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P25262

- MTB1_HERAU

UniProt

P25262 - MTB1_HERAU

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Protein
Modification methylase HgiBI
Gene
hgiBIM
Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiBI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3414. M.HgiBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgiBI (EC:2.1.1.37)
Short name:
M.HgiBI
Alternative name(s):
Cytosine-specific methyltransferase HgiBI
Gene namesi
Name:hgiBIM
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Modification methylase HgiBI
PRO_0000087885Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP25262.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 431428SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25262-1 [UniParc]FASTAAdd to Basket

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MQQFRFIDLF AGIGGFRLGL EAVGGVCVAS AEIDQQAIKV YRQNWPTDGV    50
DHNLGDITAI QQLPAHDVLV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR 100
LVQINQPKAF IFENVKGLVD PRNRLCLEII LDSFKDLGYS VFYKLLNSFD 150
FGVAQNRDRV FIVGIQQKLD LNGFSFPEYT ESEQRLYHIL DNLEVPETKL 200
ESIPIQRNLF GERIDVGYNK LTPRGAFNDF FILNDIRNGP TSIHSWEIYP 250
TTEREKQICM IIMRNRRNSR YGDCDGNPMS YQDIAELVAG LAEKELQTLV 300
EKRILRQYPD GKYEFFNRRL SGGIDGTYRI FLPNARFFGT LTARGMHDEI 350
AEISVSGANA EEYKHNFIQQ VLIPKRYRKI TVSEAARLQG FPGSFQFHSN 400
QSANFRLIGN SVAPPVIVAL GKALQCVKLF EQELCEV 437
Length:437
Mass (Da):49,627
Last modified:May 1, 1992 - v1
Checksum:i70A658159D8D23AD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55137 Genomic DNA. Translation: CAA38927.1.
PIRiS22307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55137 Genomic DNA. Translation: CAA38927.1 .
PIRi S22307.

3D structure databases

ProteinModelPortali P25262.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3414. M.HgiBI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and genetic structure of the AvaII isoschizomeric restriction-modification system HgiBI from Herpetosiphon giganteus Hpg5: M.HgiBI reveals high homology to M.BanI."
    Duesterhoeft A., Erdmann D., Kroeger M.
    Nucleic Acids Res. 19:3207-3211(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPG5.
  2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
    Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
    Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiMTB1_HERAU
AccessioniPrimary (citable) accession number: P25262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 3, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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