P25259 (T2C2_HERAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HgiCII
Short name=R.HgiCII
EC=3.1.21.4

Alternative name(s):
Endonuclease HgiCII
Type II restriction enzyme HgiCII

Gene names

Name:

hgiCIIR

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGWCC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Type-2 restriction enzyme HgiCII		PRO_0000077312

Sequences

	Sequence		Length	Mass (Da)
	P25259 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 0403DF0B9F564619	FASTA	273	31,056
	10 20 30 40 50 60 MSINPITRNK IKDYLNGFID QQLAVYSQRN LREFHDVDSY LAAISSDGDL KPFHASIIPS 70 80 90 100 110 120 AIMRLNRFER SLSTGLGSTF EECARVIALD HHAVAIRSYD IHTSLDQAVW ASIDLLISNI 130 140 150 160 170 180 DRNNQRQIPS ITEMLEKLQS IALTGIAENH VVRADLYVQR HDGSELFFEI KSPKPNKGQC 190 200 210 220 230 240 LEVMQRLLRI YAIKQNSTLP THAFYAMAYN PWGANRASYT YSIVKKYTDF TNAVVIGQEF 250 260 270 WSLIGESSTY TELLEIYREV GLSKSSEITK KLL

References

[1]	"Stepwise cloning and genetic organization of the seemingly unclonable HgiCII restriction-modification system from Herpetosiphon giganteus strain Hpg9, using PCR technique." Erdmann D., Horst G., Duesterhoeft A., Kroeger M. Gene 117:15-22(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPG9.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55139 Genomic DNA. Translation: CAA38936.1.
PIR	JC1166.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1100. HgiCII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019045. Restrct_endonuc_II_MjaII. [Graphical view]
Pfam	PF09520. RE_MjaII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2C2_HERAU

Accession

Primary (citable) accession number: P25259

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program