##gff-version 3 P25260 UniProtKB Chain 1 274 . . . ID=PRO_0000077314;Note=Type II restriction enzyme HgiEI P25260 UniProtKB Mutagenesis 176 176 . . . Note=Greatly decreased specific activity. Greatly increased specific activity%3B when associated with I-223. N->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7607523;Dbxref=PMID:7607523 P25260 UniProtKB Mutagenesis 223 223 . . . Note=Greatly increased specific activity%3B when associated with S-176. N->I;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7607523;Dbxref=PMID:7607523