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Protein

Modification methylase HgiDII

Gene

hgiDIIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3418. M.HgiDII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgiDII (EC:2.1.1.37)
Short name:
M.HgiDII
Alternative name(s):
Cytosine-specific methyltransferase HgiDII
Gene namesi
Name:hgiDIIM
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Modification methylase HgiDIIPRO_0000087889Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP25265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 344342SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25265-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MVGAVIDLFC GVGGLTHGLI LEGFGVLAGI DNDPSCKYAY EQNNRTRFIE
60 70 80 90 100
KSISEVDGRE LNALYPNNQH KILVGCAPCQ DFSQYTKKSR TGTKWQLLTE
110 120 130 140 150
FSRLIREIEP DIISMENVPE VRTFNRGEVF NNFIQSLEQL GYHVSHSVVH
160 170 180 190 200
CPDYGIPQQR DRLVLFAAKQ GVIKIIPPTH TPENYRTVRD VIGSLATNYS
210 220 230 240 250
GGHWEGDSMH AASRLEDINL RRIQHSVPGG TWADWPEELI AECHKKESGE
260 270 280 290 300
SYGSVYGRME WDKVAPTITT QCNGYGNGRF GHPEQDRAIS LREAALLQTF
310 320 330 340 350
PRSYQFAPEG QLKFKTVSRQ IGNAVPVALG RVIAKSIKRF LEGLHERQRV

RIII
Length:354
Mass (Da):39,853
Last modified:May 1, 1992 - v1
Checksum:iBDA017785D5ADC75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55141 Genomic DNA. Translation: CAA38941.1.
PIRiS21949. JT0594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55141 Genomic DNA. Translation: CAA38941.1.
PIRiS21949. JT0594.

3D structure databases

ProteinModelPortaliP25265.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3418. M.HgiDII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequence and characterization of m5C-methyltransferase-encoding gene, hgiDIIM (GTCGAC), from Herpetosiphon giganteus strain Hpa2."
    Duesterhoeft A., Kroeger M.
    Gene 106:87-92(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPA2.
  2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
    Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
    Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiMTD2_HERAU
AccessioniPrimary (citable) accession number: P25265
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: January 7, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.