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P25298

- RNA14_YEAST

UniProt

P25298 - RNA14_YEAST

Protein

mRNA 3'-end-processing protein RNA14

Gene

RNA14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein heterodimerization activity Source: SGD

    GO - Biological processi

    1. mRNA cleavage Source: SGD
    2. mRNA polyadenylation Source: SGD

    Keywords - Biological processi

    mRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32764-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    mRNA 3'-end-processing protein RNA14
    Gene namesi
    Name:RNA14
    Ordered Locus Names:YMR061W
    ORF Names:YM9796.14
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR061w.
    SGDiS000004665. RNA14.

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: Nucleus and/or cytoplasm.

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. mitochondrion Source: SGD
    3. mRNA cleavage factor complex Source: SGD
    4. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 677677mRNA 3'-end-processing protein RNA14PRO_0000205761Add
    BLAST

    Proteomic databases

    MaxQBiP25298.
    PaxDbiP25298.
    PRIDEiP25298.

    Expressioni

    Gene expression databases

    GenevestigatoriP25298.

    Interactioni

    Subunit structurei

    Component of the CFIA complex, which is composed of RNA14, RNA15, PCF11 and CLP1. Interacts with FIP1, PFS2, YSH1 and probably also with RNA15. Probably interacts with the phosphorylated CTD domain of RPB1/RNA polymerase II.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PCF11P3908112EBI-15632,EBI-12980
    RNA15P252998EBI-15632,EBI-15640

    Protein-protein interaction databases

    BioGridi35236. 99 interactions.
    DIPiDIP-1488N.
    IntActiP25298. 41 interactions.
    MINTiMINT-400599.
    STRINGi4932.YMR061W.

    Structurei

    Secondary structure

    1
    677
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi633 – 6419
    Helixi645 – 6484
    Helixi655 – 66410

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2L9BNMR-B626-677[»]
    ProteinModelPortaliP25298.
    SMRiP25298. Positions 30-669.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati56 – 8833HAT 1Add
    BLAST
    Repeati90 – 12435HAT 2Add
    BLAST
    Repeati138 – 17033HAT 3Add
    BLAST
    Repeati181 – 21434HAT 4Add
    BLAST
    Repeati257 – 28933HAT 5Add
    BLAST
    Repeati298 – 33033HAT 6Add
    BLAST

    Sequence similaritiesi

    Contains 6 HAT repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5107.
    GeneTreeiENSGT00390000006758.
    HOGENOMiHOG000000753.
    KOiK14408.
    OMAiNNDLSLW.
    OrthoDBiEOG79CZ7K.

    Family and domain databases

    Gene3Di1.25.40.10. 3 hits.
    InterProiIPR003107. HAT.
    IPR008847. Suf.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PfamiPF05843. Suf. 1 hit.
    [Graphical view]
    SMARTiSM00386. HAT. 8 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25298-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSTTPDLL YPSADKVAEP SDNIHGDELR LRERIKDNPT NILSYFQLIQ    50
    YLETQESYAK VREVYEQFHN TFPFYSPAWT LQLKGELARD EFETVEKILA 100
    QCLSGKLENN DLSLWSTYLD YIRRKNNLIT GGQEARAVIV KAFQLVMQKC 150
    AIFEPKSSSF WNEYLNFLEQ WKPFNKWEEQ QRIDMLREFY KKMLCVPFDN 200
    LEKMWNRYTQ WEQEINSLTA RKFIGELSAE YMKARSLYQE WLNVTNGLKR 250
    ASPINLRTAN KKNIPQPGTS DSNIQQLQIW LNWIKWEREN KLMLSEDMLS 300
    QRISYVYKQG IQYMIFSAEM WYDYSMYISE NSDRQNILYT ALLANPDSPS 350
    LTFKLSECYE LDNDSESVSN CFDKCTQTLL SQYKKIASDV NSGEDNNTEY 400
    EQELLYKQRE KLTFVFCVYM NTMKRISGLS AARTVFGKCR KLKRILTHDV 450
    YVENAYLEFQ NQNDYKTAFK VLELGLKYFQ NDGVYINKYL DFLIFLNKDS 500
    QIKTLFETSV EKVQDLTQLK EIYKKMISYE SKFGNLNNVY SLEKRFFERF 550
    PQENLIEVFT SRYQIQNSNL IKKLELTYMY NEEEDSYFSS GNGDGHHGSY 600
    NMSSSDRKRL MEETGNNGNF SNKKFKRDSE LPTEVLDLLS VIPKRQYFNT 650
    NLLDAQKLVN FLNDQVEIPT VESTKSG 677
    Length:677
    Mass (Da):79,960
    Last modified:February 1, 1996 - v2
    Checksum:i102433295FE7CD63
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73461 Genomic DNA. Translation: AAA21300.1.
    Z49703 Genomic DNA. Translation: CAA89771.1.
    BK006946 Genomic DNA. Translation: DAA09959.1.
    PIRiS54561.
    RefSeqiNP_013777.1. NM_001182559.1.

