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P25296 (CANB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcineurin subunit B
Alternative name(s):
Calcineurin regulatory subunit
Protein phosphatase 2B regulatory subunit
Gene names
Name:CNB1
Synonyms:YCN2, YCNB
Ordered Locus Names:YKL190W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Subunit structure

Composed of a catalytic subunit (A) and a regulatory subunit (B).

Miscellaneous

This protein has four functional calcium-binding sites.

Sequence similarities

Belongs to the calcineurin regulatory subunit family.

Contains 4 EF-hand domains.

Sequence caution

The sequence CAA52248.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CMP2P147473EBI-3968,EBI-12778
YAP1P198802EBI-3968,EBI-31265

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 175174Calcineurin subunit B
PRO_0000073498

Regions

Domain21 – 5636EF-hand 1
Domain53 – 8836EF-hand 2
Domain90 – 12536EF-hand 3
Domain131 – 16636EF-hand 4
Calcium binding34 – 45121 By similarity
Calcium binding66 – 77122 By similarity
Calcium binding103 – 114123 By similarity
Calcium binding144 – 155124 By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine Ref.2

Sequences

Sequence LengthMass (Da)Tools
P25296 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 6E42DB350DA05BC0

FASTA17519,639
        10         20         30         40         50         60 
MGAAPSKIVD GLLEDTNFDR DEIERLRKRF MKLDRDSSGS IDKNEFMSIP GVSSNPLAGR 

        70         80         90        100        110        120 
IMEVFDADNS GDVDFQEFIT GLSIFSGRGS KDEKLRFAFK IYDIDKDGFI SNGELFIVLK 

       130        140        150        160        170 
IMVGSNLDDE QLQQIVDRTI VENDSDGDGR LSFEEFKNAI ETTEVAKSLT LQYDV 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning of a calcineurin B homolog in Saccharomyces cerevisiae."
Kuno T., Tanaka H., Mukai H., Chang C.-D., Hiraga K., Miyakawa T., Tanaka C.
Biochem. Biophys. Res. Commun. 180:1159-1163(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ATCC 204626 / S288c / A364A.
[2]"Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone."
Cyert M.S., Thorner J.
Mol. Cell. Biol. 12:3460-3469(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MYRISTOYLATION AT GLY-2.
[3]"DNA sequence analysis of the YCN2 region of chromosome XI in Saccharomyces cerevisiae."
Cheret G., Mattheakis L.C., Sor F.
Yeast 9:661-667(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[4]"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z26521 Genomic DNA. Translation: CAA81290.1.
D10293 mRNA. Translation: BAA01136.1.
M87508 Genomic DNA. Translation: AAA34505.1.
X69765 Genomic DNA. Translation: CAA49421.1.
X74151 Genomic DNA. Translation: CAA52248.1. Different initiation.
Z28190 Genomic DNA. Translation: CAA82034.1.
Z28189 Genomic DNA. Translation: CAA82033.1.
BK006944 Genomic DNA. Translation: DAA08976.1.
PIRJH0462.
RefSeqNP_012731.1. NM_001179756.1.

3D structure databases

ProteinModelPortalP25296.
SMRP25296. Positions 19-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33931. 220 interactions.
DIPDIP-510N.
IntActP25296. 13 interactions.
MINTMINT-409037.
STRING4932.YKL190W.

Proteomic databases

MaxQBP25296.
PaxDbP25296.
PeptideAtlasP25296.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL190W; YKL190W; YKL190W.
GeneID853644.
KEGGsce:YKL190W.

Organism-specific databases

SGDS000001673. CNB1.

Phylogenomic databases

eggNOGCOG5126.
GeneTreeENSGT00750000117505.
HOGENOMHOG000233019.
KOK06268.
OMATILFADK.
OrthoDBEOG7PK99H.

Enzyme and pathway databases

BioCycYEAST:G3O-31953-MONOMER.

Gene expression databases

GenevestigatorP25296.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTSM00054. EFh. 4 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio974543.
PROP25296.

Entry information

Entry nameCANB_YEAST
AccessionPrimary (citable) accession number: P25296
Secondary accession number(s): D6VX10
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families