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P25300 (BUD5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bud site selection protein 5
Gene names
Name:BUD5
Ordered Locus Names:YCR038C
ORF Names:YCR38C, YCR526
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length642 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GDP-GTP exchange factor (GEF) for the small GTPase BUD1/RSR1. Regulates the activity of BUD1 together with BUD2 which is a GTPase-activating protein (GAP) of BUD1. Required to produce both the axial and bipolar patterns of bud site selection. Determines the orientation of division axis. Overexpression can suppress mutations in PRP20 which is the GEF for GSP1. May be a cytoplasmic GEF for GSP1. Might also act on the Ras-like protein CDC42. Appears to bind to Ras proteins but not to activate them. Ref.8 Ref.9 Ref.10

Subunit structure

Interacts with AXL2, BEM1, GSP1 and in haploid cells with AXL1. Ref.10 Ref.11 Ref.12

Subcellular location

Bud neck. Cytoplasmcell cortex. Note: In haploid cells, forms double rings that encircle the mother-bud neck and split upon cytokinesis, each progeny cell inheriting BUD5 at the axial division remnant. In diploid cells, localizes to the poles of the cell. Ref.9 Ref.11 Ref.12 Ref.13

Miscellaneous

Present with 2110 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 N-terminal Ras-GEF domain.

Contains 1 Ras-GEF domain.

Sequence caution

The sequence AAA34462.1 differs from that shown. Reason: Frameshift at positions 60 and 62.

The sequence CAA45334.1 differs from that shown. Reason: Frameshift at position 38.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AXL2P389282EBI-3853,EBI-3397
GSP1P328352EBI-3853,EBI-7926

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 642642Bud site selection protein 5
PRO_0000068859

Regions

Domain224 – 339116N-terminal Ras-GEF
Domain412 – 640229Ras-GEF

Experimental info

Sequence conflict1101R → P in CAA45334. Ref.1
Sequence conflict1111Missing in AAA34462. Ref.2
Sequence conflict2251V → D in AAA34460. Ref.6
Sequence conflict4011L → P in AAA34462. Ref.2
Sequence conflict4011L → P in CAA40230. Ref.7
Sequence conflict4811S → A in AAA34462. Ref.2
Sequence conflict4811S → A in CAA40230. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P25300 [UniParc].

Last modified March 15, 2004. Version 2.
Checksum: 55E688DF5AFAB74F

FASTA64275,046
        10         20         30         40         50         60 
MSPKNKYVYI CVEYIYIYFA KIHKQSTLSS DTTKMFVLID NVLAYLLEQD DLFVTARFAI 

        70         80         90        100        110        120 
QGQIVSRRVN KIHISNITDV LLQQFISHTL PYNDNIVPKK ILDSMRTAVR QLLEATACVS 

       130        140        150        160        170        180 
RECPLVKRSQ DIKRARKRLL SDWYRLGADA NMDAVLLVVN SAWRFLAVWR PFVNSIQHAT 

       190        200        210        220        230        240 
QELYQNIAHY LLHGNVNIQR VTALIQLVMG QDDLLFSMDD VLQEVFRIQL YLNKMLPHNS 

       250        260        270        280        290        300 
HKWQKPSPFD SANLLLNFRD WTTDNALLQE LLLSYPTINK NKHKNHSVPR LIQIWVESYW 

       310        320        330        340        350        360 
QDSETTLKDI LNFWYSHLAE YYEYQELFAD IVQLFINKKR TRQLKIHYIG LTDKEIEENK 

       370        380        390        400        410        420 
PPLDYENLFL QYEIDKTNAN DELCGATDLS DLLFQWKQGE LLEVEAFALN VSPWSLAKTL 

       430        440        450        460        470        480 
TLLESSLYLD IETIEFTRHF KHNDTTIDSV FTLSNQLSSY VLETTLQQTH TISYWLQVAL 

       490        500        510        520        530        540 
SCLYLRNLNS LASIITSLQN HSIERLSLPI DVKSDHLFQR LKVVVHPNNN YNVYRRTIKH 

       550        560        570        580        590        600 
IFHSQLPCVP FTSLLIRDIT FIRDGNDTFT KDGNNVNMQK FNQITKIVAF AQYLQQKQYE 

       610        620        630        640 
DIHCSNTTAR SLLGAMIKVH TLYNDNKDRA YQVSIAKVPR LT 

« Hide

References

« Hide 'large scale' references
[1]"The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames."
Jacquet M., Buhler J.-M., Iborra F., Francingues-Gaillard M.-C., Soustelle C.
Yeast 7:881-888(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant."
Powers S., Gonzales E., Christensen T., Cubert J., Broek D.
Cell 65:1225-1231(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 110; 401; 481 AND N-TERMINUS.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1."
Chant J., Corrado K., Pringle J.R., Herskowitz I.
Cell 65:1213-1224(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 105-642.
Strain: SP1.
[7]"The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase."
Thierry A., Fairhead C., Dujon B.
Yeast 6:521-534(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 287-642.
Strain: ATCC 96604 / S288c / FY1679.
[8]"Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast."
Bender A.
Proc. Natl. Acad. Sci. U.S.A. 90:9926-9929(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"A localized GTPase exchange factor, Bud5, determines the orientation of division axes in yeast."
Marston A.L., Chen T., Yang M.C., Belhumeur P., Chant J.
Curr. Biol. 11:803-807(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae."
Clement M., Lavallee F., Barbes-Morin G., de Repentigny L., Belhumeur P.
Mol. Genet. Genomics 266:20-27(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GSP1.
[11]"A GDP/GTP exchange factor involved in linking a spatial landmark to cell polarity."
Kang P.J., Sanson A., Lee B., Park H.-O.
Science 292:1376-1378(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AXL2.
[12]"Subcellular localization of Axl1, the cell type-specific regulator of polarity."
Lord M., Inose F., Hiroko T., Hata T., Fujita A., Chant J.
Curr. Biol. 12:1347-1352(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AXL1.
[13]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[14]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63853 Genomic DNA. Translation: CAA45334.1. Frameshift.
M68938 Genomic DNA. Translation: AAA34462.1. Frameshift.
X59720 Genomic DNA. Translation: CAA42305.2.
M63552 Genomic DNA. Translation: AAA34460.1.
X56909 Genomic DNA. Translation: CAA40230.1.
BK006937 Genomic DNA. Translation: DAA07517.1.
PIRBWBYD5. S19450.
RefSeqNP_009967.2. NM_001178752.1.

3D structure databases

ProteinModelPortalP25300.
SMRP25300. Positions 411-599.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31021. 16 interactions.
DIPDIP-4991N.
IntActP25300. 3 interactions.
MINTMINT-474246.
STRING4932.YCR038C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR038C; YCR038C; YCR038C.
GeneID850405.
KEGGsce:YCR038C.

Organism-specific databases

CYGDYCR038c.
SGDS000000634. BUD5.

Phylogenomic databases

eggNOGKOG3417.
GeneTreeENSGT00510000054299.
OrthoDBEOG7327XH.

Enzyme and pathway databases

BioCycYEAST:G3O-29350-MONOMER.

Gene expression databases

GenevestigatorP25300.

Family and domain databases

Gene3D1.10.840.10. 1 hit.
InterProIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
[Graphical view]
PfamPF00617. RasGEF. 1 hit.
[Graphical view]
SMARTSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMSSF48366. SSF48366. 1 hit.
PROSITEPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965947.

Entry information

Entry nameBUD5_YEAST
AccessionPrimary (citable) accession number: P25300
Secondary accession number(s): D6VR48, Q06729
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: March 15, 2004
Last modified: June 11, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families