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P25294 (SIS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein SIS1
Gene names
Name:SIS1
Ordered Locus Names:YNL007C
ORF Names:N2879
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for nuclear migration during mitosis. It is required for the normal initiation of translation. Might mediate the dissociation of a specific protein complex of the translation machinery. Essential for viability.

Subunit structure

Interacts with polyadenylate-binding protein PAB1. Ref.5

Subcellular location

Cytoplasm. Nucleus. Note: Localized throughout the cell but is more concentrated at the nucleus. Ref.6

Miscellaneous

Present with 20300 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 J domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SSA1P105913EBI-17244,EBI-8591
SUP35Q7LKB13EBI-17244,EBI-8411471From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352Protein SIS1
PRO_0000071090

Regions

Domain4 – 7067J
Compositional bias75 – 16692Gly-rich

Amino acid modifications

Modified residue2751Phosphoserine Ref.9

Secondary structure

............................................... 352
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25294 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 642D3709C0FD5682

FASTA35237,590
        10         20         30         40         50         60 
MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE AFEILNDPQK 

        70         80         90        100        110        120 
REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF SGGHAFSNED AFNIFSQFFG 

       130        140        150        160        170        180 
GSSPFGGADD SGFSFSSYPS GGGAGMGGMP GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE 

       190        200        210        220        230        240 
TVQVNLPVSL EDLFVGKKKS FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN 

       250        260        270        280        290        300 
PQTGRRKTLQ FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV 

       310        320        330        340        350 
QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE NF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of SIS1, a Saccharomyces cerevisiae homologue of bacterial dnaJ proteins."
Luke M.M., Sutton A., Arndt K.T.
J. Cell Biol. 114:623-638(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation."
Zhong T., Arndt K.T.
Cell 73:1175-1186(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"The yeast hsp70 homologue Ssa is required for translation and interacts with Sis1 and Pab1 on translating ribosomes."
Horton L.E., James P., Craig E.A., Hensold J.O.
J. Biol. Chem. 276:14426-14433(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SSA1.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58460 Genomic DNA. Translation: CAA41366.1.
Z71283 Genomic DNA. Translation: CAA95866.1.
BK006947 Genomic DNA. Translation: DAA10536.1.
PIRA39660.
RefSeqNP_014391.1. NM_001182846.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1C3GX-ray2.70A180-349[»]
2B26X-ray3.20A/B/C181-352[»]
2O37X-ray1.25A1-89[»]
ProteinModelPortalP25294.
SMRP25294. Positions 1-81, 180-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35818. 70 interactions.
DIPDIP-4385N.
IntActP25294. 19 interactions.
MINTMINT-472083.
STRING4932.YNL007C.

Proteomic databases

MaxQBP25294.
PaxDbP25294.
PeptideAtlasP25294.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL007C; YNL007C; YNL007C.
GeneID855725.
KEGGsce:YNL007C.

Organism-specific databases

CYGDYNL007c.
SGDS000004952. SIS1.

Phylogenomic databases

eggNOGCOG0484.
GeneTreeENSGT00730000110742.
HOGENOMHOG000226718.
OMAYKVDYPI.
OrthoDBEOG7SFJ7D.

Enzyme and pathway databases

BioCycYEAST:G3O-33049-MONOMER.

Gene expression databases

GenevestigatorP25294.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
InterProIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP25294.
NextBio980096.
PROP25294.

Entry information

Entry nameSIS1_YEAST
AccessionPrimary (citable) accession number: P25294
Secondary accession number(s): D6W1H0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references