Reviewed, UniProtKB/Swiss-Prot P25300 (BUD5_YEAST)

Names and origin

Protein names

Recommended name:
    Bud site selection protein 5

Gene names

Name:	BUD5
Ordered Locus Names:	YCR038C
ORF Names:	YCR38C, YCR526

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GDP-GTP exchange factor (GEF) for the small GTPase BUD1/RSR1. Regulates the activity of BUD1 together with BUD2 which is a GTPase-activating protein (GAP) of BUD1. Required to produce both the axial and bipolar patterns of bud site selection. Determines the orientation of division axis. Overexpression can suppress mutations in PRP20 which is the GEF for GSP1. May be a cytoplasmic GEF for GSP1. Might also act on the Ras-like protein CDC42. Appears to bind to Ras proteins but not to activate them. Ref.7 Ref.8 Ref.9
Subunit structure	Interacts with AXL2, BEM1, GSP1 and in haploid cells with AXL1. Ref.9 Ref.10 Ref.11
Subcellular location	Bud neck. Cytoplasm › cell cortex. Note: In haploid cells, forms double rings that encircle the mother-bud neck and split upon cytokinesis, each progeny cell inheriting BUD5 at the axial division remnant. In diploid cells, localizes to the poles of the cell. Ref.8 Ref.10 Ref.11 Ref.12
Miscellaneous	Present with 2110 molecules/cell in log phase SD medium. Ref.13
Sequence similarities	Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain.
Sequence caution	The sequence AAA34462.1 differs from that shown. Reason: Frameshift at positions 60 and 62. The sequence CAA45334.1 differs from that shown. Reason: Frameshift at position 38.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	axial cellular bud site selection Ref.5 Traceable author statement. Source: SGD bipolar cellular bud site selection Ref.5 Traceable author statement. Source: SGD pseudohyphal growth Traceable author statement. Source: SGD regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Traceable author statement. Source: SGD
Cellular component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud neck Ref.10 Inferred from direct assay. Source: SGD incipient cellular bud site Ref.10 Inferred from direct assay. Source: SGD
Molecular function	Ras guanyl-nucleotide exchange factor activity Traceable author statement. Source: SGD protein binding Ref.9 Ref.10 Ref.11 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AXL1	P40851	1	EBI-3853,EBI-3387
AXL2	P38928	2	EBI-3853,EBI-3397
GSP1	P32835	2	EBI-3853,EBI-7926

