P25335 (ALLC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Allantoicase
EC=3.5.3.4

Alternative name(s):
Allantoate amidinohydrolase

Gene names

Name:	DAL2
Synonyms:	ALC1
Ordered Locus Names:	YIR029W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Utilization of purines as secondary nitrogen sources, when primary sources are limiting.
Catalytic activity	Allantoate + H2O = (S)-ureidoglycolate + urea.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; (S)-ureidoglycolate from allantoate (aminidohydrolase route): step 1/1.
Induction	Repressed by nitrogen.
Sequence similarities	Belongs to the allantoicase family.

Ontologies

Keywords
Biological process	Purine metabolism
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	allantoin catabolic process Inferred from mutant phenotype Ref.1. Source: SGD purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	allantoicase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BOI1	P38041	1	EBI-2442,EBI-3719

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 343

343

Allantoicase

PRO_0000205914

Experimental info

Sequence conflict

93

1

A → S in CAA42994. Ref.2

Sequence conflict

134 – 135

2

WV → SL in CAA42994. Ref.2

Secondary structure

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343

Helix Strand Turn

Details...

Beta strand

2 – 4

3

Helix

6 – 8

3

Helix

9 – 16

8

Turn

17 – 20

4

Helix

28 – 30

3

Beta strand

33 – 38

6

Beta strand

41 – 43

3

Helix

45 – 49

5

Beta strand

69 – 72

4

Beta strand

78 – 80

3

Beta strand

82 – 87

6

Beta strand

89 – 91

3

Beta strand

93 – 101

9

Beta strand

106 – 109

4

Beta strand

111 – 118

8

Beta strand

135 – 142

8

Beta strand

148 – 152

5

Beta strand

162 – 171

10

Beta strand

174 – 184

11

Helix

201 – 203

3

Beta strand

206 – 211

6

Beta strand

214 – 216

3

Helix

218 – 222

5

Beta strand

224 – 226

3

Helix

230 – 232

3

Beta strand

233 – 235

3

Beta strand

246 – 251

6

Beta strand

257 – 265

9

Beta strand

270 – 272

3

Beta strand

275 – 282

8

Beta strand

297 – 305

9

Beta strand

311 – 319

9

Beta strand

323 – 331

9

Beta strand

337 – 343

7

Sequences

	Sequence		Length	Mass (Da)
	P25335 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 0F9CB0FBA5EB76F1	FASTA	343	38,714
	10 20 30 40 50 60 MKFFSLADEA EFKSIIISKN KAVDVIGSKL GGQVVSFSDE WFASAENLIQ PTAPIRDPTR 70 80 90 100 110 120 FVHSGAWYDG WETRRHNEME YDWVIIKMGV AAAHIIGGEI DTAFFNGNHA PFVSIEALYD 130 140 150 160 170 180 EGEEGNIVED DSRWVEIVEK FECGPSQRHL FVRGNGLTKE RFTHIKLKMY PDGGIARFRL 190 200 210 220 230 240 YGRVVPPELK TKDHIIDLAY VCNGAVALKY SDQHFGSVDN LLLPGRGHDM SDGWETKRSR 250 260 270 280 290 300 QPGHTDWAVI QLGRESSFIE KIIVDTAHFR GNFPQFITVE GCLKESESSE NTGEGTWVEL 310 320 330 340 VGKSKTGPDK EHVYEIRKSI RVSHVKLTII PDGGVKRIRV WGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequences of two adjacent genes, one (DAL2) encoding allantoicase and another (DCG1) sensitive to nitrogen-catabolite repression in Saccharomyces cerevisiae." Yoo H.S., Cooper T.G. Gene 104:55-62(1991) [PubMed: 1916277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of a Saccharomyces cerevisiae gene encoding a protein homologous to allantoicase of Neurospora crassa." Lee F.-J.S., Moss J. Yeast 7:993-995(1991) [PubMed: 1839481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Crystal structure of yeast allantoicase reveals a repeated jelly roll motif." Leulliot N., Quevillon-Cheruel S., Sorel I., Graille M., Meyer P., Liger D., Blondeau K., Janin J., van Tilbeurgh H. J. Biol. Chem. 279:23447-23452(2004) [PubMed: 15020593] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
[6]	"Crystal structure of an allantoicase (YIR029W) from Saccharomyces cerevisiae at 2.4 A resolution." Xu Q., Schwarzenbacher R., Page R., Sims E., Abdubek P., Ambing E., Biorac T., Brinen L.S., Cambell J., Canaves J.M., Chiu H.J., Dai X., Deacon A.M., DiDonato M., Elsliger M.-A., Floyd R., Godzik A., Grittini C. , Grzechnik S.K., Hampton E., Jaroszewski L., Karlak C., Klock H.E., Koesema E., Kovarik J.S., Kreusch A., Kuhn P., Lesley S.A., Levin I., McMullan D., McPhillips T.M., Miller M.D., Morse A., Moy K., Ouyang J., Quijano K., Reyes R., Rezezadeh F., Robb A., Spraggon G., Stevens R.C., van den Bedem H., Velasquez J., Vincent J., von Delft F., Wang X., West B., Wolf G., Hodgson K.O., Wooley J., Wilson I.A. Proteins 56:619-624(2004) [PubMed: 15229895] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M64720 Genomic DNA. Translation: AAA34554.1.
X60460 Genomic DNA. Translation: CAA42994.1.
Z38061 Genomic DNA. Translation: CAA86189.1.
BK006942 Genomic DNA. Translation: DAA08576.1.

PIR

JH0442.

RefSeq

NP_012295.1. NM_001179551.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O59	X-ray	2.40	A	1-343	[»]
1SG3	X-ray	2.60	A/B	1-343	[»]

ProteinModelPortal

P25335.

SMR

P25335. Positions 1-343.

ModBase

Search...

Protein-protein interaction databases

IntAct

P25335. 1 interaction.

MINT

MINT-1761898.

STRING

P25335.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YIR029W; YIR029W; YIR029W.

GeneID

854847.

KEGG

sce:YIR029W.

NMPDR

fig|4932.3.peg.1838.

Organism-specific databases

CYGD

YIR029w.

SGD

S000001468. DAL2.

Phylogenomic databases

eggNOG

fuNOG06867.

GeneTree

EFGT00050000007906.

HOGENOM

HBG480577.

OMA

NSHHYHE.

OrthoDB

EOG4V9Z0N.

Enzyme and pathway databases

BioCyc

MetaCyc:YIR029W-MONOMER.

Gene expression databases

ArrayExpress

P25335.

Genevestigator

P25335.

GermOnline

YIR029W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005164. Allantoicase.
IPR015908. Allantoicase_dom.
IPR008979. Galactose-bd-like.
[Graphical view]

KO

K01477.

PANTHER

PTHR12045. Allantoicase. 1 hit.

Pfam

PF03561. Allantoicase. 2 hits.
[Graphical view]

PIRSF

PIRSF016516. Allantoicase. 1 hit.

SUPFAM

SSF49785. Gal_bind_like. 2 hits.

TIGRFAMs

TIGR02961. Allantoicase. 1 hit.

ProtoNet

Search...

Other

NextBio

977740.

Entry information

Entry name

ALLC_YEAST

Accession

Primary (citable) accession number: P25335
Secondary accession number(s): D6VVW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	December 14, 2011
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program