Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inorganic phosphate transporter PHO84

Gene

PHO84

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for external inorganic phosphate. Is not an essential protein, since a constitutive, low affinity pI transporter exists in yeast.

GO - Molecular functioni

  • inorganic phosphate transmembrane transporter activity Source: SGD
  • manganese ion transmembrane transporter activity Source: SGD
  • selenite:proton symporter activity Source: SGD

GO - Biological processi

  • manganese ion transport Source: SGD
  • phosphate ion transport Source: SGD
  • plasma membrane selenite transport Source: SGD
  • polyphosphate metabolic process Source: SGD
  • positive regulation of replicative senescence Source: SGD
Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32702-MONOMER.
BRENDAi3.6.3.27. 984.

Protein family/group databases

TCDBi2.A.1.9.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic phosphate transporter PHO84
Gene namesi
Name:PHO84
Ordered Locus Names:YML123C
ORF Names:YM7056.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML123C.
SGDiS000004592. PHO84.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 67CytoplasmicSequence analysisAdd BLAST67
Transmembranei68 – 88Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini89 – 108ExtracellularSequence analysisAdd BLAST20
Transmembranei109 – 129Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini130 – 133CytoplasmicSequence analysis4
Transmembranei134 – 154Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini155 – 156ExtracellularSequence analysis2
Transmembranei157 – 177Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini178 – 201CytoplasmicSequence analysisAdd BLAST24
Transmembranei202 – 222Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini223 – 250ExtracellularSequence analysisAdd BLAST28
Transmembranei251 – 271Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini272 – 345CytoplasmicSequence analysisAdd BLAST74
Transmembranei346 – 366Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini367 – 395ExtracellularSequence analysisAdd BLAST29
Transmembranei396 – 416Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini417 – 419CytoplasmicSequence analysis3
Transmembranei420 – 440Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini441 – 442ExtracellularSequence analysis2
Transmembranei443 – 463Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini464 – 485CytoplasmicSequence analysisAdd BLAST22
Transmembranei486 – 506Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini507 – 522ExtracellularSequence analysisAdd BLAST16
Transmembranei523 – 543Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini544 – 587CytoplasmicSequence analysisAdd BLAST44

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000504771 – 587Inorganic phosphate transporter PHO84Add BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei302PhosphothreonineCombined sources1
Modified residuei303PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei317PhosphothreonineCombined sources1
Modified residuei321PhosphoserineCombined sources1
Modified residuei577PhosphoserineCombined sources1
Modified residuei579PhosphoserineCombined sources1
Modified residuei581PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP25297.
PRIDEiP25297.
TopDownProteomicsiP25297.

PTM databases

iPTMnetiP25297.

Expressioni

Inductioni

Under the control of phosphate. It is derepressed by phosphate starvation.

Interactioni

Subunit structurei

May function as a monomer.

Protein-protein interaction databases

BioGridi35082. 138 interactors.
DIPiDIP-5072N.
IntActiP25297. 2 interactors.
MINTiMINT-498423.

Structurei

3D structure databases

ProteinModelPortaliP25297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000171119.
InParanoidiP25297.
KOiK08176.
OMAiLNDFAHI.
OrthoDBiEOG092C2CQ7.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004738. Phos_permease.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00887. 2A0109. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVNKDTIH VAERSLHKEH LTEGGNMAFH NHLNDFAHIE DPLERRRLAL
60 70 80 90 100
ESIDDEGFGW QQVKTISIAG VGFLTDSYDI FAINLGITMM SYVYWHGSMP
110 120 130 140 150
GPSQTLLKVS TSVGTVIGQF GFGTLADIVG RKRIYGMELI IMIVCTILQT
160 170 180 190 200
TVAHSPAINF VAVLTFYRIV MGIGIGGDYP LSSIITSEFA TTKWRGAIMG
210 220 230 240 250
AVFANQAWGQ ISGGIIALIL VAAYKGELEY ANSGAECDAR CQKACDQMWR
260 270 280 290 300
ILIGLGTVLG LACLYFRLTI PESPRYQLDV NAKLELAAAA QEQDGEKKIH
310 320 330 340 350
DTSDEDMAIN GLERASTAVE SLDNHPPKAS FKDFCRHFGQ WKYGKILLGT
360 370 380 390 400
AGSWFTLDVA FYGLSLNSAV ILQTIGYAGS KNVYKKLYDT AVGNLILICA
410 420 430 440 450
GSLPGYWVSV FTVDIIGRKP IQLAGFIILT ALFCVIGFAY HKLGDHGLLA
460 470 480 490 500
LYVICQFFQN FGPNTTTFIV PGECFPTRYR STAHGISAAS GKVGAIIAQT
510 520 530 540 550
ALGTLIDHNC ARDGKPTNCW LPHVMEIFAL FMLLGIFTTL LIPETKRKTL
560 570 580
EEINELYHDE IDPATLNFRN KNNDIESSSP SQLQHEA
Length:587
Mass (Da):64,382
Last modified:February 1, 1996 - v2
Checksum:iE463954395E8A840
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162A → AHSPAINFVA (PubMed:2038328).Curated1
Sequence conflicti353S → Y in BAA14358 (PubMed:2038328).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90346 Genomic DNA. Translation: BAA14358.1.
Z49218 Genomic DNA. Translation: CAA89157.1.
BK006946 Genomic DNA. Translation: DAA09776.1.
PIRiS54061.
RefSeqiNP_013583.1. NM_001182486.1.

Genome annotation databases

EnsemblFungiiYML123C; YML123C; YML123C.
GeneIDi854916.
KEGGisce:YML123C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90346 Genomic DNA. Translation: BAA14358.1.
Z49218 Genomic DNA. Translation: CAA89157.1.
BK006946 Genomic DNA. Translation: DAA09776.1.
PIRiS54061.
RefSeqiNP_013583.1. NM_001182486.1.

3D structure databases

ProteinModelPortaliP25297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35082. 138 interactors.
DIPiDIP-5072N.
IntActiP25297. 2 interactors.
MINTiMINT-498423.

Protein family/group databases

TCDBi2.A.1.9.1. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP25297.

Proteomic databases

MaxQBiP25297.
PRIDEiP25297.
TopDownProteomicsiP25297.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML123C; YML123C; YML123C.
GeneIDi854916.
KEGGisce:YML123C.

Organism-specific databases

EuPathDBiFungiDB:YML123C.
SGDiS000004592. PHO84.

Phylogenomic databases

HOGENOMiHOG000171119.
InParanoidiP25297.
KOiK08176.
OMAiLNDFAHI.
OrthoDBiEOG092C2CQ7.

Enzyme and pathway databases

BioCyciYEAST:G3O-32702-MONOMER.
BRENDAi3.6.3.27. 984.

Miscellaneous databases

PROiP25297.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004738. Phos_permease.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00887. 2A0109. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHO84_YEAST
AccessioniPrimary (citable) accession number: P25297
Secondary accession number(s): D6W0G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 335000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.