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P25318 (CO8A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Collagen alpha-2(VIII) chain
Alternative name(s):
Endothelial collagen
Gene names
Name:Col8a2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length699 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis By similarity. Ref.5

Subunit structure

Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrhedron stabilized by central interacting C-terminal NC1 trimers By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane.

Tissue specificity

In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Ref.5

Post-translational modification

Proteolytically cleaved by neutrophil elastase, in vitro By similarity.

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Disruption phenotype

COL8A1-/COL8A2- mice exhibit decreased proliferation of mesangial cells, reduced phosphorylation of ERK1/2 and increased p27(KIP1) expression. Diabetic COL8A1-/COL8A2- mice reveal reduced mesangial expansion and cellularity and extracellular matrix expansion. Ref.5

Sequence similarities

Contains 1 C1q domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P25318-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P25318-2)

The sequence of this isoform differs from the canonical sequence as follows:
     168-223: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 699675Collagen alpha-2(VIII) chain
PRO_0000005836

Regions

Domain566 – 699134C1q
Region25 – 7248Nonhelical region (NC2)
Region73 – 532460Triple-helical region (COL1)
Region533 – 699167Nonhelical region (NC1)
Compositional bias17 – 563547Gly-rich

Natural variations

Alternative sequence168 – 22356Missing in isoform 2.
VSP_014975

Experimental info

Sequence conflict6091T → S in M60833. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 16, 2005. Version 2.
Checksum: FCBD4FBB44642646

FASTA69966,943
        10         20         30         40         50         60 
MQGALMPLPS LLLLLLGCGP RVSSGGGAGG AAGYAPVKYV QPMQKGPVGP PFREGKGQYL 

        70         80         90        100        110        120 
EMPLPMLPMD LKGEPGPPGK PGPRGPPGPP GFPGKPGTGK PGVHGQPGPA GPPGFSRMGK 

       130        140        150        160        170        180 
AGPPGLPGKV GPPGQPGLRG EPGIRGDQGL RGPPGPPGLP GPSGITVPGK PGAQGAPGPP 

       190        200        210        220        230        240 
GFRGEPGPQG EPGPRGDRGL KGDNGVGQPG LPGAPGQAGA PGPPGLPGPA GLGKPGLDGI 

       250        260        270        280        290        300 
PGAPGDKGDS GPPGVPGSRG EPGAVGPKGP PGVDGVGIPG AAGVPGPQGP VGAKGEPGLR 

       310        320        330        340        350        360 
GPPGLIGPVG YGMPGKPGPK GDRGPVGAPG LLGDRGEPGE DGKPGEQGPQ GLGGPPGLPG 

       370        380        390        400        410        420 
SAGLPGRRGP PGSKGEVGPG GPPGVPGIRG DQGPNGLAGK PGLPGERGLP GAHGPPGPTG 

       430        440        450        460        470        480 
PKGEPGFTGR PGGPGVAGAL GQKGDLGLPG QPGLRGPSGI PGLQGPAGPI GPQGLPGLKG 

       490        500        510        520        530        540 
EPGLPGPPGE GKVGEPGSAG PTGPPGVPGS PGLTGPPGPP GPPGPPGAPG ALDETGIAGL 

       550        560        570        580        590        600 
HLPNGGVEGA VLGKGGKPQF GLGELSAHAT PAFTAVLTSP FPASGMPVRF DRTLYNGHSG 

       610        620        630        640        650        660 
YNPATGIFTC PVGGVYYFAY HVHVKGTNVW VALYKNNVPA TYTYDEYKKG YLDQASGGAV 

       670        680        690 
LQLRPNDQVW VQMPSDQANG LYSTEYIHSS FSGFLLCPT 

« Hide

Isoform 2 [UniParc].

Checksum: FE19B6EC5AAB2A8B
Show »

FASTA64361,831

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Brain.
[3]"Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-699 (ISOFORM 1).
Tissue: Embryonic tail.
[4]"The alpha 2(VIII) collagen gene. A novel member of the short chain collagen family located on the human chromosome 1."
Muragaki Y., Jacenko O., Apte S., Mattei M.-G., Ninomiya Y., Olsen B.R.
J. Biol. Chem. 266:7721-7727(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 530-699.
[5]"Lack of type VIII collagen in mice ameliorates diabetic nephropathy."
Hopfer U., Hopfer H., Meyer-Schwesinger C., Loeffler I., Fukai N., Olsen B.R., Stahl R.A., Wolf G.
Diabetes 58:1672-1681(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL606976 Genomic DNA. Translation: CAM45876.1.
BC065148 mRNA. Translation: AAH65148.1.
BC080317 mRNA. Translation: AAH80317.1.
AK131153 mRNA. Translation: BAD21403.1.
M60833 Genomic DNA. No translation available.
CCDSCCDS18649.1. [P25318-1]
PIRB57131.
RefSeqNP_955767.1. NM_199473.2. [P25318-1]
UniGeneMm.296327.

3D structure databases

ProteinModelPortalP25318.
SMRP25318. Positions 571-698.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP25318.

Proteomic databases

PRIDEP25318.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070132; ENSMUSP00000070270; ENSMUSG00000056174. [P25318-1]
GeneID329941.
KEGGmmu:329941.
UCSCuc008utd.1. mouse. [P25318-1]

Organism-specific databases

CTD1296.
MGIMGI:88464. Col8a2.

Phylogenomic databases

eggNOGNOG114228.
GeneTreeENSGT00750000117378.
HOGENOMHOG000085653.
HOVERGENHBG108220.
InParanoidA3KFY2.
OMAAGPPFRE.
OrthoDBEOG70ZZPW.
PhylomeDBP25318.
TreeFamTF334029.

Gene expression databases

ArrayExpressP25318.
BgeeP25318.
CleanExMM_COL8A2.
GenevestigatorP25318.

Family and domain databases

Gene3D2.60.120.40. 1 hit.
InterProIPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamPF00386. C1q. 1 hit.
PF01391. Collagen. 2 hits.
[Graphical view]
PRINTSPR00007. COMPLEMNTC1Q.
SMARTSM00110. C1Q. 1 hit.
[Graphical view]
SUPFAMSSF49842. SSF49842. 1 hit.
PROSITEPS50871. C1Q. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio399063.
PROP25318.
SOURCESearch...

Entry information

Entry nameCO8A2_MOUSE
AccessionPrimary (citable) accession number: P25318
Secondary accession number(s): A3KFY2 expand/collapse secondary AC list , Q68ED0, Q6KAQ4, Q6P1C4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: August 16, 2005
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot