Delta(24(24(1)))-sterol reductase - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P25340 (ERG4_YEAST)

Last modified June 16, 2009. Version 77. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Delta(24(24(1)))-sterol reductase
    EC=1.3.1.71
Alternative name(s):
    Sterol Delta(24(28))-reductase
    C-24(28) sterol reductase

Gene names

Name:	ERG4
Ordered Locus Names:	YGL012W
ORF Names:	YGL022

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	Ergosterol + NADP⁺ = ergosta-5,7,22,24(24₁)-tetraen-3-beta-ol + NADPH.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 5/5.
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1640 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the ERG4/ERG24 family.
Caution	Was originally (Ref.1) thought to be a transport protein.

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Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ergosterol biosynthetic process Traceable author statement. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	delta24(24-1) sterol reductase activity Traceable author statement. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
	P38800	1	EBI-6558,EBI-24597
AGP1	P25376	1	EBI-6558,EBI-2357
CHO1	P08456	1	EBI-6558,EBI-14055
ERG1	P32476	1	EBI-6558,EBI-6545
FMP25	Q08023	1	EBI-6558,EBI-38592
GAP1	P19145	1	EBI-6558,EBI-7314
NSG1	P38837	1	EBI-6558,EBI-24733
OST1	P41543	1	EBI-6558,EBI-12651
PMP2	P40975	1	EBI-6558,EBI-2043041
RTN1	Q04947	1	EBI-6558,EBI-38020
SAC1	P32368	1	EBI-6558,EBI-16210
SPF1	P39986	1	EBI-6558,EBI-3128
YER053C-A	Q3E7B0	1	EBI-6558,EBI-2047907

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 473

473

Delta(24(24(1)))-sterol reductase

PRO_0000207491

Regions

Topological domain

1 – 39

Lumenal Potential

Transmembrane

40 – 64

Potential

Topological domain

65 – 101

Cytoplasmic Potential

Transmembrane

102 – 123

Potential

Topological domain

124 – 138

Lumenal Potential

Transmembrane

139 – 157

Potential

Topological domain

158 – 174

Cytoplasmic Potential

Transmembrane

175 – 197

Potential

Topological domain

198 – 232

Lumenal Potential

Transmembrane

233 – 249

Potential

Topological domain

250 – 326

Cytoplasmic Potential

Transmembrane

327 – 346

Potential

Topological domain

347 – 414

Lumenal Potential

Transmembrane

415 – 439

Potential

Topological domain

440 – 473

Cytoplasmic Potential

Experimental info

Sequence conflict

366

G → V Ref.1

Sequence conflict

366

G → V Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P25340-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: B4BFF14559272DD6
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FASTA

473

56,040

        10         20         30         40         50         60 
MAKDNSEKLQ VQGEEKKSKQ PVNFLPQGKW LKPNEIEYEF GGTTGVIGML IGFPLLMYYM 

        70         80         90        100        110        120 
WICAEFYHGK VALPKAGESW MHFIKHLYQL VLENGIPEKY DWTIFLTFWV FQIIFYYTLP 

       130        140        150        160        170        180 
GIWTKGQPLS HLKGKQLPYF CNAMWTLYVT TTLVLVLHFT NLFRLYVIID RFGRIMTCAI 

       190        200        210        220        230        240 
ISGFAFSIIL YLWTLFISHD YHRMTGNHLY DFFMGAPLNP RWGILDLKMF FEVRLPWFTL 

       250        260        270        280        290        300 
YFITLGACLK QWETYGYVTP QLGVVMLAHW LYANACAKGE ELIVPTWDMA YEKFGFMLIF 

       310        320        330        340        350        360 
WNIAGVPYTY CHCTLYLYYH DPSEYHWSTL YNVSLYVVLL CAYYFFDTAN AQKNAFRKQM 

       370        380        390        400        410        420 
SGDKTGRKTF PFLPYQILKN PKYMVTSNGS YLLIDGWYTL ARKIHYTADW TQSLVWALSC 

       430        440        450        460        470 
GFNSVFPWFF PVFFLVVLIH RAFRDQAKCK RKYGKDWDEY CKHCPYVFIP YVF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The YGL022 gene encodes a putative transport protein." Chen W., Capieaux E., Balzi E., Goffeau A. Yeast 7:305-308(1991) [PubMed: 1882555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 46191 / IL125-2B.
[2]	"The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance." Chen W., Balzi E., Capieaux E., Choder M., Goffeau A. Yeast 7:287-299(1991) [PubMed: 1882553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 46191 / IL125-2B.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway." Lai M.H., Bard M., Pierson C.A., Alexander J.F., Goebl M., Carter G.T., Kirsch D.R. Gene 140:41-49(1994) [PubMed: 8125337] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	S58126 Genomic DNA. Translation: AAD13895.1. S57891 Genomic DNA. Translation: AAB19615.1. Z72534 Genomic DNA. Translation: CAA96712.1.
PIR	S64014.
RefSeq	NP_011503.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:7177N.
IntAct	P25340. 22 interactions.
Proteomic databases
PeptideAtlas	P25340.
Genome annotation databases
Ensembl	YGL012W. Saccharomyces cerevisiae. [Contig view]
GeneID	852872.
GenomeReviews	Gene locus YGL012W in contig Y13135_GR.
KEGG	sce:YGL012W.
NMPDR	fig\|4932.3.peg.2611.
Organism-specific databases
CYGD	YGL012w.
SGD	S000002980. ERG4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P25340.
OMA	P25340. DMAYEKF.
Enzyme and pathway databases
BRENDA	1.3.1.71. 250.
Gene expression databases
ArrayExpress	P25340.
GermOnline	YGL012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972502.

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Entry information

Entry name

ERG4_YEAST

Accession

Primary (citable) accession number: P25340

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents