P25238 (MTK1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase KpnI
Short name=M.KpnI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase KpnI

Gene names

Name:

kpnIM

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Modification methylase KpnI		PRO_0000087966

Sequences

	Sequence		Length	Mass (Da)
	P25238 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 42BFAA6F772347BD	FASTA	417	47,577
	10 20 30 40 50 60 MDNHANEINK LSRELGLLSN YEFNMDELKN LSPLDSTSSS IYIGDNLTYL QGLSKTSPKT 70 80 90 100 110 120 IDFCYIDPPY NTGNKIIYHD NRKSVSSDIF GLHNEWMSFL LPRLFHAHKM LKDTGIIAIS 130 140 150 160 170 180 IDDYEFAHLK ILMDKIFGED NFIGNIVVCR SKNGKGSKRN IASAHEYLLV YGKSDMAELS 190 200 210 220 230 240 GQPDDKSLYD KVDCFGEYRI DGMFRKKGDS SLRTDRPNMF YPLYFNPSTG EVQVEPELGL 250 260 270 280 290 300 KTVYPIDSKG IERRWLWSKE TARERSWELF ASKNGVVYVK NYSSSHKRIK VRTLWNDSSF 310 320 330 340 350 360 YTERATNEIT KIFGSKVFDT PKALNYIMSI INCMAKPDAL ILDFFAGSGT TAHAAAVLNS 370 380 390 400 410 LDGGSRKTIL MESNHPITKT HIAYKSGFRK ISDITISRLN YVSDNFPDFK YKKIEII

References

[1]	"Genetic organization of the KpnI restriction-modification system." Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F. Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OK8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76435 Genomic DNA. Translation: AAA25090.1. X61796 Genomic DNA. Translation: CAA43898.1.
PIR	S34433.
3D structure databases
ProteinModelPortal	P25238.
ModBase	Search...
Protein family/group databases
REBASE	3436. M.KpnI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PIRSF	PIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTS	PR00506. D21N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTK1_KLEPN

Accession

Primary (citable) accession number: P25238

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program