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P25296

- CANB_YEAST

UniProt

P25296 - CANB_YEAST

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Protein

Calcineurin subunit B

Gene
CNB1, YCN2, YCNB, YKL190W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi34 – 45121 By similarityAdd
BLAST
Calcium bindingi66 – 77122 By similarityAdd
BLAST
Calcium bindingi103 – 114123 By similarityAdd
BLAST
Calcium bindingi144 – 155124 By similarityAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
  2. cellular ion homeostasis Source: SGD
  3. dephosphorylation Source: GOC
  4. fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit B
Alternative name(s):
Calcineurin regulatory subunit
Protein phosphatase 2B regulatory subunit
Gene namesi
Name:CNB1
Synonyms:YCN2, YCNB
Ordered Locus Names:YKL190W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

SGDiS000001673. CNB1.

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 175174Calcineurin subunit BPRO_0000073498Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiP25296.
PaxDbiP25296.
PeptideAtlasiP25296.

Expressioni

Gene expression databases

GenevestigatoriP25296.

Interactioni

Subunit structurei

Composed of a catalytic subunit (A) and a regulatory subunit (B).

Binary interactionsi

WithEntry#Exp.IntActNotes
CMP2P147473EBI-3968,EBI-12778
YAP1P198802EBI-3968,EBI-31265

Protein-protein interaction databases

BioGridi33931. 220 interactions.
DIPiDIP-510N.
IntActiP25296. 13 interactions.
MINTiMINT-409037.
STRINGi4932.YKL190W.

Structurei

3D structure databases

ProteinModelPortaliP25296.
SMRiP25296. Positions 19-156.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 5636EF-hand 1Add
BLAST
Domaini53 – 8836EF-hand 2Add
BLAST
Domaini90 – 12536EF-hand 3Add
BLAST
Domaini131 – 16636EF-hand 4Add
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00750000117505.
HOGENOMiHOG000233019.
KOiK06268.
OMAiTILFADK.
OrthoDBiEOG7PK99H.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25296-1 [UniParc]FASTAAdd to Basket

« Hide

MGAAPSKIVD GLLEDTNFDR DEIERLRKRF MKLDRDSSGS IDKNEFMSIP    50
GVSSNPLAGR IMEVFDADNS GDVDFQEFIT GLSIFSGRGS KDEKLRFAFK 100
IYDIDKDGFI SNGELFIVLK IMVGSNLDDE QLQQIVDRTI VENDSDGDGR 150
LSFEEFKNAI ETTEVAKSLT LQYDV 175
Length:175
Mass (Da):19,639
Last modified:January 23, 2007 - v3
Checksum:i6E42DB350DA05BC0
GO

Sequence cautioni

The sequence CAA52248.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z26521 Genomic DNA. Translation: CAA81290.1.
D10293 mRNA. Translation: BAA01136.1.
M87508 Genomic DNA. Translation: AAA34505.1.
X69765 Genomic DNA. Translation: CAA49421.1.
X74151 Genomic DNA. Translation: CAA52248.1. Different initiation.
Z28190 Genomic DNA. Translation: CAA82034.1.
Z28189 Genomic DNA. Translation: CAA82033.1.
BK006944 Genomic DNA. Translation: DAA08976.1.
PIRiJH0462.
RefSeqiNP_012731.1. NM_001179756.1.

Genome annotation databases

EnsemblFungiiYKL190W; YKL190W; YKL190W.
GeneIDi853644.
KEGGisce:YKL190W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z26521 Genomic DNA. Translation: CAA81290.1 .
D10293 mRNA. Translation: BAA01136.1 .
M87508 Genomic DNA. Translation: AAA34505.1 .
X69765 Genomic DNA. Translation: CAA49421.1 .
X74151 Genomic DNA. Translation: CAA52248.1 . Different initiation.
Z28190 Genomic DNA. Translation: CAA82034.1 .
Z28189 Genomic DNA. Translation: CAA82033.1 .
BK006944 Genomic DNA. Translation: DAA08976.1 .
PIRi JH0462.
RefSeqi NP_012731.1. NM_001179756.1.

3D structure databases

ProteinModelPortali P25296.
SMRi P25296. Positions 19-156.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33931. 220 interactions.
DIPi DIP-510N.
IntActi P25296. 13 interactions.
MINTi MINT-409037.
STRINGi 4932.YKL190W.

Proteomic databases

MaxQBi P25296.
PaxDbi P25296.
PeptideAtlasi P25296.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL190W ; YKL190W ; YKL190W .
GeneIDi 853644.
KEGGi sce:YKL190W.

Organism-specific databases

SGDi S000001673. CNB1.

Phylogenomic databases

eggNOGi COG5126.
GeneTreei ENSGT00750000117505.
HOGENOMi HOG000233019.
KOi K06268.
OMAi TILFADK.
OrthoDBi EOG7PK99H.

Enzyme and pathway databases

BioCyci YEAST:G3O-31953-MONOMER.

Miscellaneous databases

NextBioi 974543.
PROi P25296.

Gene expression databases

Genevestigatori P25296.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF13499. EF-hand_7. 2 hits.
[Graphical view ]
SMARTi SM00054. EFh. 4 hits.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ATCC 204626 / S288c / A364A.
  2. "Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone."
    Cyert M.S., Thorner J.
    Mol. Cell. Biol. 12:3460-3469(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MYRISTOYLATION AT GLY-2.
  3. "DNA sequence analysis of the YCN2 region of chromosome XI in Saccharomyces cerevisiae."
    Cheret G., Mattheakis L.C., Sor F.
    Yeast 9:661-667(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  4. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
    Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
    Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiCANB_YEAST
AccessioniPrimary (citable) accession number: P25296
Secondary accession number(s): D6VX10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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