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Protein

Modification methylase HgaIB

Gene

hgaIBM

Organism
Avibacterium paragallinarum (Haemophilus gallinarum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the strands carrying the 5'-GACGC-3' sequence, causes specific methylation on C-3, and protects the DNA from cleavage by the HgaI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgaIB (EC:2.1.1.37)
Short name:
M.HgaIB
Alternative name(s):
Cytosine-specific methyltransferase HgaIB
M.HgaI-2
Gene namesi
Name:hgaIBM
OrganismiAvibacterium paragallinarum (Haemophilus gallinarum)
Taxonomic identifieri728 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Modification methylase HgaIBPRO_0000087880Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP25283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 358356SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKINAMSLFS SAGIGELDLH KGNLNFVVAN ELLKKRADTY QFFYPETKMF
60 70 80 90 100
QGDISDEKLK REILLSAQQN NVKFLLATPP CQGLSSVGKN KHQDHFIKDN
110 120 130 140 150
RNFLIFEVFE FIDVLNLDFI LIENVPRFIE MYFPYNGQLL LLEEILKIKY
160 170 180 190 200
ASKYQIDIVI LNAKDYGICQ SRPRAIIKMY KYGITWKLPT IQAEISLQRA
210 220 230 240 250
IGHLPPLEPG EVSSIKWHSA PNVKPSIIEA IRHTKPGTSA ISNPIFYPKK
260 270 280 290 300
DNGERIKGFH NTYKRMEWDK PAPARTTYSG SISSHNNIHP GRLQLDGTYS
310 320 330 340 350
DPRVLSLLET FIVSSIDENI EFPPGSSETY IRTIIGEAIP PKLLSAICFP

DGENINVK
Length:358
Mass (Da):40,690
Last modified:April 30, 1992 - v1
Checksum:i0373563207685A91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17388 Genomic DNA. Translation: BAA04207.1.
D90363 Genomic DNA. Translation: BAA14378.1.
PIRiA39467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17388 Genomic DNA. Translation: BAA04207.1.
D90363 Genomic DNA. Translation: BAA14378.1.
PIRiA39467.

3D structure databases

ProteinModelPortaliP25283.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands."
    Sugisaki H., Yamamoto K., Takanami M.
    J. Biol. Chem. 266:13952-13957(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCTC 3438.

Entry informationi

Entry nameiMTG2_AVIPA
AccessioniPrimary (citable) accession number: P25283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1992
Last sequence update: April 30, 1992
Last modified: January 6, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.