    Genome annotation databases

    EnsemblFungiiYMR061W; YMR061W; YMR061W.
    GeneIDi855083.
    KEGGisce:YMR061W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73461 Genomic DNA. Translation: AAA21300.1 .
    Z49703 Genomic DNA. Translation: CAA89771.1 .
    BK006946 Genomic DNA. Translation: DAA09959.1 .
    PIRi S54561.
    RefSeqi NP_013777.1. NM_001182559.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2L9B NMR - B 626-677 [» ]
    ProteinModelPortali P25298.
    SMRi P25298. Positions 30-669.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35236. 99 interactions.
    DIPi DIP-1488N.
    IntActi P25298. 41 interactions.
    MINTi MINT-400599.
    STRINGi 4932.YMR061W.

    Proteomic databases

    MaxQBi P25298.
    PaxDbi P25298.
    PRIDEi P25298.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR061W ; YMR061W ; YMR061W .
    GeneIDi 855083.
    KEGGi sce:YMR061W.

    Organism-specific databases

    CYGDi YMR061w.
    SGDi S000004665. RNA14.

    Phylogenomic databases

    eggNOGi COG5107.
    GeneTreei ENSGT00390000006758.
    HOGENOMi HOG000000753.
    KOi K14408.
    OMAi NNDLSLW.
    OrthoDBi EOG79CZ7K.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32764-MONOMER.

    Miscellaneous databases

    NextBioi 978372.
    PROi P25298.

    Gene expression databases

    Genevestigatori P25298.

    Family and domain databases

    Gene3Di 1.25.40.10. 3 hits.
    InterProi IPR003107. HAT.
    IPR008847. Suf.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    Pfami PF05843. Suf. 1 hit.
    [Graphical view ]
    SMARTi SM00386. HAT. 8 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein."
      Minvielle-Sebastia L., Winsor B., Bonneaud N., Lacroute F.
      Mol. Cell. Biol. 11:3075-3087(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. Bonneaud N.
      Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "RNA14 and RNA15 proteins as components of a yeast pre-mRNA 3'-end processing factor."
      Minvielle-Sebastia L., Preker P.J., Keller W.
      Science 266:1702-1705(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends."
      Kessler M.M., Zhao J., Moore C.L.
      J. Biol. Chem. 271:27167-27175(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF THE CFIA COMPLEX.
    7. "The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation."
      Minvielle-Sebastia L., Preker P.J., Wiederkehr T., Strahm Y., Keller W.
      Proc. Natl. Acad. Sci. U.S.A. 94:7897-7902(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CFIA COMPLEX.
    8. "The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex."
      Ohnacker M., Barabino S.M.L., Preker P.J., Keller W.
      EMBO J. 19:37-47(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FIP1; PFS2 AND YSH1.
    9. "Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I."
      Gross S., Moore C.
      Proc. Natl. Acad. Sci. U.S.A. 98:6080-6085(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE CFIA COMPLEX.
    10. "Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination."
      Sadowski M., Dichtl B., Huebner W., Keller W.
      EMBO J. 22:2167-2177(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RBP1.
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRNA14_YEAST
    AccessioniPrimary (citable) accession number: P25298
    Secondary accession number(s): D6VZN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5350 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3