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Protein

Aminomethyltransferase, mitochondrial

Gene

AMT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine.By similarity

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei226 – 2261SubstrateBy similarity
Binding sitei255 – 2551SubstrateBy similarity
Binding sitei393 – 3931SubstrateBy similarity

GO - Molecular functioni

  • aminomethyltransferase activity Source: UniProtKB-EC
  • lipoic acid binding Source: AgBase
  • transaminase activity Source: UniProtKB-KW

GO - Biological processi

  • glycine catabolic process Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

ReactomeiR-BTA-6783984. Glycine degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminomethyltransferase, mitochondrialCurated (EC:2.1.2.10By similarity)
Alternative name(s):
Glycine cleavage system T protein1 Publication
Short name:
GCVT
Gene namesi
Name:AMTBy similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 22

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

  • mitochondrial inner membrane Source: AgBase
  • mitochondrion Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222Mitochondrion1 PublicationAdd
BLAST
Chaini23 – 397375Aminomethyltransferase, mitochondrialPRO_0000010753Add
BLAST

Proteomic databases

PaxDbiP25285.
PRIDEiP25285.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002992.

Structurei

3D structure databases

ProteinModelPortaliP25285.
SMRiP25285. Positions 26-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2770. Eukaryota.
COG0404. LUCA.
GeneTreeiENSGT00530000063120.
HOGENOMiHOG000239380.
HOVERGENiHBG005822.
InParanoidiP25285.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG7KM5SQ.
TreeFamiTF313026.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSRLSSRLQ ALASAPCRSL SCAQDVLHRT PLYDFHLAHG GKMVAFAGWS
60 70 80 90 100
LPVQYRDSHV NSHLHTRQHC SLFDVSHMLQ TKIFGCDRVK LMESLVVGDI
110 120 130 140 150
AELKPNQGTL SLFTNEAGGI LDDLIVTSAS EGHLYVVSNA GCREKDLTLM
160 170 180 190 200
QDKVRELQNK GSDVALEVMD NALLALQGPT AAQVLQAGVA DDLRKLPFMT
210 220 230 240 250
SAVMEVFGVS GCRVTRCGYT GEDGVEISVP AAEAVHLAAA LLKNPEVKLA
260 270 280 290 300
GLAARDSLRL EAGLCLYGND IDEHTTPVEG SLSWTLGKRR RAAMDFPGAS
310 320 330 340 350
VIVPQLKSKA QRRRVGLMCD GAPVRAQSPI LSPEGTVIGA VTSGCPSPCL
360 370 380 390
KKNVAMGYVP YEYSRPGTPL LVEVRRKQQP AVVSKMPFVS TNYYILK
Length:397
Mass (Da):42,869
Last modified:May 1, 1992 - v1
Checksum:i3FE5D598B78AB6FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59799 mRNA. Translation: AAA30786.1.
PIRiA23707.
RefSeqiNP_803451.1. NM_177485.2.
UniGeneiBt.7177.

Genome annotation databases

EnsembliENSBTAT00000002992; ENSBTAP00000002992; ENSBTAG00000002321.
GeneIDi280719.
KEGGibta:280719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59799 mRNA. Translation: AAA30786.1.
PIRiA23707.
RefSeqiNP_803451.1. NM_177485.2.
UniGeneiBt.7177.

3D structure databases

ProteinModelPortaliP25285.
SMRiP25285. Positions 26-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002992.

Proteomic databases

PaxDbiP25285.
PRIDEiP25285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002992; ENSBTAP00000002992; ENSBTAG00000002321.
GeneIDi280719.
KEGGibta:280719.

Organism-specific databases

CTDi275.

Phylogenomic databases

eggNOGiKOG2770. Eukaryota.
COG0404. LUCA.
GeneTreeiENSGT00530000063120.
HOGENOMiHOG000239380.
HOVERGENiHBG005822.
InParanoidiP25285.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG7KM5SQ.
TreeFamiTF313026.

Enzyme and pathway databases

ReactomeiR-BTA-6783984. Glycine degradation.

Miscellaneous databases

NextBioi20804898.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Isolation and sequence determination of cDNA encoding T-protein of the glycine cleavage system."
    Okamura-Ikeda K., Fujiwara K., Yamamoto M., Hiraga K., Motokawa Y.
    J. Biol. Chem. 266:4917-4921(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-55.
    Tissue: Liver.

Entry informationi

Entry nameiGCST_BOVIN
AccessioniPrimary (citable) accession number: P25285
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.