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P25303

- SCJ1_YEAST

UniProt

P25303 - SCJ1_YEAST

Protein

DnaJ-related protein SCJ1

Gene

SCJ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 2 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Regulates protein folding in the endoplasmic reticulum lumen. Probably acts as a J-protein for the Hsp70-type chaperone KAR2 by stimulating its ATP-dependent reaction cycle and initiating folding reactions. Also involved in the endoplasmic reticulum-associated degradation (ERAD) process. Cooperates with KAR2 and another J-protein JEM1 to facilitate the export of ERAD substrates to the cytoplasm by maintaining them in a translocation-competent state and preventing their aggregation in the endoplasmic reticulum lumen.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri156 – 23782CR-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chaperone binding Source: SGD
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    2. protein folding in endoplasmic reticulum Source: SGD
    3. protein transport Source: UniProtKB-KW
    4. response to unfolded protein Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32897-MONOMER.
    ReactomeiREACT_188969. XBP1(S) activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DnaJ-related protein SCJ1
    Short name:
    J protein SCJ1
    Gene namesi
    Name:SCJ1
    Ordered Locus Names:YMR214W
    ORF Names:YM8261.08
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR214w.
    SGDiS000004827. SCJ1.

    Subcellular locationi

    Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 377356DnaJ-related protein SCJ1PRO_0000071091Add
    BLAST

    Proteomic databases

    MaxQBiP25303.
    PaxDbiP25303.
    PeptideAtlasiP25303.

    Expressioni

    Gene expression databases

    GenevestigatoriP25303.

    Interactioni

    Protein-protein interaction databases

    BioGridi35392. 94 interactions.
    DIPiDIP-2395N.
    IntActiP25303. 10 interactions.
    MINTiMINT-639819.
    STRINGi4932.YMR214W.

    Structurei

    3D structure databases

    ProteinModelPortaliP25303.
    SMRiP25303. Positions 22-128, 137-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 8866JPROSITE-ProRule annotationAdd
    BLAST
    Repeati169 – 1768CXXCXGXG motif
    Repeati185 – 1928CXXCXGXG motif
    Repeati211 – 2188CXXCXGXG motif
    Repeati225 – 2328CXXCXGXG motif

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi288 – 2903Cell attachment siteSequence Analysis
    Motifi374 – 3774Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi95 – 13238Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CR-type zinc finger.PROSITE-ProRule annotation
    Contains 1 J domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri156 – 23782CR-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Signal, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0484.
    GeneTreeiENSGT00720000108827.
    HOGENOMiHOG000226718.
    KOiK14002.
    OMAiGALHVEY.
    OrthoDBiEOG77M90H.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    InterProiIPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view]
    PfamiPF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25303-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIPKLYIHLI LSLLLLPLIL AQDYYAILEI DKDATEKEIK SAYRQLSKKY    50
    HPDKNAGSEE AHQKFIEVGE AYDVLSDPEK KKIYDQFGAD AVKNGGGGGG 100
    PGGPGAGGFH DPFDIFERMF QGGHGGPGGG FGQRQRQRGP MIKVQEKLSL 150
    KQFYSGSSIE FTLNLNDECD ACHGSGSADG KLAQCPDCQG RGVIIQVLRM 200
    GIMTQQIQQM CGRCGGTGQI IKNECKTCHG KKVTKKNKFF HVDVPPGAPR 250
    NYMDTRVGEA EKGPDFDAGD LVIEFKEKDT ENMGYRRRGD NLYRTEVLSA 300
    AEALYGGWQR TIEFLDENKP VKLSRPAHVV VSNGEVEVVK GFGMPKGSKG 350
    YGDLYIDYVV VMPKTFKSGQ NMLKDEL 377
    Length:377
    Mass (Da):41,543
    Last modified:September 5, 2006 - v2
    Checksum:iFD0226902F33E2B8
    GO

    Sequence cautioni

    The sequence CAA41529.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA89929.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58679 Genomic DNA. Translation: CAA41529.1. Different initiation.
    Z49809 Genomic DNA. Translation: CAA89929.1. Different initiation.
    BK006946 Genomic DNA. Translation: DAA10113.1.
    PIRiS13648.
    RefSeqiNP_013941.2. NM_001182721.1.

    Genome annotation databases

    EnsemblFungiiYMR214W; YMR214W; YMR214W.
    GeneIDi855254.
    KEGGisce:YMR214W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58679 Genomic DNA. Translation: CAA41529.1 . Different initiation.
    Z49809 Genomic DNA. Translation: CAA89929.1 . Different initiation.
    BK006946 Genomic DNA. Translation: DAA10113.1 .
    PIRi S13648.
    RefSeqi NP_013941.2. NM_001182721.1.

    3D structure databases

    ProteinModelPortali P25303.
    SMRi P25303. Positions 22-128, 137-364.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35392. 94 interactions.
    DIPi DIP-2395N.
    IntActi P25303. 10 interactions.
    MINTi MINT-639819.
    STRINGi 4932.YMR214W.

    Proteomic databases

    MaxQBi P25303.
    PaxDbi P25303.
    PeptideAtlasi P25303.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR214W ; YMR214W ; YMR214W .
    GeneIDi 855254.
    KEGGi sce:YMR214W.

    Organism-specific databases

    CYGDi YMR214w.
    SGDi S000004827. SCJ1.

    Phylogenomic databases

    eggNOGi COG0484.
    GeneTreei ENSGT00720000108827.
    HOGENOMi HOG000226718.
    KOi K14002.
    OMAi GALHVEY.
    OrthoDBi EOG77M90H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32897-MONOMER.
    Reactomei REACT_188969. XBP1(S) activates chaperone genes.

    Miscellaneous databases

    NextBioi 978834.

    Gene expression databases

    Genevestigatori P25303.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    InterProi IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view ]
    Pfami PF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A homologue of the bacterial heat-shock gene DnaJ that alters protein sorting in yeast."
      Blumberg H., Silver P.A.
      Nature 349:627-630(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A yeast DnaJ homologue, Scj1p, can function in the endoplasmic reticulum with BiP/Kar2p via a conserved domain that specifies interactions with Hsp70s."
      Schlenstedt G., Harris S., Risse B., Lill R., Silver P.A.
      J. Cell Biol. 129:979-988(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION OF PROBABLE INITIATION SITE.
    5. "A role for the DnaJ homologue Scj1p in protein folding in the yeast endoplasmic reticulum."
      Silberstein S., Schlenstedt G., Silver P.A., Gilmore R.
      J. Cell Biol. 143:921-933(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Molecular chaperones in the yeast endoplasmic reticulum maintain the solubility of proteins for retrotranslocation and degradation."
      Nishikawa S.I., Fewell S.W., Kato Y., Brodsky J.L., Endo T.
      J. Cell Biol. 153:1061-1070(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSCJ1_YEAST
    AccessioniPrimary (citable) accession number: P25303
    Secondary accession number(s): D6W039
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 143 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8260 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3