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P25282 (MTG1_AVIPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HgaIA

Short name=M.HgaIA
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase HgaIA
M.HgaI-1
Gene names
Name:hgaIAM
OrganismAvibacterium paragallinarum (Haemophilus gallinarum)
Taxonomic identifier728 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the strands carrying the 5'-GCGTC-3' sequence, causes specific methylation on C-2, and protects the DNA from cleavage by the HgaI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Miscellaneous

The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence caution

The sequence BAA04206.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA14377.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Modification methylase HgaIA
PRO_0000087879

Regions

Domain5 – 357353SAM-dependent MTase C5-type

Sites

Active site831 By similarity

Sequences

Sequence LengthMass (Da)Tools
P25282 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 9F3E10D620AF3154

FASTA35740,863
        10         20         30         40         50         60 
MKKNIMGLSL FSSAGIGEYF LSRVGIDIIV ANELIKKRAD LYQKIYPNHK MVIGDIRDQR 

        70         80         90        100        110        120 
IFNKVLNIAL TNQVDFLIAS PPCQGMSVAG KNRDVSNMAN DNRNYLIMYV IAMIKKLKPA 

       130        140        150        160        170        180 
YILIENVPFL LKLELYIDNK LTPIKNILED EFGSEYHIHF DILDAADYGT PQRRKRAIIR 

       190        200        210        220        230        240 
LNKKGTIWNL PLKQNIVSVE QAIGNLPSIE SGKHSGLKWH YGRGHTEQQI EWMKHTPTGK 

       250        260        270        280        290        300 
SAFENLVHYP RKANGEKVKG YHSSYRRIRW DEPAPTITIR NDAISSQRNV HPGRPLLDGT 

       310        320        330        340        350 
YSDARVLSVL ELMRLTGLPD NWEIPDDTPE ILIRQIIGEC IPPLLIENIT REIFNEN 

« Hide

References

[1]"The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands."
Sugisaki H., Yamamoto K., Takanami M.
J. Biol. Chem. 266:13952-13957(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCTC 3438.
[2]"Nucleotide sequence of the gene of HgaI restriction endonuclease."
Sugisaki H.
Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCTC 3438.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90363 Genomic DNA. Translation: BAA14377.1. Different initiation.
D17388 Genomic DNA. Translation: BAA04206.1. Different initiation.
PIRB39467.

3D structure databases

ProteinModelPortalP25282.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTG1_AVIPA
AccessionPrimary (citable) accession number: P25282
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries