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P25257 (T2B1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HgiBI

Short name=R.HgiBI
EC=3.1.21.4
Alternative name(s):
Endonuclease HgiBI
Type II restriction enzyme HgiBI
Gene names
Name:hgiBIR
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Type-2 restriction enzyme HgiBI
PRO_0000077310

Sequences

Sequence LengthMass (Da)Tools
P25257 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 301A50CD604A56FC

FASTA27431,189
        10         20         30         40         50         60 
MAINPITRNK IKDYLNSFIQ QQLSVYSQRS LREFQDVDSY PSSLSKDGDL KPFHASLIPA 

        70         80         90        100        110        120 
SIMRLNRFER SLSTGLGSTF EECTRLIALD HHAVALRNYD IQAALDQAQW AAIDQLISTI 

       130        140        150        160        170        180 
DRGLKHQTPS LNQMLEQIQS IPLTGILETH IVRADLYIQR HDGSELFFEI KSPKPSKGQC 

       190        200        210        220        230        240 
LEVMQRLLRI YTIKQQSAVP VKAFYAMAYN PWGISRASYR SSITKKYTDF SNAVVIGQEF 

       250        260        270 
WSLIGEPSTY TELLEIYHEV GLAKSAEITQ KLLQ 

« Hide

References

[1]"Isolation and genetic structure of the AvaII isoschizomeric restriction-modification system HgiBI from Herpetosiphon giganteus Hpg5: M.HgiBI reveals high homology to M.BanI."
Duesterhoeft A., Erdmann D., Kroeger M.
Nucleic Acids Res. 19:3207-3211(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPG5.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55137 Genomic DNA. Translation: CAA38928.1.
PIRS22308.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1098. HgiBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view]
PfamPF09520. RE_TdeIII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2B1_HERAU
AccessionPrimary (citable) accession number: P25257
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries