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P25265

- MTD2_HERAU

UniProt

P25265 - MTD2_HERAU

Protein

Modification methylase HgiDII

Gene

hgiDIIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei79 – 791PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3418. M.HgiDII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HgiDII (EC:2.1.1.37)
    Short name:
    M.HgiDII
    Alternative name(s):
    Cytosine-specific methyltransferase HgiDII
    Gene namesi
    Name:hgiDIIM
    OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
    Taxonomic identifieri65 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Modification methylase HgiDIIPRO_0000087889Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP25265.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 344342SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25265-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVGAVIDLFC GVGGLTHGLI LEGFGVLAGI DNDPSCKYAY EQNNRTRFIE    50
    KSISEVDGRE LNALYPNNQH KILVGCAPCQ DFSQYTKKSR TGTKWQLLTE 100
    FSRLIREIEP DIISMENVPE VRTFNRGEVF NNFIQSLEQL GYHVSHSVVH 150
    CPDYGIPQQR DRLVLFAAKQ GVIKIIPPTH TPENYRTVRD VIGSLATNYS 200
    GGHWEGDSMH AASRLEDINL RRIQHSVPGG TWADWPEELI AECHKKESGE 250
    SYGSVYGRME WDKVAPTITT QCNGYGNGRF GHPEQDRAIS LREAALLQTF 300
    PRSYQFAPEG QLKFKTVSRQ IGNAVPVALG RVIAKSIKRF LEGLHERQRV 350
    RIII 354
    Length:354
    Mass (Da):39,853
    Last modified:May 1, 1992 - v1
    Checksum:iBDA017785D5ADC75
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55141 Genomic DNA. Translation: CAA38941.1.
    PIRiS21949. JT0594.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55141 Genomic DNA. Translation: CAA38941.1 .
    PIRi S21949. JT0594.

    3D structure databases

    ProteinModelPortali P25265.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3418. M.HgiDII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequence and characterization of m5C-methyltransferase-encoding gene, hgiDIIM (GTCGAC), from Herpetosiphon giganteus strain Hpa2."
      Duesterhoeft A., Kroeger M.
      Gene 106:87-92(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HPA2.
    2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
      Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
      Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiMTD2_HERAU
    AccessioniPrimary (citable) accession number: P25265
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3