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P25238

- MTK1_KLEPN

UniProt

P25238 - MTK1_KLEPN

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Protein
Modification methylase KpnI
Gene
kpnIM
Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3436. M.KpnI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase KpnI (EC:2.1.1.72)
Short name:
M.KpnI
Alternative name(s):
Adenine-specific methyltransferase KpnI
Gene namesi
Name:kpnIM
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Modification methylase KpnI
PRO_0000087966Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP25238.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFiPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25238-1 [UniParc]FASTAAdd to Basket

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MDNHANEINK LSRELGLLSN YEFNMDELKN LSPLDSTSSS IYIGDNLTYL    50
QGLSKTSPKT IDFCYIDPPY NTGNKIIYHD NRKSVSSDIF GLHNEWMSFL 100
LPRLFHAHKM LKDTGIIAIS IDDYEFAHLK ILMDKIFGED NFIGNIVVCR 150
SKNGKGSKRN IASAHEYLLV YGKSDMAELS GQPDDKSLYD KVDCFGEYRI 200
DGMFRKKGDS SLRTDRPNMF YPLYFNPSTG EVQVEPELGL KTVYPIDSKG 250
IERRWLWSKE TARERSWELF ASKNGVVYVK NYSSSHKRIK VRTLWNDSSF 300
YTERATNEIT KIFGSKVFDT PKALNYIMSI INCMAKPDAL ILDFFAGSGT 350
TAHAAAVLNS LDGGSRKTIL MESNHPITKT HIAYKSGFRK ISDITISRLN 400
YVSDNFPDFK YKKIEII 417
Length:417
Mass (Da):47,577
Last modified:May 1, 1992 - v1
Checksum:i42BFAA6F772347BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76435 Genomic DNA. Translation: AAA25090.1.
X61796 Genomic DNA. Translation: CAA43898.1.
PIRiS34433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76435 Genomic DNA. Translation: AAA25090.1 .
X61796 Genomic DNA. Translation: CAA43898.1 .
PIRi S34433.

3D structure databases

ProteinModelPortali P25238.
ModBasei Search...

Protein family/group databases

REBASEi 3436. M.KpnI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PIRSFi PIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSi PR00506. D21N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetic organization of the KpnI restriction-modification system."
    Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.
    Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: OK8.

Entry informationi

Entry nameiMTK1_KLEPN
AccessioniPrimary (citable) accession number: P25238
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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