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P25241 (T3RE_BACC1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type III restriction-modification system Bce10987IP enzyme res

EC=3.1.21.5
Gene names
Name:res
Synonyms:t3res
Ordered Locus Names:BCE_1019
OrganismBacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]
Taxonomic identifier222523 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length987 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This protein cuts the DNA outside of the recognition site. May also act as a helicase involved in unwinding DNA at the cleavage site. Protein only required for restriction but needs the presence of the modification enzyme By similarity.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium By similarity.

Subunit structure

Contains two different subunits: res and mod.

Sequence similarities

Belongs to the type III restriction-modification system res protein family.

Contains 1 VRR-NUC domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 987987Type III restriction-modification system Bce10987IP enzyme res
PRO_0000077376

Regions

Domain886 – 97489VRR-NUC

Experimental info

Sequence conflict410 – 46253TFERL…GYFGE → GDYQFIETKAPTGYDLNAKP IPFTITKGQAQVTSVTALNS LTTGSMELMKVDM in CAA43125. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P25241 [UniParc].

Last modified May 24, 2004. Version 2.
Checksum: 1D0D6CF4112407FC

FASTA987114,787
        10         20         30         40         50         60 
MKILLEELPH QEESLKAILE NFTGIDNAAN DSDVDYVYAN PLIRGRYNEI SNIDVKMETG 

        70         80         90        100        110        120 
TGKTYVYTRL MYEMHQQYGI FKFVIVVPSP AIKEGAKNFI QSDYAKQHFS QFYENVRIEL 

       130        140        150        160        170        180 
NVINAGDFKS KSGRRNFPAQ LLNFVEGSRQ NSNTIEVLLI NADMLRSKSM RNNDYDQTLI 

       190        200        210        220        230        240 
GGTTSPIEAI QDTRPVVIID EPHRFPRDKA NYQSIEAIKP QVIIRFGATF PEVTTGKGSN 

       250        260        270        280        290        300 
KITKKDYYRK KPQFDLNAIE SFNNGLVKGI DIYYPNLTEE QAKNRYVVDS VKAKELVLKQ 

       310        320        330        340        350        360 
NSKSWILHVG DNLAEVDSGF EGDIEYAGSK MLSNELELEK GMTLIPGTYR STYQELIIKD 

       370        380        390        400        410        420 
AIDKHFEIEQ ANFLRANQRE NNVPRIKTLS LFFIDSITSY RQDDGWLKAT FERLLKEKLS 

       430        440        450        460        470        480 
RLVAEYEFKR LPREKEYLEF LRATQSSLAS DNQNVHAGYF GEDRGSGDES IQAEVDDILK 

       490        500        510        520        530        540 
NKEKLLSFKD ENGNWQTRRF LFSKWTLREG WDNPNVFVIA KLRTSGSDNS KIQEVGRGLR 

       550        560        570        580        590        600 
LPVDETGHRI QQDEWPTRLA FLIGYDEKDF AQKLIGEINS DAKLQLNEEK LTEDMIQLIV 

       610        620        630        640        650        660 
TKRKKVNPEF TDERLLEHLD NLGIINRKNE FKESIDIGGV QKSGFEWLVE LYPELNTNRL 

       670        680        690        700        710        720 
REGKVIDTKK HSIKVRVKLR KENWEKVKEL WQQFSNRYML EFQRIPETIS FMAEQIVGNH 

       730        740        750        760        770        780 
SLYEREVPMQ MKESLHASDD NESVVLREQE NEYQRIYLPG MAYGKFVKRI NIATGIPIKE 

       790        800        810        820        830        840 
IHANLFKLMK SSLKGDARYL SELSLNNIIR EFKKRFDEIF AQAYEYKKLD FQARTAIYNP 

       850        860        870        880        890        900 
EMDSFVDDIN AEVIGVNIDE QAQEDNRYLY ELPPMRYDSV TPERDLLRHG YNDKVTVFGK 

       910        920        930        940        950        960 
LPRRAIQVPK YTGGSTTPDF IYMIEKDNDS SVYVLVETKA ENMRLEDQRI IDIQKKFFDT 

       970        980 
LKEHHIEFIE ATSAQQVYSI IRKLSEE 

« Hide

References

« Hide 'large scale' references
[1]"Genome organization is not conserved between Bacillus cereus and Bacillus subtilis."
Oekstad O.A., Hegna I.K., Lindbaeck T., Rishovd A.-L., Kolstoe A.-B.
Microbiology 145:621-631(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1."
Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D.
Nucleic Acids Res. 32:977-988(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10987.
[3]"A type-III DNA restriction and modification system in Bacillus cereus?"
Hegna I.K., Karlstroem E.S., Lopez R., Kristensen T., Kolstoe A.-B.
Gene 114:149-150(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 410-821.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ007510 Genomic DNA. Translation: CAB40612.1.
AE017194 Genomic DNA. Translation: AAS39950.1.
X60713 Genomic DNA. Translation: CAA43125.1.
PIRJC1116. S15518.
RefSeqNP_977342.1. NC_003909.8.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING222523.BCE_1019.

Protein family/group databases

REBASE2841. BceSI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS39950; AAS39950; BCE_1019.
GeneID2750767.
KEGGbca:BCE_1019.
PATRIC18850934. VBIBacCer118379_0972.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3587.
HOGENOMHOG000218980.
KOK01156.
OMARANQREN.
OrthoDBEOG6MPWQ7.

Enzyme and pathway databases

BRENDA3.1.21.5. 648.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR006935. Helicase/UvrB_dom.
IPR027417. P-loop_NTPase.
IPR014883. VRR_NUC.
[Graphical view]
PfamPF04851. ResIII. 1 hit.
PF08774. VRR_NUC. 1 hit.
[Graphical view]
SMARTSM00990. VRR_NUC. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 4 hits.
ProtoNetSearch...

Entry information

Entry nameT3RE_BACC1
AccessionPrimary (citable) accession number: P25241
Secondary accession number(s): Q9XBI5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 24, 2004
Last modified: May 14, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries