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Protein

Modification methylase HgiEI

Gene

hgiEIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiEI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3419. M.HgiEI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgiEI (EC:2.1.1.37)
Short name:
M.HgiEI
Alternative name(s):
Cytosine-specific methyltransferase HgiEI
Gene namesi
Name:hgiEIM
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878901 – 437Modification methylase HgiEIAdd BLAST437

Structurei

3D structure databases

ProteinModelPortaliP25266.
SMRiP25266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 431SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST428

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

P25266-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQFRFIDLF AGIGGFRLGL EAVGGVCVAS AEIDQQAIKV YWQNWPTDGV
60 70 80 90 100
DHNLGDITQI QQLPAHDVLV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR
110 120 130 140 150
LVQINQPKAF IFENVKGLVD PRNRLCLEII LDSFKDLGYS VFYKLLNSFD
160 170 180 190 200
FGVAQNRDRV FIVGIQQKLD LNGFSFPEYA ESDQRLYHIL DNLEAPETKL
210 220 230 240 250
ESIPIQRNLF GERIEVGYNK LTPRGAFNDF FILNDIRNGP TSIHSWEIYP
260 270 280 290 300
TTEREKHICM IIMRNRRNSR YGNCDGNPMS YSDIAELVVD LAENELQILV
310 320 330 340 350
KKRILRQYPD GKYEFFNRRL SGGIDGTYRI FMPNARFFGT LTARGMHDEI
360 370 380 390 400
AEINVSGANA AEYKYNFIQQ VLIPKRYRPI TVSEAARLQG FPSTFKFHSN
410 420 430
QSANFRLIGN SVAPPVIVAL GKRLQCVKLF EQELCEV
Length:437
Mass (Da):49,817
Last modified:May 1, 1992 - v1
Checksum:iAC27AF38B5BC973F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55142 Genomic DNA. Translation: CAA38944.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55142 Genomic DNA. Translation: CAA38944.1.

3D structure databases

ProteinModelPortaliP25266.
SMRiP25266.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3419. M.HgiEI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTE1_HERAU
AccessioniPrimary (citable) accession number: P25266
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 15, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.