ID   T2C1_HERAU              Reviewed;         345 AA.
AC   P25258;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   28-JUN-2023, entry version 58.
DE   RecName: Full=Type II restriction enzyme HgiCI {ECO:0000303|PubMed:12654995};
DE            Short=R.HgiCI {ECO:0000303|PubMed:1662609};
DE            EC=3.1.21.4 {ECO:0000269|PubMed:1662609};
DE   AltName: Full=Endonuclease HgiCI;
DE   AltName: Full=Type-2 restriction enzyme HgiCI;
GN   Name=hgiCIR;
OS   Herpetosiphon aurantiacus (Herpetosiphon giganteus).
OC   Bacteria; Chloroflexota; Chloroflexia; Herpetosiphonales;
OC   Herpetosiphonaceae; Herpetosiphon.
OX   NCBI_TaxID=65;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=HPG9;
RX   PubMed=1662609; DOI=10.1111/j.1432-1033.1991.tb16497.x;
RA   Erdmann D., Duesterhoeft A., Kroeger M.;
RT   "Cloning and molecular characterization of the HgiCI
RT   restriction/modification system from Herpetosiphon giganteus Hpg9 reveals
RT   high similarity to BanI.";
RL   Eur. J. Biochem. 202:1247-1256(1991).
RN   [2]
RP   DISCUSSION OF SEQUENCE.
RX   PubMed=7607523; DOI=10.1016/0378-1119(94)00779-r;
RA   Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S.,
RA   Meyer-Rogge S., Moestl D.;
RT   "Organization and gene expression within restriction-modification systems
RT   of Herpetosiphon giganteus.";
RL   Gene 157:43-47(1995).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GGYRCC-3' and cleaves after G-1.
CC       {ECO:0000269|PubMed:1662609, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000269|PubMed:1662609};
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DR   EMBL; X55138; CAA38932.1; -; Genomic_DNA.
DR   PIR; S19706; S19706.
DR   AlphaFoldDB; P25258; -.
DR   REBASE; 1099; HgiCI.
DR   PRO; PR:P25258; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..345
FT                   /note="Type II restriction enzyme HgiCI"
FT                   /id="PRO_0000077311"
SQ   SEQUENCE   345 AA;  39170 MW;  BAE97E3AFEB89EB1 CRC64;
     MNYQRSFEDL EFNAIKWWPQ ELSATVAEAS VLPILISSQD LFISILKLSG THPEQIFDVI
     NAAQISANLF LKHLVVLADY GGEMIKRLGK EFQEIFPRMD STLEYYMNYT FKGEQYTYIF
     KKLPIKGLDN SKLAIDGKAI IEIKPLSDLY RDMIMILLYG STTEQFNLAG LEKCEIGTIL
     GKNEIIYTYI TQKYLYVSRI TNGANTNSLG QIAQTYVCDI LSKYLPNDYS VTRNGKILLS
     DLNSQDSTKT SFDILVEFAD KKVGIEVSFQ VTTNSTIERK AGQARDRQNR MHAHYYWIAY
     VIDGAGNFER SGAVRAICRY SDCTVAYSES EIAVLAAFIQ EKFNA
//