Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P25264

- MTC2_HERAU

UniProt

P25264 - MTC2_HERAU

Protein

Modification methylase HgiCII

Gene

hgiCIIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiCII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei75 – 751PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3416. M.HgiCII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HgiCII (EC:2.1.1.37)
    Short name:
    M.HgiCII
    Alternative name(s):
    Cytosine-specific methyltransferase HgiCII
    Gene namesi
    Name:hgiCIIM
    OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
    Taxonomic identifieri65 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 437437Modification methylase HgiCIIPRO_0000087887Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP25264.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 431428SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25264-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQFRFIDLF AGIGGFRLGL EAVGGICVGS AEIDQQAIKV YRQNWPTDRS    50
    EHNLGDITTL QQLPAHDLVV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR 100
    LVRINQPKAF IFENVKGLID PRNRLCLESI LDSFKAEGYN VYYKLLNSFD 150
    YGVAQNRDRV FIIGIQQKLG VPDFSFPEYS ESEQRLYDIL DNLQTPSIIP 200
    ESLPIQRNLF GERIEVGFNK LTPRGAFNDF FILNDIRNGP TSIHSWEIYA 250
    TTEREKQICT TIMRNRGNPR YGDCDGNPMS YQDIADLVVD LAESELQVLI 300
    QKRILRQYED GKYEFFNRRL SGGIDGTYRI FLPHARFFGR LTARGMHDEI 350
    AEISVSGATA EAYKQNFIQQ ILIPKRHRPI TVNEAARIQG FPATFKFHSN 400
    QSANFRLIGN SVAPPVIMAL GKALPNDHLF EPELCEV 437
    Length:437
    Mass (Da):49,622
    Last modified:May 1, 1992 - v1
    Checksum:i721217050B382081
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55139 Genomic DNA. Translation: CAA38935.1.
    PIRiJC1165.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55139 Genomic DNA. Translation: CAA38935.1 .
    PIRi JC1165.

    3D structure databases

    ProteinModelPortali P25264.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3416. M.HgiCII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Stepwise cloning and genetic organization of the seemingly unclonable HgiCII restriction-modification system from Herpetosiphon giganteus strain Hpg9, using PCR technique."
      Erdmann D., Horst G., Duesterhoeft A., Kroeger M.
      Gene 117:15-22(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HPG9.
    2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
      Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
      Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiMTC2_HERAU
    AccessioniPrimary (citable) accession number: P25264
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3