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P25300

- BUD5_YEAST

UniProt

P25300 - BUD5_YEAST

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Protein

Bud site selection protein 5

Gene

BUD5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GDP-GTP exchange factor (GEF) for the small GTPase BUD1/RSR1. Regulates the activity of BUD1 together with BUD2 which is a GTPase-activating protein (GAP) of BUD1. Required to produce both the axial and bipolar patterns of bud site selection. Determines the orientation of division axis. Overexpression can suppress mutations in PRP20 which is the GEF for GSP1. May be a cytoplasmic GEF for GSP1. Might also act on the Ras-like protein CDC42. Appears to bind to Ras proteins but not to activate them.3 Publications

GO - Molecular functioni

  1. Ras guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  1. axial cellular bud site selection Source: SGD
  2. bipolar cellular bud site selection Source: SGD
  3. positive regulation of Ras GTPase activity Source: GOC
  4. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-29350-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bud site selection protein 5
Gene namesi
Name:BUD5
Ordered Locus Names:YCR038C
ORF Names:YCR38C, YCR526
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR038c.
SGDiS000000634. BUD5.

Subcellular locationi

Bud neck. Cytoplasmcell cortex
Note: In haploid cells, forms double rings that encircle the mother-bud neck and split upon cytokinesis, each progeny cell inheriting BUD5 at the axial division remnant. In diploid cells, localizes to the poles of the cell.

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cytoplasm Source: UniProtKB-KW
  3. incipient cellular bud site Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 642642Bud site selection protein 5PRO_0000068859Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP25300.

Interactioni

Subunit structurei

Interacts with AXL2, BEM1, GSP1 and in haploid cells with AXL1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AXL2P389282EBI-3853,EBI-3397
GSP1P328352EBI-3853,EBI-7926

Protein-protein interaction databases

BioGridi31021. 16 interactions.
DIPiDIP-4991N.
IntActiP25300. 3 interactions.
MINTiMINT-474246.
STRINGi4932.YCR038C.

Structurei

3D structure databases

ProteinModelPortaliP25300.
SMRiP25300. Positions 411-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini224 – 339116N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini412 – 640229Ras-GEFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3417.
GeneTreeiENSGT00510000054299.
InParanoidiP25300.
OrthoDBiEOG7327XH.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25300-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSPKNKYVYI CVEYIYIYFA KIHKQSTLSS DTTKMFVLID NVLAYLLEQD
60 70 80 90 100
DLFVTARFAI QGQIVSRRVN KIHISNITDV LLQQFISHTL PYNDNIVPKK
110 120 130 140 150
ILDSMRTAVR QLLEATACVS RECPLVKRSQ DIKRARKRLL SDWYRLGADA
160 170 180 190 200
NMDAVLLVVN SAWRFLAVWR PFVNSIQHAT QELYQNIAHY LLHGNVNIQR
210 220 230 240 250
VTALIQLVMG QDDLLFSMDD VLQEVFRIQL YLNKMLPHNS HKWQKPSPFD
260 270 280 290 300
SANLLLNFRD WTTDNALLQE LLLSYPTINK NKHKNHSVPR LIQIWVESYW
310 320 330 340 350
QDSETTLKDI LNFWYSHLAE YYEYQELFAD IVQLFINKKR TRQLKIHYIG
360 370 380 390 400
LTDKEIEENK PPLDYENLFL QYEIDKTNAN DELCGATDLS DLLFQWKQGE
410 420 430 440 450
LLEVEAFALN VSPWSLAKTL TLLESSLYLD IETIEFTRHF KHNDTTIDSV
460 470 480 490 500
FTLSNQLSSY VLETTLQQTH TISYWLQVAL SCLYLRNLNS LASIITSLQN
510 520 530 540 550
HSIERLSLPI DVKSDHLFQR LKVVVHPNNN YNVYRRTIKH IFHSQLPCVP
560 570 580 590 600
FTSLLIRDIT FIRDGNDTFT KDGNNVNMQK FNQITKIVAF AQYLQQKQYE
610 620 630 640
DIHCSNTTAR SLLGAMIKVH TLYNDNKDRA YQVSIAKVPR LT
Length:642
Mass (Da):75,046
Last modified:March 15, 2004 - v2
Checksum:i55E688DF5AFAB74F
GO

