Reviewed, UniProtKB/Swiss-Prot P25285 (GCST_BOVIN)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase, mitochondrial
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein
      Short name=GCVT

Gene names

Name:

AMT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Aminotransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycine catabolic process Ref.1 Traceable author statement. Source: AgBase
Cellular component	mitochondrial inner membrane Traceable author statement. Source: AgBase
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC lipoic acid binding Inferred from direct assay. Source: AgBase transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Ref.1
	Chain	23 – 397	375	Aminomethyltransferase, mitochondrial		PRO_0000010753

Sequences

	Sequence		Length	Mass (Da)
	P25285-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 3FE5D598B78AB6FD	FASTA	397	42,869
	10 20 30 40 50 60 MVSRLSSRLQ ALASAPCRSL SCAQDVLHRT PLYDFHLAHG GKMVAFAGWS LPVQYRDSHV 70 80 90 100 110 120 NSHLHTRQHC SLFDVSHMLQ TKIFGCDRVK LMESLVVGDI AELKPNQGTL SLFTNEAGGI 130 140 150 160 170 180 LDDLIVTSAS EGHLYVVSNA GCREKDLTLM QDKVRELQNK GSDVALEVMD NALLALQGPT 190 200 210 220 230 240 AAQVLQAGVA DDLRKLPFMT SAVMEVFGVS GCRVTRCGYT GEDGVEISVP AAEAVHLAAA 250 260 270 280 290 300 LLKNPEVKLA GLAARDSLRL EAGLCLYGND IDEHTTPVEG SLSWTLGKRR RAAMDFPGAS 310 320 330 340 350 360 VIVPQLKSKA QRRRVGLMCD GAPVRAQSPI LSPEGTVIGA VTSGCPSPCL KKNVAMGYVP 370 380 390 YEYSRPGTPL LVEVRRKQQP AVVSKMPFVS TNYYILK

References

[1]

"Isolation and sequence determination of cDNA encoding T-protein of the glycine cleavage system." Okamura-Ikeda K., Fujiwara K., Yamamoto M., Hiraga K., Motokawa Y. J. Biol. Chem. 266:4917-4921(1991) [PubMed: 2002038] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-55. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M59799 mRNA. Translation: AAA30786.1.
IPI	IPI00700367.
PIR	A23707.
RefSeq	NP_803451.1.
UniGene	Bt.7177
3D structure databases
SMR	P25285. Positions 26-396.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000002321. Bos taurus. [Contig view]
GeneID	280719.
KEGG	bta:280719.
Phylogenomic databases
HOVERGEN	P25285.
Enzyme and pathway databases
BRENDA	2.1.2.10. 251.
Family and domain databases
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BOVIN

Accession

Primary (citable) accession number: P25285

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)