Protein SIS1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P25294 (SIS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 131. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein SIS1

Gene names

Name:	SIS1
Ordered Locus Names:	YNL007C
ORF Names:	N2879

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for nuclear migration during mitosis. It is required for the normal initiation of translation. Might mediate the dissociation of a specific protein complex of the translation machinery. Essential for viability.
Subunit structure	Interacts with polyadenylate-binding protein PAB1. Ref.5
Subcellular location	Cytoplasm. Nucleus. Note: Localized throughout the cell but is more concentrated at the nucleus. Ref.6
Miscellaneous	Present with 20300 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from direct assay PubMed 9774392. Source: SGD translational initiation Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay PubMed 22718905. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Traceable author statement PubMed 11923285. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 352

352

Protein SIS1

PRO_0000071090

Regions

Domain

4 – 70

Compositional bias

75 – 166

Gly-rich

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

173

Phosphoserine Ref.8 Ref.9

Secondary structure

352

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P25294 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 642D3709C0FD5682
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FASTA

352

37,590

        10         20         30         40         50         60 
MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE AFEILNDPQK 

        70         80         90        100        110        120 
REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF SGGHAFSNED AFNIFSQFFG 

       130        140        150        160        170        180 
GSSPFGGADD SGFSFSSYPS GGGAGMGGMP GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE 

       190        200        210        220        230        240 
TVQVNLPVSL EDLFVGKKKS FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN 

       250        260        270        280        290        300 
PQTGRRKTLQ FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV 

       310        320        330        340        350 
QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE NF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of SIS1, a Saccharomyces cerevisiae homologue of bacterial dnaJ proteins." Luke M.M., Sutton A., Arndt K.T. J. Cell Biol. 114:623-638(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation." Zhong T., Arndt K.T. Cell 73:1175-1186(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"The yeast hsp70 homologue Ssa is required for translation and interacts with Sis1 and Pab1 on translating ribosomes." Horton L.E., James P., Craig E.A., Hensold J.O. J. Biol. Chem. 276:14426-14433(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SSA1.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 AND SER-173, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X58460 Genomic DNA. Translation: CAA41366.1.
Z71283 Genomic DNA. Translation: CAA95866.1.
BK006947 Genomic DNA. Translation: DAA10536.1.

PIR

A39660.

RefSeq

NP_014391.1. NM_001182846.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C3G	X-ray	2.70	A	180-349	[»]
2B26	X-ray	3.20	A/B/C	181-352	[»]
2O37	X-ray	1.25	A	1-89	[»]

ProteinModelPortal

P25294.

SMR

P25294. Positions 1-81, 180-349.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4385N.

IntAct

P25294. 18 interactions.

MINT

MINT-472083.

STRING

4932.YNL007C.

Proteomic databases

PaxDb

P25294.

PeptideAtlas

P25294.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YNL007C; YNL007C; YNL007C.

GeneID

855725.

KEGG

sce:YNL007C.

Organism-specific databases

CYGD

YNL007c.

SGD

S000004952. SIS1.

Phylogenomic databases

eggNOG

COG0484.

GeneTree

ENSGT00690000102068.

HOGENOM

HOG000226718.

OMA

SFFQIEG.

OrthoDB

EOG480N5S.

Gene expression databases

Genevestigator

P25294.

GermOnline

YNL007C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.287.110. 1 hit.

InterPro

IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]

Pfam

PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]

PRINTS

PR00625. JDOMAIN.

SMART

SM00271. DnaJ. 1 hit.
[Graphical view]

SUPFAM

SSF46565. DnaJ_N. 1 hit.
SSF49493. HSP40_DnaJ_pep. 2 hits.

PROSITE

PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P25294.

NextBio

980096.

Entry information

Entry name

SIS1_YEAST

Accession

Primary (citable) accession number: P25294
Secondary accession number(s): D6W1H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents