P25266 (MTE1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase HgiEI
Short name=M.HgiEI
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase HgiEI

Gene names

Name:

hgiEIM

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiEI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Modification methylase HgiEI		PRO_0000087890
Sites
	Active site	75	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25266 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: AC27AF38B5BC973F	FASTA	437	49,817
	10 20 30 40 50 60 MQQFRFIDLF AGIGGFRLGL EAVGGVCVAS AEIDQQAIKV YWQNWPTDGV DHNLGDITQI 70 80 90 100 110 120 QQLPAHDVLV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR LVQINQPKAF IFENVKGLVD 130 140 150 160 170 180 PRNRLCLEII LDSFKDLGYS VFYKLLNSFD FGVAQNRDRV FIVGIQQKLD LNGFSFPEYA 190 200 210 220 230 240 ESDQRLYHIL DNLEAPETKL ESIPIQRNLF GERIEVGYNK LTPRGAFNDF FILNDIRNGP 250 260 270 280 290 300 TSIHSWEIYP TTEREKHICM IIMRNRRNSR YGNCDGNPMS YSDIAELVVD LAENELQILV 310 320 330 340 350 360 KKRILRQYPD GKYEFFNRRL SGGIDGTYRI FMPNARFFGT LTARGMHDEI AEINVSGANA 370 380 390 400 410 420 AEYKYNFIQQ VLIPKRYRPI TVSEAARLQG FPSTFKFHSN QSANFRLIGN SVAPPVIVAL 430 GKRLQCVKLF EQELCEV

References

[1]	Erdmann D., Kroeger M. Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPG24.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55142 Genomic DNA. Translation: CAA38944.1.
3D structure databases
ProteinModelPortal	P25266.
ModBase	Search...
Protein family/group databases
REBASE	3419. M.HgiEI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTE1_HERAU

Accession

Primary (citable) accession number: P25266

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program