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	Bud site selection protein 5		PRO_0000068859
Regions
	Domain	224 – 339	116	N-terminal Ras-GEF
	Domain	412 – 640	229	Ras-GEF
Experimental info
	Sequence conflict	110	1	R → P in CAA45334. Ref.1
	Sequence conflict	111	1	Missing in AAA34462. Ref.2
	Sequence conflict	225	1	V → D in AAA34460. Ref.5
	Sequence conflict	401	1	L → P Ref.2
	Sequence conflict	401	1	L → P Ref.6
	Sequence conflict	481	1	S → A Ref.2
	Sequence conflict	481	1	S → A Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P25300-1 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: 55E688DF5AFAB74F	FASTA	642	75,046
	10 20 30 40 50 60 MSPKNKYVYI CVEYIYIYFA KIHKQSTLSS DTTKMFVLID NVLAYLLEQD DLFVTARFAI 70 80 90 100 110 120 QGQIVSRRVN KIHISNITDV LLQQFISHTL PYNDNIVPKK ILDSMRTAVR QLLEATACVS 130 140 150 160 170 180 RECPLVKRSQ DIKRARKRLL SDWYRLGADA NMDAVLLVVN SAWRFLAVWR PFVNSIQHAT 190 200 210 220 230 240 QELYQNIAHY LLHGNVNIQR VTALIQLVMG QDDLLFSMDD VLQEVFRIQL YLNKMLPHNS 250 260 270 280 290 300 HKWQKPSPFD SANLLLNFRD WTTDNALLQE LLLSYPTINK NKHKNHSVPR LIQIWVESYW 310 320 330 340 350 360 QDSETTLKDI LNFWYSHLAE YYEYQELFAD IVQLFINKKR TRQLKIHYIG LTDKEIEENK 370 380 390 400 410 420 PPLDYENLFL QYEIDKTNAN DELCGATDLS DLLFQWKQGE LLEVEAFALN VSPWSLAKTL 430 440 450 460 470 480 TLLESSLYLD IETIEFTRHF KHNDTTIDSV FTLSNQLSSY VLETTLQQTH TISYWLQVAL 490 500 510 520 530 540 SCLYLRNLNS LASIITSLQN HSIERLSLPI DVKSDHLFQR LKVVVHPNNN YNVYRRTIKH 550 560 570 580 590 600 IFHSQLPCVP FTSLLIRDIT FIRDGNDTFT KDGNNVNMQK FNQITKIVAF AQYLQQKQYE 610 620 630 640 DIHCSNTTAR SLLGAMIKVH TLYNDNKDRA YQVSIAKVPR LT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames." Jacquet M., Buhler J.-M., Iborra F., Francingues-Gaillard M.-C., Soustelle C. Yeast 7:881-888(1991) [PubMed: 1789011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant." Powers S., Gonzales E., Christensen T., Cubert J., Broek D. Cell 65:1225-1231(1991) [PubMed: 1905982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Valles G., Volckaerts G. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 110; 401; 481 AND N-TERMINUS.
[5]	"Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1." Chant J., Corrado K., Pringle J.R., Herskowitz I. Cell 65:1213-1224(1991) [PubMed: 1905981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 105-642. Strain: SP1.
[6]	"The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase." Thierry A., Fairhead C., Dujon B. Yeast 6:521-534(1990) [PubMed: 1964349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 287-642. Strain: ATCC 96604 / S288c / FY1679.
[7]	"Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast." Bender A. Proc. Natl. Acad. Sci. U.S.A. 90:9926-9929(1993) [PubMed: 8234337] [Abstract] Cited for: FUNCTION.
[8]	"A localized GTPase exchange factor, Bud5, determines the orientation of division axes in yeast." Marston A.L., Chen T., Yang M.C., Belhumeur P., Chant J. Curr. Biol. 11:803-807(2001) [PubMed: 11378394] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae." Clement M., Lavallee F., Barbes-Morin G., de Repentigny L., Belhumeur P. Mol. Genet. Genomics 266:20-27(2001) [PubMed: 11589573] [Abstract] Cited for: FUNCTION, INTERACTION WITH GSP1.
[10]	"A GDP/GTP exchange factor involved in linking a spatial landmark to cell polarity." Kang P.J., Sanson A., Lee B., Park H.-O. Science 292:1376-1378(2001) [PubMed: 11313501] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AXL2.
[11]	"Subcellular localization of Axl1, the cell type-specific regulator of polarity." Lord M., Inose F., Hiroko T., Hata T., Fujita A., Chant J. Curr. Biol. 12:1347-1352(2002) [PubMed: 12176366] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AXL1.
[12]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63853 Genomic DNA. Translation: CAA45334.1. Frameshift. M68938 Genomic DNA. Translation: AAA34462.1. Frameshift. X59720 Genomic DNA. Translation: CAA42305.2. M63552 Genomic DNA. Translation: AAA34460.1. X56909 Genomic DNA. Translation: CAA40230.1.
PIR	BWBYD5. S19450.
RefSeq	NP_009967.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4991N.
IntAct	P25300. 8 interactions.
Genome annotation databases
Ensembl	YCR038C. Saccharomyces cerevisiae. [Contig view]
GeneID	850405.
GenomeReviews	Gene locus YCR038C in contig X59720_GR.
KEGG	sce:YCR038C.
NMPDR	fig|4932.3.peg.692.
Organism-specific databases
CYGD	YCR038c.
SGD	S000000634. BUD5.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P25300.
Gene expression databases
GermOnline	YCR038C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019804. Ras_G-nucl-exch_fac_CS. IPR008937. Ras_GEF. IPR000651. RasGef_N. IPR001895. RasGRF_CDC25. [Graphical view]
Gene3D	G3DSA:1.10.840.10. RasGRF_CDC25. 1 hit.
PANTHER	PTHR23113. Ras_GEF. 1 hit.
Pfam	PF00617. RasGEF. 1 hit. [Graphical view]
SMART	SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. [Graphical view]
PROSITE	PS00720. RASGEF. 1 hit. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	965947.

Entry information

Entry name

BUD5_YEAST

Accession

Primary (citable) accession number: P25300
Secondary accession number(s): Q06729

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	March 15, 2004
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)