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P25302

- SWI4_YEAST

UniProt

P25302 - SWI4_YEAST

Protein

Regulatory protein SWI4

Gene

SWI4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Part of a complex involved in cell-cycle-dependent transcription. SWI4 and SWI6 are required for formation of the cell-cycle box factor-DNA complex. The repeated element in the upstream region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi71 – 9222H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. protein binding Source: IntAct
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD

    GO - Biological processi

    1. positive regulation of transcription from RNA polymerase II promoter in response to heat stress Source: SGD
    2. positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30275-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein SWI4
    Alternative name(s):
    Cell-cycle box factor subunit SWI4
    Protein ART1
    Gene namesi
    Name:SWI4
    Synonyms:ART1
    Ordered Locus Names:YER111C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER111c.
    SGDiS000000913. SWI4.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear chromatin Source: SGD
    2. SBF transcription complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10931093Regulatory protein SWI4PRO_0000067069Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei255 – 2551Phosphoserine1 Publication
    Modified residuei806 – 8061Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP25302.
    PaxDbiP25302.

    Expressioni

    Gene expression databases

    GenevestigatoriP25302.

    Interactioni

    Subunit structurei

    Component of the transcription complex SCB-binding factor (SBF) composed of SWI6 and SWI4. Interacts with MSA2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-18626,EBI-18626
    SWI6P099597EBI-18626,EBI-18641

    Protein-protein interaction databases

    BioGridi36856. 419 interactions.
    DIPiDIP-847N.
    IntActiP25302. 75 interactions.
    MINTiMINT-698971.
    STRINGi4932.YER111C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25302.
    SMRiP25302. Positions 40-129, 469-678.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 147111HTH APSES-typePROSITE-ProRule annotationAdd
    BLAST
    Repeati520 – 54930ANK 1Add
    BLAST
    Repeati641 – 67030ANK 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi201 – 727527Asn/Gln-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ANK repeats.PROSITE-ProRule annotation
    Contains 1 HTH APSES-type DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00530000067565.
    HOGENOMiHOG000141812.
    KOiK06649.
    OMAiINTDING.
    OrthoDBiEOG7WMCTB.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.10.260.10. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR018004. KilA_N/APSES_HTH.
    IPR003163. Tscrpt_reg_HTH_APSES-type.
    [Graphical view]
    PfamiPF00023. Ank. 2 hits.
    PF04383. KilA-N. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF54616. SSF54616. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 2 hits.
    PS50088. ANK_REPEAT. 2 hits.
    PS51299. HTH_APSES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25302-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPFDVLISNQ KDNTNHQNIT PISKSVLLAP HSNHPVIEIA TYSETDVYEC     50
    YIRGFETKIV MRRTKDDWIN ITQVFKIAQF SKTKRTKILE KESNDMQHEK 100
    VQGGYGRFQG TWIPLDSAKF LVNKYEIIDP VVNSILTFQF DPNNPPPKRS 150
    KNSILRKTSP GTKITSPSSY NKTPRKKNSS SSTSATTTAA NKKGKKNASI 200
    NQPNPSPLQN LVFQTPQQFQ VNSSMNIMNN NDNHTTMNFN NDTRHNLINN 250
    ISNNSNQSTI IQQQKSIHEN SFNNNYSATQ KPLQFFPIPT NLQNKNVALN 300
    NPNNNDSNSY SHNIDNVINS SNNNNNGNNN NLIIVPDGPM QSQQQQQHHH 350
    EYLTNNFNHS MMDSITNGNS KKRRKKLNQS NEQQFYNQQE KIQRHFKLMK 400
    QPLLWQSFQN PNDHHNEYCD SNGSNNNNNT VASNGSSIEV FSSNENDNSM 450
    NMSSRSMTPF SAGNTSSQNK LENKMTDQEY KQTILTILSS ERSSDVDQAL 500
    LATLYPAPKN FNINFEIDDQ GHTPLHWATA MANIPLIKML ITLNANALQC 550
    NKLGFNCITK SIFYNNCYKE NAFDEIISIL KICLITPDVN GRLPFHYLIE 600
    LSVNKSKNPM IIKSYMDSII LSLGQQDYNL LKICLNYQDN IGNTPLHLSA 650
    LNLNFEVYNR LVYLGASTDI LNLDNESPAS IMNKFNTPAG GSNSRNNNTK 700
    ADRKLARNLP QKNYYQQQQQ QQQPQNNVKI PKIIKTQHPD KEDSTADVNI 750
    AKTDSEVNES QYLHSNQPNS TNMNTIMEDL SNINSFVTSS VIKDIKSTPS 800
    KILENSPILY RRRSQSISDE KEKAKDNENQ VEKKKDPLNS VKTAMPSLES 850
    PSSLLPIQMS PLGKYSKPLS QQINKLNTKV SSLQRIMGEE IKNLDNEVVE 900
    TESSISNNKK RLITIAHQIE DAFDSVSNKT PINSISDLQS RIKETSSKLN 950
    SEKQNFIQSL EKSQALKLAT IVQDEESKVD MNTNSSSHPE KQEDEEPIPK 1000
    STSETSSPKN TKADAKFSNT VQESYDVNET LRLATELTIL QFKRRMTTLK 1050
    ISEAKSKINS SVKLDKYRNL IGITIENIDS KLDDIEKDLR ANA 1093
    Length:1,093
    Mass (Da):123,806
    Last modified:February 1, 1995 - v2
    Checksum:i40EA2EA7732687A9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti175 – 1751R → T in CAA35949. (PubMed:2689885)Curated
    Sequence conflicti431 – 4311V → I in CAA35949. (PubMed:2689885)Curated
    Sequence conflicti1054 – 10541A → L in CAA35949. (PubMed:2689885)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51606 Genomic DNA. Translation: CAA35949.1.
    U18916 Genomic DNA. Translation: AAC03209.1.
    BK006939 Genomic DNA. Translation: DAA07771.1.
    PIRiS50614.
    RefSeqiNP_011036.1. NM_001179001.1.

