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P25302 (SWI4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulatory protein SWI4
Alternative name(s):
Cell-cycle box factor subunit SWI4
Protein ART1
Gene names
Name:SWI4
Synonyms:ART1
Ordered Locus Names:YER111C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1093 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of a complex involved in cell-cycle-dependent transcription. SWI4 and SWI6 are required for formation of the cell-cycle box factor-DNA complex. The repeated element in the upstream region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB).

Subunit structure

Component of the transcription complex SCB-binding factor (SBF) composed of SWI6 and SWI4. Interacts with MSA2. Ref.7

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.

Sequence similarities

Contains 2 ANK repeats.

Contains 1 HTH APSES-type DNA-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself4EBI-18626,EBI-18626
SWI6P099597EBI-18626,EBI-18641

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10931093Regulatory protein SWI4
PRO_0000067069

Regions

Domain37 – 147111HTH APSES-type
Repeat520 – 54930ANK 1
Repeat641 – 67030ANK 2
DNA binding71 – 9222H-T-H motif By similarity
Compositional bias201 – 727527Asn/Gln-rich

Amino acid modifications

Modified residue2551Phosphoserine Ref.8
Modified residue8061Phosphoserine Ref.8

Experimental info

Sequence conflict1751R → T in CAA35949. Ref.1
Sequence conflict4311V → I in CAA35949. Ref.1
Sequence conflict10541A → L in CAA35949. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25302 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 40EA2EA7732687A9

FASTA1,093123,806
        10         20         30         40         50         60 
MPFDVLISNQ KDNTNHQNIT PISKSVLLAP HSNHPVIEIA TYSETDVYEC YIRGFETKIV 

        70         80         90        100        110        120 
MRRTKDDWIN ITQVFKIAQF SKTKRTKILE KESNDMQHEK VQGGYGRFQG TWIPLDSAKF 

       130        140        150        160        170        180 
LVNKYEIIDP VVNSILTFQF DPNNPPPKRS KNSILRKTSP GTKITSPSSY NKTPRKKNSS 

       190        200        210        220        230        240 
SSTSATTTAA NKKGKKNASI NQPNPSPLQN LVFQTPQQFQ VNSSMNIMNN NDNHTTMNFN 

       250        260        270        280        290        300 
NDTRHNLINN ISNNSNQSTI IQQQKSIHEN SFNNNYSATQ KPLQFFPIPT NLQNKNVALN 

       310        320        330        340        350        360 
NPNNNDSNSY SHNIDNVINS SNNNNNGNNN NLIIVPDGPM QSQQQQQHHH EYLTNNFNHS 

       370        380        390        400        410        420 
MMDSITNGNS KKRRKKLNQS NEQQFYNQQE KIQRHFKLMK QPLLWQSFQN PNDHHNEYCD 

       430        440        450        460        470        480 
SNGSNNNNNT VASNGSSIEV FSSNENDNSM NMSSRSMTPF SAGNTSSQNK LENKMTDQEY 

       490        500        510        520        530        540 
KQTILTILSS ERSSDVDQAL LATLYPAPKN FNINFEIDDQ GHTPLHWATA MANIPLIKML 

       550        560        570        580        590        600 
ITLNANALQC NKLGFNCITK SIFYNNCYKE NAFDEIISIL KICLITPDVN GRLPFHYLIE 

       610        620        630        640        650        660 
LSVNKSKNPM IIKSYMDSII LSLGQQDYNL LKICLNYQDN IGNTPLHLSA LNLNFEVYNR 

       670        680        690        700        710        720 
LVYLGASTDI LNLDNESPAS IMNKFNTPAG GSNSRNNNTK ADRKLARNLP QKNYYQQQQQ 

       730        740        750        760        770        780 
QQQPQNNVKI PKIIKTQHPD KEDSTADVNI AKTDSEVNES QYLHSNQPNS TNMNTIMEDL 

       790        800        810        820        830        840 
SNINSFVTSS VIKDIKSTPS KILENSPILY RRRSQSISDE KEKAKDNENQ VEKKKDPLNS 

       850        860        870        880        890        900 
VKTAMPSLES PSSLLPIQMS PLGKYSKPLS QQINKLNTKV SSLQRIMGEE IKNLDNEVVE 

       910        920        930        940        950        960 
TESSISNNKK RLITIAHQIE DAFDSVSNKT PINSISDLQS RIKETSSKLN SEKQNFIQSL 

       970        980        990       1000       1010       1020 
EKSQALKLAT IVQDEESKVD MNTNSSSHPE KQEDEEPIPK STSETSSPKN TKADAKFSNT 

      1030       1040       1050       1060       1070       1080 
VQESYDVNET LRLATELTIL QFKRRMTTLK ISEAKSKINS SVKLDKYRNL IGITIENIDS 

      1090 
KLDDIEKDLR ANA 

« Hide

References

« Hide 'large scale' references
[1]"The yeast SW14 protein contains a present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription."
Andrews B.J., Herskowitz I.
Nature 342:830-833(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast."
Daniel J.
Mol. Gen. Genet. 240:245-257(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 845-1093.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MSA2.
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-255 AND SER-806, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X51606 Genomic DNA. Translation: CAA35949.1.
U18916 Genomic DNA. Translation: AAC03209.1.
BK006939 Genomic DNA. Translation: DAA07771.1.
PIRS50614.
RefSeqNP_011036.1. NM_001179001.1.

3D structure databases

ProteinModelPortalP25302.
SMRP25302. Positions 40-129, 469-678.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36856. 416 interactions.
DIPDIP-847N.
IntActP25302. 75 interactions.
MINTMINT-698971.
STRING4932.YER111C.

Proteomic databases

MaxQBP25302.
PaxDbP25302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER111C; YER111C; YER111C.
GeneID856847.
KEGGsce:YER111C.

Organism-specific databases

CYGDYER111c.
SGDS000000913. SWI4.

Phylogenomic databases

eggNOGCOG0666.
GeneTreeENSGT00530000067565.
HOGENOMHOG000141812.
KOK06649.
OMAINTDING.
OrthoDBEOG7WMCTB.

Enzyme and pathway databases

BioCycYEAST:G3O-30275-MONOMER.

Gene expression databases

GenevestigatorP25302.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
3.10.260.10. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018004. KilA_N/APSES_HTH.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view]
PfamPF00023. Ank. 2 hits.
PF04383. KilA-N. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF54616. SSF54616. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 2 hits.
PS51299. HTH_APSES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983174.

Entry information

Entry nameSWI4_YEAST
AccessionPrimary (citable) accession number: P25302
Secondary accession number(s): D3DM17
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families