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P25251

- CYSP4_BRANA

UniProt

P25251 - CYSP4_BRANA

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Protein

Cysteine proteinase COT44

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

May function in an early event in cortical cell differentiation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei124 – 1241By similarity
Active sitei260 – 2601By similarity
Active sitei280 – 2801By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Germination

Protein family/group databases

MEROPSiC01.021.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine proteinase COT44 (EC:3.4.22.-)
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei‹1 – 99›99Activation peptideSequence AnalysisPRO_0000026402Add
BLAST
Chaini100 – 328229Cysteine proteinase COT44PRO_0000026403Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi121 ↔ 163By similarity
Disulfide bondi155 ↔ 196By similarity
Disulfide bondi254 ↔ 305By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Present in both cotyledons and axes.

Developmental stagei

Expressed maximally during postgerminative growth.

Structurei

3D structure databases

ProteinModelPortaliP25251.
SMRiP25251. Positions 100-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25251-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIYLRWSLE HGKSNSNSNG IINQQDERFN IFKDNLRFID LHNENNKNAT
60 70 80 90 100
YKLGLTIFAN LTNDEYRSLY LGARTEPVRR ITKAKNVNMK YSAAVNVDEV
110 120 130 140 150
PVTVDWRQKG AVNAIKDQGT CGSCWAFSTA AAVEGINKIV TGELVSLSEQ
160 170 180 190 200
ELVDCDKSYN QGCNGGLMDY AFQFIMKNGG LNTEKDYPYH GTNGKCNSLL
210 220 230 240 250
KNSRVVTIDG YEDVPSKDET ALKRAVSYQP VSVAIDAGGR AFQHYQSGIF
260 270 280 290 300
TGKCGTNMDH AVVAVGYGSE NGVDYWIVRN SWGTRWGEDG YIRMERNVAS
310 320
KSGKCGIAIE ASYPVKYSPN PVRGTSSV
Length:328
Mass (Da):36,277
Last modified:May 1, 1992 - v1
Checksum:i9C95B3C488E8D0D6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

PIRiJQ1121.

Cross-referencesi

Sequence databases

PIRi JQ1121.

3D structure databases

ProteinModelPortali P25251.
SMRi P25251. Positions 100-320.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C01.021.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view ]
PANTHERi PTHR12411. PTHR12411. 1 hit.
Pfami PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view ]
PRINTSi PR00705. PAPAIN.
SMARTi SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view ]
PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Spatial patterns of gene expression in Brassica napus seedlings: identification of a cortex-specific gene and localization of mRNAs encoding isocitrate lyase and a polypeptide homologous to proteinases."
    Dietrich R.A., Maslyar D.J., Heupel R.C., Harada J.J.
    Plant Cell 1:73-80(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Seed.

Entry informationi

Entry nameiCYSP4_BRANA
AccessioniPrimary (citable) accession number: P25251
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 1, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3