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Protein

Type-2 restriction enzyme HgiCII

Gene

hgiCIIR

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1100. HgiCII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HgiCII (EC:3.1.21.4)
Short name:
R.HgiCII
Alternative name(s):
Endonuclease HgiCII
Type II restriction enzyme HgiCII
Gene namesi
Name:hgiCIIR
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Type-2 restriction enzyme HgiCIIPRO_0000077312Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view]
PfamiPF09520. RE_TdeIII. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25259-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSINPITRNK IKDYLNGFID QQLAVYSQRN LREFHDVDSY LAAISSDGDL
60 70 80 90 100
KPFHASIIPS AIMRLNRFER SLSTGLGSTF EECARVIALD HHAVAIRSYD
110 120 130 140 150
IHTSLDQAVW ASIDLLISNI DRNNQRQIPS ITEMLEKLQS IALTGIAENH
160 170 180 190 200
VVRADLYVQR HDGSELFFEI KSPKPNKGQC LEVMQRLLRI YAIKQNSTLP
210 220 230 240 250
THAFYAMAYN PWGANRASYT YSIVKKYTDF TNAVVIGQEF WSLIGESSTY
260 270
TELLEIYREV GLSKSSEITK KLL
Length:273
Mass (Da):31,056
Last modified:May 1, 1992 - v1
Checksum:i0403DF0B9F564619
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55139 Genomic DNA. Translation: CAA38936.1.
PIRiJC1166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55139 Genomic DNA. Translation: CAA38936.1.
PIRiJC1166.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1100. HgiCII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view]
PfamiPF09520. RE_TdeIII. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Stepwise cloning and genetic organization of the seemingly unclonable HgiCII restriction-modification system from Herpetosiphon giganteus strain Hpg9, using PCR technique."
    Erdmann D., Horst G., Duesterhoeft A., Kroeger M.
    Gene 117:15-22(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPG9.
  2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
    Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
    Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiT2C2_HERAU
AccessioniPrimary (citable) accession number: P25259
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.