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P25263

- MTC1_HERAU

UniProt

P25263 - MTC1_HERAU

Protein

Modification methylase HgiCI

Gene

hgiCIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GGYRCC, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the HgiCI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei75 – 751PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3415. M.HgiCI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HgiCI (EC:2.1.1.37)
    Short name:
    M.HgiCI
    Alternative name(s):
    Cytosine-specific methyltransferase HgiCI
    Gene namesi
    Name:hgiCIM
    OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
    Taxonomic identifieri65 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420Modification methylase HgiCIPRO_0000087886Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP25263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 417416SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25263-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKFIDLFAG IGGMRLGFEQ AMHELGIETA CVLSSEIDKH AQTTYAMNFH    50
    EQSQGDITQI QDFPSFDFLL AGFPCQPFSY AGKQKGFGDT RGTLFFEIER 100
    ILKAYRPKGF LLENVRGLTT HDKGRTFKTI LQKLHELNYG VYLILNSSNF 150
    QVPQNRLRVY IVGLDQSQPE LTITSHIGAT DSHKFKQLSN QASLFDTNKI 200
    MLVRDILEDH PLDKYNCSTD FVNKLLAFIG HPIKLNGKRL IDYRNGNSIH 250
    SWELGIKGEC TSDEIQFMNA LIANRRKKHF GAHQDGKKLT IEQIKTFFEH 300
    DDLDSIMQSL ITKGYLQEVN GRFNPVAGNM SFEVFKFLDP DSVSITLVSS 350
    DAHKIGVVHQ NRIRRITPRE CARLQGFPDS FQFHPKDSLA YRQFGNSVSV 400
    PVVKAVILDL FKSADLASCF 420
    Length:420
    Mass (Da):47,641
    Last modified:November 1, 1995 - v2
    Checksum:iEDB427C9D66CD971
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55138 Genomic DNA. Translation: CAA38933.1.
    PIRiS19707.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55138 Genomic DNA. Translation: CAA38933.1 .
    PIRi S19707.

    3D structure databases

    ProteinModelPortali P25263.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3415. M.HgiCI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and molecular characterization of the HgiCI restriction/modification system from Herpetosiphon giganteus Hpg9 reveals high similarity to BanI."
      Erdmann D., Duesterhoeft A., Kroeger M.
      Eur. J. Biochem. 202:1247-1256(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HPG9.
    2. Kroeger M.
      Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
      Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
      Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiMTC1_HERAU
    AccessioniPrimary (citable) accession number: P25263
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 68 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3