Reviewed, UniProtKB/Swiss-Prot P25260 (T2E1_HERAU)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme HgiEI
      Short name=R.HgiEI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme HgiEI
    Endonuclease HgiEI

Gene names

Name:

hgiEIR

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGWCC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Type-2 restriction enzyme HgiEI		PRO_0000077314

Sequences

	Sequence		Length	Mass (Da)
	P25260-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: B9F38236F2E83718	FASTA	274	31,325
	10 20 30 40 50 60 MAINPITRNK IKDYLNSFIQ QQLSVYRERS LREFQDVDSY LPSLSKDGDL KPFHASLIPA 70 80 90 100 110 120 SIMRLNRFER SLSTGLGSTF EECTRLIALD HHAVALRNYD IQAALDQAQW AAIDQLISII 130 140 150 160 170 180 DRGLKHQTPS LNQMLEQIQS IPLTGILETH IVRADLYIQR HDGSELFFEI KSPKPNKGQC 190 200 210 220 230 240 LEVMQRLLRI YTIKQQSAVP VKAFYAMAYN PWGISRASYR SSNTKKYTDF SNAVVIGQEF 250 260 270 WSLIGEPSTY TELLEIYHEV GLAKSAEITQ KLLQ

References

[1]	Erdmann D., Kroeger M. Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPG24.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed: 7607523] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55142 Genomic DNA. Translation: CAA38945.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1104. HgiEI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 291850.
Family and domain databases
InterPro	IPR019045. Restrict_endonuc_II_MjaII. [Graphical view]
Pfam	PF09520. RE_MjaII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2E1_HERAU

Accession

Primary (citable) accession number: P25260

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)