Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P25283

- MTG2_AVIPA

UniProt

P25283 - MTG2_AVIPA

Protein

Modification methylase HgaIB

Gene

hgaIBM

Organism
Avibacterium paragallinarum (Haemophilus gallinarum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the strands carrying the 5'-GACGC-3' sequence, causes specific methylation on C-3, and protects the DNA from cleavage by the HgaI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei81 – 811PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HgaIB (EC:2.1.1.37)
    Short name:
    M.HgaIB
    Alternative name(s):
    Cytosine-specific methyltransferase HgaIB
    M.HgaI-2
    Gene namesi
    Name:hgaIBM
    OrganismiAvibacterium paragallinarum (Haemophilus gallinarum)
    Taxonomic identifieri728 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Modification methylase HgaIBPRO_0000087880Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP25283.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 358356SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25283-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKINAMSLFS SAGIGELDLH KGNLNFVVAN ELLKKRADTY QFFYPETKMF    50
    QGDISDEKLK REILLSAQQN NVKFLLATPP CQGLSSVGKN KHQDHFIKDN 100
    RNFLIFEVFE FIDVLNLDFI LIENVPRFIE MYFPYNGQLL LLEEILKIKY 150
    ASKYQIDIVI LNAKDYGICQ SRPRAIIKMY KYGITWKLPT IQAEISLQRA 200
    IGHLPPLEPG EVSSIKWHSA PNVKPSIIEA IRHTKPGTSA ISNPIFYPKK 250
    DNGERIKGFH NTYKRMEWDK PAPARTTYSG SISSHNNIHP GRLQLDGTYS 300
    DPRVLSLLET FIVSSIDENI EFPPGSSETY IRTIIGEAIP PKLLSAICFP 350
    DGENINVK 358
    Length:358
    Mass (Da):40,690
    Last modified:May 1, 1992 - v1
    Checksum:i0373563207685A91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17388 Genomic DNA. Translation: BAA04207.1.
    D90363 Genomic DNA. Translation: BAA14378.1.
    PIRiA39467.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17388 Genomic DNA. Translation: BAA04207.1 .
    D90363 Genomic DNA. Translation: BAA14378.1 .
    PIRi A39467.

    3D structure databases

    ProteinModelPortali P25283.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands."
      Sugisaki H., Yamamoto K., Takanami M.
      J. Biol. Chem. 266:13952-13957(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NCTC 3438.

    Entry informationi

    Entry nameiMTG2_AVIPA
    AccessioniPrimary (citable) accession number: P25283
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3