Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P25259 (T2C2_HERAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HgiCII

Short name=R.HgiCII
EC=3.1.21.4
Alternative name(s):
Endonuclease HgiCII
Type II restriction enzyme HgiCII
Gene names
Name:hgiCIIR
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Type-2 restriction enzyme HgiCII
PRO_0000077312

Sequences

Sequence LengthMass (Da)Tools
P25259 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 0403DF0B9F564619

FASTA27331,056
        10         20         30         40         50         60 
MSINPITRNK IKDYLNGFID QQLAVYSQRN LREFHDVDSY LAAISSDGDL KPFHASIIPS 

        70         80         90        100        110        120 
AIMRLNRFER SLSTGLGSTF EECARVIALD HHAVAIRSYD IHTSLDQAVW ASIDLLISNI 

       130        140        150        160        170        180 
DRNNQRQIPS ITEMLEKLQS IALTGIAENH VVRADLYVQR HDGSELFFEI KSPKPNKGQC 

       190        200        210        220        230        240 
LEVMQRLLRI YAIKQNSTLP THAFYAMAYN PWGANRASYT YSIVKKYTDF TNAVVIGQEF 

       250        260        270 
WSLIGESSTY TELLEIYREV GLSKSSEITK KLL 

« Hide

References

[1]"Stepwise cloning and genetic organization of the seemingly unclonable HgiCII restriction-modification system from Herpetosiphon giganteus strain Hpg9, using PCR technique."
Erdmann D., Horst G., Duesterhoeft A., Kroeger M.
Gene 117:15-22(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPG9.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55139 Genomic DNA. Translation: CAA38936.1.
PIRJC1166.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1100. HgiCII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view]
PfamPF09520. RE_TdeIII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2C2_HERAU
AccessionPrimary (citable) accession number: P25259
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries