Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

mRNA 3'-end-processing protein RNA15

Gene

RNA15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Binds to A-rich RNA sequence elements.2 Publications

GO - Molecular functioni

  • mRNA binding Source: SGD
  • nucleotide binding Source: InterPro
  • protein heterodimerization activity Source: SGD

GO - Biological processi

  • mRNA polyadenylation Source: SGD
  • pre-mRNA cleavage required for polyadenylation Source: SGD
  • response to DNA damage checkpoint signaling Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30555-MONOMER.
ReactomeiR-SCE-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA 3'-end-processing protein RNA15
Gene namesi
Name:RNA15
Ordered Locus Names:YGL044C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL044C.
SGDiS000003012. RNA15.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818071 – 296mRNA 3'-end-processing protein RNA15Add BLAST296

Proteomic databases

MaxQBiP25299.
PRIDEiP25299.

Interactioni

Subunit structurei

Component of the CFIA complex, which is composed of RNA14, RNA15, PCF11 and CLP1. Interacts directly with RNA14. Interacts with polyadenylate-binding protein PAB1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PCF11P390819EBI-15640,EBI-12980
RNA14P252988EBI-15640,EBI-15632
SUB1P540002EBI-15640,EBI-18492

GO - Molecular functioni

  • protein heterodimerization activity Source: SGD

Protein-protein interaction databases

BioGridi33204. 119 interactors.
DIPiDIP-1489N.
IntActiP25299. 11 interactors.
MINTiMINT-389896.

Structurei

Secondary structure

1296
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 24Combined sources7
Helixi31 – 39Combined sources9
Beta strandi44 – 48Combined sources5
Turni53 – 55Combined sources3
Beta strandi60 – 68Combined sources9
Helixi69 – 79Combined sources11
Beta strandi85 – 87Combined sources3
Beta strandi90 – 93Combined sources4
Helixi99 – 101Combined sources3
Helixi139 – 143Combined sources5
Turni156 – 158Combined sources3
Helixi161 – 170Combined sources10
Helixi174 – 190Combined sources17
Helixi192 – 201Combined sources10
Helixi203 – 216Combined sources14
Beta strandi217 – 219Combined sources3
Helixi221 – 224Combined sources4
Turni225 – 227Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KM8NMR-B14-97[»]
2L9BNMR-A127-232[»]
2X1AX-ray2.05A16-111[»]
2X1BX-ray1.80A16-111[»]
2X1FX-ray1.60A16-103[»]
ProteinModelPortaliP25299.
SMRiP25299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25299.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 96RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi104 – 109Poly-Gln6
Compositional biasi111 – 128Asn-richAdd BLAST18

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00820000127074.
HOGENOMiHOG000247912.
KOiK14407.
OMAiNINMTTP.
OrthoDBiEOG092C3SCB.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRQSGVNAG VQNNPPSRVV YLGSIPYDQT EEQILDLCSN VGPVINLKMM
60 70 80 90 100
FDPQTGRSKG YAFIEFRDLE SSASAVRNLN GYQLGSRFLK CGYSSNSDIS
110 120 130 140 150
GVSQQQQQQY NNINGNNNNN GNNNNNSNGP DFQNSGNANF LSQKFPELPS
160 170 180 190 200
GIDVNINMTT PAMMISSELA KKPKEVQLKF LQKFQEWTRA HPEDAVSLLE
210 220 230 240 250
LCPQLSFVTA ELLLTNGICK VDDLIPLASR PQEEASATNN NSVNEVVDPA
260 270 280 290
VLNKQKELLK QVLQLNDSQI SILPDDERMA IWDLKQKALR GEFGAF
Length:296
Mass (Da):32,791
Last modified:May 1, 1992 - v1
Checksum:i10053B8648A71ECB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73462 Genomic DNA. Translation: AAA34984.1.
Z72566 Genomic DNA. Translation: CAA96746.1.
BK006941 Genomic DNA. Translation: DAA08056.1.
PIRiB40257.
RefSeqiNP_011471.1. NM_001180909.1.

Genome annotation databases

EnsemblFungiiYGL044C; YGL044C; YGL044C.
GeneIDi852838.
KEGGisce:YGL044C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73462 Genomic DNA. Translation: AAA34984.1.
Z72566 Genomic DNA. Translation: CAA96746.1.
BK006941 Genomic DNA. Translation: DAA08056.1.
PIRiB40257.
RefSeqiNP_011471.1. NM_001180909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KM8NMR-B14-97[»]
2L9BNMR-A127-232[»]
2X1AX-ray2.05A16-111[»]
2X1BX-ray1.80A16-111[»]
2X1FX-ray1.60A16-103[»]
ProteinModelPortaliP25299.
SMRiP25299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33204. 119 interactors.
DIPiDIP-1489N.
IntActiP25299. 11 interactors.
MINTiMINT-389896.

Proteomic databases

MaxQBiP25299.
PRIDEiP25299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL044C; YGL044C; YGL044C.
GeneIDi852838.
KEGGisce:YGL044C.

Organism-specific databases

EuPathDBiFungiDB:YGL044C.
SGDiS000003012. RNA15.

Phylogenomic databases

GeneTreeiENSGT00820000127074.
HOGENOMiHOG000247912.
KOiK14407.
OMAiNINMTTP.
OrthoDBiEOG092C3SCB.

Enzyme and pathway databases

BioCyciYEAST:G3O-30555-MONOMER.
ReactomeiR-SCE-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

EvolutionaryTraceiP25299.
PROiP25299.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNA15_YEAST
AccessioniPrimary (citable) accession number: P25299
Secondary accession number(s): D6VU95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6350 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.