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P25257

- T2B1_HERAU

UniProt

P25257 - T2B1_HERAU

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Protein

Type-2 restriction enzyme HgiBI

Gene
hgiBIR
Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1098. HgiBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HgiBI (EC:3.1.21.4)
Short name:
R.HgiBI
Alternative name(s):
Endonuclease HgiBI
Type II restriction enzyme HgiBI
Gene namesi
Name:hgiBIR
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Type-2 restriction enzyme HgiBIPRO_0000077310Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view]
PfamiPF09520. RE_TdeIII. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25257-1 [UniParc]FASTAAdd to Basket

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MAINPITRNK IKDYLNSFIQ QQLSVYSQRS LREFQDVDSY PSSLSKDGDL    50
KPFHASLIPA SIMRLNRFER SLSTGLGSTF EECTRLIALD HHAVALRNYD 100
IQAALDQAQW AAIDQLISTI DRGLKHQTPS LNQMLEQIQS IPLTGILETH 150
IVRADLYIQR HDGSELFFEI KSPKPSKGQC LEVMQRLLRI YTIKQQSAVP 200
VKAFYAMAYN PWGISRASYR SSITKKYTDF SNAVVIGQEF WSLIGEPSTY 250
TELLEIYHEV GLAKSAEITQ KLLQ 274
Length:274
Mass (Da):31,189
Last modified:May 1, 1992 - v1
Checksum:i301A50CD604A56FC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55137 Genomic DNA. Translation: CAA38928.1.
PIRiS22308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55137 Genomic DNA. Translation: CAA38928.1 .
PIRi S22308.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1098. HgiBI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019045. Restrct_endonuc_II_TdeIII.
[Graphical view ]
Pfami PF09520. RE_TdeIII. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and genetic structure of the AvaII isoschizomeric restriction-modification system HgiBI from Herpetosiphon giganteus Hpg5: M.HgiBI reveals high homology to M.BanI."
    Duesterhoeft A., Erdmann D., Kroeger M.
    Nucleic Acids Res. 19:3207-3211(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPG5.
  2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
    Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
    Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiT2B1_HERAU
AccessioniPrimary (citable) accession number: P25257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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