.
KEGGisce:YGL012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S58126 Genomic DNA. Translation: AAD13895.1.
S57891 Genomic DNA. Translation: AAB19615.1.
Z72534 Genomic DNA. Translation: CAA96712.1.
AY693205 Genomic DNA. Translation: AAT93224.1.
BK006941 Genomic DNA. Translation: DAA08086.1.
PIRiS64014.
RefSeqiNP_011503.1. NM_001180877.1.

3D structure databases

ProteinModelPortaliP25340.
SMRiP25340. Positions 49-473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33234. 118 interactions.
DIPiDIP-7177N.
IntActiP25340. 13 interactions.
MINTiMINT-1364010.

Proteomic databases

MaxQBiP25340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL012W; YGL012W; YGL012W.
GeneIDi852872.
KEGGisce:YGL012W.

Organism-specific databases

EuPathDBiFungiDB:YGL012W.
SGDiS000002980. ERG4.

Phylogenomic databases

HOGENOMiHOG000177774.
InParanoidiP25340.
KOiK00223.
OMAiETYGYVT.
OrthoDBiEOG7T7H31.

Enzyme and pathway databases

UniPathwayiUPA00768; UER00764.
BioCyciMetaCyc:YGL012W-MONOMER.
YEAST:YGL012W-MONOMER.

Miscellaneous databases

PROiP25340.

Family and domain databases

InterProiIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamiPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEiPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The YGL022 gene encodes a putative transport protein."
    Chen W., Capieaux E., Balzi E., Goffeau A.
    Yeast 7:305-308(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 46191 / IL125-2B.
  2. "The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance."
    Chen W., Balzi E., Capieaux E., Choder M., Goffeau A.
    Yeast 7:287-299(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 46191 / IL125-2B.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway."
    Lai M.H., Bard M., Pierson C.A., Alexander J.F., Goebl M., Carter G.T., Kirsch D.R.
    Gene 140:41-49(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiERG4_YEAST
AccessioniPrimary (citable) accession number: P25340
Secondary accession number(s): D6VUC5, E9P929
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1640 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be a transport protein.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.