P25240 (MT57_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase Eco57IB
Short name=M.Eco57IB
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase Eco57IB

Gene names

Name:	eco57IBM
Synonyms:	eco57IM

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence 5'-CTGAAG-3' in one strand and 5'-CTTCAG-3' in the other, causes specific methylation on A-5 on one strand, and protects the DNA from cleavage by the Eco57I endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Modification methylase Eco57IB		PRO_0000087940

Sequences

	Sequence		Length	Mass (Da)
	P25240 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 53A1D5D7337AB4FA	FASTA	544	62,013
	10 20 30 40 50 60 MKFKADQTSQ KLRGGYYTPQ NLADYVTKWV LSKNPKTILE PSCGDGVFIQ AIANNGYNSN 70 80 90 100 110 120 IELFCFELFD TEASKALERC KLNNFSNATI TEGDFLVWAN ECLKKNKQIF DGALGNPPFI 130 140 150 160 170 180 RYQFLERNFQ EQAQLVFEHL DLKFTKHTNA WVPFLLSSLA LLKQGGRIGM VIPSEISHVM 190 200 210 220 230 240 HAQSLRSYLG HVCSKIVIID PKEIWFEDTL QGAVILLAEK KQYPDEASQG VGIVSVSGFE 250 260 270 280 290 300 FLQEDPNVLF NDTAGINGET VEGKWTKATL SIDELQLIKR VIAHPDVRKF KDIAKVDVGR 310 320 330 340 350 360 YCDGANNYFL VDNETVKLYK LERFAHPMFG RSQHCPGIIY DEKQHIENQE KGLPTNFLYI 370 380 390 400 410 420 DEEFEYLSKS VKNYIKLGEV EEYHKRYKCR IRKPWFKVPS VYSTEIGMLK RCHDAPRLIH 430 440 450 460 470 480 NRVRAYTTDT AYRVSSTVTS TENLVCSFLN PITVITAELE GLFYGGGVLE LVPSEIEKLY 490 500 510 520 530 540 ILIVEGLEHN VEELNLLIKD GQIERVIRQQ GSLILGTLGF TQEENEKLVE IGRSLEIEGY VSRV

References

[1]	"Cloning and sequence analysis of the genes coding for Eco57I type IV restriction-modification enzymes." Janulaitis A., Vaisvila R., Timinskas A., Klimasauskas S., Butkus V. Nucleic Acids Res. 20:6051-6056(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: RFL57.
[2]	"Purification and properties of the Eco57I restriction endonuclease and methylase -- prototypes of a new class (type IV)." Janulaitis A., Petrusyte M., Maneliene Z., Klimasauskas S., Butkus V. Nucleic Acids Res. 20:6043-6049(1992) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74821 Genomic DNA. Translation: AAA23388.1. X61122 Genomic DNA. Translation: CAA43433.1.
PIR	S26425.
3D structure databases
ProteinModelPortal	P25240.
ModBase	Search...
Protein family/group databases
REBASE	5234. M.Eco57I.
Proteomic databases
PRIDE	P25240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
Pfam	PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT57_ECOLX

Accession

Primary (citable) accession number: P25240

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program