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P25257

- T2B1_HERAU

UniProt

P25257 - T2B1_HERAU

Protein

Type-2 restriction enzyme HgiBI

Gene

hgiBIR

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 56 (03 Sep 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1098. HgiBI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HgiBI (EC:3.1.21.4)
    Short name:
    R.HgiBI
    Alternative name(s):
    Endonuclease HgiBI
    Type II restriction enzyme HgiBI
    Gene namesi
    Name:hgiBIR
    OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
    Taxonomic identifieri65 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 274274Type-2 restriction enzyme HgiBIPRO_0000077310Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019045. Restrct_endonuc_II_TdeIII.
    [Graphical view]
    PfamiPF09520. RE_TdeIII. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25257-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAINPITRNK IKDYLNSFIQ QQLSVYSQRS LREFQDVDSY PSSLSKDGDL    50
    KPFHASLIPA SIMRLNRFER SLSTGLGSTF EECTRLIALD HHAVALRNYD 100
    IQAALDQAQW AAIDQLISTI DRGLKHQTPS LNQMLEQIQS IPLTGILETH 150
    IVRADLYIQR HDGSELFFEI KSPKPSKGQC LEVMQRLLRI YTIKQQSAVP 200
    VKAFYAMAYN PWGISRASYR SSITKKYTDF SNAVVIGQEF WSLIGEPSTY 250
    TELLEIYHEV GLAKSAEITQ KLLQ 274
    Length:274
    Mass (Da):31,189
    Last modified:May 1, 1992 - v1
    Checksum:i301A50CD604A56FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55137 Genomic DNA. Translation: CAA38928.1.
    PIRiS22308.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55137 Genomic DNA. Translation: CAA38928.1 .
    PIRi S22308.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1098. HgiBI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019045. Restrct_endonuc_II_TdeIII.
    [Graphical view ]
    Pfami PF09520. RE_TdeIII. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and genetic structure of the AvaII isoschizomeric restriction-modification system HgiBI from Herpetosiphon giganteus Hpg5: M.HgiBI reveals high homology to M.BanI."
      Duesterhoeft A., Erdmann D., Kroeger M.
      Nucleic Acids Res. 19:3207-3211(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HPG5.
    2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
      Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
      Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiT2B1_HERAU
    AccessioniPrimary (citable) accession number: P25257
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: September 3, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3