P25296 (CANB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcineurin subunit B

Alternative name(s):
Calcineurin regulatory subunit
Protein phosphatase 2B regulatory subunit

Gene names

Name:	CNB1
Synonyms:	YCN2, YCNB
Ordered Locus Names:	YKL190W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Subunit structure	Composed of a catalytic subunit (A) and a regulatory subunit (B).
Miscellaneous	This protein has four functional calcium-binding sites.
Sequence similarities	Belongs to the calcineurin regulatory subunit family. Contains 4 EF-hand domains.
Sequence caution	The sequence CAA52248.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.2. Source: SGD cellular ion homeostasis Inferred from mutant phenotype. Source: SGD fungal-type cell wall organization Inferred from genetic interaction. Source: SGD
Cellular component	calcineurin complex Inferred from direct assay Ref.2. Source: SGD
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CMP2	P14747	6	EBI-3968,EBI-12778

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 175	174	Calcineurin subunit B		PRO_0000073498
Regions
	Domain	21 – 56	36	EF-hand 1
	Domain	53 – 88	36	EF-hand 2
	Domain	90 – 125	36	EF-hand 3
	Domain	131 – 166	36	EF-hand 4
	Calcium binding	34 – 45	12	1 By similarity
	Calcium binding	66 – 77	12	2 By similarity
	Calcium binding	103 – 114	12	3 By similarity
	Calcium binding	144 – 155	12	4 By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P25296 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6E42DB350DA05BC0	FASTA	175	19,639
	10 20 30 40 50 60 MGAAPSKIVD GLLEDTNFDR DEIERLRKRF MKLDRDSSGS IDKNEFMSIP GVSSNPLAGR 70 80 90 100 110 120 IMEVFDADNS GDVDFQEFIT GLSIFSGRGS KDEKLRFAFK IYDIDKDGFI SNGELFIVLK 130 140 150 160 170 IMVGSNLDDE QLQQIVDRTI VENDSDGDGR LSFEEFKNAI ETTEVAKSLT LQYDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of a calcineurin B homolog in Saccharomyces cerevisiae." Kuno T., Tanaka H., Mukai H., Chang C.-D., Hiraga K., Miyakawa T., Tanaka C. Biochem. Biophys. Res. Commun. 180:1159-1163(1991) [PubMed: 1659397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 204626 / S288c / A364A.
[2]	"Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone." Cyert M.S., Thorner J. Mol. Cell. Biol. 12:3460-3469(1992) [PubMed: 1321337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MYRISTOYLATION AT GLY-2.
[3]	"DNA sequence analysis of the YCN2 region of chromosome XI in Saccharomyces cerevisiae." Cheret G., Mattheakis L.C., Sor F. Yeast 9:661-667(1993) [PubMed: 8394042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[4]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed: 8154185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26521 Genomic DNA. Translation: CAA81290.1. D10293 mRNA. Translation: BAA01136.1. M87508 Genomic DNA. Translation: AAA34505.1. X69765 Genomic DNA. Translation: CAA49421.1. X74151 Genomic DNA. Translation: CAA52248.1. Different initiation. Z28190 Genomic DNA. Translation: CAA82034.1. Z28189 Genomic DNA. Translation: CAA82033.1. BK006944 Genomic DNA. Translation: DAA08976.1.
PIR	JH0462.
RefSeq	NP_012731.1. NM_001179756.1.
3D structure databases
ProteinModelPortal	P25296.
SMR	P25296. Positions 19-156.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-510N.
IntAct	P25296. 13 interactions.
MINT	MINT-409037.
STRING	P25296.
Proteomic databases
PeptideAtlas	P25296.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL190W; YKL190W; YKL190W.
GeneID	853644.
KEGG	sce:YKL190W.
NMPDR	fig|4932.3.peg.3708.
Organism-specific databases
SGD	S000001673. CNB1.
Phylogenomic databases
eggNOG	fuNOG05219.
GeneTree	EFGT00050000003659.
HOGENOM	HBG746798.
OMA	IYDIDKD.
OrthoDB	EOG4HHSBR.
Gene expression databases
ArrayExpress	P25296.
Genevestigator	P25296.
GermOnline	YKL190W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR008080. Parvalbumin. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
KO	K06268.
PRINTS	PR01697. PARVALBUMIN.
SMART	SM00054. EFh. 4 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 4 hits. PS50222. EF_HAND_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	974543.

Entry information

Entry name

CANB_YEAST

Accession

Primary (citable) accession number: P25296
Secondary accession number(s): D6VX10

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program