    Genome annotation databases

    EnsemblFungiiYER111C; YER111C; YER111C.
    GeneIDi856847.
    KEGGisce:YER111C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51606 Genomic DNA. Translation: CAA35949.1 .
    U18916 Genomic DNA. Translation: AAC03209.1 .
    BK006939 Genomic DNA. Translation: DAA07771.1 .
    PIRi S50614.
    RefSeqi NP_011036.1. NM_001179001.1.

    3D structure databases

    ProteinModelPortali P25302.
    SMRi P25302. Positions 40-129, 469-678.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36856. 419 interactions.
    DIPi DIP-847N.
    IntActi P25302. 75 interactions.
    MINTi MINT-698971.
    STRINGi 4932.YER111C.

    Proteomic databases

    MaxQBi P25302.
    PaxDbi P25302.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER111C ; YER111C ; YER111C .
    GeneIDi 856847.
    KEGGi sce:YER111C.

    Organism-specific databases

    CYGDi YER111c.
    SGDi S000000913. SWI4.

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00530000067565.
    HOGENOMi HOG000141812.
    KOi K06649.
    OMAi INTDING.
    OrthoDBi EOG7WMCTB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30275-MONOMER.

    Miscellaneous databases

    NextBioi 983174.

    Gene expression databases

    Genevestigatori P25302.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    3.10.260.10. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR018004. KilA_N/APSES_HTH.
    IPR003163. Tscrpt_reg_HTH_APSES-type.
    [Graphical view ]
    Pfami PF00023. Ank. 2 hits.
    PF04383. KilA-N. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF54616. SSF54616. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 2 hits.
    PS50088. ANK_REPEAT. 2 hits.
    PS51299. HTH_APSES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast SW14 protein contains a present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription."
      Andrews B.J., Herskowitz I.
      Nature 342:830-833(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast."
      Daniel J.
      Mol. Gen. Genet. 240:245-257(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 845-1093.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
      Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
      J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MSA2.
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-255 AND SER-806, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSWI4_YEAST
    AccessioniPrimary (citable) accession number: P25302
    Secondary accession number(s): D3DM17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 589 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3