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P25333

- HAL4_YEAST

UniProt

P25333 - HAL4_YEAST

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Protein

Serine/threonine-protein kinase HAL4/SAT4

Gene

SAT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Promotes K+ uptake, by the potassium transporter TRK1-TRK2, which leads to the subsequent cellular resistance to toxic cations such as Na+, Li+ and Ca2+.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei353 – 3531ATPPROSITE-ProRule annotation
Active sitei449 – 4491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi322 – 3309ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase activity Source: SGD
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular cation homeostasis Source: SGD
  2. cellular protein localization Source: SGD
  3. G1/S transition of mitotic cell cycle Source: SGD
  4. protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29325-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase HAL4/SAT4 (EC:2.7.11.1)
Alternative name(s):
Halotolerance protein 4
Gene namesi
Name:SAT4
Synonyms:HAL4
Ordered Locus Names:YCR008W
ORF Names:YCR046, YCR101, YCR8W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR008w.
SGDiS000000601. SAT4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 603603Serine/threonine-protein kinase HAL4/SAT4PRO_0000085987Add
BLAST

Proteomic databases

MaxQBiP25333.
PaxDbiP25333.

Expressioni

Gene expression databases

GenevestigatoriP25333.

Interactioni

Protein-protein interaction databases

BioGridi30987. 148 interactions.
DIPiDIP-4495N.
IntActiP25333. 1 interaction.
MINTiMINT-571030.
STRINGi4932.YCR008W.

Structurei

3D structure databases

ProteinModelPortaliP25333.
SMRiP25333. Positions 236-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini316 – 590275Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi99 – 11113Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000075309.
HOGENOMiHOG000172097.
InParanoidiP25333.
KOiK08286.
OMAiPRCVDIW.
OrthoDBiEOG7008J9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25333-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGMNDNNAA IPQQTPRKHA LSSKVMQLFR SGSRSSRQGK ASSNIQPPSN
60 70 80 90 100
INTNVPSASK SAKFGLHTPT TATPRVVSNP SNTAGVSKPG MYMPEYYQSA
110 120 130 140 150
SPSHSSSSAS LNNHIDINTS KSSSAASLTS SVSALSLSPT SAINISSKSL
160 170 180 190 200
SPKFSHHSNS NTAITPAPTP TASNINNVNK ITNTSAPICG RFLVHKDGTH
210 220 230 240 250
EHHLKNAKRQ EKLSTMIKNM VGASKLRGEA KSAVPDIIMD PKTTLKSNKN
260 270 280 290 300
PPTLFAGFMK QVVDMDDKYP EGAPTSGALN CPERDIYRSD QKDSKNNTHN
310 320 330 340 350
ITTTKKDRQC FAEKYGRCQE VLGKGAFGVV RICQKKNVSS QDGNKSEKLY
360 370 380 390 400
AVKEFKRRTS ESAEKYSKRL TSEFCISSSL HHTNIVTTLD LFQDAKGEYC
410 420 430 440 450
EVMEYCAGGD LFTLVVAAGK LEYMEADCFF KQLIRGVVYM HEMGVCHRDL
460 470 480 490 500
KPENLLLTHD GVLKITDFGN SECFKMAWEK NIHLSGGVCG SSPYIAPEEY
510 520 530 540 550
IKEEFDPRPV DIWACGVIYM AMRTGRQLWS SAEKDDPFYM NYLKGRKEKG
560 570 580 590 600
GYEPIESLKR ARCRNVIYSM LDPVPYRRIN GKQILNSEWG REIKCCHNGR

ALK
Length:603
Mass (Da):66,666
Last modified:May 1, 1992 - v1
Checksum:i9DD31B74C05EE212
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76380 Genomic DNA. Translation: AAB20894.1.
X59720 Genomic DNA. Translation: CAA42325.1.
Z11114 Genomic DNA. Translation: CAA77445.1.
BK006937 Genomic DNA. Translation: DAA07486.1.
PIRiS17470. OKBY8W.
RefSeqiNP_009934.1. NM_001178721.1.

Genome annotation databases

EnsemblFungiiYCR008W; YCR008W; YCR008W.
GeneIDi850366.
KEGGisce:YCR008W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76380 Genomic DNA. Translation: AAB20894.1 .
X59720 Genomic DNA. Translation: CAA42325.1 .
Z11114 Genomic DNA. Translation: CAA77445.1 .
BK006937 Genomic DNA. Translation: DAA07486.1 .
PIRi S17470. OKBY8W.
RefSeqi NP_009934.1. NM_001178721.1.

3D structure databases

ProteinModelPortali P25333.
SMRi P25333. Positions 236-589.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30987. 148 interactions.
DIPi DIP-4495N.
IntActi P25333. 1 interaction.
MINTi MINT-571030.
STRINGi 4932.YCR008W.

Proteomic databases

MaxQBi P25333.
PaxDbi P25333.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR008W ; YCR008W ; YCR008W .
GeneIDi 850366.
KEGGi sce:YCR008W.

Organism-specific databases

CYGDi YCR008w.
SGDi S000000601. SAT4.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000075309.
HOGENOMi HOG000172097.
InParanoidi P25333.
KOi K08286.
OMAi PRCVDIW.
OrthoDBi EOG7008J9.

Enzyme and pathway databases

BioCyci YEAST:G3O-29325-MONOMER.
BRENDAi 2.7.11.1. 984.

Miscellaneous databases

NextBioi 965849.
PROi P25333.

Gene expression databases

Genevestigatori P25333.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The open reading frame YCR101 located on chromosome III from Saccharomyces cerevisiae is a putative protein kinase."
    Skala J., Purnelle B., Crouzet M., Aigle M., Goffeau A.
    Yeast 7:651-655(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The complete sequence of a 10.8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae."
    Biteau N., Fremaux C., Hebrard S., Menara A., Aigle M., Crouzet M.
    Yeast 8:61-70(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-240.
  5. "A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter."
    Mulet J.M., Leube M.P., Kron S.J., Rios G., Fink G.R., Serrano R.
    Mol. Cell. Biol. 19:3328-3337(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHAL4_YEAST
AccessioniPrimary (citable) accession number: P25333
Secondary accession number(s): D6VR17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1030 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3