Reviewed, UniProtKB/Swiss-Prot P25338 (YGB0_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized endoplasmic reticulum membrane protein YGL010W

Gene names

Ordered Locus Names:	YGL010W
ORF Names:	YGL021

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Not an essential protein for cell growth.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Miscellaneous	Present with 486 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	To S.pombe SpAC16E8.02.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Ref.4 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPF1	P39986	1	EBI-23699,EBI-3128

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	Uncharacterized endoplasmic reticulum membrane protein YGL010W		PRO_0000202776
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Potential
	Topological domain	45 – 63	19	Lumenal Potential
	Transmembrane	64 – 84	21	Potential
	Topological domain	85 – 98	14	Cytoplasmic Potential
	Transmembrane	99 – 119	21	Potential
	Topological domain	120 – 131	12	Lumenal Potential
	Transmembrane	132 – 152	21	Potential
	Topological domain	153 – 174	22	Cytoplasmic Potential
Experimental info
	Sequence conflict	105	1	T → I Ref.1
	Sequence conflict	105	1	T → I Ref.2
	Sequence conflict	122 – 174	53	RPALI…RMQRQ → TSQR Ref.1
	Sequence conflict	122 – 174	53	RPALI…RMQRQ → TSQR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P25338-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: C6C5DF3C6EDBD3B1	FASTA	174	20,173
	10 20 30 40 50 60 MGEGLLDLRS QLGFYKFYHH NPKNVLIHSI FVPTILFSGS CMLHRVKIYQ SISLTAVLSV 70 80 90 100 110 120 LFSIFYCLLY LPTGLLAGVL LLLLNLALID HRVDLTFKQE LGLFTIGWIF QFVGHGVFEK 130 140 150 160 170 RRPALIDNLV QSLVLAPYFI MFEFLFKLGF MPRLKATLEH DLEIKQRNLR MQRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The YGL021 gene encodes a putative membrane protein with a putative leucine zipper motif." Chen W., Capieaux E., Balzi E., Goffeau A. Yeast 7:301-303(1991) [PubMed: 1882554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 46191 / IL125-2B.
[2]	"The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance." Chen W., Balzi E., Capieaux E., Choder M., Goffeau A. Yeast 7:287-299(1991) [PubMed: 1882553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 46191 / IL125-2B.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S58126 Genomic DNA. Translation: AAD13893.1. S57893 Genomic DNA. Translation: AAB19614.1. Z72532 Genomic DNA. Translation: CAA96710.1.
PIR	S64012.
RefSeq	NP_011505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4727N.
IntAct	P25338. 3 interactions.
STRING	P25338.
Genome annotation databases
Ensembl	YGL010W; YGL010W; YGL010W; Saccharomyces cerevisiae. [Genome view]
GeneID	852874.
KEGG	sce:YGL010W.
NMPDR	fig|4932.3.peg.2613.
Organism-specific databases
CYGD	YGL010w.
SGD	S000002978. YGL010W.
Phylogenomic databases
HOGENOM	P25338.
OMA	QAFLMAP
OrthoDB	EOG9HMKSS
Gene expression databases
ArrayExpress	P25338.
Genevestigator	P25338.
GermOnline	YGL010W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009305. DUF962. [Graphical view]
Pfam	PF06127. DUF962. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972508.

Entry information

Entry name

YGB0_YEAST

Accession

Primary (citable) accession number: P25338

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)