Reviewed, UniProtKB/Swiss-Prot P25257 (T2B1_HERAU)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme HgiBI
      Short name=R.HgiBI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme HgiBI
    Endonuclease HgiBI

Gene names

Name:

hgiBIR

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGWCC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Type-2 restriction enzyme HgiBI		PRO_0000077310

Sequences

	Sequence		Length	Mass (Da)
	P25257-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 301A50CD604A56FC	FASTA	274	31,189
	10 20 30 40 50 60 MAINPITRNK IKDYLNSFIQ QQLSVYSQRS LREFQDVDSY PSSLSKDGDL KPFHASLIPA 70 80 90 100 110 120 SIMRLNRFER SLSTGLGSTF EECTRLIALD HHAVALRNYD IQAALDQAQW AAIDQLISTI 130 140 150 160 170 180 DRGLKHQTPS LNQMLEQIQS IPLTGILETH IVRADLYIQR HDGSELFFEI KSPKPSKGQC 190 200 210 220 230 240 LEVMQRLLRI YTIKQQSAVP VKAFYAMAYN PWGISRASYR SSITKKYTDF SNAVVIGQEF 250 260 270 WSLIGEPSTY TELLEIYHEV GLAKSAEITQ KLLQ

References

[1]	"Isolation and genetic structure of the AvaII isoschizomeric restriction-modification system HgiBI from Herpetosiphon giganteus Hpg5: M.HgiBI reveals high homology to M.BanI." Duesterhoeft A., Erdmann D., Kroeger M. Nucleic Acids Res. 19:3207-3211(1991) [PubMed: 2062638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPG5.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed: 7607523] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55137 Genomic DNA. Translation: CAA38928.1.
PIR	S22308.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1098. HgiBI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 291850.
Family and domain databases
InterPro	IPR019045. Restrict_endonuc_II_MjaII. [Graphical view]
Pfam	PF09520. RE_MjaII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2B1_HERAU

Accession

Primary (citable) accession number: P25257

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)