mRNA 3'-end-processing protein RNA14 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P25298 (RNA14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
mRNA 3'-end-processing protein RNA14

Gene names

Name:	RNA14
Ordered Locus Names:	YMR061W
ORF Names:	YM9796.14

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Ref.5 Ref.9
Subunit structure	Component of the CFIA complex, which is composed of RNA14, RNA15, PCF11 and CLP1. Interacts with FIP1, PFS2, YSH1 and probably also with RNA15. Probably interacts with the phosphorylated CTD domain of RPB1/RNA polymerase II. Ref.7 Ref.8 Ref.10
Subcellular location	Nucleus. Cytoplasm. Note: Nucleus and/or cytoplasm.
Miscellaneous	Present with 5350 molecules/cell in log phase SD medium.
Sequence similarities	Contains 6 HAT repeats.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA cleavage Inferred from direct assay Ref.9. Source: SGD mRNA polyadenylation Inferred from direct assay Ref.9. Source: SGD
Cellular_component	mRNA cleavage factor complex Inferred from physical interaction Ref.9. Source: SGD mitochondrion Inferred from direct assay PubMed 10660071. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PCF11	P39081	12	EBI-15632,EBI-12980
RNA15	P25299	8	EBI-15632,EBI-15640

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 677

677

mRNA 3'-end-processing protein RNA14

PRO_0000205761

Regions

Repeat

56 – 88

HAT 1

Repeat

90 – 124

HAT 2

Repeat

138 – 170

HAT 3

Repeat

181 – 214

HAT 4

Repeat

257 – 289

HAT 5

Repeat

298 – 330

HAT 6

Secondary structure

677

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P25298 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 102433295FE7CD63
Show »

FASTA

677

79,960

        10         20         30         40         50         60 
MSSSTTPDLL YPSADKVAEP SDNIHGDELR LRERIKDNPT NILSYFQLIQ YLETQESYAK 

        70         80         90        100        110        120 
VREVYEQFHN TFPFYSPAWT LQLKGELARD EFETVEKILA QCLSGKLENN DLSLWSTYLD 

       130        140        150        160        170        180 
YIRRKNNLIT GGQEARAVIV KAFQLVMQKC AIFEPKSSSF WNEYLNFLEQ WKPFNKWEEQ 

       190        200        210        220        230        240 
QRIDMLREFY KKMLCVPFDN LEKMWNRYTQ WEQEINSLTA RKFIGELSAE YMKARSLYQE 

       250        260        270        280        290        300 
WLNVTNGLKR ASPINLRTAN KKNIPQPGTS DSNIQQLQIW LNWIKWEREN KLMLSEDMLS 

       310        320        330        340        350        360 
QRISYVYKQG IQYMIFSAEM WYDYSMYISE NSDRQNILYT ALLANPDSPS LTFKLSECYE 

       370        380        390        400        410        420 
LDNDSESVSN CFDKCTQTLL SQYKKIASDV NSGEDNNTEY EQELLYKQRE KLTFVFCVYM 

       430        440        450        460        470        480 
NTMKRISGLS AARTVFGKCR KLKRILTHDV YVENAYLEFQ NQNDYKTAFK VLELGLKYFQ 

       490        500        510        520        530        540 
NDGVYINKYL DFLIFLNKDS QIKTLFETSV EKVQDLTQLK EIYKKMISYE SKFGNLNNVY 

       550        560        570        580        590        600 
SLEKRFFERF PQENLIEVFT SRYQIQNSNL IKKLELTYMY NEEEDSYFSS GNGDGHHGSY 

       610        620        630        640        650        660 
NMSSSDRKRL MEETGNNGNF SNKKFKRDSE LPTEVLDLLS VIPKRQYFNT NLLDAQKLVN 

       670 
FLNDQVEIPT VESTKSG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein." Minvielle-Sebastia L., Winsor B., Bonneaud N., Lacroute F. Mol. Cell. Biol. 11:3075-3087(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	Bonneaud N. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"RNA14 and RNA15 proteins as components of a yeast pre-mRNA 3'-end processing factor." Minvielle-Sebastia L., Preker P.J., Keller W. Science 266:1702-1705(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends." Kessler M.M., Zhao J., Moore C.L. J. Biol. Chem. 271:27167-27175(1996) [PubMed] [Europe PMC] [Abstract] Cited for: COMPOSITION OF THE CFIA COMPLEX.
[7]	"The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation." Minvielle-Sebastia L., Preker P.J., Wiederkehr T., Strahm Y., Keller W. Proc. Natl. Acad. Sci. U.S.A. 94:7897-7902(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CFIA COMPLEX.
[8]	"The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex." Ohnacker M., Barabino S.M.L., Preker P.J., Keller W. EMBO J. 19:37-47(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FIP1; PFS2 AND YSH1.
[9]	"Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I." Gross S., Moore C. Proc. Natl. Acad. Sci. U.S.A. 98:6080-6085(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE CFIA COMPLEX.
[10]	"Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination." Sadowski M., Dichtl B., Huebner W., Keller W. EMBO J. 22:2167-2177(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RBP1.
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M73461 Genomic DNA. Translation: AAA21300.1.
Z49703 Genomic DNA. Translation: CAA89771.1.
BK006946 Genomic DNA. Translation: DAA09959.1.

PIR

S54561.

RefSeq

NP_013777.1. NM_001182559.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L9B	NMR	-	B	626-677	[»]

ProteinModelPortal

P25298.

SMR

P25298. Positions 30-669.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1488N.

IntAct

P25298. 40 interactions.

MINT

MINT-400599.

STRING

4932.YMR061W.

Proteomic databases

PaxDb

P25298.

PRIDE

P25298.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YMR061W; YMR061W; YMR061W.

GeneID

855083.

KEGG

sce:YMR061W.

Organism-specific databases

CYGD

YMR061w.

SGD

S000004665. RNA14.

Phylogenomic databases

eggNOG

COG5107.

GeneTree

ENSGT00390000006758.

HOGENOM

HOG000000753.

K14408.

OMA

YTKWENE.

OrthoDB

EOG49S9G9.

Gene expression databases

Genevestigator

P25298.

GermOnline

YMR061W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.25.40.10. 3 hits.

InterPro

IPR003107. HAT.
IPR008847. Suf.
IPR011990. TPR-like_helical.
[Graphical view]

Pfam

PF05843. Suf. 1 hit.
[Graphical view]

SMART

SM00386. HAT. 8 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

978372.

Entry information

Entry name

RNA14_YEAST

Accession

Primary (citable) accession number: P25298
Secondary accession number(s): D6VZN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents