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Protein

Modification methylase HgaIA

Gene

hgaIAM

Organism
Avibacterium paragallinarum (Haemophilus gallinarum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the strands carrying the 5'-GCGTC-3' sequence, causes specific methylation on C-2, and protects the DNA from cleavage by the HgaI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei83PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgaIA (EC:2.1.1.37)
Short name:
M.HgaIA
Alternative name(s):
Cytosine-specific methyltransferase HgaIA
M.HgaI-1
Gene namesi
Name:hgaIAM
OrganismiAvibacterium paragallinarum (Haemophilus gallinarum)
Taxonomic identifieri728 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878791 – 357Modification methylase HgaIAAdd BLAST357

Structurei

3D structure databases

ProteinModelPortaliP25282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 357SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST353

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

P25282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNIMGLSL FSSAGIGEYF LSRVGIDIIV ANELIKKRAD LYQKIYPNHK
60 70 80 90 100
MVIGDIRDQR IFNKVLNIAL TNQVDFLIAS PPCQGMSVAG KNRDVSNMAN
110 120 130 140 150
DNRNYLIMYV IAMIKKLKPA YILIENVPFL LKLELYIDNK LTPIKNILED
160 170 180 190 200
EFGSEYHIHF DILDAADYGT PQRRKRAIIR LNKKGTIWNL PLKQNIVSVE
210 220 230 240 250
QAIGNLPSIE SGKHSGLKWH YGRGHTEQQI EWMKHTPTGK SAFENLVHYP
260 270 280 290 300
RKANGEKVKG YHSSYRRIRW DEPAPTITIR NDAISSQRNV HPGRPLLDGT
310 320 330 340 350
YSDARVLSVL ELMRLTGLPD NWEIPDDTPE ILIRQIIGEC IPPLLIENIT

REIFNEN
Length:357
Mass (Da):40,863
Last modified:February 1, 1994 - v2
Checksum:i9F3E10D620AF3154
GO

Sequence cautioni

The sequence BAA04206 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA14377 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90363 Genomic DNA. Translation: BAA14377.1. Different initiation.
D17388 Genomic DNA. Translation: BAA04206.1. Different initiation.
PIRiB39467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90363 Genomic DNA. Translation: BAA14377.1. Different initiation.
D17388 Genomic DNA. Translation: BAA04206.1. Different initiation.
PIRiB39467.

3D structure databases

ProteinModelPortaliP25282.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTG1_AVIPA
AccessioniPrimary (citable) accession number: P25282
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: February 15, 2017
This is version 77 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.