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P25260

- T2E1_HERAU

UniProt

P25260 - T2E1_HERAU

Protein

Type-2 restriction enzyme HgiEI

Gene

hgiEIR

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 56 (03 Sep 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1104. HgiEI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HgiEI (EC:3.1.21.4)
    Short name:
    R.HgiEI
    Alternative name(s):
    Endonuclease HgiEI
    Type II restriction enzyme HgiEI
    Gene namesi
    Name:hgiEIR
    OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
    Taxonomic identifieri65 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 274274Type-2 restriction enzyme HgiEIPRO_0000077314Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019045. Restrct_endonuc_II_TdeIII.
    [Graphical view]
    PfamiPF09520. RE_TdeIII. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25260-1 [UniParc]FASTAAdd to Basket

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    MAINPITRNK IKDYLNSFIQ QQLSVYRERS LREFQDVDSY LPSLSKDGDL    50
    KPFHASLIPA SIMRLNRFER SLSTGLGSTF EECTRLIALD HHAVALRNYD 100
    IQAALDQAQW AAIDQLISII DRGLKHQTPS LNQMLEQIQS IPLTGILETH 150
    IVRADLYIQR HDGSELFFEI KSPKPNKGQC LEVMQRLLRI YTIKQQSAVP 200
    VKAFYAMAYN PWGISRASYR SSNTKKYTDF SNAVVIGQEF WSLIGEPSTY 250
    TELLEIYHEV GLAKSAEITQ KLLQ 274
    Length:274
    Mass (Da):31,325
    Last modified:May 1, 1992 - v1
    Checksum:iB9F38236F2E83718
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55142 Genomic DNA. Translation: CAA38945.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55142 Genomic DNA. Translation: CAA38945.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1104. HgiEI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019045. Restrct_endonuc_II_TdeIII.
    [Graphical view ]
    Pfami PF09520. RE_TdeIII. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Erdmann D., Kroeger M.
      Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HPG24.
    2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
      Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
      Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiT2E1_HERAU
    AccessioniPrimary (citable) accession number: P25260
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: September 3, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3