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P25258 (T2C1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HgiCI

Short name=R.HgiCI
EC=3.1.21.4
Alternative name(s):
Endonuclease HgiCI
Type II restriction enzyme HgiCI
Gene names
Name:hgiCIR
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGYRCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Type-2 restriction enzyme HgiCI
PRO_0000077311

Sequences

Sequence LengthMass (Da)Tools
P25258 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: BAE97E3AFEB89EB1

FASTA34539,170
        10         20         30         40         50         60 
MNYQRSFEDL EFNAIKWWPQ ELSATVAEAS VLPILISSQD LFISILKLSG THPEQIFDVI 

        70         80         90        100        110        120 
NAAQISANLF LKHLVVLADY GGEMIKRLGK EFQEIFPRMD STLEYYMNYT FKGEQYTYIF 

       130        140        150        160        170        180 
KKLPIKGLDN SKLAIDGKAI IEIKPLSDLY RDMIMILLYG STTEQFNLAG LEKCEIGTIL 

       190        200        210        220        230        240 
GKNEIIYTYI TQKYLYVSRI TNGANTNSLG QIAQTYVCDI LSKYLPNDYS VTRNGKILLS 

       250        260        270        280        290        300 
DLNSQDSTKT SFDILVEFAD KKVGIEVSFQ VTTNSTIERK AGQARDRQNR MHAHYYWIAY 

       310        320        330        340 
VIDGAGNFER SGAVRAICRY SDCTVAYSES EIAVLAAFIQ EKFNA 

« Hide

References

[1]"Cloning and molecular characterization of the HgiCI restriction/modification system from Herpetosiphon giganteus Hpg9 reveals high similarity to BanI."
Erdmann D., Duesterhoeft A., Kroeger M.
Eur. J. Biochem. 202:1247-1256(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPG9.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55138 Genomic DNA. Translation: CAA38932.1.
PIRS19706.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1099. HgiCI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2C1_HERAU
AccessionPrimary (citable) accession number: P25258
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries