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P25297 (PHO84_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inorganic phosphate transporter PHO84
Gene names
Name:PHO84
Ordered Locus Names:YML123C
ORF Names:YM7056.03C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity transporter for external inorganic phosphate. Is not an essential protein, since a constitutive, low affinity pI transporter exists in yeast.

Subunit structure

May function as a monomer.

Subcellular location

Membrane; Multi-pass membrane protein.

Induction

Under the control of phosphate. It is derepressed by phosphate starvation.

Miscellaneous

Present with 335000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 587587Inorganic phosphate transporter PHO84
PRO_0000050477

Regions

Topological domain1 – 6767Cytoplasmic Potential
Transmembrane68 – 8821Helical; Name=1; Potential
Topological domain89 – 10820Extracellular Potential
Transmembrane109 – 12921Helical; Name=2; Potential
Topological domain130 – 1334Cytoplasmic Potential
Transmembrane134 – 15421Helical; Name=3; Potential
Topological domain155 – 1562Extracellular Potential
Transmembrane157 – 17721Helical; Name=4; Potential
Topological domain178 – 20124Cytoplasmic Potential
Transmembrane202 – 22221Helical; Name=5; Potential
Topological domain223 – 25028Extracellular Potential
Transmembrane251 – 27121Helical; Name=6; Potential
Topological domain272 – 34574Cytoplasmic Potential
Transmembrane346 – 36621Helical; Name=7; Potential
Topological domain367 – 39529Extracellular Potential
Transmembrane396 – 41621Helical; Name=8; Potential
Topological domain417 – 4193Cytoplasmic Potential
Transmembrane420 – 44021Helical; Name=9; Potential
Topological domain441 – 4422Extracellular Potential
Transmembrane443 – 46321Helical; Name=10; Potential
Topological domain464 – 48522Cytoplasmic Potential
Transmembrane486 – 50621Helical; Name=11; Potential
Topological domain507 – 52216Extracellular Potential
Transmembrane523 – 54321Helical; Name=12; Potential
Topological domain544 – 58744Cytoplasmic Potential

Amino acid modifications

Modified residue3021Phosphothreonine Ref.6 Ref.8
Modified residue3031Phosphoserine Ref.6 Ref.8
Modified residue3161Phosphoserine Ref.6 Ref.8
Modified residue3171Phosphothreonine Ref.6 Ref.8
Modified residue3211Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue5771Phosphoserine Ref.6
Modified residue5791Phosphoserine Ref.6 Ref.8
Modified residue5811Phosphoserine Ref.6 Ref.8
Cross-link6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5
Cross-link298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Experimental info

Sequence conflict1621A → AHSPAINFVA Ref.1
Sequence conflict3531S → Y in BAA14358. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25297 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E463954395E8A840

FASTA58764,382
        10         20         30         40         50         60 
MSSVNKDTIH VAERSLHKEH LTEGGNMAFH NHLNDFAHIE DPLERRRLAL ESIDDEGFGW 

        70         80         90        100        110        120 
QQVKTISIAG VGFLTDSYDI FAINLGITMM SYVYWHGSMP GPSQTLLKVS TSVGTVIGQF 

       130        140        150        160        170        180 
GFGTLADIVG RKRIYGMELI IMIVCTILQT TVAHSPAINF VAVLTFYRIV MGIGIGGDYP 

       190        200        210        220        230        240 
LSSIITSEFA TTKWRGAIMG AVFANQAWGQ ISGGIIALIL VAAYKGELEY ANSGAECDAR 

       250        260        270        280        290        300 
CQKACDQMWR ILIGLGTVLG LACLYFRLTI PESPRYQLDV NAKLELAAAA QEQDGEKKIH 

       310        320        330        340        350        360 
DTSDEDMAIN GLERASTAVE SLDNHPPKAS FKDFCRHFGQ WKYGKILLGT AGSWFTLDVA 

       370        380        390        400        410        420 
FYGLSLNSAV ILQTIGYAGS KNVYKKLYDT AVGNLILICA GSLPGYWVSV FTVDIIGRKP 

       430        440        450        460        470        480 
IQLAGFIILT ALFCVIGFAY HKLGDHGLLA LYVICQFFQN FGPNTTTFIV PGECFPTRYR 

       490        500        510        520        530        540 
STAHGISAAS GKVGAIIAQT ALGTLIDHNC ARDGKPTNCW LPHVMEIFAL FMLLGIFTTL 

       550        560        570        580 
LIPETKRKTL EEINELYHDE IDPATLNFRN KNNDIESSSP SQLQHEA 

« Hide

References

« Hide 'large scale' references
[1]"The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter."
Bun-Ya M., Nishimura M., Harashima S., Oshima Y.
Mol. Cell. Biol. 11:3229-3238(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-6 AND LYS-298.
Strain: SUB592.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302; SER-303; SER-316; THR-317; SER-321; SER-577; SER-579 AND SER-581, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302; SER-303; SER-316; THR-317; SER-321; SER-579 AND SER-581, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90346 Genomic DNA. Translation: BAA14358.1.
Z49218 Genomic DNA. Translation: CAA89157.1.
BK006946 Genomic DNA. Translation: DAA09776.1.
PIRS54061.
RefSeqNP_013583.1. NM_001182486.1.

3D structure databases

ProteinModelPortalP25297.
SMRP25297. Positions 62-557.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35082. 135 interactions.
DIPDIP-5072N.
IntActP25297. 1 interaction.
MINTMINT-498423.
STRING4932.YML123C.

Protein family/group databases

TCDB2.A.1.9.1. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbP25297.
PeptideAtlasP25297.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML123C; YML123C; YML123C.
GeneID854916.
KEGGsce:YML123C.

Organism-specific databases

CYGDYML123c.
SGDS000004592. PHO84.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00750000118743.
HOGENOMHOG000171119.
KOK08176.
OMACQFFQNF.
OrthoDBEOG7RNK80.

Enzyme and pathway databases

BioCycYEAST:G3O-32702-MONOMER.

Gene expression databases

GenevestigatorP25297.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004738. Phos_permease.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 2 hits.
TIGRFAMsTIGR00887. 2A0109. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977923.
PROP25297.

Entry information

Entry namePHO84_YEAST
AccessionPrimary (citable) accession number: P25297
Secondary accession number(s): D6W0G2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families