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Protein

Inorganic phosphate transporter PHO84

Gene

PHO84

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for external inorganic phosphate. Is not an essential protein, since a constitutive, low affinity pI transporter exists in yeast.

GO - Molecular functioni

  • inorganic phosphate transmembrane transporter activity Source: SGD
  • manganese ion transmembrane transporter activity Source: SGD
  • selenite:proton symporter activity Source: SGD

GO - Biological processi

  • manganese ion transmembrane transport Source: GOC
  • manganese ion transport Source: SGD
  • phosphate ion transport Source: SGD
  • plasma membrane selenite transport Source: SGD
  • polyphosphate metabolic process Source: SGD
  • proton transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32702-MONOMER.
BRENDAi3.6.3.27. 984.

Protein family/group databases

TCDBi2.A.1.9.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic phosphate transporter PHO84
Gene namesi
Name:PHO84
Ordered Locus Names:YML123C
ORF Names:YM7056.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYML123c.
EuPathDBiFungiDB:YML123C.
SGDiS000004592. PHO84.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6767CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei68 – 8821Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini89 – 10820ExtracellularSequence AnalysisAdd
BLAST
Transmembranei109 – 12921Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini130 – 1334CytoplasmicSequence Analysis
Transmembranei134 – 15421Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini155 – 1562ExtracellularSequence Analysis
Transmembranei157 – 17721Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini178 – 20124CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei202 – 22221Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini223 – 25028ExtracellularSequence AnalysisAdd
BLAST
Transmembranei251 – 27121Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini272 – 34574CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei346 – 36621Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini367 – 39529ExtracellularSequence AnalysisAdd
BLAST
Transmembranei396 – 41621Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini417 – 4193CytoplasmicSequence Analysis
Transmembranei420 – 44021Helical; Name=9Sequence AnalysisAdd
BLAST
Topological domaini441 – 4422ExtracellularSequence Analysis
Transmembranei443 – 46321Helical; Name=10Sequence AnalysisAdd
BLAST
Topological domaini464 – 48522CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei486 – 50621Helical; Name=11Sequence AnalysisAdd
BLAST
Topological domaini507 – 52216ExtracellularSequence AnalysisAdd
BLAST
Transmembranei523 – 54321Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini544 – 58744CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587Inorganic phosphate transporter PHO84PRO_0000050477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki6 – 6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki298 – 298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei302 – 3021Phosphothreonine2 Publications
Modified residuei303 – 3031Phosphoserine2 Publications
Modified residuei316 – 3161Phosphoserine2 Publications
Modified residuei317 – 3171Phosphothreonine2 Publications
Modified residuei321 – 3211Phosphoserine3 Publications
Modified residuei577 – 5771Phosphoserine1 Publication
Modified residuei579 – 5791Phosphoserine2 Publications
Modified residuei581 – 5811Phosphoserine2 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP25297.
PaxDbiP25297.
PeptideAtlasiP25297.

Expressioni

Inductioni

Under the control of phosphate. It is derepressed by phosphate starvation.

Interactioni

Subunit structurei

May function as a monomer.

Protein-protein interaction databases

BioGridi35082. 138 interactions.
DIPiDIP-5072N.
IntActiP25297. 2 interactions.
MINTiMINT-498423.
STRINGi4932.YML123C.

Structurei

3D structure databases

ProteinModelPortaliP25297.
SMRiP25297. Positions 62-552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00750000118743.
HOGENOMiHOG000171119.
InParanoidiP25297.
KOiK08176.
OMAiVTSAKCE.
OrthoDBiEOG7RNK80.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR004738. Phos_permease.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00887. 2A0109. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVNKDTIH VAERSLHKEH LTEGGNMAFH NHLNDFAHIE DPLERRRLAL
60 70 80 90 100
ESIDDEGFGW QQVKTISIAG VGFLTDSYDI FAINLGITMM SYVYWHGSMP
110 120 130 140 150
GPSQTLLKVS TSVGTVIGQF GFGTLADIVG RKRIYGMELI IMIVCTILQT
160 170 180 190 200
TVAHSPAINF VAVLTFYRIV MGIGIGGDYP LSSIITSEFA TTKWRGAIMG
210 220 230 240 250
AVFANQAWGQ ISGGIIALIL VAAYKGELEY ANSGAECDAR CQKACDQMWR
260 270 280 290 300
ILIGLGTVLG LACLYFRLTI PESPRYQLDV NAKLELAAAA QEQDGEKKIH
310 320 330 340 350
DTSDEDMAIN GLERASTAVE SLDNHPPKAS FKDFCRHFGQ WKYGKILLGT
360 370 380 390 400
AGSWFTLDVA FYGLSLNSAV ILQTIGYAGS KNVYKKLYDT AVGNLILICA
410 420 430 440 450
GSLPGYWVSV FTVDIIGRKP IQLAGFIILT ALFCVIGFAY HKLGDHGLLA
460 470 480 490 500
LYVICQFFQN FGPNTTTFIV PGECFPTRYR STAHGISAAS GKVGAIIAQT
510 520 530 540 550
ALGTLIDHNC ARDGKPTNCW LPHVMEIFAL FMLLGIFTTL LIPETKRKTL
560 570 580
EEINELYHDE IDPATLNFRN KNNDIESSSP SQLQHEA
Length:587
Mass (Da):64,382
Last modified:February 1, 1996 - v2
Checksum:iE463954395E8A840
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621A → AHSPAINFVA (PubMed:2038328).Curated
Sequence conflicti353 – 3531S → Y in BAA14358 (PubMed:2038328).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90346 Genomic DNA. Translation: BAA14358.1.
Z49218 Genomic DNA. Translation: CAA89157.1.
BK006946 Genomic DNA. Translation: DAA09776.1.
PIRiS54061.
RefSeqiNP_013583.1. NM_001182486.1.

Genome annotation databases

EnsemblFungiiYML123C; YML123C; YML123C.
GeneIDi854916.
KEGGisce:YML123C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90346 Genomic DNA. Translation: BAA14358.1.
Z49218 Genomic DNA. Translation: CAA89157.1.
BK006946 Genomic DNA. Translation: DAA09776.1.
PIRiS54061.
RefSeqiNP_013583.1. NM_001182486.1.

3D structure databases

ProteinModelPortaliP25297.
SMRiP25297. Positions 62-552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35082. 138 interactions.
DIPiDIP-5072N.
IntActiP25297. 2 interactions.
MINTiMINT-498423.
STRINGi4932.YML123C.

Protein family/group databases

TCDBi2.A.1.9.1. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBiP25297.
PaxDbiP25297.
PeptideAtlasiP25297.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML123C; YML123C; YML123C.
GeneIDi854916.
KEGGisce:YML123C.

Organism-specific databases

CYGDiYML123c.
EuPathDBiFungiDB:YML123C.
SGDiS000004592. PHO84.

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00750000118743.
HOGENOMiHOG000171119.
InParanoidiP25297.
KOiK08176.
OMAiVTSAKCE.
OrthoDBiEOG7RNK80.

Enzyme and pathway databases

BioCyciYEAST:G3O-32702-MONOMER.
BRENDAi3.6.3.27. 984.

Miscellaneous databases

NextBioi977923.
PROiP25297.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR004738. Phos_permease.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00887. 2A0109. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter."
    Bun-Ya M., Nishimura M., Harashima S., Oshima Y.
    Mol. Cell. Biol. 11:3229-3238(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-6 AND LYS-298.
    Strain: SUB592.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302; SER-303; SER-316; THR-317; SER-321; SER-577; SER-579 AND SER-581, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302; SER-303; SER-316; THR-317; SER-321; SER-579 AND SER-581, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPHO84_YEAST
AccessioniPrimary (citable) accession number: P25297
Secondary accession number(s): D6W0G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 335000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.