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Protein

Bud site selection protein 5

Gene

BUD5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GDP-GTP exchange factor (GEF) for the small GTPase BUD1/RSR1. Regulates the activity of BUD1 together with BUD2 which is a GTPase-activating protein (GAP) of BUD1. Required to produce both the axial and bipolar patterns of bud site selection. Determines the orientation of division axis. Overexpression can suppress mutations in PRP20 which is the GEF for GSP1. May be a cytoplasmic GEF for GSP1. Might also act on the Ras-like protein CDC42. Appears to bind to Ras proteins but not to activate them.3 Publications

Miscellaneous

Present with 2110 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • Ras guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  • axial cellular bud site selection Source: SGD
  • bipolar cellular bud site selection Source: SGD
  • small GTPase mediated signal transduction Source: InterPro

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-29350-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bud site selection protein 5
Gene namesi
Name:BUD5
Ordered Locus Names:YCR038C
ORF Names:YCR38C, YCR526
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR038C
SGDiS000000634 BUD5

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688591 – 642Bud site selection protein 5Add BLAST642

Proteomic databases

PaxDbiP25300
PRIDEiP25300

Interactioni

Subunit structurei

Interacts with AXL2, BEM1, GSP1 and in haploid cells with AXL1.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • Ras guanyl-nucleotide exchange factor activity Source: SGD

Protein-protein interaction databases

BioGridi31021, 18 interactors
DIPiDIP-4991N
IntActiP25300, 8 interactors
MINTiP25300
STRINGi4932.YCR038C

Structurei

3D structure databases

ProteinModelPortaliP25300
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 339N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST116
Domaini412 – 640Ras-GEFPROSITE-ProRule annotationAdd BLAST229

Phylogenomic databases

GeneTreeiENSGT00510000054299
InParanoidiP25300
OrthoDBiEOG092C10HX

Family and domain databases

CDDicd00155 RasGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
PfamiView protein in Pfam
PF00617 RasGEF, 1 hit
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P25300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPKNKYVYI CVEYIYIYFA KIHKQSTLSS DTTKMFVLID NVLAYLLEQD
60 70 80 90 100
DLFVTARFAI QGQIVSRRVN KIHISNITDV LLQQFISHTL PYNDNIVPKK
110 120 130 140 150
ILDSMRTAVR QLLEATACVS RECPLVKRSQ DIKRARKRLL SDWYRLGADA
160 170 180 190 200
NMDAVLLVVN SAWRFLAVWR PFVNSIQHAT QELYQNIAHY LLHGNVNIQR
210 220 230 240 250
VTALIQLVMG QDDLLFSMDD VLQEVFRIQL YLNKMLPHNS HKWQKPSPFD
260 270 280 290 300
SANLLLNFRD WTTDNALLQE LLLSYPTINK NKHKNHSVPR LIQIWVESYW
310 320 330 340 350
QDSETTLKDI LNFWYSHLAE YYEYQELFAD IVQLFINKKR TRQLKIHYIG
360 370 380 390 400
LTDKEIEENK PPLDYENLFL QYEIDKTNAN DELCGATDLS DLLFQWKQGE
410 420 430 440 450
LLEVEAFALN VSPWSLAKTL TLLESSLYLD IETIEFTRHF KHNDTTIDSV
460 470 480 490 500
FTLSNQLSSY VLETTLQQTH TISYWLQVAL SCLYLRNLNS LASIITSLQN
510 520 530 540 550
HSIERLSLPI DVKSDHLFQR LKVVVHPNNN YNVYRRTIKH IFHSQLPCVP
560 570 580 590 600
FTSLLIRDIT FIRDGNDTFT KDGNNVNMQK FNQITKIVAF AQYLQQKQYE
610 620 630 640
DIHCSNTTAR SLLGAMIKVH TLYNDNKDRA YQVSIAKVPR LT
Length:642
Mass (Da):75,046
Last modified:March 15, 2004 - v2
Checksum:i55E688DF5AFAB74F
GO

Sequence cautioni

The sequence AAA34462 differs from that shown. Reason: Frameshift at positions 60 and 62.Curated
The sequence CAA45334 differs from that shown. Reason: Frameshift at position 38.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110R → P in CAA45334 (PubMed:1789011).Curated1
Sequence conflicti111Missing in AAA34462 (PubMed:1905982).Curated1
Sequence conflicti225V → D in AAA34460 (PubMed:1905981).Curated1
Sequence conflicti401L → P in AAA34462 (PubMed:1905982).Curated1
Sequence conflicti401L → P in CAA40230 (PubMed:1964349).Curated1
Sequence conflicti481S → A in AAA34462 (PubMed:1905982).Curated1
Sequence conflicti481S → A in CAA40230 (PubMed:1964349).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63853 Genomic DNA Translation: CAA45334.1 Frameshift.
M68938 Genomic DNA Translation: AAA34462.1 Frameshift.
X59720 Genomic DNA Translation: CAA42305.2
M63552 Genomic DNA Translation: AAA34460.1
X56909 Genomic DNA Translation: CAA40230.1
BK006937 Genomic DNA Translation: DAA07517.1
PIRiS19450 BWBYD5
RefSeqiNP_009967.2, NM_001178752.1

Genome annotation databases

EnsemblFungiiCAA42305; CAA42305; CAA42305
YCR038C; YCR038C; YCR038C
GeneIDi850405
KEGGisce:YCR038C

Similar proteinsi

Entry informationi

Entry nameiBUD5_YEAST
AccessioniPrimary (citable) accession number: P25300
Secondary accession number(s): D6VR48, Q06729
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: March 15, 2004
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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