Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P25299 (RNA15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
mRNA 3'-end-processing protein RNA15
Gene names
Name:RNA15
Ordered Locus Names:YGL044C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Binds to A-rich RNA sequence elements. Ref.5 Ref.7

Subunit structure

Component of the CFIA complex, which is composed of RNA14, RNA15, PCF11 and CLP1. Interacts directly with RNA14. Interacts with polyadenylate-binding protein PAB1. Ref.6

Subcellular location

Nucleus.

Miscellaneous

Present with 6350 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296mRNA 3'-end-processing protein RNA15
PRO_0000081807

Regions

Domain18 – 9679RRM
Compositional bias104 – 1096Poly-Gln
Compositional bias111 – 12818Asn-rich

Secondary structure

.................................. 296
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25299 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 10053B8648A71ECB

FASTA29632,791
        10         20         30         40         50         60 
MNRQSGVNAG VQNNPPSRVV YLGSIPYDQT EEQILDLCSN VGPVINLKMM FDPQTGRSKG 

        70         80         90        100        110        120 
YAFIEFRDLE SSASAVRNLN GYQLGSRFLK CGYSSNSDIS GVSQQQQQQY NNINGNNNNN 

       130        140        150        160        170        180 
GNNNNNSNGP DFQNSGNANF LSQKFPELPS GIDVNINMTT PAMMISSELA KKPKEVQLKF 

       190        200        210        220        230        240 
LQKFQEWTRA HPEDAVSLLE LCPQLSFVTA ELLLTNGICK VDDLIPLASR PQEEASATNN 

       250        260        270        280        290 
NSVNEVVDPA VLNKQKELLK QVLQLNDSQI SILPDDERMA IWDLKQKALR GEFGAF 

« Hide

References

« Hide 'large scale' references
[1]"Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein."
Minvielle-Sebastia L., Winsor B., Bonneaud N., Lacroute F.
Mol. Cell. Biol. 11:3075-3087(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
Feuermann M., de Montigny J., Potier S., Souciet J.-L.
Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"RNA14 and RNA15 proteins as components of a yeast pre-mRNA 3'-end processing factor."
Minvielle-Sebastia L., Preker P.J., Keller W.
Science 266:1702-1705(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro."
Amrani N., Minet M., Le Gouar M., Lacroute F., Wyers F.
Mol. Cell. Biol. 17:3694-3701(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PAB1.
[7]"Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I."
Gross S., Moore C.
Proc. Natl. Acad. Sci. U.S.A. 98:6080-6085(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE CFIA COMPLEX.
[8]"Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation."
Gross S., Moore C.L.
Mol. Cell. Biol. 21:8045-8055(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA-BINDING.
[9]"Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends."
Kessler M.M., Zhao J., Moore C.L.
J. Biol. Chem. 271:27167-27175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF THE CFIA COMPLEX.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M73462 Genomic DNA. Translation: AAA34984.1.
Z72566 Genomic DNA. Translation: CAA96746.1.
BK006941 Genomic DNA. Translation: DAA08056.1.
PIRB40257.
RefSeqNP_011471.1. NM_001180909.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KM8NMR-B14-97[»]
2L9BNMR-A127-232[»]
2X1AX-ray2.05A16-111[»]
2X1BX-ray1.80A16-111[»]
2X1FX-ray1.60A16-103[»]
ProteinModelPortalP25299.
SMRP25299. Positions 16-101, 138-228, 250-295.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33204. 114 interactions.
DIPDIP-1489N.
IntActP25299. 11 interactions.
MINTMINT-389896.
STRING4932.YGL044C.

Proteomic databases

PaxDbP25299.
PeptideAtlasP25299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL044C; YGL044C; YGL044C.
GeneID852838.
KEGGsce:YGL044C.

Organism-specific databases

CYGDYGL044c.
SGDS000003012. RNA15.

Phylogenomic databases

eggNOGNOG246793.
GeneTreeENSGT00750000117707.
HOGENOMHOG000247912.
KOK14407.
OMAQILDLCS.
OrthoDBEOG706125.

Enzyme and pathway databases

BioCycYEAST:G3O-30555-MONOMER.

Gene expression databases

GenevestigatorP25299.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP25299.
NextBio972414.

Entry information

Entry nameRNA15_YEAST
AccessionPrimary (citable) accession number: P25299
Secondary accession number(s): D6VU95
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references