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P25299

- RNA15_YEAST

UniProt

P25299 - RNA15_YEAST

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Protein

mRNA 3'-end-processing protein RNA15

Gene

RNA15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

RNA-binding component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Binds to A-rich RNA sequence elements.2 Publications

GO - Molecular functioni

  1. mRNA binding Source: SGD
  2. nucleotide binding Source: InterPro
  3. protein heterodimerization activity Source: SGD

GO - Biological processi

  1. mRNA cleavage Source: SGD
  2. mRNA polyadenylation Source: SGD
  3. response to DNA damage checkpoint signaling Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30555-MONOMER.
ReactomeiREACT_191574. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA 3'-end-processing protein RNA15
Gene namesi
Name:RNA15
Ordered Locus Names:YGL044C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL044c.
SGDiS000003012. RNA15.

Subcellular locationi

GO - Cellular componenti

  1. mRNA cleavage factor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296mRNA 3'-end-processing protein RNA15PRO_0000081807Add
BLAST

Proteomic databases

MaxQBiP25299.
PaxDbiP25299.
PeptideAtlasiP25299.

Expressioni

Gene expression databases

GenevestigatoriP25299.

Interactioni

Subunit structurei

Component of the CFIA complex, which is composed of RNA14, RNA15, PCF11 and CLP1. Interacts directly with RNA14. Interacts with polyadenylate-binding protein PAB1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PCF11P390819EBI-15640,EBI-12980
RNA14P252988EBI-15640,EBI-15632
SUB1P540002EBI-15640,EBI-18492

Protein-protein interaction databases

BioGridi33204. 116 interactions.
DIPiDIP-1489N.
IntActiP25299. 11 interactions.
MINTiMINT-389896.
STRINGi4932.YGL044C.

Structurei

Secondary structure

1
296
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 247Combined sources
Helixi31 – 399Combined sources
Beta strandi44 – 485Combined sources
Turni53 – 553Combined sources
Beta strandi60 – 689Combined sources
Helixi69 – 7911Combined sources
Beta strandi85 – 873Combined sources
Beta strandi90 – 934Combined sources
Helixi99 – 1013Combined sources
Helixi139 – 1435Combined sources
Turni156 – 1583Combined sources
Helixi161 – 17010Combined sources
Helixi174 – 19017Combined sources
Helixi192 – 20110Combined sources
Helixi203 – 21614Combined sources
Beta strandi217 – 2193Combined sources
Helixi221 – 2244Combined sources
Turni225 – 2273Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KM8NMR-B14-97[»]
2L9BNMR-A127-232[»]
2X1AX-ray2.05A16-111[»]
2X1BX-ray1.80A16-111[»]
2X1FX-ray1.60A16-103[»]
ProteinModelPortaliP25299.
SMRiP25299. Positions 16-101, 138-228.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25299.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 9679RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi104 – 1096Poly-Gln
Compositional biasi111 – 12818Asn-richAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG246793.
GeneTreeiENSGT00760000119351.
HOGENOMiHOG000247912.
KOiK14407.
OMAiQILDLCS.
OrthoDBiEOG706125.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25299-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRQSGVNAG VQNNPPSRVV YLGSIPYDQT EEQILDLCSN VGPVINLKMM
60 70 80 90 100
FDPQTGRSKG YAFIEFRDLE SSASAVRNLN GYQLGSRFLK CGYSSNSDIS
110 120 130 140 150
GVSQQQQQQY NNINGNNNNN GNNNNNSNGP DFQNSGNANF LSQKFPELPS
160 170 180 190 200
GIDVNINMTT PAMMISSELA KKPKEVQLKF LQKFQEWTRA HPEDAVSLLE
210 220 230 240 250
LCPQLSFVTA ELLLTNGICK VDDLIPLASR PQEEASATNN NSVNEVVDPA
260 270 280 290
VLNKQKELLK QVLQLNDSQI SILPDDERMA IWDLKQKALR GEFGAF
Length:296
Mass (Da):32,791
Last modified:May 1, 1992 - v1
Checksum:i10053B8648A71ECB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73462 Genomic DNA. Translation: AAA34984.1.
Z72566 Genomic DNA. Translation: CAA96746.1.
BK006941 Genomic DNA. Translation: DAA08056.1.
PIRiB40257.
RefSeqiNP_011471.1. NM_001180909.1.

Genome annotation databases

EnsemblFungiiYGL044C; YGL044C; YGL044C.
GeneIDi852838.
KEGGisce:YGL044C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73462 Genomic DNA. Translation: AAA34984.1 .
Z72566 Genomic DNA. Translation: CAA96746.1 .
BK006941 Genomic DNA. Translation: DAA08056.1 .
PIRi B40257.
RefSeqi NP_011471.1. NM_001180909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KM8 NMR - B 14-97 [» ]
2L9B NMR - A 127-232 [» ]
2X1A X-ray 2.05 A 16-111 [» ]
2X1B X-ray 1.80 A 16-111 [» ]
2X1F X-ray 1.60 A 16-103 [» ]
ProteinModelPortali P25299.
SMRi P25299. Positions 16-101, 138-228.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33204. 116 interactions.
DIPi DIP-1489N.
IntActi P25299. 11 interactions.
MINTi MINT-389896.
STRINGi 4932.YGL044C.

Proteomic databases

MaxQBi P25299.
PaxDbi P25299.
PeptideAtlasi P25299.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL044C ; YGL044C ; YGL044C .
GeneIDi 852838.
KEGGi sce:YGL044C.

Organism-specific databases

CYGDi YGL044c.
SGDi S000003012. RNA15.

Phylogenomic databases

eggNOGi NOG246793.
GeneTreei ENSGT00760000119351.
HOGENOMi HOG000247912.
KOi K14407.
OMAi QILDLCS.
OrthoDBi EOG706125.

Enzyme and pathway databases

BioCyci YEAST:G3O-30555-MONOMER.
Reactomei REACT_191574. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

EvolutionaryTracei P25299.
NextBioi 972414.

Gene expression databases

Genevestigatori P25299.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein."
    Minvielle-Sebastia L., Winsor B., Bonneaud N., Lacroute F.
    Mol. Cell. Biol. 11:3075-3087(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "RNA14 and RNA15 proteins as components of a yeast pre-mRNA 3'-end processing factor."
    Minvielle-Sebastia L., Preker P.J., Keller W.
    Science 266:1702-1705(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro."
    Amrani N., Minet M., Le Gouar M., Lacroute F., Wyers F.
    Mol. Cell. Biol. 17:3694-3701(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PAB1.
  7. "Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I."
    Gross S., Moore C.
    Proc. Natl. Acad. Sci. U.S.A. 98:6080-6085(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE CFIA COMPLEX.
  8. "Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation."
    Gross S., Moore C.L.
    Mol. Cell. Biol. 21:8045-8055(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING.
  9. "Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends."
    Kessler M.M., Zhao J., Moore C.L.
    J. Biol. Chem. 271:27167-27175(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE CFIA COMPLEX.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRNA15_YEAST
AccessioniPrimary (citable) accession number: P25299
Secondary accession number(s): D6VU95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 26, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6350 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3