Sequence cautioni

The sequence AAA34462.1 differs from that shown. Reason: Frameshift at positions 60 and 62. Curated
The sequence CAA45334.1 differs from that shown. Reason: Frameshift at position 38. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101R → P in CAA45334. (PubMed:1789011)Curated
Sequence conflicti111 – 1111Missing in AAA34462. (PubMed:1905982)Curated
Sequence conflicti225 – 2251V → D in AAA34460. (PubMed:1905981)Curated
Sequence conflicti401 – 4011L → P in AAA34462. (PubMed:1905982)Curated
Sequence conflicti401 – 4011L → P in CAA40230. (PubMed:1964349)Curated
Sequence conflicti481 – 4811S → A in AAA34462. (PubMed:1905982)Curated
Sequence conflicti481 – 4811S → A in CAA40230. (PubMed:1964349)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63853 Genomic DNA. Translation: CAA45334.1. Frameshift.
M68938 Genomic DNA. Translation: AAA34462.1. Frameshift.
X59720 Genomic DNA. Translation: CAA42305.2.
M63552 Genomic DNA. Translation: AAA34460.1.
X56909 Genomic DNA. Translation: CAA40230.1.
BK006937 Genomic DNA. Translation: DAA07517.1.
PIRiS19450. BWBYD5.
RefSeqiNP_009967.2. NM_001178752.1.

Genome annotation databases

EnsemblFungiiYCR038C; YCR038C; YCR038C.
GeneIDi850405.
KEGGisce:YCR038C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63853 Genomic DNA. Translation: CAA45334.1 . Frameshift.
M68938 Genomic DNA. Translation: AAA34462.1 . Frameshift.
X59720 Genomic DNA. Translation: CAA42305.2 .
M63552 Genomic DNA. Translation: AAA34460.1 .
X56909 Genomic DNA. Translation: CAA40230.1 .
BK006937 Genomic DNA. Translation: DAA07517.1 .
PIRi S19450. BWBYD5.
RefSeqi NP_009967.2. NM_001178752.1.

3D structure databases

ProteinModelPortali P25300.
SMRi P25300. Positions 411-599.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31021. 16 interactions.
DIPi DIP-4991N.
IntActi P25300. 3 interactions.
MINTi MINT-474246.
STRINGi 4932.YCR038C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR038C ; YCR038C ; YCR038C .
GeneIDi 850405.
KEGGi sce:YCR038C.

Organism-specific databases

CYGDi YCR038c.
SGDi S000000634. BUD5.

Phylogenomic databases

eggNOGi KOG3417.
GeneTreei ENSGT00510000054299.
InParanoidi P25300.
OrthoDBi EOG7327XH.

Enzyme and pathway databases

BioCyci YEAST:G3O-29350-MONOMER.

Miscellaneous databases

NextBioi 965947.

Gene expression databases

Genevestigatori P25300.

Family and domain databases

Gene3Di 1.10.840.10. 1 hit.
InterProi IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
[Graphical view ]
Pfami PF00617. RasGEF. 1 hit.
[Graphical view ]
SMARTi SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view ]
SUPFAMi SSF48366. SSF48366. 1 hit.
PROSITEi PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames."
    Jacquet M., Buhler J.-M., Iborra F., Francingues-Gaillard M.-C., Soustelle C.
    Yeast 7:881-888(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant."
    Powers S., Gonzales E., Christensen T., Cubert J., Broek D.
    Cell 65:1225-1231(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Valles G., Volckaerts G.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 110; 401; 481 AND N-TERMINUS.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1."
    Chant J., Corrado K., Pringle J.R., Herskowitz I.
    Cell 65:1213-1224(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 105-642.
    Strain: SP1.
  7. "The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase."
    Thierry A., Fairhead C., Dujon B.
    Yeast 6:521-534(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 287-642.
    Strain: ATCC 96604 / S288c / FY1679.
  8. "Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast."
    Bender A.
    Proc. Natl. Acad. Sci. U.S.A. 90:9926-9929(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "A localized GTPase exchange factor, Bud5, determines the orientation of division axes in yeast."
    Marston A.L., Chen T., Yang M.C., Belhumeur P., Chant J.
    Curr. Biol. 11:803-807(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae."
    Clement M., Lavallee F., Barbes-Morin G., de Repentigny L., Belhumeur P.
    Mol. Genet. Genomics 266:20-27(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GSP1.
  11. "A GDP/GTP exchange factor involved in linking a spatial landmark to cell polarity."
    Kang P.J., Sanson A., Lee B., Park H.-O.
    Science 292:1376-1378(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AXL2.
  12. "Subcellular localization of Axl1, the cell type-specific regulator of polarity."
    Lord M., Inose F., Hiroko T., Hata T., Fujita A., Chant J.
    Curr. Biol. 12:1347-1352(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AXL1.
  13. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  14. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBUD5_YEAST
AccessioniPrimary (citable) accession number: P25300
Secondary accession number(s): D6VR48, Q06729
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: March 15, 2004
Last modified: October 29, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2110 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3