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P25261

- T2G1_HERAU

UniProt

P25261 - T2G1_HERAU

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Protein

Type-2 restriction enzyme HgiGI

Gene

hgiGIR

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence GRCGYC and cleaves after R-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1107. HgiGI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HgiGI (EC:3.1.21.4)
Short name:
R.HgiGI
Alternative name(s):
Endonuclease HgiGI
Type II restriction enzyme HgiGI
Gene namesi
Name:hgiGIR
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 335›335Type-2 restriction enzyme HgiGIPRO_0000077315Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019044. Restrct_endonuc_II_HindVP.
[Graphical view]
PfamiPF09519. RE_HindVP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P25261-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
AWGKNQFNNA FPIALACFMF SQQIKPIYIR LEKRNTEHNY IAVDQIFQIN
60 70 80 90 100
PLDPQAFFAF EHSYHPYTEL IIGKTPAIDV VISNLQNSQI INAFEIKLTA
110 120 130 140 150
IPDNTTAILS DHLQGCEIVI RPDTIVYLAL SIAKIFQQNS SVLLDILDPI
160 170 180 190 200
CARISDWEDV TSIKPMIPVF CELLYTIFDR YQSAQIPILL QPIWKTQGKL
210 220 230 240 250
SILHENCLDL FVWSNFALAK VFLDASIKPS EKSITRPERT TVWLIKMLYD
260 270 280 290 300
FAQNGKIDYK RTLDRITFNL KNDKAFAASG MVTRKYMNSP ELHNPRIKRH
310 320 330
LIKHIIINGG QRYLSPERRL DSAIVSTPSL FEDGL
Length:335
Mass (Da):38,460
Last modified:May 1, 1992 - v1
Checksum:i180CEFF503A5C90A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55143 Genomic DNA. Translation: CAA38946.1.
PIRiS21954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55143 Genomic DNA. Translation: CAA38946.1 .
PIRi S21954.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1107. HgiGI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019044. Restrct_endonuc_II_HindVP.
[Graphical view ]
Pfami PF09519. RE_HindVP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Duesterhoeft A., Kroeger M.
    Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPA1.
  2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
    Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
    Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiT2G1_HERAU
AccessioniPrimary (citable) accession number: P25261
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3