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P25298 (RNA14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
mRNA 3'-end-processing protein RNA14
Gene names
Name:RNA14
Ordered Locus Names:YMR061W
ORF Names:YM9796.14
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length677 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Ref.5 Ref.9

Subunit structure

Component of the CFIA complex, which is composed of RNA14, RNA15, PCF11 and CLP1. Interacts with FIP1, PFS2, YSH1 and probably also with RNA15. Probably interacts with the phosphorylated CTD domain of RPB1/RNA polymerase II. Ref.7 Ref.8 Ref.10

Subcellular location

Nucleus. Cytoplasm. Note: Nucleus and/or cytoplasm.

Miscellaneous

Present with 5350 molecules/cell in log phase SD medium.

Sequence similarities

Contains 6 HAT repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PCF11P3908112EBI-15632,EBI-12980
RNA15P252998EBI-15632,EBI-15640

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 677677mRNA 3'-end-processing protein RNA14
PRO_0000205761

Regions

Repeat56 – 8833HAT 1
Repeat90 – 12435HAT 2
Repeat138 – 17033HAT 3
Repeat181 – 21434HAT 4
Repeat257 – 28933HAT 5
Repeat298 – 33033HAT 6

Secondary structure

....... 677
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25298 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 102433295FE7CD63

FASTA67779,960
        10         20         30         40         50         60 
MSSSTTPDLL YPSADKVAEP SDNIHGDELR LRERIKDNPT NILSYFQLIQ YLETQESYAK 

        70         80         90        100        110        120 
VREVYEQFHN TFPFYSPAWT LQLKGELARD EFETVEKILA QCLSGKLENN DLSLWSTYLD 

       130        140        150        160        170        180 
YIRRKNNLIT GGQEARAVIV KAFQLVMQKC AIFEPKSSSF WNEYLNFLEQ WKPFNKWEEQ 

       190        200        210        220        230        240 
QRIDMLREFY KKMLCVPFDN LEKMWNRYTQ WEQEINSLTA RKFIGELSAE YMKARSLYQE 

       250        260        270        280        290        300 
WLNVTNGLKR ASPINLRTAN KKNIPQPGTS DSNIQQLQIW LNWIKWEREN KLMLSEDMLS 

       310        320        330        340        350        360 
QRISYVYKQG IQYMIFSAEM WYDYSMYISE NSDRQNILYT ALLANPDSPS LTFKLSECYE 

       370        380        390        400        410        420 
LDNDSESVSN CFDKCTQTLL SQYKKIASDV NSGEDNNTEY EQELLYKQRE KLTFVFCVYM 

       430        440        450        460        470        480 
NTMKRISGLS AARTVFGKCR KLKRILTHDV YVENAYLEFQ NQNDYKTAFK VLELGLKYFQ 

       490        500        510        520        530        540 
NDGVYINKYL DFLIFLNKDS QIKTLFETSV EKVQDLTQLK EIYKKMISYE SKFGNLNNVY 

       550        560        570        580        590        600 
SLEKRFFERF PQENLIEVFT SRYQIQNSNL IKKLELTYMY NEEEDSYFSS GNGDGHHGSY 

       610        620        630        640        650        660 
NMSSSDRKRL MEETGNNGNF SNKKFKRDSE LPTEVLDLLS VIPKRQYFNT NLLDAQKLVN 

       670 
FLNDQVEIPT VESTKSG 

« Hide

References

« Hide 'large scale' references
[1]"Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein."
Minvielle-Sebastia L., Winsor B., Bonneaud N., Lacroute F.
Mol. Cell. Biol. 11:3075-3087(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]Bonneaud N.
Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"RNA14 and RNA15 proteins as components of a yeast pre-mRNA 3'-end processing factor."
Minvielle-Sebastia L., Preker P.J., Keller W.
Science 266:1702-1705(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends."
Kessler M.M., Zhao J., Moore C.L.
J. Biol. Chem. 271:27167-27175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF THE CFIA COMPLEX.
[7]"The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation."
Minvielle-Sebastia L., Preker P.J., Wiederkehr T., Strahm Y., Keller W.
Proc. Natl. Acad. Sci. U.S.A. 94:7897-7902(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CFIA COMPLEX.
[8]"The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex."
Ohnacker M., Barabino S.M.L., Preker P.J., Keller W.
EMBO J. 19:37-47(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FIP1; PFS2 AND YSH1.
[9]"Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I."
Gross S., Moore C.
Proc. Natl. Acad. Sci. U.S.A. 98:6080-6085(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE CFIA COMPLEX.
[10]"Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination."
Sadowski M., Dichtl B., Huebner W., Keller W.
EMBO J. 22:2167-2177(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RBP1.
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M73461 Genomic DNA. Translation: AAA21300.1.
Z49703 Genomic DNA. Translation: CAA89771.1.
BK006946 Genomic DNA. Translation: DAA09959.1.
PIRS54561.
RefSeqNP_013777.1. NM_001182559.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2L9BNMR-B626-677[»]
ProteinModelPortalP25298.
SMRP25298. Positions 30-669.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35236. 99 interactions.
DIPDIP-1488N.
IntActP25298. 41 interactions.
MINTMINT-400599.
STRING4932.YMR061W.

Proteomic databases

MaxQBP25298.
PaxDbP25298.
PRIDEP25298.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR061W; YMR061W; YMR061W.
GeneID855083.
KEGGsce:YMR061W.

Organism-specific databases

CYGDYMR061w.
SGDS000004665. RNA14.

Phylogenomic databases

eggNOGCOG5107.
GeneTreeENSGT00390000006758.
HOGENOMHOG000000753.
KOK14408.
OMANNDLSLW.
OrthoDBEOG79CZ7K.

Enzyme and pathway databases

BioCycYEAST:G3O-32764-MONOMER.

Gene expression databases

GenevestigatorP25298.

Family and domain databases

Gene3D1.25.40.10. 3 hits.
InterProIPR003107. HAT.
IPR008847. Suf.
IPR011990. TPR-like_helical.
[Graphical view]
PfamPF05843. Suf. 1 hit.
[Graphical view]
SMARTSM00386. HAT. 8 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio978372.
PROP25298.

Entry information

Entry nameRNA14_YEAST
AccessionPrimary (citable) accession number: P25298
Secondary accession number(s): D6VZN5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references