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Protein

DnaJ-related protein SCJ1

Gene

SCJ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulates protein folding in the endoplasmic reticulum lumen. Probably acts as a J-protein for the Hsp70-type chaperone KAR2 by stimulating its ATP-dependent reaction cycle and initiating folding reactions. Also involved in the endoplasmic reticulum-associated degradation (ERAD) process. Cooperates with KAR2 and another J-protein JEM1 to facilitate the export of ERAD substrates to the cytoplasm by maintaining them in a translocation-competent state and preventing their aggregation in the endoplasmic reticulum lumen.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri156 – 23782CR-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chaperone binding Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • protein folding in endoplasmic reticulum Source: SGD
  • protein transport Source: UniProtKB-KW
  • response to unfolded protein Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32897-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ-related protein SCJ1
Short name:
J protein SCJ1
Gene namesi
Name:SCJ1
Ordered Locus Names:YMR214W
ORF Names:YM8261.08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR214w.
EuPathDBiFungiDB:YMR214W.
SGDiS000004827. SCJ1.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • endoplasmic reticulum lumen Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 377356DnaJ-related protein SCJ1PRO_0000071091Add
BLAST

Proteomic databases

MaxQBiP25303.
PaxDbiP25303.
PeptideAtlasiP25303.

Interactioni

Protein-protein interaction databases

BioGridi35392. 95 interactions.
DIPiDIP-2395N.
IntActiP25303. 10 interactions.
MINTiMINT-639819.
STRINGi4932.YMR214W.

Structurei

3D structure databases

ProteinModelPortaliP25303.
SMRiP25303. Positions 22-128, 137-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 8866JPROSITE-ProRule annotationAdd
BLAST
Repeati169 – 1768CXXCXGXG motif
Repeati185 – 1928CXXCXGXG motif
Repeati211 – 2188CXXCXGXG motif
Repeati225 – 2328CXXCXGXG motif

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi288 – 2903Cell attachment siteSequence Analysis
Motifi374 – 3774Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi95 – 13238Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 CR-type zinc finger.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri156 – 23782CR-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Signal, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
GeneTreeiENSGT00780000122011.
HOGENOMiHOG000226718.
InParanoidiP25303.
KOiK14002.
OMAiHICEACE.
OrthoDBiEOG77M90H.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPKLYIHLI LSLLLLPLIL AQDYYAILEI DKDATEKEIK SAYRQLSKKY
60 70 80 90 100
HPDKNAGSEE AHQKFIEVGE AYDVLSDPEK KKIYDQFGAD AVKNGGGGGG
110 120 130 140 150
PGGPGAGGFH DPFDIFERMF QGGHGGPGGG FGQRQRQRGP MIKVQEKLSL
160 170 180 190 200
KQFYSGSSIE FTLNLNDECD ACHGSGSADG KLAQCPDCQG RGVIIQVLRM
210 220 230 240 250
GIMTQQIQQM CGRCGGTGQI IKNECKTCHG KKVTKKNKFF HVDVPPGAPR
260 270 280 290 300
NYMDTRVGEA EKGPDFDAGD LVIEFKEKDT ENMGYRRRGD NLYRTEVLSA
310 320 330 340 350
AEALYGGWQR TIEFLDENKP VKLSRPAHVV VSNGEVEVVK GFGMPKGSKG
360 370
YGDLYIDYVV VMPKTFKSGQ NMLKDEL
Length:377
Mass (Da):41,543
Last modified:September 5, 2006 - v2
Checksum:iFD0226902F33E2B8
GO

Sequence cautioni

The sequence CAA41529.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA89929.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58679 Genomic DNA. Translation: CAA41529.1. Different initiation.
Z49809 Genomic DNA. Translation: CAA89929.1. Different initiation.
BK006946 Genomic DNA. Translation: DAA10113.1.
PIRiS13648.
RefSeqiNP_013941.2. NM_001182721.1.

Genome annotation databases

EnsemblFungiiYMR214W; YMR214W; YMR214W.
GeneIDi855254.
KEGGisce:YMR214W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58679 Genomic DNA. Translation: CAA41529.1. Different initiation.
Z49809 Genomic DNA. Translation: CAA89929.1. Different initiation.
BK006946 Genomic DNA. Translation: DAA10113.1.
PIRiS13648.
RefSeqiNP_013941.2. NM_001182721.1.

3D structure databases

ProteinModelPortaliP25303.
SMRiP25303. Positions 22-128, 137-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35392. 95 interactions.
DIPiDIP-2395N.
IntActiP25303. 10 interactions.
MINTiMINT-639819.
STRINGi4932.YMR214W.

Proteomic databases

MaxQBiP25303.
PaxDbiP25303.
PeptideAtlasiP25303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR214W; YMR214W; YMR214W.
GeneIDi855254.
KEGGisce:YMR214W.

Organism-specific databases

CYGDiYMR214w.
EuPathDBiFungiDB:YMR214W.
SGDiS000004827. SCJ1.

Phylogenomic databases

eggNOGiCOG0484.
GeneTreeiENSGT00780000122011.
HOGENOMiHOG000226718.
InParanoidiP25303.
KOiK14002.
OMAiHICEACE.
OrthoDBiEOG77M90H.

Enzyme and pathway databases

BioCyciYEAST:G3O-32897-MONOMER.

Miscellaneous databases

NextBioi978834.
PROiP25303.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A homologue of the bacterial heat-shock gene DnaJ that alters protein sorting in yeast."
    Blumberg H., Silver P.A.
    Nature 349:627-630(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A yeast DnaJ homologue, Scj1p, can function in the endoplasmic reticulum with BiP/Kar2p via a conserved domain that specifies interactions with Hsp70s."
    Schlenstedt G., Harris S., Risse B., Lill R., Silver P.A.
    J. Cell Biol. 129:979-988(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION OF PROBABLE INITIATION SITE.
  5. "A role for the DnaJ homologue Scj1p in protein folding in the yeast endoplasmic reticulum."
    Silberstein S., Schlenstedt G., Silver P.A., Gilmore R.
    J. Cell Biol. 143:921-933(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Molecular chaperones in the yeast endoplasmic reticulum maintain the solubility of proteins for retrotranslocation and degradation."
    Nishikawa S.I., Fewell S.W., Kato Y., Brodsky J.L., Endo T.
    J. Cell Biol. 153:1061-1070(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSCJ1_YEAST
AccessioniPrimary (citable) accession number: P25303
Secondary accession number(s): D6W039
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 5, 2006
Last modified: June 24, 2015
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.