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P25283

- MTG2_AVIPA

UniProt

P25283 - MTG2_AVIPA

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Protein

Modification methylase HgaIB

Gene

hgaIBM

Organism
Avibacterium paragallinarum (Haemophilus gallinarum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the strands carrying the 5'-GACGC-3' sequence, causes specific methylation on C-3, and protects the DNA from cleavage by the HgaI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgaIB (EC:2.1.1.37)
Short name:
M.HgaIB
Alternative name(s):
Cytosine-specific methyltransferase HgaIB
M.HgaI-2
Gene namesi
Name:hgaIBM
OrganismiAvibacterium paragallinarum (Haemophilus gallinarum)
Taxonomic identifieri728 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Modification methylase HgaIBPRO_0000087880Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP25283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 358356SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25283-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKINAMSLFS SAGIGELDLH KGNLNFVVAN ELLKKRADTY QFFYPETKMF
60 70 80 90 100
QGDISDEKLK REILLSAQQN NVKFLLATPP CQGLSSVGKN KHQDHFIKDN
110 120 130 140 150
RNFLIFEVFE FIDVLNLDFI LIENVPRFIE MYFPYNGQLL LLEEILKIKY
160 170 180 190 200
ASKYQIDIVI LNAKDYGICQ SRPRAIIKMY KYGITWKLPT IQAEISLQRA
210 220 230 240 250
IGHLPPLEPG EVSSIKWHSA PNVKPSIIEA IRHTKPGTSA ISNPIFYPKK
260 270 280 290 300
DNGERIKGFH NTYKRMEWDK PAPARTTYSG SISSHNNIHP GRLQLDGTYS
310 320 330 340 350
DPRVLSLLET FIVSSIDENI EFPPGSSETY IRTIIGEAIP PKLLSAICFP

DGENINVK
Length:358
Mass (Da):40,690
Last modified:May 1, 1992 - v1
Checksum:i0373563207685A91
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17388 Genomic DNA. Translation: BAA04207.1.
D90363 Genomic DNA. Translation: BAA14378.1.
PIRiA39467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17388 Genomic DNA. Translation: BAA04207.1 .
D90363 Genomic DNA. Translation: BAA14378.1 .
PIRi A39467.

3D structure databases

ProteinModelPortali P25283.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands."
    Sugisaki H., Yamamoto K., Takanami M.
    J. Biol. Chem. 266:13952-13957(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCTC 3438.

Entry informationi

Entry nameiMTG2_AVIPA
AccessioniPrimary (citable) accession number: P25283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 1, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3