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Protein

Modification methylase KpnI

Gene

kpnIM

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3436 M.KpnI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase KpnI (EC:2.1.1.72)
Short name:
M.KpnI
Alternative name(s):
Adenine-specific methyltransferase KpnI
Gene namesi
Name:kpnIM
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879661 – 417Modification methylase KpnIAdd BLAST417

Structurei

3D structure databases

ProteinModelPortaliP25238
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR002295 D21N6_MeTrfase
IPR002941 DNA_methylase_N4/N6
IPR002052 DNA_methylase_N6_adenine_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01555 N6_N4_Mtase, 1 hit
PIRSFiPIRSF015855 TypeIII_Mtase_mKpnI, 1 hit
PRINTSiPR00506 D21N6MTFRASE
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P25238-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNHANEINK LSRELGLLSN YEFNMDELKN LSPLDSTSSS IYIGDNLTYL
60 70 80 90 100
QGLSKTSPKT IDFCYIDPPY NTGNKIIYHD NRKSVSSDIF GLHNEWMSFL
110 120 130 140 150
LPRLFHAHKM LKDTGIIAIS IDDYEFAHLK ILMDKIFGED NFIGNIVVCR
160 170 180 190 200
SKNGKGSKRN IASAHEYLLV YGKSDMAELS GQPDDKSLYD KVDCFGEYRI
210 220 230 240 250
DGMFRKKGDS SLRTDRPNMF YPLYFNPSTG EVQVEPELGL KTVYPIDSKG
260 270 280 290 300
IERRWLWSKE TARERSWELF ASKNGVVYVK NYSSSHKRIK VRTLWNDSSF
310 320 330 340 350
YTERATNEIT KIFGSKVFDT PKALNYIMSI INCMAKPDAL ILDFFAGSGT
360 370 380 390 400
TAHAAAVLNS LDGGSRKTIL MESNHPITKT HIAYKSGFRK ISDITISRLN
410
YVSDNFPDFK YKKIEII
Length:417
Mass (Da):47,577
Last modified:May 1, 1992 - v1
Checksum:i42BFAA6F772347BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76435 Genomic DNA Translation: AAA25090.1
X61796 Genomic DNA Translation: CAA43898.1
PIRiS34433
RefSeqiWP_004176757.1, NZ_NRJD01000017.1

Entry informationi

Entry nameiMTK1_KLEPN
AccessioniPrimary (citable) accession number: P25238
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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