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Protein

V-type proton ATPase 116 kDa subunit a isoform 1

Gene

Atp6v0a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase 116 kDa subunit a isoform 1
Short name:
V-ATPase 116 kDa isoform a1
Alternative name(s):
Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit
Vacuolar adenosine triphosphatase subunit Ac116
Vacuolar proton pump subunit 1
Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
Gene namesi
Name:Atp6v0a1
Synonyms:Atp6n1, Vpp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68405. Atp6v0a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 388388CytoplasmicSequence analysisAdd
BLAST
Transmembranei389 – 40719HelicalSequence analysisAdd
BLAST
Topological domaini408 – 4092VacuolarSequence analysis
Transmembranei410 – 42617HelicalSequence analysisAdd
BLAST
Topological domaini427 – 44115CytoplasmicSequence analysisAdd
BLAST
Transmembranei442 – 47130HelicalSequence analysisAdd
BLAST
Topological domaini472 – 53564VacuolarSequence analysisAdd
BLAST
Transmembranei536 – 55520HelicalSequence analysisAdd
BLAST
Topological domaini556 – 57318CytoplasmicSequence analysisAdd
BLAST
Transmembranei574 – 59421HelicalSequence analysisAdd
BLAST
Topological domaini595 – 63945VacuolarSequence analysisAdd
BLAST
Transmembranei640 – 65920HelicalSequence analysisAdd
BLAST
Topological domaini660 – 72566CytoplasmicSequence analysisAdd
BLAST
Transmembranei726 – 75025HelicalSequence analysisAdd
BLAST
Topological domaini751 – 77121VacuolarSequence analysisAdd
BLAST
Transmembranei772 – 81039HelicalSequence analysisAdd
BLAST
Topological domaini811 – 83828CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 838838V-type proton ATPase 116 kDa subunit a isoform 1PRO_0000119214Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei250 – 2501PhosphothreonineBy similarity
Modified residuei364 – 3641PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP25286.
PRIDEiP25286.

PTM databases

iPTMnetiP25286.
PhosphoSiteiP25286.

Interactioni

Subunit structurei

The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''.

Protein-protein interaction databases

BioGridi248368. 2 interactions.
IntActiP25286. 1 interaction.
STRINGi10116.ENSRNOP00000052113.

Family & Domainsi

Sequence similaritiesi

Belongs to the V-ATPase 116 kDa subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
HOVERGENiHBG014606.
InParanoidiP25286.
KOiK02154.
PhylomeDBiP25286.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform I (identifier: P25286-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGELFRSEEM TLAQLFLQSE AAYCCVSELE ELGKVQFRDL NPDVNVFQRK
60 70 80 90 100
FVNEVRRCEE MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN
110 120 130 140 150
FEKIENELKE INTNQEALKR NFLELTELKF ILRKTQQFFD EMADPDLLEE
160 170 180 190 200
SSSLLEPNEM GRGAPLRLGF VAGVINRERI PTFERMLWRV CRGNVFLRQA
210 220 230 240 250
EIENPLEDPV TGDYVHKSVF IIFFQGDQLK NRVKKICEGF RASLYPCPET
260 270 280 290 300
PQERKEMASG VNTRIDDLQM VLNQTEDHRQ RVLQAAAKNI RVWFIKVRKM
310 320 330 340 350
KAIYHTLNLC NIDVTQKCLI AEVWCPVTDL DSIQFALRRG TEHSGSTVPS
360 370 380 390 400
ILNRMQTNQT PPTYNKTNKF THGFQNIVDA YGIGTYREIN PAPYTVITFP
410 420 430 440 450
FLFAVMFGDF GHGILMTLFA VWMVLRESRI LSQKNENEMF SMVFSGRYII
460 470 480 490 500
LLMGLFSIYT GLIYNDCFSK SLNIFGSSWS VRPMFTIGNW TEETLLGSSV
510 520 530 540 550
LQLNPAIPGV FGGPYPFGID PIWNIATNKL TFLNSFKMKM SVILGIIHML
560 570 580 590 600
FGVSLSLFNH IYFKKPLNIY FGFIPEIIFM SSLFGYLVIL IFYKWTAYDA
610 620 630 640 650
HSSRNAPSLL IHFINMFLFS YPESGNAMLY SGQKGIQCFL IVVAMLCVPW
660 670 680 690 700
MLLFKPLILR HQYLRKKHLG TLNFGGIRVG NGPTEEDAEI IQHDQLSTHS
710 720 730 740 750
EDAEEPTEDE VFDFGDTMVH QAIHTIEYCL GCISNTASYL RLWALSLAHA
760 770 780 790 800
QLSEVLWTMV IHIGLHVRSL AGGLGLFFIF AAFATLTVAI LLIMEGLSAF
810 820 830
LHALRLHWVE FQNKFYTGTG FKFLPFSFEH IREGKFDE
Length:838
Mass (Da):96,328
Last modified:May 1, 1992 - v1
Checksum:iD25FAD918638D7EE
GO
Isoform II (identifier: P25286-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     706-711: Missing.

Show »
Length:832
Mass (Da):95,657
Checksum:iAAB3018F17D2DDCF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei706 – 7116Missing in isoform II. 1 PublicationVSP_000344

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58758 mRNA. Translation: AAA41962.1.
PIRiB38656.
RefSeqiNP_113792.2. NM_031604.2.
UniGeneiRn.6015.

Genome annotation databases

GeneIDi29757.
KEGGirno:29757.
UCSCiRGD:68405. rat. [P25286-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58758 mRNA. Translation: AAA41962.1.
PIRiB38656.
RefSeqiNP_113792.2. NM_031604.2.
UniGeneiRn.6015.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248368. 2 interactions.
IntActiP25286. 1 interaction.
STRINGi10116.ENSRNOP00000052113.

PTM databases

iPTMnetiP25286.
PhosphoSiteiP25286.

Proteomic databases

PaxDbiP25286.
PRIDEiP25286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29757.
KEGGirno:29757.
UCSCiRGD:68405. rat. [P25286-1]

Organism-specific databases

CTDi535.
RGDi68405. Atp6v0a1.

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
HOVERGENiHBG014606.
InParanoidiP25286.
KOiK02154.
PhylomeDBiP25286.

Miscellaneous databases

PROiP25286.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Structure of the 116-kDa polypeptide of the clathrin-coated vesicle/synaptic vesicle proton pump."
    Perin M.S., Fried V.A., Stone D.K., Xie X.-S., Suedhof T.C.
    J. Biol. Chem. 266:3877-3881(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS I AND II).
    Tissue: Brain.

Entry informationi

Entry nameiVPP1_RAT
AccessioniPrimary (citable) accession number: P25286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.