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P25333 (HAL4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase HAL4/SAT4

EC=2.7.11.1
Alternative name(s):
Halotolerance protein 4
Gene names
Name:SAT4
Synonyms:HAL4
Ordered Locus Names:YCR008W
ORF Names:YCR046, YCR101, YCR8W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length603 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes K+ uptake, by the potassium transporter TRK1-TRK2, which leads to the subsequent cellular resistance to toxic cations such as Na+, Li+ and Ca2+. Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Miscellaneous

Present with 1030 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 603603Serine/threonine-protein kinase HAL4/SAT4
PRO_0000085987

Regions

Domain316 – 590275Protein kinase
Nucleotide binding322 – 3309ATP By similarity
Compositional bias99 – 11113Ser-rich

Sites

Active site4491Proton acceptor By similarity
Binding site3531ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P25333 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 9DD31B74C05EE212

FASTA60366,666
        10         20         30         40         50         60 
MTGMNDNNAA IPQQTPRKHA LSSKVMQLFR SGSRSSRQGK ASSNIQPPSN INTNVPSASK 

        70         80         90        100        110        120 
SAKFGLHTPT TATPRVVSNP SNTAGVSKPG MYMPEYYQSA SPSHSSSSAS LNNHIDINTS 

       130        140        150        160        170        180 
KSSSAASLTS SVSALSLSPT SAINISSKSL SPKFSHHSNS NTAITPAPTP TASNINNVNK 

       190        200        210        220        230        240 
ITNTSAPICG RFLVHKDGTH EHHLKNAKRQ EKLSTMIKNM VGASKLRGEA KSAVPDIIMD 

       250        260        270        280        290        300 
PKTTLKSNKN PPTLFAGFMK QVVDMDDKYP EGAPTSGALN CPERDIYRSD QKDSKNNTHN 

       310        320        330        340        350        360 
ITTTKKDRQC FAEKYGRCQE VLGKGAFGVV RICQKKNVSS QDGNKSEKLY AVKEFKRRTS 

       370        380        390        400        410        420 
ESAEKYSKRL TSEFCISSSL HHTNIVTTLD LFQDAKGEYC EVMEYCAGGD LFTLVVAAGK 

       430        440        450        460        470        480 
LEYMEADCFF KQLIRGVVYM HEMGVCHRDL KPENLLLTHD GVLKITDFGN SECFKMAWEK 

       490        500        510        520        530        540 
NIHLSGGVCG SSPYIAPEEY IKEEFDPRPV DIWACGVIYM AMRTGRQLWS SAEKDDPFYM 

       550        560        570        580        590        600 
NYLKGRKEKG GYEPIESLKR ARCRNVIYSM LDPVPYRRIN GKQILNSEWG REIKCCHNGR 


ALK 

« Hide

References

« Hide 'large scale' references
[1]"The open reading frame YCR101 located on chromosome III from Saccharomyces cerevisiae is a putative protein kinase."
Skala J., Purnelle B., Crouzet M., Aigle M., Goffeau A.
Yeast 7:651-655(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The complete sequence of a 10.8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae."
Biteau N., Fremaux C., Hebrard S., Menara A., Aigle M., Crouzet M.
Yeast 8:61-70(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-240.
[5]"A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter."
Mulet J.M., Leube M.P., Kron S.J., Rios G., Fink G.R., Serrano R.
Mol. Cell. Biol. 19:3328-3337(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S76380 Genomic DNA. Translation: AAB20894.1.
X59720 Genomic DNA. Translation: CAA42325.1.
Z11114 Genomic DNA. Translation: CAA77445.1.
BK006937 Genomic DNA. Translation: DAA07486.1.
PIROKBY8W. S17470.
RefSeqNP_009934.1. NM_001178721.1.

3D structure databases

ProteinModelPortalP25333.
SMRP25333. Positions 236-589.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid30987. 148 interactions.
DIPDIP-4495N.
IntActP25333. 1 interaction.
MINTMINT-571030.
STRING4932.YCR008W.

Proteomic databases

MaxQBP25333.
PaxDbP25333.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR008W; YCR008W; YCR008W.
GeneID850366.
KEGGsce:YCR008W.

Organism-specific databases

CYGDYCR008w.
SGDS000000601. SAT4.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000075309.
HOGENOMHOG000172097.
KOK08286.
OMAPRCVDIW.
OrthoDBEOG7008J9.

Enzyme and pathway databases

BioCycYEAST:G3O-29325-MONOMER.
BRENDA2.7.11.1. 984.

Gene expression databases

GenevestigatorP25333.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965849.
PROP25333.

Entry information

Entry nameHAL4_YEAST
AccessionPrimary (citable) accession number: P25333
Secondary accession number(s): D6VR17
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 14, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families