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P25240 (MT57_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Modification methylase Eco57IB

Short name=M.Eco57IB
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase Eco57IB
Gene names
Name:eco57IBM
Synonyms:eco57IM
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length544 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence 5'-CTGAAG-3' in one strand and 5'-CTTCAG-3' in the other, causes specific methylation on A-5 on one strand, and protects the DNA from cleavage by the Eco57I endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Monomer.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 544544Modification methylase Eco57IB
PRO_0000087940

Sequences

Sequence LengthMass (Da)Tools
P25240 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 53A1D5D7337AB4FA

FASTA54462,013
        10         20         30         40         50         60 
MKFKADQTSQ KLRGGYYTPQ NLADYVTKWV LSKNPKTILE PSCGDGVFIQ AIANNGYNSN 

        70         80         90        100        110        120 
IELFCFELFD TEASKALERC KLNNFSNATI TEGDFLVWAN ECLKKNKQIF DGALGNPPFI 

       130        140        150        160        170        180 
RYQFLERNFQ EQAQLVFEHL DLKFTKHTNA WVPFLLSSLA LLKQGGRIGM VIPSEISHVM 

       190        200        210        220        230        240 
HAQSLRSYLG HVCSKIVIID PKEIWFEDTL QGAVILLAEK KQYPDEASQG VGIVSVSGFE 

       250        260        270        280        290        300 
FLQEDPNVLF NDTAGINGET VEGKWTKATL SIDELQLIKR VIAHPDVRKF KDIAKVDVGR 

       310        320        330        340        350        360 
YCDGANNYFL VDNETVKLYK LERFAHPMFG RSQHCPGIIY DEKQHIENQE KGLPTNFLYI 

       370        380        390        400        410        420 
DEEFEYLSKS VKNYIKLGEV EEYHKRYKCR IRKPWFKVPS VYSTEIGMLK RCHDAPRLIH 

       430        440        450        460        470        480 
NRVRAYTTDT AYRVSSTVTS TENLVCSFLN PITVITAELE GLFYGGGVLE LVPSEIEKLY 

       490        500        510        520        530        540 
ILIVEGLEHN VEELNLLIKD GQIERVIRQQ GSLILGTLGF TQEENEKLVE IGRSLEIEGY 


VSRV 

« Hide

References

[1]"Cloning and sequence analysis of the genes coding for Eco57I type IV restriction-modification enzymes."
Janulaitis A., Vaisvila R., Timinskas A., Klimasauskas S., Butkus V.
Nucleic Acids Res. 20:6051-6056(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: RFL57.
[2]"Purification and properties of the Eco57I restriction endonuclease and methylase -- prototypes of a new class (type IV)."
Janulaitis A., Petrusyte M., Maneliene Z., Klimasauskas S., Butkus V.
Nucleic Acids Res. 20:6043-6049(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74821 Genomic DNA. Translation: AAA23388.1.
X61122 Genomic DNA. Translation: CAA43433.1.
PIRS26425.

3D structure databases

ProteinModelPortalP25240.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE5234. M.Eco57I.

Proteomic databases

PRIDEP25240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMT57_ECOLX
AccessionPrimary (citable) accession number: P25240
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries