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P25260 (T2E1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HgiEI

Short name=R.HgiEI
EC=3.1.21.4
Alternative name(s):
Endonuclease HgiEI
Type II restriction enzyme HgiEI
Gene names
Name:hgiEIR
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Type-2 restriction enzyme HgiEI
PRO_0000077314

Sequences

Sequence LengthMass (Da)Tools
P25260 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: B9F38236F2E83718

FASTA27431,325
        10         20         30         40         50         60 
MAINPITRNK IKDYLNSFIQ QQLSVYRERS LREFQDVDSY LPSLSKDGDL KPFHASLIPA 

        70         80         90        100        110        120 
SIMRLNRFER SLSTGLGSTF EECTRLIALD HHAVALRNYD IQAALDQAQW AAIDQLISII 

       130        140        150        160        170        180 
DRGLKHQTPS LNQMLEQIQS IPLTGILETH IVRADLYIQR HDGSELFFEI KSPKPNKGQC 

       190        200        210        220        230        240 
LEVMQRLLRI YTIKQQSAVP VKAFYAMAYN PWGISRASYR SSNTKKYTDF SNAVVIGQEF 

       250        260        270 
WSLIGEPSTY TELLEIYHEV GLAKSAEITQ KLLQ 

« Hide

References

[1]Erdmann D., Kroeger M.
Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPG24.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55142 Genomic DNA. Translation: CAA38945.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1104. HgiEI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view]
PfamPF09520. RE_TdeIII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2E1_HERAU
AccessionPrimary (citable) accession number: P25260
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries