Reviewed, UniProtKB/Swiss-Prot P25283 (MTG2_HAEGA)

Names and origin

Protein names

Recommended name:
    Modification methylase HgaIB
      Short name=M.HgaIB
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase HgaIB
    M.HgaI-2

Gene names

Name:

hgaIBM

Organism

Haemophilus gallinarum

Taxonomic identifier

728 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Avibacterium

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the strands carrying the 5'-GACGC-3' sequence, causes specific methylation on C-3, and protects the DNA from cleavage by the HgaI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Miscellaneous	The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Modification methylase HgaIB		PRO_0000087880
Sites
	Active site	81	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25283-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 0373563207685A91	FASTA	358	40,690
	10 20 30 40 50 60 MKINAMSLFS SAGIGELDLH KGNLNFVVAN ELLKKRADTY QFFYPETKMF QGDISDEKLK 70 80 90 100 110 120 REILLSAQQN NVKFLLATPP CQGLSSVGKN KHQDHFIKDN RNFLIFEVFE FIDVLNLDFI 130 140 150 160 170 180 LIENVPRFIE MYFPYNGQLL LLEEILKIKY ASKYQIDIVI LNAKDYGICQ SRPRAIIKMY 190 200 210 220 230 240 KYGITWKLPT IQAEISLQRA IGHLPPLEPG EVSSIKWHSA PNVKPSIIEA IRHTKPGTSA 250 260 270 280 290 300 ISNPIFYPKK DNGERIKGFH NTYKRMEWDK PAPARTTYSG SISSHNNIHP GRLQLDGTYS 310 320 330 340 350 DPRVLSLLET FIVSSIDENI EFPPGSSETY IRTIIGEAIP PKLLSAICFP DGENINVK

References

[1]

"The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands." Sugisaki H., Yamamoto K., Takanami M. J. Biol. Chem. 266:13952-13957(1991) [PubMed: 1856224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 3438.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D17388 Genomic DNA. Translation: BAA04207.1. D90363 Genomic DNA. Translation: BAA14378.1.
PIR	A39467.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3651. M2.HgaI.
Enzyme and pathway databases
BRENDA	2.1.1.37. 2318.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTG2_HAEGA

Accession

Primary (citable) accession number: P25283

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)