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P25283 (MTG2_AVIPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HgaIB

Short name=M.HgaIB
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase HgaIB
M.HgaI-2
Gene names
Name:hgaIBM
OrganismAvibacterium paragallinarum (Haemophilus gallinarum)
Taxonomic identifier728 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the strands carrying the 5'-GACGC-3' sequence, causes specific methylation on C-3, and protects the DNA from cleavage by the HgaI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Miscellaneous

The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Modification methylase HgaIB
PRO_0000087880

Regions

Domain3 – 358356SAM-dependent MTase C5-type

Sites

Active site811 By similarity

Sequences

Sequence LengthMass (Da)Tools
P25283 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 0373563207685A91

FASTA35840,690
        10         20         30         40         50         60 
MKINAMSLFS SAGIGELDLH KGNLNFVVAN ELLKKRADTY QFFYPETKMF QGDISDEKLK 

        70         80         90        100        110        120 
REILLSAQQN NVKFLLATPP CQGLSSVGKN KHQDHFIKDN RNFLIFEVFE FIDVLNLDFI 

       130        140        150        160        170        180 
LIENVPRFIE MYFPYNGQLL LLEEILKIKY ASKYQIDIVI LNAKDYGICQ SRPRAIIKMY 

       190        200        210        220        230        240 
KYGITWKLPT IQAEISLQRA IGHLPPLEPG EVSSIKWHSA PNVKPSIIEA IRHTKPGTSA 

       250        260        270        280        290        300 
ISNPIFYPKK DNGERIKGFH NTYKRMEWDK PAPARTTYSG SISSHNNIHP GRLQLDGTYS 

       310        320        330        340        350 
DPRVLSLLET FIVSSIDENI EFPPGSSETY IRTIIGEAIP PKLLSAICFP DGENINVK 

« Hide

References

[1]"The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands."
Sugisaki H., Yamamoto K., Takanami M.
J. Biol. Chem. 266:13952-13957(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCTC 3438.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17388 Genomic DNA. Translation: BAA04207.1.
D90363 Genomic DNA. Translation: BAA14378.1.
PIRA39467.

3D structure databases

ProteinModelPortalP25283.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTG2_AVIPA
AccessionPrimary (citable) accession number: P25283
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries