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P25302

- SWI4_YEAST

UniProt

P25302 - SWI4_YEAST

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Protein
Regulatory protein SWI4
Gene
SWI4, ART1, YER111C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Part of a complex involved in cell-cycle-dependent transcription. SWI4 and SWI6 are required for formation of the cell-cycle box factor-DNA complex. The repeated element in the upstream region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi71 – 9222H-T-H motif By similarity
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
  3. identical protein binding Source: IntAct
  4. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase II promoter in response to heat stress Source: SGD
  2. positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30275-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein SWI4
Alternative name(s):
Cell-cycle box factor subunit SWI4
Protein ART1
Gene namesi
Name:SWI4
Synonyms:ART1
Ordered Locus Names:YER111C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER111c.
SGDiS000000913. SWI4.

Subcellular locationi

GO - Cellular componenti

  1. SBF transcription complex Source: SGD
  2. nuclear chromatin Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10931093Regulatory protein SWI4
PRO_0000067069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei255 – 2551Phosphoserine1 Publication
Modified residuei806 – 8061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25302.
PaxDbiP25302.

Expressioni

Gene expression databases

GenevestigatoriP25302.

Interactioni

Subunit structurei

Component of the transcription complex SCB-binding factor (SBF) composed of SWI6 and SWI4. Interacts with MSA2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-18626,EBI-18626
SWI6P099597EBI-18626,EBI-18641

Protein-protein interaction databases

BioGridi36856. 419 interactions.
DIPiDIP-847N.
IntActiP25302. 75 interactions.
MINTiMINT-698971.
STRINGi4932.YER111C.

Structurei

3D structure databases

ProteinModelPortaliP25302.
SMRiP25302. Positions 40-129, 469-678.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 147111HTH APSES-type
Add
BLAST
Repeati520 – 54930ANK 1
Add
BLAST
Repeati641 – 67030ANK 2
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi201 – 727527Asn/Gln-rich
Add
BLAST

Sequence similaritiesi

Contains 2 ANK repeats.

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00530000067565.
HOGENOMiHOG000141812.
KOiK06649.
OMAiINTDING.
OrthoDBiEOG7WMCTB.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.10.260.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018004. KilA_N/APSES_HTH.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF04383. KilA-N. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF54616. SSF54616. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 2 hits.
PS51299. HTH_APSES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25302-1 [UniParc]FASTAAdd to Basket

