P25332 (RBSK_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ribokinase
EC=2.7.1.15

Gene names

Name:	RBK1
Ordered Locus Names:	YCR036W
ORF Names:	YCR36W, YCR523

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-ribose = ADP + D-ribose 5-phosphate.
Pathway	Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 1780 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	D-ribose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEX14	P53112	1	EBI-14854,EBI-13212
SSA1	P10591	1	EBI-14854,EBI-8591

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Probable ribokinase		PRO_0000080094
Experimental info
	Sequence conflict	178	1	A → T in CAA40228. Ref.1
	Sequence conflict	301	1	T → M in CAA40228. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25332 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: 226B44DA7E458B95	FASTA	333	36,924
	10 20 30 40 50 60 MGITVIGSLN YDLDTFTDRL PNAGETFRAN HFETHAGGKG LNQAAAIGKL KNPSSRYSVR 70 80 90 100 110 120 MIGNVGNDTF GKQLKDTLSD CGVDITHVGT YEGINTGTAT ILIEEKAGGQ NRILIVEGAN 130 140 150 160 170 180 SKTIYDPKQL CEIFPEGKEE EEYVVFQHEI PDPLSIIKWI HANRPNFQIV YNPSPFKAMP 190 200 210 220 230 240 KKDWELVDLL VVNEIEGLQI VESVFDNELV EEIREKIKDD FLGEYRKICE LLYEKLMNRK 250 260 270 280 290 300 KRGIVVMTLG SRGVLFCSHE SPEVQFLPAI QNVSVVDTTG AGDTFLGGLV TQLYQGETLS 310 320 330 TAIKFSTLAS SLTIQRKGAA ESMPLYKDVQ KDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase." Thierry A., Fairhead C., Dujon B. Yeast 6:521-534(1990) [PubMed: 1964349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Valles G., Volckaerts G. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 178 AND 301.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56909 Genomic DNA. Translation: CAA40228.1. X59720 Genomic DNA. Translation: CAA42303.2. BK006937 Genomic DNA. Translation: DAA07515.1.
PIR	KIBYRB. S12918.
RefSeq	NP_009965.2. NM_001178750.1.
3D structure databases
ProteinModelPortal	P25332.
SMR	P25332. Positions 1-332.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4990N.
IntAct	P25332. 2 interactions.
MINT	MINT-551300.
STRING	P25332.
Proteomic databases
PeptideAtlas	P25332.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR036W; YCR036W; YCR036W.
GeneID	850402.
KEGG	sce:YCR036W.
NMPDR	fig|4932.3.peg.690.
Organism-specific databases
CYGD	YCR036w.
SGD	S000000632. RBK1.
Phylogenomic databases
eggNOG	fuNOG05396.
GeneTree	EFGT00050000002259.
HOGENOM	HBG697973.
OMA	LLIMQLE.
OrthoDB	EOG4QJVX3.
Gene expression databases
ArrayExpress	P25332.
Genevestigator	P25332.
GermOnline	YCR036W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. IPR002139. Ribokinase. [Graphical view]
KO	K00852.
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PRINTS	PR00990. RIBOKINASE.
PROSITE	PS00583. PFKB_KINASES_1. False negative. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	965941.

Entry information

Entry name

RBSK_YEAST

Accession

Primary (citable) accession number: P25332
Secondary accession number(s): D6VR46

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	March 15, 2004
Last modified:	December 14, 2011
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program