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P25294

- SIS1_YEAST

UniProt

P25294 - SIS1_YEAST

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Protein

Protein SIS1

Gene

SIS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for nuclear migration during mitosis. It is required for the normal initiation of translation. Might mediate the dissociation of a specific protein complex of the translation machinery. Essential for viability.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. misfolded protein binding Source: SGD

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. misfolded protein transport Source: SGD
  3. nucleus-associated proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  4. protein folding Source: SGD
  5. translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33049-MONOMER.
ReactomeiREACT_205607. Regulation of HSF1-mediated heat shock response.
REACT_219346. HSF1-dependent transactivation.
REACT_271186. Attenuation phase.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SIS1
Gene namesi
Name:SIS1
Ordered Locus Names:YNL007C
ORF Names:N2879
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL007c.
SGDiS000004952. SIS1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: Localized throughout the cell but is more concentrated at the nucleus.

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Protein SIS1PRO_0000071090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei275 – 2751Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25294.
PaxDbiP25294.
PeptideAtlasiP25294.

Expressioni

Gene expression databases

GenevestigatoriP25294.

Interactioni

Subunit structurei

Interacts with polyadenylate-binding protein PAB1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA1P105913EBI-17244,EBI-8591
SUP35Q7LKB13EBI-17244,EBI-8411471From a different organism.

Protein-protein interaction databases

BioGridi35818. 71 interactions.
DIPiDIP-4385N.
IntActiP25294. 19 interactions.
MINTiMINT-472083.
STRINGi4932.YNL007C.

Structurei

Secondary structure

1
352
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 116Combined sources
Helixi19 – 3315Combined sources
Helixi42 – 5514Combined sources
Helixi58 – 6710Combined sources
Helixi69 – 735Combined sources
Beta strandi181 – 1888Combined sources
Helixi190 – 1956Combined sources
Beta strandi198 – 20710Combined sources
Turni208 – 2103Combined sources
Beta strandi211 – 22010Combined sources
Beta strandi230 – 2356Combined sources
Beta strandi237 – 2393Combined sources
Beta strandi241 – 2444Combined sources
Beta strandi248 – 2558Combined sources
Beta strandi259 – 2646Combined sources
Beta strandi267 – 2726Combined sources
Helixi276 – 2816Combined sources
Beta strandi283 – 2886Combined sources
Beta strandi290 – 2923Combined sources
Beta strandi294 – 3018Combined sources
Beta strandi308 – 3103Combined sources
Beta strandi319 – 3213Combined sources
Beta strandi328 – 3325Combined sources
Helixi344 – 3474Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C3GX-ray2.70A180-349[»]
2B26X-ray3.20A/B/C181-352[»]
2O37X-ray1.25A1-89[»]
ProteinModelPortaliP25294.
SMRiP25294. Positions 1-81, 180-349.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25294.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 7067JPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 16692Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0484.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000226718.
InParanoidiP25294.
OMAiYKVDYPI.
OrthoDBiEOG7SFJ7D.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25294-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE
60 70 80 90 100
AFEILNDPQK REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF
110 120 130 140 150
SGGHAFSNED AFNIFSQFFG GSSPFGGADD SGFSFSSYPS GGGAGMGGMP
160 170 180 190 200
GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE TVQVNLPVSL EDLFVGKKKS
210 220 230 240 250
FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN PQTGRRKTLQ
260 270 280 290 300
FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV
310 320 330 340 350
QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE

NF
Length:352
Mass (Da):37,590
Last modified:May 1, 1992 - v1
Checksum:i642D3709C0FD5682
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58460 Genomic DNA. Translation: CAA41366.1.
Z71283 Genomic DNA. Translation: CAA95866.1.
BK006947 Genomic DNA. Translation: DAA10536.1.
PIRiA39660.
RefSeqiNP_014391.1. NM_001182846.1.

Genome annotation databases

EnsemblFungiiYNL007C; YNL007C; YNL007C.
GeneIDi855725.
KEGGisce:YNL007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58460 Genomic DNA. Translation: CAA41366.1 .
Z71283 Genomic DNA. Translation: CAA95866.1 .
BK006947 Genomic DNA. Translation: DAA10536.1 .
PIRi A39660.
RefSeqi NP_014391.1. NM_001182846.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1C3G X-ray 2.70 A 180-349 [» ]
2B26 X-ray 3.20 A/B/C 181-352 [» ]
2O37 X-ray 1.25 A 1-89 [» ]
ProteinModelPortali P25294.
SMRi P25294. Positions 1-81, 180-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35818. 71 interactions.
DIPi DIP-4385N.
IntActi P25294. 19 interactions.
MINTi MINT-472083.
STRINGi 4932.YNL007C.

Proteomic databases

MaxQBi P25294.
PaxDbi P25294.
PeptideAtlasi P25294.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL007C ; YNL007C ; YNL007C .
GeneIDi 855725.
KEGGi sce:YNL007C.

Organism-specific databases

CYGDi YNL007c.
SGDi S000004952. SIS1.

Phylogenomic databases

eggNOGi COG0484.
GeneTreei ENSGT00760000118947.
HOGENOMi HOG000226718.
InParanoidi P25294.
OMAi YKVDYPI.
OrthoDBi EOG7SFJ7D.

Enzyme and pathway databases

BioCyci YEAST:G3O-33049-MONOMER.
Reactomei REACT_205607. Regulation of HSF1-mediated heat shock response.
REACT_219346. HSF1-dependent transactivation.
REACT_271186. Attenuation phase.

Miscellaneous databases

EvolutionaryTracei P25294.
NextBioi 980096.
PROi P25294.

Gene expression databases

Genevestigatori P25294.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
InterProi IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of SIS1, a Saccharomyces cerevisiae homologue of bacterial dnaJ proteins."
    Luke M.M., Sutton A., Arndt K.T.
    J. Cell Biol. 114:623-638(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation."
    Zhong T., Arndt K.T.
    Cell 73:1175-1186(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The yeast hsp70 homologue Ssa is required for translation and interacts with Sis1 and Pab1 on translating ribosomes."
    Horton L.E., James P., Craig E.A., Hensold J.O.
    J. Biol. Chem. 276:14426-14433(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SSA1.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSIS1_YEAST
AccessioniPrimary (citable) accession number: P25294
Secondary accession number(s): D6W1H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 26, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 20300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3