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P25264

- MTC2_HERAU

UniProt

P25264 - MTC2_HERAU

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Protein

Modification methylase HgiCII

Gene

hgiCIIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiCII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3416. M.HgiCII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgiCII (EC:2.1.1.37)
Short name:
M.HgiCII
Alternative name(s):
Cytosine-specific methyltransferase HgiCII
Gene namesi
Name:hgiCIIM
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Modification methylase HgiCIIPRO_0000087887Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP25264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 431428SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25264 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQFRFIDLF AGIGGFRLGL EAVGGICVGS AEIDQQAIKV YRQNWPTDRS
60 70 80 90 100
EHNLGDITTL QQLPAHDLVV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR
110 120 130 140 150
LVRINQPKAF IFENVKGLID PRNRLCLESI LDSFKAEGYN VYYKLLNSFD
160 170 180 190 200
YGVAQNRDRV FIIGIQQKLG VPDFSFPEYS ESEQRLYDIL DNLQTPSIIP
210 220 230 240 250
ESLPIQRNLF GERIEVGFNK LTPRGAFNDF FILNDIRNGP TSIHSWEIYA
260 270 280 290 300
TTEREKQICT TIMRNRGNPR YGDCDGNPMS YQDIADLVVD LAESELQVLI
310 320 330 340 350
QKRILRQYED GKYEFFNRRL SGGIDGTYRI FLPHARFFGR LTARGMHDEI
360 370 380 390 400
AEISVSGATA EAYKQNFIQQ ILIPKRHRPI TVNEAARIQG FPATFKFHSN
410 420 430
QSANFRLIGN SVAPPVIMAL GKALPNDHLF EPELCEV
Length:437
Mass (Da):49,622
Last modified:May 1, 1992 - v1
Checksum:i721217050B382081
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55139 Genomic DNA. Translation: CAA38935.1.
PIRiJC1165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55139 Genomic DNA. Translation: CAA38935.1 .
PIRi JC1165.

3D structure databases

ProteinModelPortali P25264.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3416. M.HgiCII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Stepwise cloning and genetic organization of the seemingly unclonable HgiCII restriction-modification system from Herpetosiphon giganteus strain Hpg9, using PCR technique."
    Erdmann D., Horst G., Duesterhoeft A., Kroeger M.
    Gene 117:15-22(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPG9.
  2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
    Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
    Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiMTC2_HERAU
AccessioniPrimary (citable) accession number: P25264
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3