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Protein

Modification methylase HgiBI

Gene

hgiBIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiBI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3414. M.HgiBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgiBI (EC:2.1.1.37)
Short name:
M.HgiBI
Alternative name(s):
Cytosine-specific methyltransferase HgiBI
Gene namesi
Name:hgiBIM
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Modification methylase HgiBIPRO_0000087885Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 431428SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQFRFIDLF AGIGGFRLGL EAVGGVCVAS AEIDQQAIKV YRQNWPTDGV
60 70 80 90 100
DHNLGDITAI QQLPAHDVLV GGVPCQPWSI AGKNQAFDDP RGQLWADVIR
110 120 130 140 150
LVQINQPKAF IFENVKGLVD PRNRLCLEII LDSFKDLGYS VFYKLLNSFD
160 170 180 190 200
FGVAQNRDRV FIVGIQQKLD LNGFSFPEYT ESEQRLYHIL DNLEVPETKL
210 220 230 240 250
ESIPIQRNLF GERIDVGYNK LTPRGAFNDF FILNDIRNGP TSIHSWEIYP
260 270 280 290 300
TTEREKQICM IIMRNRRNSR YGDCDGNPMS YQDIAELVAG LAEKELQTLV
310 320 330 340 350
EKRILRQYPD GKYEFFNRRL SGGIDGTYRI FLPNARFFGT LTARGMHDEI
360 370 380 390 400
AEISVSGANA EEYKHNFIQQ VLIPKRYRKI TVSEAARLQG FPGSFQFHSN
410 420 430
QSANFRLIGN SVAPPVIVAL GKALQCVKLF EQELCEV
Length:437
Mass (Da):49,627
Last modified:April 30, 1992 - v1
Checksum:i70A658159D8D23AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55137 Genomic DNA. Translation: CAA38927.1.
PIRiS22307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55137 Genomic DNA. Translation: CAA38927.1.
PIRiS22307.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3414. M.HgiBI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and genetic structure of the AvaII isoschizomeric restriction-modification system HgiBI from Herpetosiphon giganteus Hpg5: M.HgiBI reveals high homology to M.BanI."
    Duesterhoeft A., Erdmann D., Kroeger M.
    Nucleic Acids Res. 19:3207-3211(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPG5.
  2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
    Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
    Gene 157:43-47(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiMTB1_HERAU
AccessioniPrimary (citable) accession number: P25262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1992
Last sequence update: April 30, 1992
Last modified: March 31, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.