Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pumilio homology domain family member 4

Gene

PUF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is not essential for haploid growth, but may affect diploid formation.

GO - Molecular functioni

  • mRNA binding Source: SGD

GO - Biological processi

  • negative regulation of chromatin silencing involved in replicative cell aging Source: SGD
  • negative regulation of translation Source: SGD
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD
  • protein localization Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30535-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pumilio homology domain family member 4
Gene namesi
Name:PUF4
Ordered Locus Names:YGL014W
ORF Names:YGL023
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL014W.
SGDiS000002982. PUF4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000759251 – 888Pumilio homology domain family member 4Add BLAST888

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei205PhosphothreonineCombined sources1
Modified residuei212PhosphothreonineCombined sources1
Modified residuei252PhosphothreonineCombined sources1
Modified residuei256PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25339.
PRIDEiP25339.

PTM databases

iPTMnetiP25339.

Interactioni

Protein-protein interaction databases

BioGridi33232. 101 interactors.
DIPiDIP-2638N.
IntActiP25339. 17 interactors.
MINTiMINT-425380.

Structurei

Secondary structure

1888
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni555 – 558Combined sources4
Helixi561 – 563Combined sources3
Turni564 – 566Combined sources3
Helixi568 – 571Combined sources4
Beta strandi572 – 574Combined sources3
Helixi575 – 587Combined sources13
Helixi590 – 600Combined sources11
Helixi601 – 603Combined sources3
Helixi604 – 608Combined sources5
Helixi613 – 623Combined sources11
Helixi626 – 636Combined sources11
Helixi637 – 639Combined sources3
Helixi640 – 645Combined sources6
Helixi649 – 659Combined sources11
Helixi663 – 673Combined sources11
Helixi674 – 676Combined sources3
Helixi677 – 681Combined sources5
Helixi686 – 696Combined sources11
Helixi699 – 711Combined sources13
Helixi713 – 717Combined sources5
Helixi722 – 732Combined sources11
Helixi735 – 746Combined sources12
Helixi749 – 753Combined sources5
Helixi758 – 772Combined sources15
Helixi777 – 785Combined sources9
Helixi786 – 788Combined sources3
Helixi789 – 793Combined sources5
Helixi798 – 806Combined sources9
Helixi809 – 821Combined sources13
Helixi824 – 832Combined sources9
Helixi837 – 851Combined sources15
Helixi853 – 863Combined sources11
Helixi864 – 866Combined sources3
Helixi869 – 873Combined sources5
Helixi875 – 881Combined sources7
Beta strandi883 – 885Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BWTX-ray2.69A554-886[»]
3BX2X-ray2.84A/B554-888[»]
3BX3X-ray3.00A/B554-888[»]
4DZSX-ray3.14A/B536-888[»]
ProteinModelPortaliP25339.
SMRiP25339.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25339.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini539 – 888PUM-HDPROSITE-ProRule annotationAdd BLAST350
Repeati563 – 598Pumilio 1Add BLAST36
Repeati599 – 634Pumilio 2Add BLAST36
Repeati635 – 671Pumilio 3Add BLAST37
Repeati672 – 707Pumilio 4Add BLAST36
Repeati708 – 743Pumilio 5Add BLAST36
Repeati744 – 783Pumilio 6Add BLAST40
Repeati784 – 821Pumilio 7Add BLAST38
Repeati823 – 861Pumilio 8Add BLAST39

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi8 – 38Asp/Glu-rich (acidic)Add BLAST31
Compositional biasi471 – 542Asn-richAdd BLAST72
Compositional biasi521 – 535Poly-AsnAdd BLAST15

Sequence similaritiesi

Contains 1 PUM-HD domain.PROSITE-ProRule annotation
Contains 8 pumilio repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00730000112282.
HOGENOMiHOG000115799.
InParanoidiP25339.
OMAiFRRQTFH.
OrthoDBiEOG092C0M8D.