« Hide

MPFDVLISNQ KDNTNHQNIT PISKSVLLAP HSNHPVIEIA TYSETDVYEC     50
YIRGFETKIV MRRTKDDWIN ITQVFKIAQF SKTKRTKILE KESNDMQHEK 100
VQGGYGRFQG TWIPLDSAKF LVNKYEIIDP VVNSILTFQF DPNNPPPKRS 150
KNSILRKTSP GTKITSPSSY NKTPRKKNSS SSTSATTTAA NKKGKKNASI 200
NQPNPSPLQN LVFQTPQQFQ VNSSMNIMNN NDNHTTMNFN NDTRHNLINN 250
ISNNSNQSTI IQQQKSIHEN SFNNNYSATQ KPLQFFPIPT NLQNKNVALN 300
NPNNNDSNSY SHNIDNVINS SNNNNNGNNN NLIIVPDGPM QSQQQQQHHH 350
EYLTNNFNHS MMDSITNGNS KKRRKKLNQS NEQQFYNQQE KIQRHFKLMK 400
QPLLWQSFQN PNDHHNEYCD SNGSNNNNNT VASNGSSIEV FSSNENDNSM 450
NMSSRSMTPF SAGNTSSQNK LENKMTDQEY KQTILTILSS ERSSDVDQAL 500
LATLYPAPKN FNINFEIDDQ GHTPLHWATA MANIPLIKML ITLNANALQC 550
NKLGFNCITK SIFYNNCYKE NAFDEIISIL KICLITPDVN GRLPFHYLIE 600
LSVNKSKNPM IIKSYMDSII LSLGQQDYNL LKICLNYQDN IGNTPLHLSA 650
LNLNFEVYNR LVYLGASTDI LNLDNESPAS IMNKFNTPAG GSNSRNNNTK 700
ADRKLARNLP QKNYYQQQQQ QQQPQNNVKI PKIIKTQHPD KEDSTADVNI 750
AKTDSEVNES QYLHSNQPNS TNMNTIMEDL SNINSFVTSS VIKDIKSTPS 800
KILENSPILY RRRSQSISDE KEKAKDNENQ VEKKKDPLNS VKTAMPSLES 850
PSSLLPIQMS PLGKYSKPLS QQINKLNTKV SSLQRIMGEE IKNLDNEVVE 900
TESSISNNKK RLITIAHQIE DAFDSVSNKT PINSISDLQS RIKETSSKLN 950
SEKQNFIQSL EKSQALKLAT IVQDEESKVD MNTNSSSHPE KQEDEEPIPK 1000
STSETSSPKN TKADAKFSNT VQESYDVNET LRLATELTIL QFKRRMTTLK 1050
ISEAKSKINS SVKLDKYRNL IGITIENIDS KLDDIEKDLR ANA 1093
Length:1,093
Mass (Da):123,806
Last modified:February 1, 1995 - v2
Checksum:i40EA2EA7732687A9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1751R → T in CAA35949. 1 Publication
Sequence conflicti431 – 4311V → I in CAA35949. 1 Publication
Sequence conflicti1054 – 10541A → L in CAA35949. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51606 Genomic DNA. Translation: CAA35949.1.
U18916 Genomic DNA. Translation: AAC03209.1.
BK006939 Genomic DNA. Translation: DAA07771.1.
PIRiS50614.
RefSeqiNP_011036.1. NM_001179001.1.

Genome annotation databases

EnsemblFungiiYER111C; YER111C; YER111C.
GeneIDi856847.
KEGGisce:YER111C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51606 Genomic DNA. Translation: CAA35949.1 .
U18916 Genomic DNA. Translation: AAC03209.1 .
BK006939 Genomic DNA. Translation: DAA07771.1 .
PIRi S50614.
RefSeqi NP_011036.1. NM_001179001.1.

3D structure databases

ProteinModelPortali P25302.
SMRi P25302. Positions 40-129, 469-678.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36856. 419 interactions.
DIPi DIP-847N.
IntActi P25302. 75 interactions.
MINTi MINT-698971.
STRINGi 4932.YER111C.

Proteomic databases

MaxQBi P25302.
PaxDbi P25302.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER111C ; YER111C ; YER111C .
GeneIDi 856847.
KEGGi sce:YER111C.

Organism-specific databases

CYGDi YER111c.
SGDi S000000913. SWI4.

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00530000067565.
HOGENOMi HOG000141812.
KOi K06649.
OMAi INTDING.
OrthoDBi EOG7WMCTB.

Enzyme and pathway databases

BioCyci YEAST:G3O-30275-MONOMER.

Miscellaneous databases

NextBioi 983174.

Gene expression databases

Genevestigatori P25302.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
3.10.260.10. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018004. KilA_N/APSES_HTH.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view ]
Pfami PF00023. Ank. 2 hits.
PF04383. KilA-N. 1 hit.
[Graphical view ]
SMARTi SM00248. ANK. 2 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF54616. SSF54616. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 2 hits.
PS51299. HTH_APSES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast SW14 protein contains a present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription."
    Andrews B.J., Herskowitz I.
    Nature 342:830-833(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast."
    Daniel J.
    Mol. Gen. Genet. 240:245-257(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 845-1093.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
    Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
    J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MSA2.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-255 AND SER-806, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSWI4_YEAST
AccessioniPrimary (citable) accession number: P25302
Secondary accession number(s): D3DM17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi