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P25260

- T2E1_HERAU

UniProt

P25260 - T2E1_HERAU

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Protein

Type-2 restriction enzyme HgiEI

Gene

hgiEIR

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1104. HgiEI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HgiEI (EC:3.1.21.4)
Short name:
R.HgiEI
Alternative name(s):
Endonuclease HgiEI
Type II restriction enzyme HgiEI
Gene namesi
Name:hgiEIR
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Type-2 restriction enzyme HgiEIPRO_0000077314Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view]
PfamiPF09520. RE_TdeIII. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25260-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAINPITRNK IKDYLNSFIQ QQLSVYRERS LREFQDVDSY LPSLSKDGDL
60 70 80 90 100
KPFHASLIPA SIMRLNRFER SLSTGLGSTF EECTRLIALD HHAVALRNYD
110 120 130 140 150
IQAALDQAQW AAIDQLISII DRGLKHQTPS LNQMLEQIQS IPLTGILETH
160 170 180 190 200
IVRADLYIQR HDGSELFFEI KSPKPNKGQC LEVMQRLLRI YTIKQQSAVP
210 220 230 240 250
VKAFYAMAYN PWGISRASYR SSNTKKYTDF SNAVVIGQEF WSLIGEPSTY
260 270
TELLEIYHEV GLAKSAEITQ KLLQ
Length:274
Mass (Da):31,325
Last modified:May 1, 1992 - v1
Checksum:iB9F38236F2E83718
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55142 Genomic DNA. Translation: CAA38945.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55142 Genomic DNA. Translation: CAA38945.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1104. HgiEI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view ]
Pfami PF09520. RE_TdeIII. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Erdmann D., Kroeger M.
    Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPG24.
  2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
    Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
    Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiT2E1_HERAU
AccessioniPrimary (citable) accession number: P25260
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3