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P25262 (MTB1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HgiBI

Short name=M.HgiBI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase HgiBI
Gene names
Name:hgiBIM
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiBI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 437437Modification methylase HgiBI
PRO_0000087885

Regions

Domain4 – 431428SAM-dependent MTase C5-type

Sites

Active site751 By similarity

Sequences

Sequence LengthMass (Da)Tools
P25262 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 70A658159D8D23AD

FASTA43749,627
        10         20         30         40         50         60 
MQQFRFIDLF AGIGGFRLGL EAVGGVCVAS AEIDQQAIKV YRQNWPTDGV DHNLGDITAI 

        70         80         90        100        110        120 
QQLPAHDVLV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR LVQINQPKAF IFENVKGLVD 

       130        140        150        160        170        180 
PRNRLCLEII LDSFKDLGYS VFYKLLNSFD FGVAQNRDRV FIVGIQQKLD LNGFSFPEYT 

       190        200        210        220        230        240 
ESEQRLYHIL DNLEVPETKL ESIPIQRNLF GERIDVGYNK LTPRGAFNDF FILNDIRNGP 

       250        260        270        280        290        300 
TSIHSWEIYP TTEREKQICM IIMRNRRNSR YGDCDGNPMS YQDIAELVAG LAEKELQTLV 

       310        320        330        340        350        360 
EKRILRQYPD GKYEFFNRRL SGGIDGTYRI FLPNARFFGT LTARGMHDEI AEISVSGANA 

       370        380        390        400        410        420 
EEYKHNFIQQ VLIPKRYRKI TVSEAARLQG FPGSFQFHSN QSANFRLIGN SVAPPVIVAL 

       430 
GKALQCVKLF EQELCEV 

« Hide

References

[1]"Isolation and genetic structure of the AvaII isoschizomeric restriction-modification system HgiBI from Herpetosiphon giganteus Hpg5: M.HgiBI reveals high homology to M.BanI."
Duesterhoeft A., Erdmann D., Kroeger M.
Nucleic Acids Res. 19:3207-3211(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPG5.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55137 Genomic DNA. Translation: CAA38927.1.
PIRS22307.

3D structure databases

ProteinModelPortalP25262.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3414. M.HgiBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB1_HERAU
AccessionPrimary (citable) accession number: P25262
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries