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P25251 (CYSP4_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine proteinase COT44

EC=3.4.22.-
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length328 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May function in an early event in cortical cell differentiation.

Tissue specificity

Present in both cotyledons and axes.

Developmental stage

Expressed maximally during postgerminative growth.

Sequence similarities

Belongs to the peptidase C1 family.

Ontologies

Keywords
   Biological processGermination
   Molecular functionHydrolase
Protease
Thiol protease
   PTMDisulfide bond
Glycoprotein
Zymogen
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide‹1 – 99›99Activation peptide Potential
PRO_0000026402
Chain100 – 328229Cysteine proteinase COT44
PRO_0000026403

Sites

Active site1241 By similarity
Active site2601 By similarity
Active site2801 By similarity

Amino acid modifications

Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation601N-linked (GlcNAc...) Potential
Disulfide bond121 ↔ 163 By similarity
Disulfide bond155 ↔ 196 By similarity
Disulfide bond254 ↔ 305 By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P25251 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 9C95B3C488E8D0D6

FASTA32836,277
        10         20         30         40         50         60 
MSIYLRWSLE HGKSNSNSNG IINQQDERFN IFKDNLRFID LHNENNKNAT YKLGLTIFAN 

        70         80         90        100        110        120 
LTNDEYRSLY LGARTEPVRR ITKAKNVNMK YSAAVNVDEV PVTVDWRQKG AVNAIKDQGT 

       130        140        150        160        170        180 
CGSCWAFSTA AAVEGINKIV TGELVSLSEQ ELVDCDKSYN QGCNGGLMDY AFQFIMKNGG 

       190        200        210        220        230        240 
LNTEKDYPYH GTNGKCNSLL KNSRVVTIDG YEDVPSKDET ALKRAVSYQP VSVAIDAGGR 

       250        260        270        280        290        300 
AFQHYQSGIF TGKCGTNMDH AVVAVGYGSE NGVDYWIVRN SWGTRWGEDG YIRMERNVAS 

       310        320 
KSGKCGIAIE ASYPVKYSPN PVRGTSSV 

« Hide

References

[1]"Spatial patterns of gene expression in Brassica napus seedlings: identification of a cortex-specific gene and localization of mRNAs encoding isocitrate lyase and a polypeptide homologous to proteinases."
Dietrich R.A., Maslyar D.J., Heupel R.C., Harada J.J.
Plant Cell 1:73-80(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Seed.

Cross-references

Sequence databases

PIRJQ1121.

3D structure databases

ProteinModelPortalP25251.
SMRP25251. Positions 100-320.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC01.021.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERPTHR12411. PTHR12411. 1 hit.
PfamPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSPR00705. PAPAIN.
SMARTSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSP4_BRANA
AccessionPrimary (citable) accession number: P25251
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries