P25261 (T2G1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HgiGI
Short name=R.HgiGI
EC=3.1.21.4

Alternative name(s):
Endonuclease HgiGI
Type II restriction enzyme HgiGI

Gene names

Name:

hgiGIR

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	335 AA.
Sequence status	Fragment.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GRCGYC and cleaves after R-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 335	›335	Type-2 restriction enzyme HgiGI		PRO_0000077315
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P25261 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 180CEFF503A5C90A	FASTA	335	38,460
	10 20 30 40 50 60 AWGKNQFNNA FPIALACFMF SQQIKPIYIR LEKRNTEHNY IAVDQIFQIN PLDPQAFFAF 70 80 90 100 110 120 EHSYHPYTEL IIGKTPAIDV VISNLQNSQI INAFEIKLTA IPDNTTAILS DHLQGCEIVI 130 140 150 160 170 180 RPDTIVYLAL SIAKIFQQNS SVLLDILDPI CARISDWEDV TSIKPMIPVF CELLYTIFDR 190 200 210 220 230 240 YQSAQIPILL QPIWKTQGKL SILHENCLDL FVWSNFALAK VFLDASIKPS EKSITRPERT 250 260 270 280 290 300 TVWLIKMLYD FAQNGKIDYK RTLDRITFNL KNDKAFAASG MVTRKYMNSP ELHNPRIKRH 310 320 330 LIKHIIINGG QRYLSPERRL DSAIVSTPSL FEDGL

References

[1]	Duesterhoeft A., Kroeger M. Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPA1.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55143 Genomic DNA. Translation: CAA38946.1.
PIR	S21954.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1107. HgiGI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019044. Restrct_endonuc_II_HindVP. [Graphical view]
Pfam	PF09519. RE_HindVP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2G1_HERAU

Accession

Primary (citable) accession number: P25261

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program