Reviewed, UniProtKB/Swiss-Prot P25282 (MTG1_HAEGA)

Names and origin

Protein names

Recommended name:
    Modification methylase HgaIA
      Short name=M.HgaIA
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase HgaIA
    M.HgaI-1

Gene names

Name:

hgaIAM

Organism

Haemophilus gallinarum

Taxonomic identifier

728 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Avibacterium

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the strands carrying the 5'-GCGTC-3' sequence, causes specific methylation on C-2, and protects the DNA from cleavage by the HgaI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Miscellaneous	The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Modification methylase HgaIA		PRO_0000087879
Sites
	Active site	83	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25282-1 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 9F3E10D620AF3154	FASTA	357	40,863
	10 20 30 40 50 60 MKKNIMGLSL FSSAGIGEYF LSRVGIDIIV ANELIKKRAD LYQKIYPNHK MVIGDIRDQR 70 80 90 100 110 120 IFNKVLNIAL TNQVDFLIAS PPCQGMSVAG KNRDVSNMAN DNRNYLIMYV IAMIKKLKPA 130 140 150 160 170 180 YILIENVPFL LKLELYIDNK LTPIKNILED EFGSEYHIHF DILDAADYGT PQRRKRAIIR 190 200 210 220 230 240 LNKKGTIWNL PLKQNIVSVE QAIGNLPSIE SGKHSGLKWH YGRGHTEQQI EWMKHTPTGK 250 260 270 280 290 300 SAFENLVHYP RKANGEKVKG YHSSYRRIRW DEPAPTITIR NDAISSQRNV HPGRPLLDGT 310 320 330 340 350 YSDARVLSVL ELMRLTGLPD NWEIPDDTPE ILIRQIIGEC IPPLLIENIT REIFNEN

References

[1]	"The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands." Sugisaki H., Yamamoto K., Takanami M. J. Biol. Chem. 266:13952-13957(1991) [PubMed: 1856224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 3438.
[2]	"Nucleotide sequence of the gene of HgaI restriction endonuclease." Sugisaki H. Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 3438.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90363 Genomic DNA. Translation: BAA14377.1. Different initiation. D17388 Genomic DNA. Translation: BAA04206.1. Different initiation.
PIR	B39467.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3650. M1.HgaI.
Enzyme and pathway databases
BRENDA	2.1.1.37. 2318.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTG1_HAEGA

Accession

Primary (citable) accession number: P25282

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1994
Last modified:	February 9, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)