P25248 (ACEA_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Isocitrate lyase Short name=ICL Short name=Isocitrase Short name=Isocitratase EC=4.1.3.1
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in storage lipid mobilization during the growth of higher plant seedling.
Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer.
Subcellular location	Glyoxysome.
Developmental stage	Expressed maximally during postgerminative growth.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Isocitrate lyase		PRO_0000068802
Regions
	Motif	574 – 576	3	Microbody targeting signal Potential
Sites
	Active site	213	1	Probable
Experimental info
	Sequence conflict	75	1	A → V in CAA73792. Ref.3
	Sequence conflict	177	1	D → G in CAA73792. Ref.3
	Sequence conflict	256	1	A → P in CAA73792. Ref.3
	Sequence conflict	278	1	S → N in CAA73792. Ref.3
	Sequence conflict	340	1	N → T in CAA73792. Ref.3
	Sequence conflict	344	1	N → I in CAA73792. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P25248 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 81A87701A4ACC350	FASTA	576	64,326
	10 20 30 40 50 60 MAASFSVPSM IMEEEGRFEA EVAEVQTWWS SERFKLTRRP YTARDVVALR GHLKQGYASN 70 80 90 100 110 120 EMAKKLWRTL KSHQANGTAS RTFGALDPVQ VTMMAKHLDT IYVSGWQCSS THTSTNEPGP 130 140 150 160 170 180 DLADYPYDTV PNKVEHLFFA QQYHDRKQRE ARMSMSREER AKTPFVDYLK PIIADGDTGF 190 200 210 220 230 240 GGTTATVKLC KLFVERGAAG VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV 250 260 270 280 290 300 MGTETVLVAR TDAVAATLIQ SNIDSRDHQF ILGVTNPSLR GKSLSSLLAE GMAVGNNGPA 310 320 330 340 350 360 LQAIEDQWLS SARLMTFSDA VVEALKRMNL SENEKSRRVN EWLNHARYEN CLSNEQGREL 370 380 390 400 410 420 AAKLGVTDLF WDWDLPRTRE GFYRFQGSVT AAVVRGWAFA QIADLIWMET ASPDLNECTQ 430 440 450 460 470 480 FAEGVKSKTP EVMLAYNLSP SFNWDASGMT DQQMMEFIPR IARLGYCWQF ITLAGFHADA 490 500 510 520 530 540 LVVDTFAKDY ARRGMLAYVE RIQREERSNG VDTLAHQKWS GANYYDRYLK TVQGGISSTA 550 560 570 AMGKGVTEEQ FKETWTRPGA AGMGEGTSLV VAKSRM

References

[1]	"Coordinate expression of transcriptionally regulated isocitrate lyase and malate synthase genes in Brassica napus L." Comai L., Dietrich R.A., Maslyar D.J., Baden C.S., Harada J.J. Plant Cell 1:293-300(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Two classes of isocitrate lyase genes are expressed during late embryogeny and postgermination in Brassica napus L." Zhang J.Z., Gomez-Pedrozo M., Baden C.S., Harada J.J. Mol. Gen. Genet. 238:177-184(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Olesen C., Thomsen K.K., Svendsen I., Brandt A. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Global. Tissue: Cotyledon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08482 Genomic DNA. Translation: AAA32992.1. Y13356 mRNA. Translation: CAA73792.1.
PIR	WZRPI. JQ1105.
3D structure databases
ProteinModelPortal	P25248.
SMR	P25248. Positions 9-535.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_BRANA

Accession

Primary (citable) accession number: P25248
Secondary accession number(s): O04910

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program