Reviewed, UniProtKB/Swiss-Prot P25267 (MTG1_HERAU)

Names and origin

Protein names

Recommended name:
    Modification methylase HgiGI
      Short name=M.HgiGI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase HgiGI

Gene names

Name:

hgiGIM

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	126 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiEI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›126	›126	Modification methylase HgiGI		PRO_0000087891
Sites
	Active site	75	1	By similarity
Experimental info
	Non-terminal residue	126	1

Sequences

	Sequence		Length	Mass (Da)
	P25267-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: FF3695B03BC02399	FASTA	126	13,871
	10 20 30 40 50 60 MKTIDLFAGC GGMSLGFMQA GFEIVAAVDN WRPAITTYQQ NFIHPIHELD LAEVDEAISL 70 80 90 100 110 120 IKTYSPELII GGPPCQDFSS AGKRDEGLGR ANLTLDFVKI VLAIQPAWVI MENVERARLS KIHQQA

References

[1]	Duesterhoeft A., Kroeger M. Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPA1.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed: 7607523] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55143 Genomic DNA. Translation: CAA38947.1.
PIR	S21955.
3D structure databases
SMR	P25267. Positions 1-117.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.37. 291850.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTG1_HERAU

Accession

Primary (citable) accession number: P25267

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 19, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)