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Protein

Tryptophan synthase beta chain 2, chloroplastic

Gene

TSB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (AXX17_At5g53930), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (AXX17_At5g28490), Tryptophan synthase (AXX17_At4g31220), Tryptophan synthase, Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 2, chloroplastic (TSB2), Tryptophan synthase (AXX17_At4g31220)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • tryptophan synthase activity Source: TAIR

GO - Biological processi

  • tryptophan biosynthetic process Source: TAIR

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT4G27070-MONOMER
UniPathwayiUPA00035; UER00044

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain 2, chloroplastic (EC:4.2.1.20)
Gene namesi
Name:TSB2
Ordered Locus Names:At4g27070
ORF Names:T24A18.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G27070
TAIRilocus:2005543 AT4G27070

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 51ChloroplastCombined sourcesAdd BLAST51
ChainiPRO_000003578452 – 475Tryptophan synthase beta chain 2, chloroplasticAdd BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52N-acetylthreonineCombined sources1
Modified residuei170N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP25269
PRIDEiP25269

PTM databases

iPTMnetiP25269

Expressioni

Gene expression databases

ExpressionAtlasiP25269 baseline and differential
GenevisibleiP25269 AT

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.

Protein-protein interaction databases

BioGridi14102, 1 interactor
STRINGi3702.AT4G27070.1

Structurei

3D structure databases

ProteinModelPortaliP25269
SMRiP25269
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1395 Eukaryota
COG0133 LUCA
HOGENOMiHOG000161710
InParanoidiP25269
KOiK01696
OMAiDPFPRMV
OrthoDBiEOG093609DQ
PhylomeDBiP25269

Family and domain databases

CDDicd06446 Trp-synth_B, 1 hit
HAMAPiMF_00133 Trp_synth_beta, 1 hit
InterProiView protein in InterPro
IPR001926 PLP-dep
IPR006653 Trp_synth_b_CS
IPR006654 Trp_synth_beta
IPR023026 Trp_synth_beta/beta-like
IPR036052 Trypto_synt_PLP_dependent
PANTHERiPTHR42882 PTHR42882, 1 hit
PfamiView protein in Pfam
PF00291 PALP, 1 hit
PIRSFiPIRSF001413 Trp_syn_beta, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR00263 trpB, 1 hit
PROSITEiView protein in PROSITE
PS00168 TRP_SYNTHASE_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATASTAATF RPSSVSASSE LTHLRSPSKL PKFTPLPSAR SRSSSSFSVS
60 70 80 90 100
CTIAKDPAVV MADSEKIKAA GSDPTMWQRP DSFGRFGKFG GKYVPETLMH
110 120 130 140 150
ALSELETAFY SLATDEDFQR ELAEILKDYV GRESPLYFAE RLTEHYRREN
160 170 180 190 200
GEGPLIYLKR EDLNHTGAHK INNAVAQALL AKRLGKKRII AETGAGQHGV
210 220 230 240 250
ATATVCARFG LQCIIYMGAQ DMERQALNVF RMRLLGAEVR GVHSGTATLK
260 270 280 290 300
DATSEAIRDW VTNVETTHYI LGSVAGPHPY PMMVRDFHAV IGKETRKQAM
310 320 330 340 350
EKWGGKPDVL VACVGGGSNA MGLFHEFVDD TEVRMIGVEA AGFGLDSGKH
360 370 380 390 400
AATLTKGDVG VLHGAMSYLL QDDDGQIIEP HSISAGLDYP GVGPEHSFLK
410 420 430 440 450
DVGRAEYFSV TDEEALEAFK RVSRLEGIIP ALETSHALAH LEKLCPTLPD
460 470
GARVVLNFSG RGDKDVQTAI KYLEV
Length:475
Mass (Da):51,601
Last modified:November 1, 1995 - v2
Checksum:i1222EDA3B4CDBEEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81620 Genomic DNA Translation: AAA32879.1
AL035680 Genomic DNA Translation: CAB38837.1
AL161566 Genomic DNA Translation: CAB79562.1
CP002687 Genomic DNA Translation: AEE85296.1
BT003144 mRNA Translation: AAO24576.1
AY084334 mRNA Translation: AAM60917.1
PIRiJQ1073
T06037
RefSeqiNP_194437.1, NM_118841.5
UniGeneiAt.327

Genome annotation databases

EnsemblPlantsiAT4G27070.1; AT4G27070.1; AT4G27070
GeneIDi828815
GrameneiAT4G27070.1; AT4G27070.1; AT4G27070
KEGGiath:AT4G27070

Similar proteinsi

Entry informationi

Entry nameiTRBP2_ARATH
AccessioniPrimary (citable) accession number: P25269
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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