Family and domain databases

CDDicd07920. Pumilio. 1 hit.
Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR033133. PUM-HD.
IPR033712. Pumilio_RNA-bd.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF00806. PUF. 8 hits.
[Graphical view]
SMARTiSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25339-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKGLKEEI DDVPSVDPVV SETVNSALEQ LQLDDPEENA TSNAFANKVS
60 70 80 90 100
QDSQFANGPP SQMFPHPQMM GGMGFMPYSQ MMQVPHNPCP FFPPPDFNDP
110 120 130 140 150
TAPLSSSPLN AGGPPMLFKN DSLPFQMLSS GAAVATQGGQ NLNPLINDNS
160 170 180 190 200
MKVLPIASAD PLWTHSNVPG SASVAIEETT ATLQESLPSK GRESNNKASS
210 220 230 240 250
FRRQTFHALS PTDLINAANN VTLSKDFQSD MQNFSKAKKP SVGANNTAKT
260 270 280 290 300
RTQSISFDNT PSSTSFIPPT NSVSEKLSDF KIETSKEDLI NKTAPAKKES
310 320 330 340 350
PTTYGAAYPY GGPLLQPNPI MPGHPHNISS PIYGIRSPFP NSYEMGAQFQ
360 370 380 390 400
PFSPILNPTS HSLNANSPIP LTQSPIHLAP VLNPSSNSVA FSDMKNDGGK
410 420 430 440 450
PTTDNDKAGP NVRMDLINPN LGPSMQPFHI LPPQQNTPPP PWLYSTPPPF
460 470 480 490 500
NAMVPPHLLA QNHMPLMNSA NNKHHGRNNN SMSSHNDNDN IGNSNYNNKD
510 520 530 540 550
TGRSNVGKMK NMKNSYHGYY NNNNNNNNNN NNNNNSNATN SNSAEKQRKI
560 570 580 590 600
EESSRFADAV LDQYIGSIHS LCKDQHGCRF LQKQLDILGS KAADAIFEET
610 620 630 640 650
KDYTVELMTD SFGNYLIQKL LEEVTTEQRI VLTKISSPHF VEISLNPHGT
660 670 680 690 700
RALQKLIECI KTDEEAQIVV DSLRPYTVQL SKDLNGNHVI QKCLQRLKPE
710 720 730 740 750
NFQFIFDAIS DSCIDIATHR HGCCVLQRCL DHGTTEQCDN LCDKLLALVD
760 770 780 790 800
KLTLDPFGNY VVQYIITKEA EKNKYDYTHK IVHLLKPRAI ELSIHKFGSN
810 820 830 840 850
VIEKILKTAI VSEPMILEIL NNGGETGIQS LLNDSYGNYV LQTALDISHK
860 870 880
QNDYLYKRLS EIVAPLLVGP IRNTPHGKRI IGMLHLDS
Length:888
Mass (Da):97,798
Last modified:October 1, 1996 - v2
Checksum:i659BA1062439F642
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti595A → R (PubMed:1909073).Curated1
Sequence conflicti595A → R (PubMed:1882553).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57889 Genomic DNA. Translation: AAB19616.1.
S58126 Genomic DNA. Translation: AAD13898.1.
Z72536 Genomic DNA. Translation: CAA96714.1.
BK006941 Genomic DNA. Translation: DAA08084.1.
PIRiS64016.
RefSeqiNP_011501.1. NM_001180879.1.

Genome annotation databases

EnsemblFungiiYGL014W; YGL014W; YGL014W.
GeneIDi852870.
KEGGisce:YGL014W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57889 Genomic DNA. Translation: AAB19616.1.
S58126 Genomic DNA. Translation: AAD13898.1.
Z72536 Genomic DNA. Translation: CAA96714.1.
BK006941 Genomic DNA. Translation: DAA08084.1.
PIRiS64016.
RefSeqiNP_011501.1. NM_001180879.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BWTX-ray2.69A554-886[»]
3BX2X-ray2.84A/B554-888[»]
3BX3X-ray3.00A/B554-888[»]
4DZSX-ray3.14A/B536-888[»]
ProteinModelPortaliP25339.
SMRiP25339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33232. 101 interactors.
DIPiDIP-2638N.
IntActiP25339. 17 interactors.
MINTiMINT-425380.

PTM databases

iPTMnetiP25339.

Proteomic databases

MaxQBiP25339.
PRIDEiP25339.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL014W; YGL014W; YGL014W.
GeneIDi852870.
KEGGisce:YGL014W.

Organism-specific databases

EuPathDBiFungiDB:YGL014W.
SGDiS000002982. PUF4.

Phylogenomic databases

GeneTreeiENSGT00730000112282.
HOGENOMiHOG000115799.
InParanoidiP25339.
OMAiFRRQTFH.
OrthoDBiEOG092C0M8D.

Enzyme and pathway databases

BioCyciYEAST:G3O-30535-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25339.
PROiP25339.

Family and domain databases

CDDicd07920. Pumilio. 1 hit.
Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR033133. PUM-HD.
IPR033712. Pumilio_RNA-bd.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF00806. PUF. 8 hits.
[Graphical view]
SMARTiSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUF4_YEAST
AccessioniPrimary (citable) accession number: P25339
Secondary accession number(s): D6VUC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.