P25251 (CYSP4_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cysteine proteinase COT44 EC=3.4.22.-
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	328 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function in an early event in cortical cell differentiation.
Tissue specificity	Present in both cotyledons and axes.
Developmental stage	Expressed maximally during postgerminative growth.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Biological process	Germination
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	‹1 – 99	›99	Activation peptide Potential		PRO_0000026402
	Chain	100 – 328	229	Cysteine proteinase COT44		PRO_0000026403
Sites
	Active site	124	1	By similarity
	Active site	260	1	By similarity
	Active site	280	1	By similarity
Amino acid modifications
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Disulfide bond	121 ↔ 163		By similarity
	Disulfide bond	155 ↔ 196		By similarity
	Disulfide bond	254 ↔ 305		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P25251 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 9C95B3C488E8D0D6	FASTA	328	36,277
	10 20 30 40 50 60 MSIYLRWSLE HGKSNSNSNG IINQQDERFN IFKDNLRFID LHNENNKNAT YKLGLTIFAN 70 80 90 100 110 120 LTNDEYRSLY LGARTEPVRR ITKAKNVNMK YSAAVNVDEV PVTVDWRQKG AVNAIKDQGT 130 140 150 160 170 180 CGSCWAFSTA AAVEGINKIV TGELVSLSEQ ELVDCDKSYN QGCNGGLMDY AFQFIMKNGG 190 200 210 220 230 240 LNTEKDYPYH GTNGKCNSLL KNSRVVTIDG YEDVPSKDET ALKRAVSYQP VSVAIDAGGR 250 260 270 280 290 300 AFQHYQSGIF TGKCGTNMDH AVVAVGYGSE NGVDYWIVRN SWGTRWGEDG YIRMERNVAS 310 320 KSGKCGIAIE ASYPVKYSPN PVRGTSSV

References

[1]

"Spatial patterns of gene expression in Brassica napus seedlings: identification of a cortex-specific gene and localization of mRNAs encoding isocitrate lyase and a polypeptide homologous to proteinases." Dietrich R.A., Maslyar D.J., Heupel R.C., Harada J.J. Plant Cell 1:73-80(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Seed.

Cross-references

Sequence databases
PIR	JQ1121.
3D structure databases
ProteinModelPortal	P25251.
SMR	P25251. Positions 100-320.
ModBase	Search...
Protein family/group databases
MEROPS	C01.021.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSP4_BRANA

Accession

Primary (citable) accession number: P25251

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program