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P25248 (ACEA_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate lyase

Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in storage lipid mobilization during the growth of higher plant seedling.

Catalytic activity

Isocitrate = succinate + glyoxylate.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.

Subunit structure

Homotetramer.

Subcellular location

Glyoxysome.

Developmental stage

Expressed maximally during postgerminative growth.

Sequence similarities

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisocitrate lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Isocitrate lyase
PRO_0000068802

Regions

Motif574 – 5763Microbody targeting signal Potential

Sites

Active site2131 Probable

Experimental info

Sequence conflict751A → V in CAA73792. Ref.3
Sequence conflict1771D → G in CAA73792. Ref.3
Sequence conflict2561A → P in CAA73792. Ref.3
Sequence conflict2781S → N in CAA73792. Ref.3
Sequence conflict3401N → T in CAA73792. Ref.3
Sequence conflict3441N → I in CAA73792. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P25248 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 81A87701A4ACC350

FASTA57664,326
        10         20         30         40         50         60 
MAASFSVPSM IMEEEGRFEA EVAEVQTWWS SERFKLTRRP YTARDVVALR GHLKQGYASN 

        70         80         90        100        110        120 
EMAKKLWRTL KSHQANGTAS RTFGALDPVQ VTMMAKHLDT IYVSGWQCSS THTSTNEPGP 

       130        140        150        160        170        180 
DLADYPYDTV PNKVEHLFFA QQYHDRKQRE ARMSMSREER AKTPFVDYLK PIIADGDTGF 

       190        200        210        220        230        240 
GGTTATVKLC KLFVERGAAG VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV 

       250        260        270        280        290        300 
MGTETVLVAR TDAVAATLIQ SNIDSRDHQF ILGVTNPSLR GKSLSSLLAE GMAVGNNGPA 

       310        320        330        340        350        360 
LQAIEDQWLS SARLMTFSDA VVEALKRMNL SENEKSRRVN EWLNHARYEN CLSNEQGREL 

       370        380        390        400        410        420 
AAKLGVTDLF WDWDLPRTRE GFYRFQGSVT AAVVRGWAFA QIADLIWMET ASPDLNECTQ 

       430        440        450        460        470        480 
FAEGVKSKTP EVMLAYNLSP SFNWDASGMT DQQMMEFIPR IARLGYCWQF ITLAGFHADA 

       490        500        510        520        530        540 
LVVDTFAKDY ARRGMLAYVE RIQREERSNG VDTLAHQKWS GANYYDRYLK TVQGGISSTA 

       550        560        570 
AMGKGVTEEQ FKETWTRPGA AGMGEGTSLV VAKSRM 

« Hide

References

[1]"Coordinate expression of transcriptionally regulated isocitrate lyase and malate synthase genes in Brassica napus L."
Comai L., Dietrich R.A., Maslyar D.J., Baden C.S., Harada J.J.
Plant Cell 1:293-300(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Two classes of isocitrate lyase genes are expressed during late embryogeny and postgermination in Brassica napus L."
Zhang J.Z., Gomez-Pedrozo M., Baden C.S., Harada J.J.
Mol. Gen. Genet. 238:177-184(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]Olesen C., Thomsen K.K., Svendsen I., Brandt A.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Global.
Tissue: Cotyledon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08482 Genomic DNA. Translation: AAA32992.1.
Y13356 mRNA. Translation: CAA73792.1.
PIRWZRPI. JQ1105.

3D structure databases

ProteinModelPortalP25248.
SMRP25248. Positions 9-535.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00703; UER00719.

Family and domain databases

Gene3D3.20.20.60. 2 hits.
InterProIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamPF00463. ICL. 1 hit.
[Graphical view]
PIRSFPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMSSF51621. SSF51621. 1 hit.
TIGRFAMsTIGR01346. isocit_lyase. 1 hit.
PROSITEPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACEA_BRANA
AccessionPrimary (citable) accession number: P25248
Secondary accession number(s): O04910
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 14, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways