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P25238

- MTK1_KLEPN

UniProt

P25238 - MTK1_KLEPN

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Protein

Modification methylase KpnI

Gene

kpnIM

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3436. M.KpnI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase KpnI (EC:2.1.1.72)
Short name:
M.KpnI
Alternative name(s):
Adenine-specific methyltransferase KpnI
Gene namesi
Name:kpnIM
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Modification methylase KpnIPRO_0000087966Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP25238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFiPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25238-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNHANEINK LSRELGLLSN YEFNMDELKN LSPLDSTSSS IYIGDNLTYL
60 70 80 90 100
QGLSKTSPKT IDFCYIDPPY NTGNKIIYHD NRKSVSSDIF GLHNEWMSFL
110 120 130 140 150
LPRLFHAHKM LKDTGIIAIS IDDYEFAHLK ILMDKIFGED NFIGNIVVCR
160 170 180 190 200
SKNGKGSKRN IASAHEYLLV YGKSDMAELS GQPDDKSLYD KVDCFGEYRI
210 220 230 240 250
DGMFRKKGDS SLRTDRPNMF YPLYFNPSTG EVQVEPELGL KTVYPIDSKG
260 270 280 290 300
IERRWLWSKE TARERSWELF ASKNGVVYVK NYSSSHKRIK VRTLWNDSSF
310 320 330 340 350
YTERATNEIT KIFGSKVFDT PKALNYIMSI INCMAKPDAL ILDFFAGSGT
360 370 380 390 400
TAHAAAVLNS LDGGSRKTIL MESNHPITKT HIAYKSGFRK ISDITISRLN
410
YVSDNFPDFK YKKIEII
Length:417
Mass (Da):47,577
Last modified:May 1, 1992 - v1
Checksum:i42BFAA6F772347BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76435 Genomic DNA. Translation: AAA25090.1.
X61796 Genomic DNA. Translation: CAA43898.1.
PIRiS34433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76435 Genomic DNA. Translation: AAA25090.1 .
X61796 Genomic DNA. Translation: CAA43898.1 .
PIRi S34433.

3D structure databases

ProteinModelPortali P25238.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3436. M.KpnI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PIRSFi PIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSi PR00506. D21N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetic organization of the KpnI restriction-modification system."
    Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.
    Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: OK8.

Entry informationi

Entry nameiMTK1_KLEPN
AccessioniPrimary (citable) accession number: P25238
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 1, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3