Reviewed, UniProtKB/Swiss-Prot P25264 (MTC2_HERAU)

Names and origin

Protein names

Recommended name:
    Modification methylase HgiCII
      Short name=M.HgiCII
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase HgiCII

Gene names

Name:

hgiCIIM

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiCII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Modification methylase HgiCII		PRO_0000087887
Sites
	Active site	75	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25264-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 721217050B382081	FASTA	437	49,622
	10 20 30 40 50 60 MKQFRFIDLF AGIGGFRLGL EAVGGICVGS AEIDQQAIKV YRQNWPTDRS EHNLGDITTL 70 80 90 100 110 120 QQLPAHDLVV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR LVRINQPKAF IFENVKGLID 130 140 150 160 170 180 PRNRLCLESI LDSFKAEGYN VYYKLLNSFD YGVAQNRDRV FIIGIQQKLG VPDFSFPEYS 190 200 210 220 230 240 ESEQRLYDIL DNLQTPSIIP ESLPIQRNLF GERIEVGFNK LTPRGAFNDF FILNDIRNGP 250 260 270 280 290 300 TSIHSWEIYA TTEREKQICT TIMRNRGNPR YGDCDGNPMS YQDIADLVVD LAESELQVLI 310 320 330 340 350 360 QKRILRQYED GKYEFFNRRL SGGIDGTYRI FLPHARFFGR LTARGMHDEI AEISVSGATA 370 380 390 400 410 420 EAYKQNFIQQ ILIPKRHRPI TVNEAARIQG FPATFKFHSN QSANFRLIGN SVAPPVIMAL 430 GKALPNDHLF EPELCEV

References

[1]	"Stepwise cloning and genetic organization of the seemingly unclonable HgiCII restriction-modification system from Herpetosiphon giganteus strain Hpg9, using PCR technique." Erdmann D., Horst G., Duesterhoeft A., Kroeger M. Gene 117:15-22(1992) [PubMed: 1644308] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPG9.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed: 7607523] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55139 Genomic DNA. Translation: CAA38935.1.
PIR	JC1165.
3D structure databases
HSSP	HSSP built from PDB template 1FJX based on UniProtKB P05102.
ModBase	Search...
Protein family/group databases
REBASE	3416. M.HgiCII.
Enzyme and pathway databases
BRENDA	2.1.1.37. 291850.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTC2_HERAU

Accession

Primary (citable) accession number: P25264

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	March 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)