##gff-version 3 P25297 UniProtKB Chain 1 587 . . . ID=PRO_0000050477;Note=Inorganic phosphate transporter PHO84 P25297 UniProtKB Topological domain 1 67 . . . Note=Extracellular;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10518922;Dbxref=PMID:10518922 P25297 UniProtKB Transmembrane 68 88 . . . Note=Helical%3B Name%3D1;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 89 108 . . . Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 109 129 . . . Note=Helical%3B Name%3D2;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 130 133 . . . Note=Extracellular;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 134 154 . . . Note=Helical%3B Name%3D3;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 155 156 . . . Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 157 177 . . . Note=Helical%3B Name%3D4;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 178 201 . . . Note=Extracellular;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 202 222 . . . Note=Helical%3B Name%3D5;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 223 250 . . . Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 251 271 . . . Note=Helical%3B Name%3D6;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 272 345 . . . Note=Extracellular;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 346 366 . . . Note=Helical%3B Name%3D7;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 367 395 . . . Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 396 416 . . . Note=Helical%3B Name%3D8;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 417 419 . . . Note=Extracellular;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 420 440 . . . Note=Helical%3B Name%3D9;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 441 442 . . . Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 443 463 . . . Note=Helical%3B Name%3D10;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 464 485 . . . Note=Extracellular;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 486 506 . . . Note=Helical%3B Name%3D11;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 507 522 . . . Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Transmembrane 523 543 . . . Note=Helical%3B Name%3D12;Ontology_term=ECO:0000255;evidence=ECO:0000255 P25297 UniProtKB Topological domain 544 587 . . . Note=Extracellular;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10518922;Dbxref=PMID:10518922 P25297 UniProtKB Region 568 587 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P25297 UniProtKB Modified residue 302 302 . . . Note=Phosphothreonine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17330950,ECO:0007744|PubMed:19779198;Dbxref=PMID:17330950,PMID:19779198 P25297 UniProtKB Modified residue 303 303 . . . Note=Phosphoserine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17330950,ECO:0007744|PubMed:19779198;Dbxref=PMID:17330950,PMID:19779198 P25297 UniProtKB Modified residue 316 316 . . . Note=Phosphoserine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17330950,ECO:0007744|PubMed:19779198;Dbxref=PMID:17330950,PMID:19779198 P25297 UniProtKB Modified residue 317 317 . . . Note=Phosphothreonine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17330950,ECO:0007744|PubMed:19779198;Dbxref=PMID:17330950,PMID:19779198 P25297 UniProtKB Modified residue 321 321 . . . Note=Phosphoserine;Ontology_term=ECO:0007744,ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17330950,ECO:0007744|PubMed:18407956,ECO:0007744|PubMed:19779198;Dbxref=PMID:17330950,PMID:18407956,PMID:19779198 P25297 UniProtKB Modified residue 577 577 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:17330950;Dbxref=PMID:17330950 P25297 UniProtKB Modified residue 579 579 . . . Note=Phosphoserine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17330950,ECO:0007744|PubMed:19779198;Dbxref=PMID:17330950,PMID:19779198 P25297 UniProtKB Modified residue 581 581 . . . Note=Phosphoserine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17330950,ECO:0007744|PubMed:19779198;Dbxref=PMID:17330950,PMID:19779198 P25297 UniProtKB Cross-link 6 6 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin);Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12872131;Dbxref=PMID:12872131 P25297 UniProtKB Cross-link 298 298 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin);Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12872131;Dbxref=PMID:12872131 P25297 UniProtKB Mutagenesis 145 145 . . . Note=No significant effect on transport activity%3B when associated with S-237%2C S-241%2C S-245%2C S-263%2C S-335%2C S-399%2C S-434%2C S-455%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 160 160 . . . Note=Reduces phosphate binding%2C transport and signaling activities. F->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20133652;Dbxref=PMID:20133652 P25297 UniProtKB Mutagenesis 168 168 . . . Note=Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 168 168 . . . Note=Abolishes transport and signaling activities. R->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20133652;Dbxref=PMID:20133652 P25297 UniProtKB Mutagenesis 168 168 . . . Note=Reduces transport activity. Moderately reduces affinity for inorganic phosphate. No significant effects on growth rates. R->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 168 168 . . . Note=Reduces transport activity. Moderately reduces affinity for inorganic phosphate. Moderately reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions. R->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 172 172 . . . Note=Abolishes transport and signaling activities. G->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20133652;Dbxref=PMID:20133652 P25297 UniProtKB Mutagenesis 174 174 . . . Note=Abolishes transport and signaling activities. G->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20133652;Dbxref=PMID:20133652 P25297 UniProtKB Mutagenesis 176 176 . . . Note=Abolishes transport and signaling activities. G->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20133652;Dbxref=PMID:20133652 P25297 UniProtKB Mutagenesis 178 178 . . . Note=Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on signaling activities. No significant effects on growth rates. D->E%2CN;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 179 179 . . . Note=Significantly reduces transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Slightly reduces signaling activity. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27875295;Dbxref=PMID:27875295 P25297 UniProtKB Mutagenesis 179 179 . . . Note=Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Abolishes signaling activity. Y->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27875295;Dbxref=PMID:27875295 P25297 UniProtKB Mutagenesis 179 179 . . . Note=No significant effects on transport activity. No significant effects on growth rates under low phosphate conditions. Does not affect patterns of secreted phosphatase activities under low and high phosphate conditions. No significant effects on signaling activity. Y->S%2CF;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27875295;Dbxref=PMID:27875295 P25297 UniProtKB Mutagenesis 237 237 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-241%2C S-245%2C S-263%2C S-335%2C S-399%2C S-434%2C S-455%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 241 241 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-245%2C S-263%2C S-335%2C S-399%2C S-434%2C S-455%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 245 245 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-263%2C S-335%2C S-399%2C S-434%2C S-455%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 263 263 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-245%2C S-335%2C S-399%2C S-434%2C S-455%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 298 298 . . . Note=No significant effect on membrane localization under low phosphate conditions and internalization after phosphate addition. Strongly affects vacuolar sorting of the protein after internalization. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19348508;Dbxref=PMID:19348508 P25297 UniProtKB Mutagenesis 304 327 . . . Note=Increases transport activity. Has no significant effect on membrane localization under low phosphate conditions. Results in severely delayed internalization after phosphate addition. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19348508;Dbxref=PMID:19348508 P25297 UniProtKB Mutagenesis 335 335 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-245%2C S-263%2C S-399%2C S-434%2C S-455%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 358 358 . . . Note=Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Moderately decreases signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions. D->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 358 358 . . . Note=Significantly reduces transport activity. No significant effects on affinity for inorganic phosphate. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. No significant effects on signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 392 392 . . . Note=Reduces transport and signaling activities. V->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20133652;Dbxref=PMID:20133652 P25297 UniProtKB Mutagenesis 399 399 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-245%2C S-263%2C S-335%2C S-434%2C S-455%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 408 408 . . . Note=Reduces signaling activity and enhances transport activity. V->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20133652;Dbxref=PMID:20133652 P25297 UniProtKB Mutagenesis 434 434 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-245%2C S-263%2C S-335%2C S-399%2C S-455%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 455 455 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-245%2C S-263%2C S-335%2C S-399%2C S-434%2C S-474%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 473 473 . . . Note=No significant effects on transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates. E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 473 473 . . . Note=No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates. E->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 474 474 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-245%2C S-263%2C S-335%2C S-399%2C S-434%2C S-455%2C S-510 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 492 492 . . . Note=No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates. K->A%2CQ;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 492 492 . . . Note=Reduces transport activity. Reduces affinity for inorganic phosphate. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions. K->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22587366;Dbxref=PMID:22587366 P25297 UniProtKB Mutagenesis 510 510 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-245%2C S-263%2C S-335%2C S-399%2C S-434%2C S-455%2C S-474 and S-519. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Mutagenesis 519 519 . . . Note=No significant effect on transport activity%3B when associated with S-145%2C S-237%2C S-241%2C S-245%2C S-263%2C S-335%2C S-399%2C S-434%2C S-455%2C S-474 and S-510. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11573939;Dbxref=PMID:11573939 P25297 UniProtKB Sequence conflict 162 162 . . . Note=A->AHSPAINFVA;Ontology_term=ECO:0000305;evidence=ECO:0000305 P25297 UniProtKB Sequence conflict 353 353 . . . Note=S->Y;Ontology_term=ECO:0000305;evidence=ECO:0000305