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P25302

- SWI4_YEAST

UniProt

P25302 - SWI4_YEAST

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Protein

Regulatory protein SWI4

Gene

SWI4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of a complex involved in cell-cycle-dependent transcription. SWI4 and SWI6 are required for formation of the cell-cycle box factor-DNA complex. The repeated element in the upstream region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi71 – 9222H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. identical protein binding Source: IntAct
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase II promoter in response to heat stress Source: SGD
  2. positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30275-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein SWI4
Alternative name(s):
Cell-cycle box factor subunit SWI4
Protein ART1
Gene namesi
Name:SWI4
Synonyms:ART1
Ordered Locus Names:YER111C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER111c.
SGDiS000000913. SWI4.

Subcellular locationi

GO - Cellular componenti

  1. nuclear chromatin Source: SGD
  2. SBF transcription complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10931093Regulatory protein SWI4PRO_0000067069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei255 – 2551Phosphoserine1 Publication
Modified residuei806 – 8061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25302.
PaxDbiP25302.

Expressioni

Gene expression databases

GenevestigatoriP25302.

Interactioni

Subunit structurei

Component of the transcription complex SCB-binding factor (SBF) composed of SWI6 and SWI4. Interacts with MSA2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-18626,EBI-18626
SWI6P099598EBI-18626,EBI-18641

Protein-protein interaction databases

BioGridi36856. 420 interactions.
DIPiDIP-847N.
IntActiP25302. 75 interactions.
MINTiMINT-698971.
STRINGi4932.YER111C.

Structurei

3D structure databases

ProteinModelPortaliP25302.
SMRiP25302. Positions 40-129, 469-678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 147111HTH APSES-typePROSITE-ProRule annotationAdd
BLAST
Repeati520 – 54930ANK 1Add
BLAST
Repeati641 – 67030ANK 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi201 – 727527Asn/Gln-richAdd
BLAST

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 HTH APSES-type DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00530000067565.
HOGENOMiHOG000141812.
InParanoidiP25302.
KOiK06649.
OMAiINTDING.
OrthoDBiEOG7WMCTB.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.10.260.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018004. KilA_N/APSES_HTH.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF04383. KilA-N. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF54616. SSF54616. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 2 hits.
PS51299. HTH_APSES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25302-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPFDVLISNQ KDNTNHQNIT PISKSVLLAP HSNHPVIEIA TYSETDVYEC
60 70 80 90 100
YIRGFETKIV MRRTKDDWIN ITQVFKIAQF SKTKRTKILE KESNDMQHEK
110 120 130 140 150
VQGGYGRFQG TWIPLDSAKF LVNKYEIIDP VVNSILTFQF DPNNPPPKRS
160 170 180 190 200
KNSILRKTSP GTKITSPSSY NKTPRKKNSS SSTSATTTAA NKKGKKNASI
210 220 230 240 250
NQPNPSPLQN LVFQTPQQFQ VNSSMNIMNN NDNHTTMNFN NDTRHNLINN
260 270 280 290 300
ISNNSNQSTI IQQQKSIHEN SFNNNYSATQ KPLQFFPIPT NLQNKNVALN
310 320 330 340 350
NPNNNDSNSY SHNIDNVINS SNNNNNGNNN NLIIVPDGPM QSQQQQQHHH
360 370 380 390 400
EYLTNNFNHS MMDSITNGNS KKRRKKLNQS NEQQFYNQQE KIQRHFKLMK
410 420 430 440 450
QPLLWQSFQN PNDHHNEYCD SNGSNNNNNT VASNGSSIEV FSSNENDNSM
460 470 480 490 500
NMSSRSMTPF SAGNTSSQNK LENKMTDQEY KQTILTILSS ERSSDVDQAL
510 520 530 540 550
LATLYPAPKN FNINFEIDDQ GHTPLHWATA MANIPLIKML ITLNANALQC
560 570 580 590 600
NKLGFNCITK SIFYNNCYKE NAFDEIISIL KICLITPDVN GRLPFHYLIE
610 620 630 640 650
LSVNKSKNPM IIKSYMDSII LSLGQQDYNL LKICLNYQDN IGNTPLHLSA
660 670 680 690 700
LNLNFEVYNR LVYLGASTDI LNLDNESPAS IMNKFNTPAG GSNSRNNNTK
710 720 730 740 750
ADRKLARNLP QKNYYQQQQQ QQQPQNNVKI PKIIKTQHPD KEDSTADVNI
760 770 780 790 800
AKTDSEVNES QYLHSNQPNS TNMNTIMEDL SNINSFVTSS VIKDIKSTPS
810 820 830 840 850
KILENSPILY RRRSQSISDE KEKAKDNENQ VEKKKDPLNS VKTAMPSLES
860 870 880 890 900
PSSLLPIQMS PLGKYSKPLS QQINKLNTKV SSLQRIMGEE IKNLDNEVVE
910 920 930 940 950
TESSISNNKK RLITIAHQIE DAFDSVSNKT PINSISDLQS RIKETSSKLN
960 970 980 990 1000
SEKQNFIQSL EKSQALKLAT IVQDEESKVD MNTNSSSHPE KQEDEEPIPK
1010 1020 1030 1040 1050
STSETSSPKN TKADAKFSNT VQESYDVNET LRLATELTIL QFKRRMTTLK
1060 1070 1080 1090
ISEAKSKINS SVKLDKYRNL IGITIENIDS KLDDIEKDLR ANA
Length:1,093
Mass (Da):123,806
Last modified:February 1, 1995 - v2
Checksum:i40EA2EA7732687A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1751R → T in CAA35949. (PubMed:2689885)Curated
Sequence conflicti431 – 4311V → I in CAA35949. (PubMed:2689885)Curated
Sequence conflicti1054 – 10541A → L in CAA35949. (PubMed:2689885)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51606 Genomic DNA. Translation: CAA35949.1.
U18916 Genomic DNA. Translation: AAC03209.1.
BK006939 Genomic DNA. Translation: DAA07771.1.
PIRiS50614.
RefSeqiNP_011036.1. NM_001179001.1.

Genome annotation databases

EnsemblFungiiYER111C; YER111C; YER111C.
GeneIDi856847.
KEGGisce:YER111C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51606 Genomic DNA. Translation: CAA35949.1 .
U18916 Genomic DNA. Translation: AAC03209.1 .
BK006939 Genomic DNA. Translation: DAA07771.1 .
PIRi S50614.
RefSeqi NP_011036.1. NM_001179001.1.

3D structure databases

ProteinModelPortali P25302.
SMRi P25302. Positions 40-129, 469-678.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36856. 420 interactions.
DIPi DIP-847N.
IntActi P25302. 75 interactions.
MINTi MINT-698971.
STRINGi 4932.YER111C.

Proteomic databases

MaxQBi P25302.
PaxDbi P25302.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER111C ; YER111C ; YER111C .
GeneIDi 856847.
KEGGi sce:YER111C.

Organism-specific databases

CYGDi YER111c.
SGDi S000000913. SWI4.

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00530000067565.
HOGENOMi HOG000141812.
InParanoidi P25302.
KOi K06649.
OMAi INTDING.
OrthoDBi EOG7WMCTB.

Enzyme and pathway databases

BioCyci YEAST:G3O-30275-MONOMER.

Miscellaneous databases

NextBioi 983174.

Gene expression databases

Genevestigatori P25302.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
3.10.260.10. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018004. KilA_N/APSES_HTH.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view ]
Pfami PF00023. Ank. 2 hits.
PF04383. KilA-N. 1 hit.
[Graphical view ]
SMARTi SM00248. ANK. 2 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF54616. SSF54616. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 2 hits.
PS51299. HTH_APSES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast SW14 protein contains a present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription."
    Andrews B.J., Herskowitz I.
    Nature 342:830-833(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast."
    Daniel J.
    Mol. Gen. Genet. 240:245-257(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 845-1093.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
    Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
    J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MSA2.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-255 AND SER-806, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSWI4_YEAST
AccessioniPrimary (citable) accession number: P25302
Secondary accession number(s): D3DM17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3