Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P25238 (MTK1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase KpnI

Short name=M.KpnI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase KpnI
Gene names
Name:kpnIM
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Modification methylase KpnI
PRO_0000087966

Sequences

Sequence LengthMass (Da)Tools
P25238 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 42BFAA6F772347BD

FASTA41747,577
        10         20         30         40         50         60 
MDNHANEINK LSRELGLLSN YEFNMDELKN LSPLDSTSSS IYIGDNLTYL QGLSKTSPKT 

        70         80         90        100        110        120 
IDFCYIDPPY NTGNKIIYHD NRKSVSSDIF GLHNEWMSFL LPRLFHAHKM LKDTGIIAIS 

       130        140        150        160        170        180 
IDDYEFAHLK ILMDKIFGED NFIGNIVVCR SKNGKGSKRN IASAHEYLLV YGKSDMAELS 

       190        200        210        220        230        240 
GQPDDKSLYD KVDCFGEYRI DGMFRKKGDS SLRTDRPNMF YPLYFNPSTG EVQVEPELGL 

       250        260        270        280        290        300 
KTVYPIDSKG IERRWLWSKE TARERSWELF ASKNGVVYVK NYSSSHKRIK VRTLWNDSSF 

       310        320        330        340        350        360 
YTERATNEIT KIFGSKVFDT PKALNYIMSI INCMAKPDAL ILDFFAGSGT TAHAAAVLNS 

       370        380        390        400        410 
LDGGSRKTIL MESNHPITKT HIAYKSGFRK ISDITISRLN YVSDNFPDFK YKKIEII 

« Hide

References

[1]"Genetic organization of the KpnI restriction-modification system."
Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.
Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: OK8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M76435 Genomic DNA. Translation: AAA25090.1.
X61796 Genomic DNA. Translation: CAA43898.1.
PIRS34433.

3D structure databases

ProteinModelPortalP25238.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3436. M.KpnI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSPR00506. D21N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTK1_KLEPN
AccessionPrimary (citable) accession number: P25238
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries