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P25239 (T257_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type IIS restriction enzyme Eco57I

EC=3.1.21.4
Alternative name(s):
Endonuclease Eco57I

Including the following 1 domains:

  1. Adenine-specific methyltransferase activity Eco57IA
    Short name=M.Eco57IA
    EC=2.1.1.72
Gene names
Name:eco57IR
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length998 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequences CTGAAG and CTTCAG and cleaves respectively 22 bases after C-1 and 14 bases before C'-1. Also acts as a methylase that causes specific methylation on A-5 on one strand, the other strand being methylated by the Eco57IB methylase.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Monomer.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 998998Type IIS restriction enzyme Eco57I
PRO_0000077274

Experimental info

Sequence conflict421I → L in AAA23389. Ref.1
Sequence conflict2071R → G in AAA23389. Ref.1
Sequence conflict299 – 32123WSIIE…FSSDI → EHHRTIILPHKAHTLSLFSL LIF in AAA23389. Ref.1
Sequence conflict4011E → V in AAA23389. Ref.1
Sequence conflict6441F → N in AAA23389. Ref.1
Sequence conflict816 – 8238PNEWYRYG → LMMVQIR in AAA23389. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25239 [UniParc].

Last modified September 13, 2004. Version 2.
Checksum: EDB090A7FBFFCC93

FASTA998117,087
        10         20         30         40         50         60 
MNKKDQLQRL IDKYKSDIDY YRSARYNETQ LRTDFLDQLF LILGWDITNA AGKPTNEREV 

        70         80         90        100        110        120 
LVEEGLKARA GENTKKPDYT FRLFSERKFF LEAKKPSVDI STTIEPALQV RRYGFTAKLK 

       130        140        150        160        170        180 
ISVLSNFEYT AIYDCSNQVK ETDSVANSRI KLYHFTELVD KFDEINNLIG RESVYTGHFD 

       190        200        210        220        230        240 
NEWSEIENKI LRFSVDDLFL KQINDWRLLL ANEFLQIKNE LPEEKLNDLV QNYINSIVFL 

       250        260        270        280        290        300 
RVCEDRDLEE YETLYHFAQD KDFQSLVKKL KSSDKKYNSG LFSLEYIDEL LSNANSCIWS 

       310        320        330        340        350        360 
IIEQLYFPQS TYSFSVFSSD ILGNIYEIFL SEKVRIDELG NVKIQPKEEH IDRDVVTTPT 

       370        380        390        400        410        420 
HIVKEIIRNT VVEYCKGKSD IEILNSKFAD IACGSGAFII EAFQFIQDIL IDYYIQNDKS 

       430        440        450        460        470        480 
KLQQISEHTY KLKFEVKREI LCKCIYGIDK DYNATKACTF GLLLKLLEGE TTETIGKDTP 

       490        500        510        520        530        540 
ILPALDTNIL FGNSLIDSGD KVKQEDIFSI NPFDLTNYQF DVIVGNPPYM ATEHMNQLTP 

       550        560        570        580        590        600 
KELDIYKRKY KSAYKQFDKY FLFIERSIQI LKEYGYLGYI LPSRFIKVDA GKKLRKFLSE 

       610        620        630        640        650        660 
NKYLSKLISF GSHQVFKNKT TYTCLLFLNK ENHDNFSFYE VKDFKKWLTR EDKYLLSSTY 

       670        680        690        700        710        720 
QTSSLDSDTW VLEKKINDIL KLMFSKSEQL GNIVGKSNVA NGIQTSANKY YIHKEIKSEN 

       730        740        750        760        770        780 
GFIYFEYDGI EYHIEKELTR PYFETNRSGD DSFYTYKDVE PNSFVVYPYK KVGERIQFIE 

       790        800        810        820        830        840 
YDELKRQYPK LFEFLQVVKV HLNDKKRSIK PDPTGPNEWY RYGRSQALEN CDVDQKLIVG 

       850        860        870        880        890        900 
ILSNGYKYSI DNHRTFVSSG GTAGYSIINI PNNVRYSIYY IQAILTSKYL EWFASIYGDI 

       910        920        930        940        950        960 
FRGRFVARGT KVQTRMPIPT IDFDDPKQKE IHDTISSKQQ YLNKLYSQTQ KSADRDKIIF 

       970        980        990 
ERQFEQEKIQ MDYLIKNLFD LGDLDSEIPT VEDLYKNL 

« Hide

References

[1]"Cloning and sequence analysis of the genes coding for Eco57I type IV restriction-modification enzymes."
Janulaitis A., Vaisvila R., Timinskas A., Klimasauskas S., Butkus V.
Nucleic Acids Res. 20:6051-6056(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: RFL57.
[2]"Crystallization and preliminary crystallographic studies of a bifunctional restriction endonuclease Eco57I."
Tamulaitiene G., Grazulis S., Janulaitis A., Janowski R., Bujacz G., Jaskolski M.
Biochim. Biophys. Acta 1698:251-254(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS), SEQUENCE REVISION.
[3]"Purification and properties of the Eco57I restriction endonuclease and methylase -- prototypes of a new class (type IV)."
Janulaitis A., Petrusyte M., Maneliene Z., Klimasauskas S., Butkus V.
Nucleic Acids Res. 20:6043-6049(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74821 Genomic DNA. Translation: AAA23389.1.
X61122 Genomic DNA. Translation: CAA43434.3.
PIRS26426.

3D structure databases

ProteinModelPortalP25239.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE941. Eco57I.

Proteomic databases

PRIDEP25239.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
[Graphical view]
PfamPF07669. Eco57I. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT257_ECOLX
AccessionPrimary (citable) accession number: P25239
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 13, 2004
Last modified: April 16, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries