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P25297

- PHO84_YEAST

UniProt

P25297 - PHO84_YEAST

Protein

Inorganic phosphate transporter PHO84

Gene

PHO84

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    High-affinity transporter for external inorganic phosphate. Is not an essential protein, since a constitutive, low affinity pI transporter exists in yeast.

    GO - Molecular functioni

    1. inorganic phosphate transmembrane transporter activity Source: SGD
    2. manganese ion transmembrane transporter activity Source: SGD
    3. selenite:proton symporter activity Source: SGD

    GO - Biological processi

    1. manganese ion transmembrane transport Source: GOC
    2. manganese ion transport Source: SGD
    3. phosphate ion transport Source: SGD
    4. plasma membrane selenite transport Source: SGD
    5. polyphosphate metabolic process Source: SGD
    6. proton transport Source: GOC

    Keywords - Biological processi

    Phosphate transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32702-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.9.1. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inorganic phosphate transporter PHO84
    Gene namesi
    Name:PHO84
    Ordered Locus Names:YML123C
    ORF Names:YM7056.03C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML123c.
    SGDiS000004592. PHO84.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 587587Inorganic phosphate transporter PHO84PRO_0000050477Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki6 – 6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki298 – 298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Modified residuei302 – 3021Phosphothreonine2 Publications
    Modified residuei303 – 3031Phosphoserine2 Publications
    Modified residuei316 – 3161Phosphoserine2 Publications
    Modified residuei317 – 3171Phosphothreonine2 Publications
    Modified residuei321 – 3211Phosphoserine3 Publications
    Modified residuei577 – 5771Phosphoserine1 Publication
    Modified residuei579 – 5791Phosphoserine2 Publications
    Modified residuei581 – 5811Phosphoserine2 Publications

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP25297.
    PaxDbiP25297.
    PeptideAtlasiP25297.

    Expressioni

    Inductioni

    Under the control of phosphate. It is derepressed by phosphate starvation.

    Gene expression databases

    GenevestigatoriP25297.

    Interactioni

    Subunit structurei

    May function as a monomer.

    Protein-protein interaction databases

    BioGridi35082. 136 interactions.
    DIPiDIP-5072N.
    IntActiP25297. 1 interaction.
    MINTiMINT-498423.
    STRINGi4932.YML123C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25297.
    SMRiP25297. Positions 62-552.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6767CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini89 – 10820ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini130 – 1334CytoplasmicSequence Analysis
    Topological domaini155 – 1562ExtracellularSequence Analysis
    Topological domaini178 – 20124CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini223 – 25028ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini272 – 34574CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini367 – 39529ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini417 – 4193CytoplasmicSequence Analysis
    Topological domaini441 – 4422ExtracellularSequence Analysis
    Topological domaini464 – 48522CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini507 – 52216ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini544 – 58744CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei68 – 8821Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei109 – 12921Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei134 – 15421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei157 – 17721Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei202 – 22221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei251 – 27121Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei346 – 36621Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei396 – 41621Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei420 – 44021Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei443 – 46321Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei486 – 50621Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei523 – 54321Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00750000118743.
    HOGENOMiHOG000171119.
    KOiK08176.
    OMAiLYHFMAI.
    OrthoDBiEOG7RNK80.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004738. Phos_permease.
    IPR005828. Sub_transporter.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00887. 2A0109. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25297-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSVNKDTIH VAERSLHKEH LTEGGNMAFH NHLNDFAHIE DPLERRRLAL    50
    ESIDDEGFGW QQVKTISIAG VGFLTDSYDI FAINLGITMM SYVYWHGSMP 100
    GPSQTLLKVS TSVGTVIGQF GFGTLADIVG RKRIYGMELI IMIVCTILQT 150
    TVAHSPAINF VAVLTFYRIV MGIGIGGDYP LSSIITSEFA TTKWRGAIMG 200
    AVFANQAWGQ ISGGIIALIL VAAYKGELEY ANSGAECDAR CQKACDQMWR 250
    ILIGLGTVLG LACLYFRLTI PESPRYQLDV NAKLELAAAA QEQDGEKKIH 300
    DTSDEDMAIN GLERASTAVE SLDNHPPKAS FKDFCRHFGQ WKYGKILLGT 350
    AGSWFTLDVA FYGLSLNSAV ILQTIGYAGS KNVYKKLYDT AVGNLILICA 400
    GSLPGYWVSV FTVDIIGRKP IQLAGFIILT ALFCVIGFAY HKLGDHGLLA 450
    LYVICQFFQN FGPNTTTFIV PGECFPTRYR STAHGISAAS GKVGAIIAQT 500
    ALGTLIDHNC ARDGKPTNCW LPHVMEIFAL FMLLGIFTTL LIPETKRKTL 550
    EEINELYHDE IDPATLNFRN KNNDIESSSP SQLQHEA 587
    Length:587
    Mass (Da):64,382
    Last modified:February 1, 1996 - v2
    Checksum:iE463954395E8A840
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti162 – 1621A → AHSPAINFVA(PubMed:2038328)Curated
    Sequence conflicti353 – 3531S → Y in BAA14358. (PubMed:2038328)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90346 Genomic DNA. Translation: BAA14358.1.
    Z49218 Genomic DNA. Translation: CAA89157.1.
    BK006946 Genomic DNA. Translation: DAA09776.1.
    PIRiS54061.
    RefSeqiNP_013583.1. NM_001182486.1.

    Genome annotation databases

    EnsemblFungiiYML123C; YML123C; YML123C.
    GeneIDi854916.
    KEGGisce:YML123C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90346 Genomic DNA. Translation: BAA14358.1 .
    Z49218 Genomic DNA. Translation: CAA89157.1 .
    BK006946 Genomic DNA. Translation: DAA09776.1 .
    PIRi S54061.
    RefSeqi NP_013583.1. NM_001182486.1.

    3D structure databases

    ProteinModelPortali P25297.
    SMRi P25297. Positions 62-552.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35082. 136 interactions.
    DIPi DIP-5072N.
    IntActi P25297. 1 interaction.
    MINTi MINT-498423.
    STRINGi 4932.YML123C.

    Protein family/group databases

    TCDBi 2.A.1.9.1. the major facilitator superfamily (mfs).

    Proteomic databases

    MaxQBi P25297.
    PaxDbi P25297.
    PeptideAtlasi P25297.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML123C ; YML123C ; YML123C .
    GeneIDi 854916.
    KEGGi sce:YML123C.

    Organism-specific databases

    CYGDi YML123c.
    SGDi S000004592. PHO84.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00750000118743.
    HOGENOMi HOG000171119.
    KOi K08176.
    OMAi LYHFMAI.
    OrthoDBi EOG7RNK80.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32702-MONOMER.

    Miscellaneous databases

    NextBioi 977923.
    PROi P25297.

    Gene expression databases

    Genevestigatori P25297.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004738. Phos_permease.
    IPR005828. Sub_transporter.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00887. 2A0109. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter."
      Bun-Ya M., Nishimura M., Harashima S., Oshima Y.
      Mol. Cell. Biol. 11:3229-3238(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-6 AND LYS-298.
      Strain: SUB592.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302; SER-303; SER-316; THR-317; SER-321; SER-577; SER-579 AND SER-581, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302; SER-303; SER-316; THR-317; SER-321; SER-579 AND SER-581, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPHO84_YEAST
    AccessioniPrimary (citable) accession number: P25297
    Secondary accession number(s): D6W0G2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 335000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3