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P25261 (T2G1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HgiGI

Short name=R.HgiGI
EC=3.1.21.4
Alternative name(s):
Endonuclease HgiGI
Type II restriction enzyme HgiGI
Gene names
Name:hgiGIR
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length335 AA.
Sequence statusFragment.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GRCGYC and cleaves after R-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 335›335Type-2 restriction enzyme HgiGI
PRO_0000077315

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P25261 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 180CEFF503A5C90A

FASTA33538,460
        10         20         30         40         50         60 
AWGKNQFNNA FPIALACFMF SQQIKPIYIR LEKRNTEHNY IAVDQIFQIN PLDPQAFFAF 

        70         80         90        100        110        120 
EHSYHPYTEL IIGKTPAIDV VISNLQNSQI INAFEIKLTA IPDNTTAILS DHLQGCEIVI 

       130        140        150        160        170        180 
RPDTIVYLAL SIAKIFQQNS SVLLDILDPI CARISDWEDV TSIKPMIPVF CELLYTIFDR 

       190        200        210        220        230        240 
YQSAQIPILL QPIWKTQGKL SILHENCLDL FVWSNFALAK VFLDASIKPS EKSITRPERT 

       250        260        270        280        290        300 
TVWLIKMLYD FAQNGKIDYK RTLDRITFNL KNDKAFAASG MVTRKYMNSP ELHNPRIKRH 

       310        320        330 
LIKHIIINGG QRYLSPERRL DSAIVSTPSL FEDGL 

« Hide

References

[1]Duesterhoeft A., Kroeger M.
Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPA1.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55143 Genomic DNA. Translation: CAA38946.1.
PIRS21954.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1107. HgiGI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019044. Restrct_endonuc_II_HindVP.
[Graphical view]
PfamPF09519. RE_HindVP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2G1_HERAU
AccessionPrimary (citable) accession number: P25261
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries