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P25294

- SIS1_YEAST

UniProt

P25294 - SIS1_YEAST

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Protein

Protein SIS1

Gene
SIS1, YNL007C, N2879
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for nuclear migration during mitosis. It is required for the normal initiation of translation. Might mediate the dissociation of a specific protein complex of the translation machinery. Essential for viability.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. misfolded protein binding Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. misfolded protein transport Source: SGD
  3. nucleus-associated proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  4. protein folding Source: SGD
  5. translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33049-MONOMER.
ReactomeiREACT_219346. HSF1-dependent transactivation.
REACT_221137. Attenuation phase.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SIS1
Gene namesi
Name:SIS1
Ordered Locus Names:YNL007C
ORF Names:N2879
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL007c.
SGDiS000004952. SIS1.

Subcellular locationi

Cytoplasm. Nucleus
Note: Localized throughout the cell but is more concentrated at the nucleus.1 Publication

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Protein SIS1PRO_0000071090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei275 – 2751Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25294.
PaxDbiP25294.
PeptideAtlasiP25294.

Expressioni

Gene expression databases

GenevestigatoriP25294.

Interactioni

Subunit structurei

Interacts with polyadenylate-binding protein PAB1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA1P105913EBI-17244,EBI-8591
SUP35Q7LKB13EBI-17244,EBI-8411471From a different organism.

Protein-protein interaction databases

BioGridi35818. 70 interactions.
DIPiDIP-4385N.
IntActiP25294. 19 interactions.
MINTiMINT-472083.
STRINGi4932.YNL007C.

Structurei

Secondary structure

1
352
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 116
Helixi19 – 3315
Helixi42 – 5514
Helixi58 – 6710
Helixi69 – 735
Beta strandi181 – 1888
Helixi190 – 1956
Beta strandi198 – 20710
Turni208 – 2103
Beta strandi211 – 22010
Beta strandi230 – 2356
Beta strandi237 – 2393
Beta strandi241 – 2444
Beta strandi248 – 2558
Beta strandi259 – 2646
Beta strandi267 – 2726
Helixi276 – 2816
Beta strandi283 – 2886
Beta strandi290 – 2923
Beta strandi294 – 3018
Beta strandi308 – 3103
Beta strandi319 – 3213
Beta strandi328 – 3325
Helixi344 – 3474

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C3GX-ray2.70A180-349[»]
2B26X-ray3.20A/B/C181-352[»]
2O37X-ray1.25A1-89[»]
ProteinModelPortaliP25294.
SMRiP25294. Positions 1-81, 180-349.

Miscellaneous databases

EvolutionaryTraceiP25294.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 7067JAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 16692Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.

Phylogenomic databases

eggNOGiCOG0484.
GeneTreeiENSGT00730000110742.
HOGENOMiHOG000226718.
OMAiYKVDYPI.
OrthoDBiEOG7SFJ7D.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25294-1 [UniParc]FASTAAdd to Basket

« Hide

MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE    50
AFEILNDPQK REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF 100
SGGHAFSNED AFNIFSQFFG GSSPFGGADD SGFSFSSYPS GGGAGMGGMP 150
GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE TVQVNLPVSL EDLFVGKKKS 200
FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN PQTGRRKTLQ 250
FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV 300
QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE 350
NF 352
Length:352
Mass (Da):37,590
Last modified:May 1, 1992 - v1
Checksum:i642D3709C0FD5682
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58460 Genomic DNA. Translation: CAA41366.1.
Z71283 Genomic DNA. Translation: CAA95866.1.
BK006947 Genomic DNA. Translation: DAA10536.1.
PIRiA39660.
RefSeqiNP_014391.1. NM_001182846.1.

Genome annotation databases

EnsemblFungiiYNL007C; YNL007C; YNL007C.
GeneIDi855725.
KEGGisce:YNL007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58460 Genomic DNA. Translation: CAA41366.1 .
Z71283 Genomic DNA. Translation: CAA95866.1 .
BK006947 Genomic DNA. Translation: DAA10536.1 .
PIRi A39660.
RefSeqi NP_014391.1. NM_001182846.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1C3G X-ray 2.70 A 180-349 [» ]
2B26 X-ray 3.20 A/B/C 181-352 [» ]
2O37 X-ray 1.25 A 1-89 [» ]
ProteinModelPortali P25294.
SMRi P25294. Positions 1-81, 180-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35818. 70 interactions.
DIPi DIP-4385N.
IntActi P25294. 19 interactions.
MINTi MINT-472083.
STRINGi 4932.YNL007C.

Proteomic databases

MaxQBi P25294.
PaxDbi P25294.
PeptideAtlasi P25294.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL007C ; YNL007C ; YNL007C .
GeneIDi 855725.
KEGGi sce:YNL007C.

Organism-specific databases

CYGDi YNL007c.
SGDi S000004952. SIS1.

Phylogenomic databases

eggNOGi COG0484.
GeneTreei ENSGT00730000110742.
HOGENOMi HOG000226718.
OMAi YKVDYPI.
OrthoDBi EOG7SFJ7D.

Enzyme and pathway databases

BioCyci YEAST:G3O-33049-MONOMER.
Reactomei REACT_219346. HSF1-dependent transactivation.
REACT_221137. Attenuation phase.

Miscellaneous databases

EvolutionaryTracei P25294.
NextBioi 980096.
PROi P25294.

Gene expression databases

Genevestigatori P25294.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
InterProi IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of SIS1, a Saccharomyces cerevisiae homologue of bacterial dnaJ proteins."
    Luke M.M., Sutton A., Arndt K.T.
    J. Cell Biol. 114:623-638(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation."
    Zhong T., Arndt K.T.
    Cell 73:1175-1186(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The yeast hsp70 homologue Ssa is required for translation and interacts with Sis1 and Pab1 on translating ribosomes."
    Horton L.E., James P., Craig E.A., Hensold J.O.
    J. Biol. Chem. 276:14426-14433(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SSA1.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSIS1_YEAST
AccessioniPrimary (citable) accession number: P25294
Secondary accession number(s): D6W1H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 3, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 20300 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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