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P25266 (MTE1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HgiEI

Short name=M.HgiEI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase HgiEI
Gene names
Name:hgiEIM
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiEI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 437437Modification methylase HgiEI
PRO_0000087890

Regions

Domain4 – 431428SAM-dependent MTase C5-type

Sites

Active site751 By similarity

Sequences

Sequence LengthMass (Da)Tools
P25266 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: AC27AF38B5BC973F

FASTA43749,817
        10         20         30         40         50         60 
MQQFRFIDLF AGIGGFRLGL EAVGGVCVAS AEIDQQAIKV YWQNWPTDGV DHNLGDITQI 

        70         80         90        100        110        120 
QQLPAHDVLV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR LVQINQPKAF IFENVKGLVD 

       130        140        150        160        170        180 
PRNRLCLEII LDSFKDLGYS VFYKLLNSFD FGVAQNRDRV FIVGIQQKLD LNGFSFPEYA 

       190        200        210        220        230        240 
ESDQRLYHIL DNLEAPETKL ESIPIQRNLF GERIEVGYNK LTPRGAFNDF FILNDIRNGP 

       250        260        270        280        290        300 
TSIHSWEIYP TTEREKHICM IIMRNRRNSR YGNCDGNPMS YSDIAELVVD LAENELQILV 

       310        320        330        340        350        360 
KKRILRQYPD GKYEFFNRRL SGGIDGTYRI FMPNARFFGT LTARGMHDEI AEINVSGANA 

       370        380        390        400        410        420 
AEYKYNFIQQ VLIPKRYRPI TVSEAARLQG FPSTFKFHSN QSANFRLIGN SVAPPVIVAL 

       430 
GKRLQCVKLF EQELCEV 

« Hide

References

[1]Erdmann D., Kroeger M.
Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPG24.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55142 Genomic DNA. Translation: CAA38944.1.

3D structure databases

ProteinModelPortalP25266.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3419. M.HgiEI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTE1_HERAU
AccessionPrimary (citable) accession number: P25266
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries