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P25282

- MTG1_AVIPA

UniProt

P25282 - MTG1_AVIPA

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Protein

Modification methylase HgaIA

Gene
hgaIAM
Organism
Avibacterium paragallinarum (Haemophilus gallinarum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the strands carrying the 5'-GCGTC-3' sequence, causes specific methylation on C-2, and protects the DNA from cleavage by the HgaI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgaIA (EC:2.1.1.37)
Short name:
M.HgaIA
Alternative name(s):
Cytosine-specific methyltransferase HgaIA
M.HgaI-1
Gene namesi
Name:hgaIAM
OrganismiAvibacterium paragallinarum (Haemophilus gallinarum)
Taxonomic identifieri728 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Modification methylase HgaIAPRO_0000087879Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP25282.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 357353SAM-dependent MTase C5-typeAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25282-1 [UniParc]FASTAAdd to Basket

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MKKNIMGLSL FSSAGIGEYF LSRVGIDIIV ANELIKKRAD LYQKIYPNHK    50
MVIGDIRDQR IFNKVLNIAL TNQVDFLIAS PPCQGMSVAG KNRDVSNMAN 100
DNRNYLIMYV IAMIKKLKPA YILIENVPFL LKLELYIDNK LTPIKNILED 150
EFGSEYHIHF DILDAADYGT PQRRKRAIIR LNKKGTIWNL PLKQNIVSVE 200
QAIGNLPSIE SGKHSGLKWH YGRGHTEQQI EWMKHTPTGK SAFENLVHYP 250
RKANGEKVKG YHSSYRRIRW DEPAPTITIR NDAISSQRNV HPGRPLLDGT 300
YSDARVLSVL ELMRLTGLPD NWEIPDDTPE ILIRQIIGEC IPPLLIENIT 350
REIFNEN 357
Length:357
Mass (Da):40,863
Last modified:February 1, 1994 - v2
Checksum:i9F3E10D620AF3154
GO

Sequence cautioni

The sequence BAA04206.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAA14377.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90363 Genomic DNA. Translation: BAA14377.1. Different initiation.
D17388 Genomic DNA. Translation: BAA04206.1. Different initiation.
PIRiB39467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90363 Genomic DNA. Translation: BAA14377.1 . Different initiation.
D17388 Genomic DNA. Translation: BAA04206.1 . Different initiation.
PIRi B39467.

3D structure databases

ProteinModelPortali P25282.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands."
    Sugisaki H., Yamamoto K., Takanami M.
    J. Biol. Chem. 266:13952-13957(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCTC 3438.
  2. "Nucleotide sequence of the gene of HgaI restriction endonuclease."
    Sugisaki H.
    Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCTC 3438.

Entry informationi

Entry nameiMTG1_AVIPA
AccessioniPrimary (citable) accession number: P25282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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