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P25333

- HAL4_YEAST

UniProt

P25333 - HAL4_YEAST

Protein

Serine/threonine-protein kinase HAL4/SAT4

Gene

SAT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Promotes K+ uptake, by the potassium transporter TRK1-TRK2, which leads to the subsequent cellular resistance to toxic cations such as Na+, Li+ and Ca2+.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei353 – 3531ATPPROSITE-ProRule annotation
    Active sitei449 – 4491Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi322 – 3309ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: SGD
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular cation homeostasis Source: SGD
    2. cellular protein localization Source: SGD
    3. G1/S transition of mitotic cell cycle Source: SGD
    4. protein dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29325-MONOMER.
    BRENDAi2.7.11.1. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase HAL4/SAT4 (EC:2.7.11.1)
    Alternative name(s):
    Halotolerance protein 4
    Gene namesi
    Name:SAT4
    Synonyms:HAL4
    Ordered Locus Names:YCR008W
    ORF Names:YCR046, YCR101, YCR8W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR008w.
    SGDiS000000601. SAT4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 603603Serine/threonine-protein kinase HAL4/SAT4PRO_0000085987Add
    BLAST

    Proteomic databases

    MaxQBiP25333.
    PaxDbiP25333.

    Expressioni

    Gene expression databases

    GenevestigatoriP25333.

    Interactioni

    Protein-protein interaction databases

    BioGridi30987. 148 interactions.
    DIPiDIP-4495N.
    IntActiP25333. 1 interaction.
    MINTiMINT-571030.
    STRINGi4932.YCR008W.

    Structurei

    3D structure databases

    ProteinModelPortaliP25333.
    SMRiP25333. Positions 236-589.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini316 – 590275Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi99 – 11113Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000075309.
    HOGENOMiHOG000172097.
    KOiK08286.
    OMAiPRCVDIW.
    OrthoDBiEOG7008J9.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25333-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGMNDNNAA IPQQTPRKHA LSSKVMQLFR SGSRSSRQGK ASSNIQPPSN    50
    INTNVPSASK SAKFGLHTPT TATPRVVSNP SNTAGVSKPG MYMPEYYQSA 100
    SPSHSSSSAS LNNHIDINTS KSSSAASLTS SVSALSLSPT SAINISSKSL 150
    SPKFSHHSNS NTAITPAPTP TASNINNVNK ITNTSAPICG RFLVHKDGTH 200
    EHHLKNAKRQ EKLSTMIKNM VGASKLRGEA KSAVPDIIMD PKTTLKSNKN 250
    PPTLFAGFMK QVVDMDDKYP EGAPTSGALN CPERDIYRSD QKDSKNNTHN 300
    ITTTKKDRQC FAEKYGRCQE VLGKGAFGVV RICQKKNVSS QDGNKSEKLY 350
    AVKEFKRRTS ESAEKYSKRL TSEFCISSSL HHTNIVTTLD LFQDAKGEYC 400
    EVMEYCAGGD LFTLVVAAGK LEYMEADCFF KQLIRGVVYM HEMGVCHRDL 450
    KPENLLLTHD GVLKITDFGN SECFKMAWEK NIHLSGGVCG SSPYIAPEEY 500
    IKEEFDPRPV DIWACGVIYM AMRTGRQLWS SAEKDDPFYM NYLKGRKEKG 550
    GYEPIESLKR ARCRNVIYSM LDPVPYRRIN GKQILNSEWG REIKCCHNGR 600
    ALK 603
    Length:603
    Mass (Da):66,666
    Last modified:May 1, 1992 - v1
    Checksum:i9DD31B74C05EE212
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S76380 Genomic DNA. Translation: AAB20894.1.
    X59720 Genomic DNA. Translation: CAA42325.1.
    Z11114 Genomic DNA. Translation: CAA77445.1.
    BK006937 Genomic DNA. Translation: DAA07486.1.
    PIRiS17470. OKBY8W.
    RefSeqiNP_009934.1. NM_001178721.1.

    Genome annotation databases

    EnsemblFungiiYCR008W; YCR008W; YCR008W.
    GeneIDi850366.
    KEGGisce:YCR008W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S76380 Genomic DNA. Translation: AAB20894.1 .
    X59720 Genomic DNA. Translation: CAA42325.1 .
    Z11114 Genomic DNA. Translation: CAA77445.1 .
    BK006937 Genomic DNA. Translation: DAA07486.1 .
    PIRi S17470. OKBY8W.
    RefSeqi NP_009934.1. NM_001178721.1.

    3D structure databases

    ProteinModelPortali P25333.
    SMRi P25333. Positions 236-589.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30987. 148 interactions.
    DIPi DIP-4495N.
    IntActi P25333. 1 interaction.
    MINTi MINT-571030.
    STRINGi 4932.YCR008W.

    Proteomic databases

    MaxQBi P25333.
    PaxDbi P25333.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR008W ; YCR008W ; YCR008W .
    GeneIDi 850366.
    KEGGi sce:YCR008W.

    Organism-specific databases

    CYGDi YCR008w.
    SGDi S000000601. SAT4.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000075309.
    HOGENOMi HOG000172097.
    KOi K08286.
    OMAi PRCVDIW.
    OrthoDBi EOG7008J9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29325-MONOMER.
    BRENDAi 2.7.11.1. 984.

    Miscellaneous databases

    NextBioi 965849.
    PROi P25333.

    Gene expression databases

    Genevestigatori P25333.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The open reading frame YCR101 located on chromosome III from Saccharomyces cerevisiae is a putative protein kinase."
      Skala J., Purnelle B., Crouzet M., Aigle M., Goffeau A.
      Yeast 7:651-655(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The complete sequence of a 10.8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae."
      Biteau N., Fremaux C., Hebrard S., Menara A., Aigle M., Crouzet M.
      Yeast 8:61-70(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-240.
    5. "A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter."
      Mulet J.M., Leube M.P., Kron S.J., Rios G., Fink G.R., Serrano R.
      Mol. Cell. Biol. 19:3328-3337(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHAL4_YEAST
    AccessioniPrimary (citable) accession number: P25333
    Secondary accession number(s): D6VR17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1030 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3