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P25303 (SCJ1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DnaJ-related protein SCJ1

Short name=J protein SCJ1
Gene names
Name:SCJ1
Ordered Locus Names:YMR214W
ORF Names:YM8261.08
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates protein folding in the endoplasmic reticulum lumen. Probably acts as a J-protein for the Hsp70-type chaperone KAR2 by stimulating its ATP-dependent reaction cycle and initiating folding reactions. Also involved in the endoplasmic reticulum-associated degradation (ERAD) process. Cooperates with KAR2 and another J-protein JEM1 to facilitate the export of ERAD substrates to the cytoplasm by maintaining them in a translocation-competent state and preventing their aggregation in the endoplasmic reticulum lumen. Ref.5 Ref.6

Subcellular location

Endoplasmic reticulum lumen Ref.4.

Miscellaneous

Present with 8260 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 CR-type zinc finger.

Contains 1 J domain.

Sequence caution

The sequence CAA41529.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA89929.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 377356DnaJ-related protein SCJ1
PRO_0000071091

Regions

Domain23 – 8866J
Repeat169 – 1768CXXCXGXG motif
Repeat185 – 1928CXXCXGXG motif
Repeat211 – 2188CXXCXGXG motif
Repeat225 – 2328CXXCXGXG motif
Zinc finger156 – 23782CR-type
Motif288 – 2903Cell attachment site Potential
Motif374 – 3774Prevents secretion from ER Potential
Compositional bias95 – 13238Gly-rich

Sequences

Sequence LengthMass (Da)Tools
P25303 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: FD0226902F33E2B8

FASTA37741,543
        10         20         30         40         50         60 
MIPKLYIHLI LSLLLLPLIL AQDYYAILEI DKDATEKEIK SAYRQLSKKY HPDKNAGSEE 

        70         80         90        100        110        120 
AHQKFIEVGE AYDVLSDPEK KKIYDQFGAD AVKNGGGGGG PGGPGAGGFH DPFDIFERMF 

       130        140        150        160        170        180 
QGGHGGPGGG FGQRQRQRGP MIKVQEKLSL KQFYSGSSIE FTLNLNDECD ACHGSGSADG 

       190        200        210        220        230        240 
KLAQCPDCQG RGVIIQVLRM GIMTQQIQQM CGRCGGTGQI IKNECKTCHG KKVTKKNKFF 

       250        260        270        280        290        300 
HVDVPPGAPR NYMDTRVGEA EKGPDFDAGD LVIEFKEKDT ENMGYRRRGD NLYRTEVLSA 

       310        320        330        340        350        360 
AEALYGGWQR TIEFLDENKP VKLSRPAHVV VSNGEVEVVK GFGMPKGSKG YGDLYIDYVV 

       370 
VMPKTFKSGQ NMLKDEL 

« Hide

References

« Hide 'large scale' references
[1]"A homologue of the bacterial heat-shock gene DnaJ that alters protein sorting in yeast."
Blumberg H., Silver P.A.
Nature 349:627-630(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A yeast DnaJ homologue, Scj1p, can function in the endoplasmic reticulum with BiP/Kar2p via a conserved domain that specifies interactions with Hsp70s."
Schlenstedt G., Harris S., Risse B., Lill R., Silver P.A.
J. Cell Biol. 129:979-988(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]"A role for the DnaJ homologue Scj1p in protein folding in the yeast endoplasmic reticulum."
Silberstein S., Schlenstedt G., Silver P.A., Gilmore R.
J. Cell Biol. 143:921-933(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Molecular chaperones in the yeast endoplasmic reticulum maintain the solubility of proteins for retrotranslocation and degradation."
Nishikawa S.I., Fewell S.W., Kato Y., Brodsky J.L., Endo T.
J. Cell Biol. 153:1061-1070(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58679 Genomic DNA. Translation: CAA41529.1. Different initiation.
Z49809 Genomic DNA. Translation: CAA89929.1. Different initiation.
BK006946 Genomic DNA. Translation: DAA10113.1.
PIRS13648.
RefSeqNP_013941.2. NM_001182721.1.

3D structure databases

ProteinModelPortalP25303.
SMRP25303. Positions 22-128, 137-364.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35392. 94 interactions.
DIPDIP-2395N.
IntActP25303. 10 interactions.
MINTMINT-639819.
STRING4932.YMR214W.

Proteomic databases

MaxQBP25303.
PaxDbP25303.
PeptideAtlasP25303.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR214W; YMR214W; YMR214W.
GeneID855254.
KEGGsce:YMR214W.

Organism-specific databases

CYGDYMR214w.
SGDS000004827. SCJ1.

Phylogenomic databases

eggNOGCOG0484.
GeneTreeENSGT00720000108827.
HOGENOMHOG000226718.
KOK14002.
OMAGALHVEY.
OrthoDBEOG77M90H.

Enzyme and pathway databases

BioCycYEAST:G3O-32897-MONOMER.

Gene expression databases

GenevestigatorP25303.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
InterProIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978834.

Entry information

Entry nameSCJ1_YEAST
AccessionPrimary (citable) accession number: P25303
Secondary accession number(s): D6W039
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 5, 2006
Last modified: July 9, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families