Delta(24(24(1)))-sterol reductase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P25340 (ERG4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Delta(24(24(1)))-sterol reductase
EC=1.3.1.71

Alternative name(s):
C-24(28) sterol reductase
Sterol Delta(24(28))-reductase

Gene names

Name:	ERG4
Ordered Locus Names:	YGL012W
ORF Names:	YGL022

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Ergosterol + NADP⁺ = ergosta-5,7,22,24(24₁)-tetraen-3-beta-ol + NADPH.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 5/5.
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1640 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the ERG4/ERG24 family.
Caution	Was originally (Ref.1) thought to be a transport protein.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ergosterol biosynthetic process Inferred from mutant phenotype Ref.6. Source: SGD
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	delta24(24-1) sterol reductase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 473

473

Delta(24(24(1)))-sterol reductase

PRO_0000207491

Regions

Topological domain

1 – 39

Lumenal Potential

Transmembrane

40 – 64

Helical; Potential

Topological domain

65 – 101

Cytoplasmic Potential

Transmembrane

102 – 123

Helical; Potential

Topological domain

124 – 138

Lumenal Potential

Transmembrane

139 – 157

Helical; Potential

Topological domain

158 – 174

Cytoplasmic Potential

Transmembrane

175 – 197

Helical; Potential

Topological domain

198 – 232

Lumenal Potential

Transmembrane

233 – 249

Helical; Potential

Topological domain

250 – 326

Cytoplasmic Potential

Transmembrane

327 – 346

Helical; Potential

Topological domain

347 – 414

Lumenal Potential

Transmembrane

415 – 439

Helical; Potential

Topological domain

440 – 473

Cytoplasmic Potential

Experimental info

Sequence conflict

P → L in AAT93224. Ref.5

Sequence conflict

366

G → V Ref.1

Sequence conflict

366

G → V Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P25340 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: B4BFF14559272DD6
Show »

FASTA

473

56,040

        10         20         30         40         50         60 
MAKDNSEKLQ VQGEEKKSKQ PVNFLPQGKW LKPNEIEYEF GGTTGVIGML IGFPLLMYYM 

        70         80         90        100        110        120 
WICAEFYHGK VALPKAGESW MHFIKHLYQL VLENGIPEKY DWTIFLTFWV FQIIFYYTLP 

       130        140        150        160        170        180 
GIWTKGQPLS HLKGKQLPYF CNAMWTLYVT TTLVLVLHFT NLFRLYVIID RFGRIMTCAI 

       190        200        210        220        230        240 
ISGFAFSIIL YLWTLFISHD YHRMTGNHLY DFFMGAPLNP RWGILDLKMF FEVRLPWFTL 

       250        260        270        280        290        300 
YFITLGACLK QWETYGYVTP QLGVVMLAHW LYANACAKGE ELIVPTWDMA YEKFGFMLIF 

       310        320        330        340        350        360 
WNIAGVPYTY CHCTLYLYYH DPSEYHWSTL YNVSLYVVLL CAYYFFDTAN AQKNAFRKQM 

       370        380        390        400        410        420 
SGDKTGRKTF PFLPYQILKN PKYMVTSNGS YLLIDGWYTL ARKIHYTADW TQSLVWALSC 

       430        440        450        460        470 
GFNSVFPWFF PVFFLVVLIH RAFRDQAKCK RKYGKDWDEY CKHCPYVFIP YVF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The YGL022 gene encodes a putative transport protein." Chen W., Capieaux E., Balzi E., Goffeau A. Yeast 7:305-308(1991) [PubMed: 1882555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 46191 / IL125-2B.
[2]	"The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance." Chen W., Balzi E., Capieaux E., Choder M., Goffeau A. Yeast 7:287-299(1991) [PubMed: 1882553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 46191 / IL125-2B.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway." Lai M.H., Bard M., Pierson C.A., Alexander J.F., Goebl M., Carter G.T., Kirsch D.R. Gene 140:41-49(1994) [PubMed: 8125337] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S58126 Genomic DNA. Translation: AAD13895.1. S57891 Genomic DNA. Translation: AAB19615.1. Z72534 Genomic DNA. Translation: CAA96712.1. AY693205 Genomic DNA. Translation: AAT93224.1. BK006941 Genomic DNA. Translation: DAA08086.1.
PIR	S64014.
RefSeq	NP_011503.1. NM_001180877.1.
3D structure databases
ProteinModelPortal	P25340.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7177N.
IntAct	P25340. 23 interactions.
MINT	MINT-1364010.
STRING	P25340.
Proteomic databases
PeptideAtlas	P25340.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL012W; YGL012W; YGL012W.
GeneID	852872.
KEGG	sce:YGL012W.
NMPDR	fig\|4932.3.peg.2611.
Organism-specific databases
CYGD	YGL012w.
SGD	S000002980. ERG4.
Phylogenomic databases
eggNOG	fuNOG07477.
HOGENOM	HBG592488.
OMA	FFEVRLP.
OrthoDB	EOG4GBBFQ.
Enzyme and pathway databases
BioCyc	MetaCyc:YGL012W-MONOMER.
Gene expression databases
ArrayExpress	P25340.
Genevestigator	P25340.
GermOnline	YGL012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
KO	K00223.
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972502.

Entry information

Entry name

ERG4_YEAST

Accession

Primary (citable) accession number: P25340
Secondary accession number(s): D6VUC5, E9P929

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents