Reviewed, UniProtKB/Swiss-Prot P25265 (MTD2_HERAU)

Names and origin

Protein names

Recommended name:
    Modification methylase HgiDII
      Short name=M.HgiDII
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase HgiDII

Gene names

Name:

hgiDIIM

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Modification methylase HgiDII		PRO_0000087889
Sites
	Active site	79	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25265-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: BDA017785D5ADC75	FASTA	354	39,853
	10 20 30 40 50 60 MVGAVIDLFC GVGGLTHGLI LEGFGVLAGI DNDPSCKYAY EQNNRTRFIE KSISEVDGRE 70 80 90 100 110 120 LNALYPNNQH KILVGCAPCQ DFSQYTKKSR TGTKWQLLTE FSRLIREIEP DIISMENVPE 130 140 150 160 170 180 VRTFNRGEVF NNFIQSLEQL GYHVSHSVVH CPDYGIPQQR DRLVLFAAKQ GVIKIIPPTH 190 200 210 220 230 240 TPENYRTVRD VIGSLATNYS GGHWEGDSMH AASRLEDINL RRIQHSVPGG TWADWPEELI 250 260 270 280 290 300 AECHKKESGE SYGSVYGRME WDKVAPTITT QCNGYGNGRF GHPEQDRAIS LREAALLQTF 310 320 330 340 350 PRSYQFAPEG QLKFKTVSRQ IGNAVPVALG RVIAKSIKRF LEGLHERQRV RIII

References

[1]	"Cloning, sequence and characterization of m5C-methyltransferase-encoding gene, hgiDIIM (GTCGAC), from Herpetosiphon giganteus strain Hpa2." Duesterhoeft A., Kroeger M. Gene 106:87-92(1991) [PubMed: 1937045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPA2.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed: 7607523] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55141 Genomic DNA. Translation: CAA38941.1.
PIR	JT0594. S21949.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3418. M.HgiDII.
Enzyme and pathway databases
BRENDA	2.1.1.37. 291850.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTD2_HERAU

Accession

Primary (citable) accession number: P25265

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	March 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)