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P25296

- CANB_YEAST

UniProt

P25296 - CANB_YEAST

Protein

Calcineurin subunit B

Gene

CNB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi34 – 45121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi66 – 77122PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi103 – 114123PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi144 – 155124PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
    2. cellular ion homeostasis Source: SGD
    3. dephosphorylation Source: GOC
    4. fungal-type cell wall organization Source: SGD

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31953-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcineurin subunit B
    Alternative name(s):
    Calcineurin regulatory subunit
    Protein phosphatase 2B regulatory subunit
    Gene namesi
    Name:CNB1
    Synonyms:YCN2, YCNB
    Ordered Locus Names:YKL190W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    SGDiS000001673. CNB1.

    Subcellular locationi

    GO - Cellular componenti

    1. calcineurin complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 175174Calcineurin subunit BPRO_0000073498Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine1 Publication

    Keywords - PTMi

    Lipoprotein, Myristate

    Proteomic databases

    MaxQBiP25296.
    PaxDbiP25296.
    PeptideAtlasiP25296.

    Expressioni

    Gene expression databases

    GenevestigatoriP25296.

    Interactioni

    Subunit structurei

    Composed of a catalytic subunit (A) and a regulatory subunit (B).

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CMP2P147473EBI-3968,EBI-12778
    YAP1P198802EBI-3968,EBI-31265

    Protein-protein interaction databases

    BioGridi33931. 221 interactions.
    DIPiDIP-510N.
    IntActiP25296. 13 interactions.
    MINTiMINT-409037.
    STRINGi4932.YKL190W.

    Structurei

    3D structure databases

    ProteinModelPortaliP25296.
    SMRiP25296. Positions 19-156.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 5636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini53 – 8836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini90 – 12536EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini131 – 16636EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    GeneTreeiENSGT00750000117505.
    HOGENOMiHOG000233019.
    KOiK06268.
    OMAiTILFADK.
    OrthoDBiEOG7PK99H.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 2 hits.
    [Graphical view]
    SMARTiSM00054. EFh. 4 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25296-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAAPSKIVD GLLEDTNFDR DEIERLRKRF MKLDRDSSGS IDKNEFMSIP    50
    GVSSNPLAGR IMEVFDADNS GDVDFQEFIT GLSIFSGRGS KDEKLRFAFK 100
    IYDIDKDGFI SNGELFIVLK IMVGSNLDDE QLQQIVDRTI VENDSDGDGR 150
    LSFEEFKNAI ETTEVAKSLT LQYDV 175
    Length:175
    Mass (Da):19,639
    Last modified:January 23, 2007 - v3
    Checksum:i6E42DB350DA05BC0
    GO

    Sequence cautioni

    The sequence CAA52248.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26521 Genomic DNA. Translation: CAA81290.1.
    D10293 mRNA. Translation: BAA01136.1.
    M87508 Genomic DNA. Translation: AAA34505.1.
    X69765 Genomic DNA. Translation: CAA49421.1.
    X74151 Genomic DNA. Translation: CAA52248.1. Different initiation.
    Z28190 Genomic DNA. Translation: CAA82034.1.
    Z28189 Genomic DNA. Translation: CAA82033.1.
    BK006944 Genomic DNA. Translation: DAA08976.1.
    PIRiJH0462.
    RefSeqiNP_012731.1. NM_001179756.1.

    Genome annotation databases

    EnsemblFungiiYKL190W; YKL190W; YKL190W.
    GeneIDi853644.
    KEGGisce:YKL190W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26521 Genomic DNA. Translation: CAA81290.1 .
    D10293 mRNA. Translation: BAA01136.1 .
    M87508 Genomic DNA. Translation: AAA34505.1 .
    X69765 Genomic DNA. Translation: CAA49421.1 .
    X74151 Genomic DNA. Translation: CAA52248.1 . Different initiation.
    Z28190 Genomic DNA. Translation: CAA82034.1 .
    Z28189 Genomic DNA. Translation: CAA82033.1 .
    BK006944 Genomic DNA. Translation: DAA08976.1 .
    PIRi JH0462.
    RefSeqi NP_012731.1. NM_001179756.1.

    3D structure databases

    ProteinModelPortali P25296.
    SMRi P25296. Positions 19-156.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33931. 221 interactions.
    DIPi DIP-510N.
    IntActi P25296. 13 interactions.
    MINTi MINT-409037.
    STRINGi 4932.YKL190W.

    Proteomic databases

    MaxQBi P25296.
    PaxDbi P25296.
    PeptideAtlasi P25296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL190W ; YKL190W ; YKL190W .
    GeneIDi 853644.
    KEGGi sce:YKL190W.

    Organism-specific databases

    SGDi S000001673. CNB1.

    Phylogenomic databases

    eggNOGi COG5126.
    GeneTreei ENSGT00750000117505.
    HOGENOMi HOG000233019.
    KOi K06268.
    OMAi TILFADK.
    OrthoDBi EOG7PK99H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31953-MONOMER.

    Miscellaneous databases

    NextBioi 974543.
    PROi P25296.

    Gene expression databases

    Genevestigatori P25296.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 2 hits.
    [Graphical view ]
    SMARTi SM00054. EFh. 4 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ATCC 204626 / S288c / A364A.
    2. "Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone."
      Cyert M.S., Thorner J.
      Mol. Cell. Biol. 12:3460-3469(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MYRISTOYLATION AT GLY-2.
    3. "DNA sequence analysis of the YCN2 region of chromosome XI in Saccharomyces cerevisiae."
      Cheret G., Mattheakis L.C., Sor F.
      Yeast 9:661-667(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    4. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
      Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
      Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiCANB_YEAST
    AccessioniPrimary (citable) accession number: P25296
    Secondary accession number(s): D6VX10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein has four functional calcium-binding sites.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3