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P25238

- MTK1_KLEPN

UniProt

P25238 - MTK1_KLEPN

Protein

Modification methylase KpnI

Gene

kpnIM

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3436. M.KpnI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase KpnI (EC:2.1.1.72)
    Short name:
    M.KpnI
    Alternative name(s):
    Adenine-specific methyltransferase KpnI
    Gene namesi
    Name:kpnIM
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 417417Modification methylase KpnIPRO_0000087966Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP25238.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR002295. D21N6_MeTrfase.
    IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
    PRINTSiPR00506. D21N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25238-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDNHANEINK LSRELGLLSN YEFNMDELKN LSPLDSTSSS IYIGDNLTYL    50
    QGLSKTSPKT IDFCYIDPPY NTGNKIIYHD NRKSVSSDIF GLHNEWMSFL 100
    LPRLFHAHKM LKDTGIIAIS IDDYEFAHLK ILMDKIFGED NFIGNIVVCR 150
    SKNGKGSKRN IASAHEYLLV YGKSDMAELS GQPDDKSLYD KVDCFGEYRI 200
    DGMFRKKGDS SLRTDRPNMF YPLYFNPSTG EVQVEPELGL KTVYPIDSKG 250
    IERRWLWSKE TARERSWELF ASKNGVVYVK NYSSSHKRIK VRTLWNDSSF 300
    YTERATNEIT KIFGSKVFDT PKALNYIMSI INCMAKPDAL ILDFFAGSGT 350
    TAHAAAVLNS LDGGSRKTIL MESNHPITKT HIAYKSGFRK ISDITISRLN 400
    YVSDNFPDFK YKKIEII 417
    Length:417
    Mass (Da):47,577
    Last modified:May 1, 1992 - v1
    Checksum:i42BFAA6F772347BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76435 Genomic DNA. Translation: AAA25090.1.
    X61796 Genomic DNA. Translation: CAA43898.1.
    PIRiS34433.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76435 Genomic DNA. Translation: AAA25090.1 .
    X61796 Genomic DNA. Translation: CAA43898.1 .
    PIRi S34433.

    3D structure databases

    ProteinModelPortali P25238.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3436. M.KpnI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR002295. D21N6_MeTrfase.
    IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
    PRINTSi PR00506. D21N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic organization of the KpnI restriction-modification system."
      Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.
      Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: OK8.

    Entry informationi

    Entry nameiMTK1_KLEPN
    AccessioniPrimary (citable) accession number: P25238
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3