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P25318

- CO8A2_MOUSE

UniProt

P25318 - CO8A2_MOUSE

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Protein

Collagen alpha-2(VIII) chain

Gene

Col8a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis By similarity.By similarity

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. camera-type eye morphogenesis Source: MGI
  3. cell adhesion Source: UniProtKB-KW
  4. epithelial cell proliferation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Angiogenesis, Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.
REACT_199046. Assembly of collagen fibrils and other multimeric structures.
REACT_199055. Collagen degradation.
REACT_216309. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen alpha-2(VIII) chain
Alternative name(s):
Endothelial collagen
Gene namesi
Name:Col8a2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:88464. Col8a2.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: UniProtKB-KW
  2. collagen trimer Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

COL8A1-/COL8A2- mice exhibit decreased proliferation of mesangial cells, reduced phosphorylation of ERK1/2 and increased p27(KIP1) expression. Diabetic COL8A1-/COL8A2- mice reveal reduced mesangial expansion and cellularity and extracellular matrix expansion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 699675Collagen alpha-2(VIII) chainPRO_0000005836Add
BLAST

Post-translational modificationi

Proteolytically cleaved by neutrophil elastase, in vitro.By similarity
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Keywords - PTMi

Hydroxylation

Proteomic databases

MaxQBiP25318.
PRIDEiP25318.

PTM databases

PhosphoSiteiP25318.

Expressioni

Tissue specificityi

In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells.1 Publication

Gene expression databases

BgeeiP25318.
CleanExiMM_COL8A2.
ExpressionAtlasiP25318. baseline and differential.
GenevestigatoriP25318.

Interactioni

Subunit structurei

Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrhedron stabilized by central interacting C-terminal NC1 trimers By similarity.By similarity

Structurei

3D structure databases

ProteinModelPortaliP25318.
SMRiP25318. Positions 571-698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini566 – 699134C1qPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 7248Nonhelical region (NC2)Add
BLAST
Regioni73 – 532460Triple-helical region (COL1)Add
BLAST
Regioni533 – 699167Nonhelical region (NC1)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 563547Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 C1q domain.PROSITE-ProRule annotation

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiNOG114228.
GeneTreeiENSGT00760000118830.
HOGENOMiHOG000085653.
HOVERGENiHBG108220.
InParanoidiP25318.
OMAiAGPPFRE.
OrthoDBiEOG70ZZPW.
PhylomeDBiP25318.
TreeFamiTF334029.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00386. C1q. 1 hit.
PF01391. Collagen. 2 hits.
[Graphical view]
PRINTSiPR00007. COMPLEMNTC1Q.
SMARTiSM00110. C1Q. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS50871. C1Q. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P25318-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQGALMPLPS LLLLLLGCGP RVSSGGGAGG AAGYAPVKYV QPMQKGPVGP
60 70 80 90 100
PFREGKGQYL EMPLPMLPMD LKGEPGPPGK PGPRGPPGPP GFPGKPGTGK
110 120 130 140 150
PGVHGQPGPA GPPGFSRMGK AGPPGLPGKV GPPGQPGLRG EPGIRGDQGL
160 170 180 190 200
RGPPGPPGLP GPSGITVPGK PGAQGAPGPP GFRGEPGPQG EPGPRGDRGL
210 220 230 240 250
KGDNGVGQPG LPGAPGQAGA PGPPGLPGPA GLGKPGLDGI PGAPGDKGDS
260 270 280 290 300
GPPGVPGSRG EPGAVGPKGP PGVDGVGIPG AAGVPGPQGP VGAKGEPGLR
310 320 330 340 350
GPPGLIGPVG YGMPGKPGPK GDRGPVGAPG LLGDRGEPGE DGKPGEQGPQ
360 370 380 390 400
GLGGPPGLPG SAGLPGRRGP PGSKGEVGPG GPPGVPGIRG DQGPNGLAGK
410 420 430 440 450
PGLPGERGLP GAHGPPGPTG PKGEPGFTGR PGGPGVAGAL GQKGDLGLPG
460 470 480 490 500
QPGLRGPSGI PGLQGPAGPI GPQGLPGLKG EPGLPGPPGE GKVGEPGSAG
510 520 530 540 550
PTGPPGVPGS PGLTGPPGPP GPPGPPGAPG ALDETGIAGL HLPNGGVEGA
560 570 580 590 600
VLGKGGKPQF GLGELSAHAT PAFTAVLTSP FPASGMPVRF DRTLYNGHSG
610 620 630 640 650
YNPATGIFTC PVGGVYYFAY HVHVKGTNVW VALYKNNVPA TYTYDEYKKG
660 670 680 690
YLDQASGGAV LQLRPNDQVW VQMPSDQANG LYSTEYIHSS FSGFLLCPT
Length:699
Mass (Da):66,943
Last modified:August 16, 2005 - v2
Checksum:iFCBD4FBB44642646
GO
Isoform 2 (identifier: P25318-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-223: Missing.

Note: No experimental confirmation available.

Show »
Length:643
Mass (Da):61,831
Checksum:iFE19B6EC5AAB2A8B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti609 – 6091T → S in M60833. (PubMed:2019595)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei168 – 22356Missing in isoform 2. 1 PublicationVSP_014975Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL606976 Genomic DNA. Translation: CAM45876.1.
BC065148 mRNA. Translation: AAH65148.1.
BC080317 mRNA. Translation: AAH80317.1.
AK131153 mRNA. Translation: BAD21403.1.
M60833 Genomic DNA. No translation available.
CCDSiCCDS18649.1. [P25318-1]
PIRiB57131.
RefSeqiNP_955767.1. NM_199473.2. [P25318-1]
UniGeneiMm.296327.

Genome annotation databases

EnsembliENSMUST00000070132; ENSMUSP00000070270; ENSMUSG00000056174. [P25318-1]
GeneIDi329941.
KEGGimmu:329941.
UCSCiuc008utd.1. mouse. [P25318-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL606976 Genomic DNA. Translation: CAM45876.1 .
BC065148 mRNA. Translation: AAH65148.1 .
BC080317 mRNA. Translation: AAH80317.1 .
AK131153 mRNA. Translation: BAD21403.1 .
M60833 Genomic DNA. No translation available.
CCDSi CCDS18649.1. [P25318-1 ]
PIRi B57131.
RefSeqi NP_955767.1. NM_199473.2. [P25318-1 ]
UniGenei Mm.296327.

3D structure databases

ProteinModelPortali P25318.
SMRi P25318. Positions 571-698.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P25318.

Proteomic databases

MaxQBi P25318.
PRIDEi P25318.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000070132 ; ENSMUSP00000070270 ; ENSMUSG00000056174 . [P25318-1 ]
GeneIDi 329941.
KEGGi mmu:329941.
UCSCi uc008utd.1. mouse. [P25318-1 ]

Organism-specific databases

CTDi 1296.
MGIi MGI:88464. Col8a2.

Phylogenomic databases

eggNOGi NOG114228.
GeneTreei ENSGT00760000118830.
HOGENOMi HOG000085653.
HOVERGENi HBG108220.
InParanoidi P25318.
OMAi AGPPFRE.
OrthoDBi EOG70ZZPW.
PhylomeDBi P25318.
TreeFami TF334029.

Enzyme and pathway databases

Reactomei REACT_198984. Collagen biosynthesis and modifying enzymes.
REACT_199046. Assembly of collagen fibrils and other multimeric structures.
REACT_199055. Collagen degradation.
REACT_216309. Integrin cell surface interactions.

Miscellaneous databases

NextBioi 399063.
PROi P25318.
SOURCEi Search...

Gene expression databases

Bgeei P25318.
CleanExi MM_COL8A2.
ExpressionAtlasi P25318. baseline and differential.
Genevestigatori P25318.

Family and domain databases

Gene3Di 2.60.120.40. 1 hit.
InterProi IPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view ]
Pfami PF00386. C1q. 1 hit.
PF01391. Collagen. 2 hits.
[Graphical view ]
PRINTSi PR00007. COMPLEMNTC1Q.
SMARTi SM00110. C1Q. 1 hit.
[Graphical view ]
SUPFAMi SSF49842. SSF49842. 1 hit.
PROSITEi PS50871. C1Q. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
    DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-699 (ISOFORM 1).
    Tissue: Embryonic tail.
  4. "The alpha 2(VIII) collagen gene. A novel member of the short chain collagen family located on the human chromosome 1."
    Muragaki Y., Jacenko O., Apte S., Mattei M.-G., Ninomiya Y., Olsen B.R.
    J. Biol. Chem. 266:7721-7727(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 530-699.
  5. "Lack of type VIII collagen in mice ameliorates diabetic nephropathy."
    Hopfer U., Hopfer H., Meyer-Schwesinger C., Loeffler I., Fukai N., Olsen B.R., Stahl R.A., Wolf G.
    Diabetes 58:1672-1681(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiCO8A2_MOUSE
AccessioniPrimary (citable) accession number: P25318
Secondary accession number(s): A3KFY2
, Q68ED0, Q6KAQ4, Q6P1C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: August 16, 2005
Last modified: October 29, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3