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P25332 (RBSK_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ribokinase
EC=2.7.1.15
Gene names
Name:RBK1
Ordered Locus Names:YCR036W
ORF Names:YCR36W, YCR523
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + D-ribose = ADP + D-ribose 5-phosphate.

Pathway

Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.

Subcellular location

Cytoplasm. Nucleus Ref.5.

Miscellaneous

Present with 1780 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the carbohydrate kinase PfkB family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PEX14P531121EBI-14854,EBI-13212
SSA1P105911EBI-14854,EBI-8591

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Probable ribokinase
PRO_0000080094

Experimental info

Sequence conflict1781A → T in CAA40228. Ref.1
Sequence conflict3011T → M in CAA40228. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25332 [UniParc].

Last modified March 15, 2004. Version 2.
Checksum: 226B44DA7E458B95

FASTA33336,924
        10         20         30         40         50         60 
MGITVIGSLN YDLDTFTDRL PNAGETFRAN HFETHAGGKG LNQAAAIGKL KNPSSRYSVR 

        70         80         90        100        110        120 
MIGNVGNDTF GKQLKDTLSD CGVDITHVGT YEGINTGTAT ILIEEKAGGQ NRILIVEGAN 

       130        140        150        160        170        180 
SKTIYDPKQL CEIFPEGKEE EEYVVFQHEI PDPLSIIKWI HANRPNFQIV YNPSPFKAMP 

       190        200        210        220        230        240 
KKDWELVDLL VVNEIEGLQI VESVFDNELV EEIREKIKDD FLGEYRKICE LLYEKLMNRK 

       250        260        270        280        290        300 
KRGIVVMTLG SRGVLFCSHE SPEVQFLPAI QNVSVVDTTG AGDTFLGGLV TQLYQGETLS 

       310        320        330 
TAIKFSTLAS SLTIQRKGAA ESMPLYKDVQ KDA

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase."
Thierry A., Fairhead C., Dujon B.
Yeast 6:521-534(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 178 AND 301.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X56909 Genomic DNA. Translation: CAA40228.1.
X59720 Genomic DNA. Translation: CAA42303.2.
BK006937 Genomic DNA. Translation: DAA07515.1.
PIRKIBYRB. S12918.
RefSeqNP_009965.2. NM_001178750.1.

3D structure databases

ProteinModelPortalP25332.
SMRP25332. Positions 2-329.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4990N.
IntActP25332. 2 interactions.
MINTMINT-551300.
STRING4932.YCR036W.

Proteomic databases

PaxDbP25332.
PeptideAtlasP25332.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR036W; YCR036W; YCR036W.
GeneID850402.
KEGGsce:YCR036W.

Organism-specific databases

CYGDYCR036w.
SGDS000000632. RBK1.

Phylogenomic databases

eggNOGCOG0524.
GeneTreeENSGT00390000005743.
HOGENOMHOG000235950.
KOK00852.
OMANEIPHQA.
OrthoDBEOG4QJVX3.

Enzyme and pathway databases

UniPathwayUPA00916; UER00889.

Gene expression databases

GenevestigatorP25332.
GermOnlineYCR036W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR002139. Ribokinase.
[Graphical view]
PfamPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSPR00990. RIBOKINASE.
PROSITEPS00583. PFKB_KINASES_1. False negative.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965941.

Entry information

Entry nameRBSK_YEAST
AccessionPrimary (citable) accession number: P25332
Secondary accession number(s): D6VR46
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: March 15, 2004
Last modified: May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents